Origin IGS: aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttctaattagattttctgacaagaataaggagtaggatatcatatccgctccttttctttttgggttgagataagagtcatcatagcattggttaaaaattttcggtggttttattgtcaattctctgtaaatttttctcgttctaatcccatatagacaagatggcagaatcataatctataagttgtctggcttgactttcttgatgggaattatataatagatgtaataaaaataattacaaccgagaaagaggaccattt Mask Tandem Repeat Region ================================================ aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Find is-nt database================================================ Query_seq: SMT_1171:SMT_1172|SMT_1171:SMT_1172:hypothetical protein:hypothetical protein:<-<-:1119170..1119433 264 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1171:SMT_1172|SMT_1171:SMT_1172:hypothetical protein:hypothetical protein:<-<-:1119170..1119433 264 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1171:SMT_1172|SMT_1171:SMT_1172:hypothetical protein:hypothetical protein:<-<-:1119170..1119433 264 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 87 End: 263 ...................................................................................... M M I L P S C L Y G I R T R K I Y R E L T I K P P K I F N Q C Y D D S Y L N P K R K G A D M I S Y S L F L S E N L I R . Protein_Len: 37 Strand: - Start: 111 End: 221 .............................................................................................................. I P N S R S F N V S F Q C Y F W R F N K V L A I I V R I E V W F S F S R M ........................................... aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1171:SMT_1172|SMT_1171:SMT_1172:hypothetical protein:hypothetical protein:<-<-:1119170..1119433 264 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Intra-Species Hit: Count: 1 Min: 1 Max: 264 Len: 264 Subject: smit_SMT_1171_SMT_1172|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1119170,1119433]|264 HSP 1 e-value: 1.0E-148 bit: 523.0 Len: 264 Query Start:1 Query End:264 Subject Strand: POSITIVE Subject Start: 1 Subject End: 264 aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa aaatggtcctctttctcggttgtaattatttttattacatctattatataattcccatcaagaaagtcaagccagacaacttatagattatgattctgccatcttgtctatatgggattagaacgagaaaaatttacagagaattgacaataaaaccaccgaaaatttttaaccaatgctatgatgactcttatctcaacccaaaaagaaaaggagcggatatgatatcctactccttattcttgtcagaaaatctaattagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUGGUCCUCUUUCUCGGUUGUAAUUAUUUUUAUUACAUCUAUUAUAUAAUUCCCAUCAAGAAAGUCAAGCCAGACAACUUAUAGAUUAUGAUUCUGCCAUCUUGUCUAUAUGGGAUUAGAACGAGAAAAAUUUACAGAGAAUUGACAAUAAAACCACCGAAAAUUUUUAACCAAUGCUAUGAUGACUCUUAUCUCAACCCAAAAAGAAAAGGAGCGGAUAUGAUAUCCUACUCCUUAUUCUUGUCAGAAAAUCUAAUUAGAA ...........(((((((..((((((.......)))))).............((((.........(((......)))....(((((((...(((......)))..))))))).))))......))))))).......(((...(((((..................................(((((...)))))..........(((((((((((.(((((...)))))..)))))).))))))))))....)))........ (-47.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUAAUUAGAUUUUCUGACAAGAAUAAGGAGUAGGAUAUCAUAUCCGCUCCUUUUCUUUUUGGGUUGAGAUAAGAGUCAUCAUAGCAUUGGUUAAAAAUUUUCGGUGGUUUUAUUGUCAAUUCUCUGUAAAUUUUUCUCGUUCUAAUCCCAUAUAGACAAGAUGGCAGAAUCAUAAUCUAUAAGUUGUCUGGCUUGACUUUCUUGAUGGGAAUUAUAUAAUAGAUGUAAUAAAAAUAAUUACAACCGAGAAAGAGGACCAUUU ......((((.....)))).(((((.(((((((.(((((...)))))))))))))))))...((((((((((((((.(((((......................)))))))))))).)))))))...((...(((((((((......((((((..(((((((..(((((................)))))...))))....)))..)))))).............((((((.......))))))..)))))))))..))