Origin IGS: ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaacgttaaagggaagaaaacagtttctttcctttttttgctttaaaagcgaaacgaaacaagattaaaacgaacgtttatggtataatattagtaaataattcgcaaaagaggttgaaag Mask Tandem Repeat Region ================================================ ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Find is-nt database================================================ Query_seq: SMT_1191:SMT_1192|SMT_1191:SMT_1192:phosphoribosylamine--glycine ligase:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:<-<-:1149828..1149948 121 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1191:SMT_1192|SMT_1191:SMT_1192:phosphoribosylamine--glycine ligase:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:<-<-:1149828..1149948 121 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1191:SMT_1192|SMT_1191:SMT_1192:phosphoribosylamine--glycine ligase:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:<-<-:1149828..1149948 121 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Predict ORF larger than 30AA ================================================ ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1191:SMT_1192|SMT_1191:SMT_1192:phosphoribosylamine--glycine ligase:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase:<-<-:1149828..1149948 121 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Intra-Species Hit: Count: 1 Min: 1 Max: 121 Len: 121 Subject: smit_SMT_1191_SMT_1192|phosphoribosylamine--glycine ligase:phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase|NEGATIVE:NEGATIVE|[1149828,1149948]|121 HSP 1 e-value: 3.0E-50 bit: 198.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcnnnnnnnggaaagaaactgttttcttccctttaacgttc ctttcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcaaaaaaaggaaagaaactgttttcttccctttaacgttc Inter-species Hit: Count: 3 Min: 4 Max: 114 Len: 111 Subject: NC_008533_SPD_0057_SPD_0058|bifunctional purine biosynthesis protein PurH:phosphoribosylamine--glycine ligase|POSITIVE:POSITIVE|[55523,55643]|121 HSP 1 e-value: 2.0E-20 bit: 99.6 Len: 111 Query Start:4 Query End:114 Subject Strand: NEGATIVE Subject Start: 8 Subject End: 118 ...tcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcnnnnnnnggaaagaaactgttttcttcccttt....... ...tcaacctcttttgcgaattatttaccaacattatatcaaaaaagttcgttttaatcttgtttcagttagcattttaagccaaaaaaggaaagaaactgttttcttcccttt....... Subject: NC_003098_spr0051_spr0052|bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:phosphoribosylamine--glycine ligase|POSITIVE:POSITIVE|[55523,55643]|121 HSP 1 e-value: 2.0E-20 bit: 99.6 Len: 111 Query Start:4 Query End:114 Subject Strand: NEGATIVE Subject Start: 8 Subject End: 118 ...tcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcnnnnnnnggaaagaaactgttttcttcccttt....... ...tcaacctcttttgcgaattatttaccaacattatatcaaaaaagttcgttttaatcttgtttcagttagcattttaagccaaaaaaggaaagaaactgttttcttcccttt....... Subject: NC_003028_SP_0050_SP_0051|bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase:phosphoribosylamine--glycine ligase|POSITIVE:POSITIVE|[54619,54739]|121 HSP 1 e-value: 5.0E-18 bit: 91.7 Len: 111 Query Start:4 Query End:114 Subject Strand: NEGATIVE Subject Start: 8 Subject End: 118 ...tcaacctcttttgcgaattatttactaatattataccataaacgttcgttttaatcttgtttcgtttcgcttttaaagcnnnnnnnggaaagaaactgttttcttcccttt....... ...tcaacctcttttgcgaattatttaccaacattatatcaaaaaagttcgttttaatcttgtttcagttagtattttaagccaaaaaaggaaagaaactgttttcttcccttt....... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAACCUCUUUUGCGAAUUAUUUACUAAUAUUAUACCAUAAACGUUCGUUUUAAUCUUGUUUCGUUUCGCUUUUAAAGCAAAAAAAGGAAAGAAACUGUUUUCUUCCCUUU .......((((.(((((......((.((.((((......((((....)))))))).)))).....)))))....)))).....(((((.((((((....)))))).))))) (-13.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGGGAAGAAAACAGUUUCUUUCCUUUUUUUGCUUUAAAAGCGAAACGAAACAAGAUUAAAACGAACGUUUAUGGUAUAAUAUUAGUAAAUAAUUCGCAAAAGAGGUUGA ((((((((((((....))))))))))))....(((((....(((((..............((((....)))).((.((......)).)).....)))))....)))))... (-19.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 762 110 61 204 155 smit:SMT_1191|5end_phosphoribosylamine--glycine_ligase_1149737..1149967_NEGATIVE 628 80 32 173 125 smit:SMT_2076|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1978592..1978822_NEGATIVE 298 49 2 142 96 smit:SMT_1600|5end_malolactic_enzyme_1589941..1590171_POSITIVE 279 65 23 202 165 smit:SMT_1776|5end_K07707_two-component_system,_AgrA_family,_response_regulator_AgrA_1752407..1752637_NEGATIVE 244 105 62 228 187 smit:SMT_933|5end_ATP-dependent_RNA_helicase_885631..885861_NEGATIVE 241 71 36 115 78 smit:SMT_1093|5end_K03073_preprotein_translocase_SecE_subunit_1033926..1034156_NEGATIVE 235 84 41 75 22 smit:SMT_1480|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1462043..1462273_POSITIVE 231 75 31 168 123 smit:SMT_1605|5end_hypothetical_protein_1595406..1595636_POSITIVE 228 50 5 148 101 smit:SMT_647|5end_DNA_gyrase,_A_subunit_554170..554400_NEGATIVE 216 103 64 129 92 smit:SMT_1679|5end_hypothetical_protein_1662998..1663228_POSITIVE 211 79 36 195 154 smit:SMT_1930|5end_peptide_chain_release_factor_1_1870765..1870995_NEGATIVE 211 99 56 217 174 smit:SMT_215|5end_leucyl-tRNA_synthetase_147045..147275_POSITIVE 210 69 44 109 81 smit:SMT_268|5end_penicillin-binding_protein_2X_205471..205701_POSITIVE 209 98 59 157 116 smit:SMT_602|5end_5'-methylthioadenosine_nucleosidase_/_S-adenosylhomocysteine_nucleosidase_517192..517422_POSITIVE 205 79 36 109 62 smit:SMT_1147|5end_hypothetical_protein_1092144..1092374_NEGATIVE 204 80 43 212 178 smit:SMT_2056|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1966323..1966553_POSITIVE 204 80 43 212 178 smit:SMT_1892|5end_hypothetical_protein_1845595..1845825_POSITIVE 203 74 36 125 88 smit:SMT_1812|5end_K07485_transposase_1786418..1786648_POSITIVE 200 29 1 229 203 smit:SMT_2075|5end_phosphoribosylamine--glycine_ligase_1978485..1978715_NEGATIVE 200 108 66 89 39 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 218 106 69 56 16 smit:SMT_950|3end_UDP-glucose_4-epimerase_897677..897827_NEGATIVE 216 103 64 42 5 smit:SMT_1678|3end_inorganic_pyrophosphatase_1662991..1663141_POSITIVE 214 76 45 144 111 smit:SMT_570|3end_hypothetical_protein_489179..489329_POSITIVE 211 108 61 76 25 smit:SMT_1648|3end_hypothetical_protein_1634883..1635033_POSITIVE 210 69 44 105 77 smit:SMT_267|3end_putative_cell_division_protein_205547..205697_POSITIVE 209 98 59 140 99 smit:SMT_601|3end_hypothetical_protein_517255..517405_POSITIVE 206 69 34 145 109 smit:SMT_246|3end_alcohol_dehydrogenase_182198..182348_NEGATIVE 205 79 36 131 84 smit:SMT_1148|3end_aspartyl-tRNA_synthetase_1092122..1092272_NEGATIVE 204 80 43 147 113 smit:SMT_2105|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1996984..1997134_POSITIVE 202 67 21 114 66 smit:SMT_1614|3end_K08998_hypothetical_protein_1601652..1601802_POSITIVE 200 108 66 81 31 smit:SMT_388|3end_branched-chain_amino_acid_transport_system_II_carrier_protein_321851..322001_POSITIVE 199 70 36 142 111 smit:SMT_1034|3end_K05896_segregation_and_condensation_protein_A_976768..976918_NEGATIVE 199 110 63 70 24 smit:SMT_332|3end_phosphoglycerate_kinase_272701..272851_POSITIVE 197 49 1 117 65 smit:SMT_1582|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1574000..1574150_POSITIVE 197 49 1 117 65 smit:SMT_285|3end_polysaccharide_polymerase_228692..228842_POSITIVE 196 108 71 48 13 smit:SMT_1982|3end_conserved_hypothetical_protein_1913412..1913562_POSITIVE 196 109 64 82 38 smit:SMT_1074|3end_catabolite_control_protein_A_1012275..1012425_NEGATIVE 193 89 42 83 49 smit:SMT_1237|3end_transposase,_TnpA_1198631..1198781_POSITIVE 192 90 42 130 84 smit:SMT_1006|3end_6-pyruvoyl_tetrahydrobiopterin_synthase,_putative_947122..947272_POSITIVE 192 106 61 144 94 smit:SMT_692|3end_hypothetical_protein_603008..603158_NEGATIVE