Origin IGS: gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgaaaaaatgggtggaaacacttgccttgattaccttggtaatcattctacgtttactggtaaattttttcttcccagagattccctattgggggagatttttactgacagtaattttgggagtcttggtaatcgtctccctatattttatgaatcgaaaaatgcgtaggtaaagagtttgaaaatgaaaggaaaaaac Mask Tandem Repeat Region ================================================ gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Find is-nt database================================================ Query_seq: SMT_1192:SMT_1193|SMT_1192:SMT_1193:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:phosphoribosylglycinamide formyltransferase:<-<-:1151497..1151696 200 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1192:SMT_1193|SMT_1192:SMT_1193:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:phosphoribosylglycinamide formyltransferase:<-<-:1151497..1151696 200 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1192:SMT_1193|SMT_1192:SMT_1193:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:phosphoribosylglycinamide formyltransferase:<-<-:1151497..1151696 200 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 56 End: 154 ....................................................... M G R R L P R L P K L L S V K I S P N R E S L G R K N L P V N V E .............................................. Protein_Len: 53 Strand: - Start: 34 End: 192 ................................. A N K S E Y F I P L R N G L S G F N S D T F I E G L L S D R P L F F K G T F T S H N G L Y D L C T N G G M ........ Protein_Len: 57 Strand: - Start: 30 End: 200 ............................. R R M K R N M F Y L S V I V L V G L I V T L L F R G W Y P I E P F F F N V L L R L I I V L T I L A L T E V W K K M gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1192:SMT_1193|SMT_1192:SMT_1193:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:phosphoribosylglycinamide formyltransferase:<-<-:1151497..1151696 200 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Intra-Species Hit: Count: 1 Min: 1 Max: 200 Len: 200 Subject: smit_SMT_1192_SMT_1193|phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:phosphoribosylglycinamide formyltransferase|NEGATIVE:NEGATIVE|[1151497, 1151696]|200 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 200 Query Start:1 Query End:200 Subject Strand: POSITIVE Subject Start: 1 Subject End: 200 gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat gttttttcctttcattttcaaactctttacctacgcatttttcgattcataaaatatagggagacgattaccaagactcccaaaattactgtcagtaaaaatctcccccaatagggaatctctgggaagaaaaaatttaccagtaaacgtagaatgattaccaaggtaatcaaggcaagtgtttccacccattttttcat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUUUCCUUUCAUUUUCAAACUCUUUACCUACGCAUUUUUCGAUUCAUAAAAUAUAGGGAGACGAUUACCAAGACUCCCAAAAUUACUGUCAGUAAAAAUCUCCCCCAAUAGGGAAUCUCUGGGAAGAAAAAAUUUACCAGUAAACGUAGAAUGAUUACCAAGGUAAUCAAGGCAAGUGUUUCCACCCAUUUUUUCAU ...................((((.(((...(((((........(((((..........((((((...((((...(((.............))).))))...))))))........))))).((((..............))))....)))))..(((((((....)))))))....))).))))................ (-36.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGAAAAAAUGGGUGGAAACACUUGCCUUGAUUACCUUGGUAAUCAUUCUACGUUUACUGGUAAAUUUUUUCUUCCCAGAGAUUCCCUAUUGGGGGAGAUUUUUACUGACAGUAAUUUUGGGAGUCUUGGUAAUCGUCUCCCUAUAUUUUAUGAAUCGAAAAAUGCGUAGGUAAAGAGUUUGAAAAUGAAAGGAAAAAAC ..........(((((....))))).((((((((((....))))))..(((((((...((((.((........)).)))).(((((.......((((((((..((((((((.............)))..)))))..)))))))).........))))).......)))))))..................))))....... (-50.61) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 888 110 61 201 152 smit:SMT_1192|5end_phosphoribosylaminoimidazolecarboxamide_formyltransferase_/_IMP_cyclohydrolase_1151406..1151636_NEGATIVE 308 199 157 129 84 smit:SMT_1748|5end_anthranilate_synthase_component_I_1726685..1726915_POSITIVE 304 118 71 185 145 smit:SMT_1088|5end_hypothetical_protein_1024346..1024576_NEGATIVE 282 199 151 157 112 smit:SMT_788|5end_hypothetical_protein_700319..700549_NEGATIVE 258 125 97 223 195 smit:SMT_968|5end_K01990_ABC-2_type_transport_system_ATP-binding_protein_914015..914245_NEGATIVE 257 187 149 62 18 smit:SMT_1470|5end_K06188_aquaporin_Z_1452560..1452790_POSITIVE 251 73 35 65 20 smit:SMT_1031|5end_MSM_(multiple_sugar_metabolism)_operon_regulatory_protein_967887..968117_POSITIVE 251 128 103 73 46 smit:SMT_460|5end_6-phospho-beta-glucosidase_397819..398049_POSITIVE 248 198 156 58 16 smit:SMT_841|5end_dihydrodipicolinate_reductase_788025..788255_NEGATIVE 248 94 54 90 48 smit:SMT_547|5end_branched-chain_amino_acid_aminotransferase_463947..464177_POSITIVE 247 80 36 213 169 smit:SMT_488|5end_dCMP_deaminase_422956..423186_POSITIVE 244 182 148 51 23 smit:SMT_1917|5end_ATP-dependent_nuclease_subunit_B_1861765..1861995_NEGATIVE 244 119 71 82 27 smit:SMT_1823|5end_GTP-binding_protein_1794982..1795212_POSITIVE 243 110 61 110 53 smit:SMT_1181|5end_phosphotransferase_system,_mannose/fructose-specific_component_IIA_1139817..1140047_NEGATIVE 237 110 63 185 123 smit:SMT_839|5end_ABC_transporter,_ATP-binding_protein_786065..786295_NEGATIVE 235 189 143 176 132 smit:SMT_2165|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_2038681..2038911_POSITIVE 232 200 164 139 100 smit:SMT_358|5end_hypothetical_protein_293947..294177_POSITIVE 230 110 61 103 63 smit:SMT_1393|5end_hypothetical_protein_1369106..1369336_POSITIVE 230 115 71 222 173 smit:SMT_454|5end_hypothetical_protein_391289..391519_POSITIVE 228 42 10 124 86 smit:SMT_1250|5end_hypothetical_protein_1211345..1211575_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 282 199 151 144 99 smit:SMT_789|3end_hypothetical_protein_700332..700482_NEGATIVE 258 187 145 54 1 smit:SMT_1469|3end_GTP_pyrophosphokinase_1452632..1452782_POSITIVE 252 92 52 146 100 smit:SMT_1495|3end_hypothetical_protein_1480983..1481133_POSITIVE 244 119 71 79 24 smit:SMT_1822|3end_hypothetical_protein_1795059..1795209_POSITIVE 243 110 61 106 49 smit:SMT_1182|3end_K02796_PTS_system,_mannose-specific_IID_component_1139821..1139971_NEGATIVE 235 198 154 127 77 smit:SMT_2081|3end_glycosyltransferase_1981336..1981486_POSITIVE 230 110 61 103 63 smit:SMT_1392|3end_dihydroxyacetone_kinase_1369186..1369336_POSITIVE 228 42 10 140 102 smit:SMT_1251|3end_hypothetical_protein_1211329..1211479_NEGATIVE 228 96 57 91 43 smit:SMT_403|3end_two-component_sensor_kinase_YesM_333351..333501_POSITIVE 224 92 59 49 15 smit:SMT_628|3end_glucose-1-phosphate_adenylyltransferase,_GlgD_subunit_534050..534200_POSITIVE 223 127 87 150 115 smit:SMT_1245|3end_SOS_responce_UmuC_protein_1205637..1205787_POSITIVE 222 48 4 132 72 smit:SMT_1206|3end_similar_to_sensor_histidine_kinase_(AgrC_from_Staphylococcus)_1165697..1165847_NEGATIVE 221 197 158 148 91 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 221 66 22 55 9 smit:SMT_1026|3end_sucrose_phosphorylase_960873..961023_NEGATIVE 220 50 12 58 12 smit:SMT_2079|3end_single_strand_binding_protein_1980499..1980649_POSITIVE 220 80 34 77 24 smit:SMT_335|3end_glutamine_synthetase_275179..275329_POSITIVE 219 195 151 92 43 smit:SMT_2089|3end_dTDP-4-dehydrorhamnose_3,5-epimerase_related_enzyme_1985270..1985420_POSITIVE 219 184 144 75 32 smit:SMT_1212|3end_acyl_carrier_protein,_putative_1171424..1171574_NEGATIVE 219 45 14 50 24 smit:SMT_866|3end_50S_ribosomal_protein_L11_815809..815959_NEGATIVE 215 98 57 84 42 smit:SMT_190|3end_preprotein_translocase,_SecY_subunit_125675..125825_POSITIVE