..... (-57.24) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 858 100 51 191 142 smit:SMT_1171|5end_hypothetical_protein_1119079..1119309_NEGATIVE 304 224 188 133 98 smit:SMT_1528|5end_K03310_alanine_or_glycine:cation_symporter,_AGCS_family_1512667..1512897_NEGATIVE 286 108 65 207 165 smit:SMT_118|5end_K02026_multiple_sugar_transport_system_permease_protein_69430..69660_POSITIVE 284 246 201 201 159 smit:SMT_308|5end_trans-2-enoyl-ACP_reductase_II_249439..249669_POSITIVE 262 109 65 221 163 smit:SMT_2106|5end_unknown,_predicted_membrane_protein_(TMS5)_1997163..1997393_POSITIVE 261 93 51 66 14 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 258 110 67 177 127 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 248 94 52 79 37 smit:SMT_774|5end_hypothetical_protein_685231..685461_NEGATIVE 248 220 177 134 86 smit:SMT_364|5end_ribosome-binding_factor_A_300923..301153_POSITIVE 246 109 62 230 188 smit:SMT_425|5end_hypothetical_cytosolic_protein_368114..368344_POSITIVE 244 109 65 222 163 smit:SMT_1771|5end_unknown,_predicted_membrane_protein_(TMS5)_1747437..1747667_POSITIVE 242 107 70 53 8 smit:SMT_1455|5end_deoxyribose-phosphate_aldolase_1438535..1438765_NEGATIVE 238 218 178 66 18 smit:SMT_1612|5end_K06024_segregation_and_condensation_protein_B_1600052..1600282_POSITIVE 237 250 211 170 131 smit:SMT_1052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_991821..992051_POSITIVE 236 250 212 225 183 smit:SMT_840|5end_tRNA_nucleotidyltransferase_(CCA-adding_enzyme)_787261..787491_NEGATIVE 233 22 3 40 17 smit:SMT_893|5end_leucine_rich_protein_841311..841541_NEGATIVE 232 243 207 213 172 smit:SMT_1809|5end_MutT/NUDIX_family_protein_1784602..1784832_POSITIVE 229 99 54 74 20 smit:SMT_1918|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1861935..1862165_NEGATIVE 229 122 85 167 124 smit:SMT_1414|5end_hypothetical_protein_1398446..1398676_NEGATIVE 228 105 65 93 43 smit:SMT_1466|5end_endopeptidase_O_1446962..1447192_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 246 201 83 41 smit:SMT_307|3end_K02078_acyl_carrier_protein_249401..249551_POSITIVE 267 140 94 147 104 smit:SMT_1580|3end_MutT/NUDIX_family_protein_1573153..1573303_POSITIVE 248 94 52 69 27 smit:SMT_775|3end_alpha-acetolactate_decarboxylase_685241..685391_NEGATIVE 246 109 62 144 102 smit:SMT_424|3end_transcriptional_regulatory_protein_368108..368258_POSITIVE 243 220 171 85 40 smit:SMT_363|3end_K02519_translation_initiation_factor_IF-2_300751..300901_POSITIVE 233 108 62 126 65 smit:SMT_1487|3end_hypothetical_protein_1467076..1467226_POSITIVE 233 22 3 36 13 smit:SMT_894|3end_hypothetical_protein_841315..841465_NEGATIVE 232 243 207 83 42 smit:SMT_1808|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1784552..1784702_POSITIVE 224 247 204 99 40 smit:SMT_1294|3end_K02049_sulfonate/nitrate/taurine_transport_system_ATP-binding_protein_1256791..1256941_POSITIVE 224 220 177 48 9 smit:SMT_652|3end_transporter,_putative_562037..562187_NEGATIVE 224 248 201 103 55 smit:SMT_533|3end_RNA_binding_protein_453537..453687_POSITIVE 224 248 201 103 55 smit:SMT_530|3end_RNA_binding_protein_452638..452788_POSITIVE 224 248 201 103 55 smit:SMT_526|3end_RNA_binding_protein_451468..451618_POSITIVE 224 248 201 103 55 smit:SMT_510|3end_hypothetical_protein_439357..439507_POSITIVE 222 235 197 40 1 smit:SMT_1529|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1512776..1512926_NEGATIVE 220 248 207 51 10 smit:SMT_1930|3end_peptide_chain_release_factor_1_1869785..1869935_NEGATIVE 220 248 207 146 105 smit:SMT_1929|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1869690..1869840_NEGATIVE 217 127 96 43 8 smit:SMT_1027|3end_K02026_multiple_sugar_transport_system_permease_protein_962641..962791_NEGATIVE 215 120 71 139 96 smit:SMT_137|3end_hypothetical_protein_88137..88287_NEGATIVE 214 248 207 101 67 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE