Origin IGS: tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaattaannnnnccgctcaggcctgcgggaacagtttattctaagacaatagaaaggaagtcatttgtacgaattgcaaaaaattaggattaaaaacagtcctgacctagaaaattatagata Mask Tandem Repeat Region ================================================ tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Find is-nt database================================================ Query_seq: SMT_1034:SMT_1035|SMT_1034:SMT_1035:K05896 segregation and condensation protein A:alpha-galactosidase:<-<-:977398..977521 124 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1034:SMT_1035|SMT_1034:SMT_1035:K05896 segregation and condensation protein A:alpha-galactosidase:<-<-:977398..977521 124 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1034:SMT_1035|SMT_1034:SMT_1035:K05896 segregation and condensation protein A:alpha-galactosidase:<-<-:977398..977521 124 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Predict ORF larger than 30AA ================================================ tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1034:SMT_1035|SMT_1034:SMT_1035:K05896 segregation and condensation protein A:alpha-galactosidase:<-<-:977398..977521 124 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagcggnnnnnttaattaa Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: smit_SMT_1034_SMT_1035|K05896 segregation and condensation protein A:alpha-galactosidase|NEGATIVE:NEGATIVE|[977398, 977521]|124 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagc............... tatctataattttctaggtcaggactgtttttaatcctaattttttgcaattcgtacaaatgacttcctttctattgtcttagaataaactgttcccgcaggcctgagc............... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCUAUAAUUUUCUAGGUCAGGACUGUUUUUAAUCCUAAUUUUUUGCAAUUCGUACAAAUGACUUCCUUUCUAUUGUCUUAGAAUAAACUGUUCCCGCAGGCCUGAGC ................(.(((((.((((.......................((((....))))......(((((......)))))............)))).))))).) (-15.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCAGGCCUGCGGGAACAGUUUAUUCUAAGACAAUAGAAAGGAAGUCAUUUGUACGAAUUGCAAAAAAUUAGGAUUAAAAACAGUCCUGACCUAGAAAAUUAUAGAUA ..(((((....(((((...((((((((((((((............))).((((((.....))))))...)))))))...))))..))))).))).))............ (-18.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 827 100 51 191 142 smit:SMT_1034|5end_K05896_segregation_and_condensation_protein_A_977307..977537_NEGATIVE 635 60 11 154 102 smit:SMT_1611|5end_K05896_segregation_and_condensation_protein_A_1599305..1599535_POSITIVE 292 80 36 117 67 smit:SMT_1111|5end_hypothetical_protein_1051409..1051639_NEGATIVE 247 84 41 82 38 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 238 47 11 140 103 smit:SMT_1590|5end_DNA-entry_nuclease_1578565..1578795_POSITIVE 238 76 43 55 12 smit:SMT_665|5end_NAD+_kinase_576745..576975_NEGATIVE 237 74 33 106 67 smit:SMT_1076|5end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_/_dihydroneopterin_aldolase_1014813..1015043_NEGATIVE 232 99 51 143 87 smit:SMT_1711|5end_Xaa-Pro_dipeptidase_1697494..1697724_NEGATIVE 231 60 11 163 119 smit:SMT_1604|5end_K05833_putative_ABC_transport_system_ATP-binding_protein_1594280..1594510_POSITIVE 231 68 26 148 108 smit:SMT_1278|5end_30S_ribosomal_protein_S2_1241473..1241703_POSITIVE 231 99 51 137 87 smit:SMT_1017|5end_transcriptional_regulator,_MerR_family_954448..954678_NEGATIVE 225 68 21 101 48 smit:SMT_167|5end_acetyltransferase,_GNAT_family_113171..113401_POSITIVE 219 47 9 152 116 smit:SMT_1645|5end_hypothetical_protein_1631738..1631968_POSITIVE 219 59 17 95 56 smit:SMT_818|5end_cystathionine_gamma-synthase_729801..730031_NEGATIVE 219 69 25 171 119 smit:SMT_548|5end_K02035_peptide/nickel_transport_system_substrate-binding_protein_464992..465222_POSITIVE 210 98 60 136 90 smit:SMT_1931|5end_23S_rRNA_methyltransferase_1870978..1871208_NEGATIVE 209 99 60 218 172 smit:SMT_1880|5end_hypothetical_protein_1836854..1837084_POSITIVE 209 54 14 170 137 smit:SMT_973|5end_signal_transduction_histidine_kinase_922177..922407_NEGATIVE 209 99 51 160 100 smit:SMT_356|5end_hypothetical_protein_292394..292624_POSITIVE 207 85 48 200 165 smit:SMT_1378|5end_DNA_polymerase_III,_delta'_subunit_1356803..1357033_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 600 57 11 151 102 smit:SMT_1610|3end_tyrosine_recombinase_1599385..1599535_POSITIVE 292 80 36 139 89 smit:SMT_1112|3end_K02248_competence_protein_ComGF_1051387..1051537_NEGATIVE 238 47 11 101 64 smit:SMT_1589|3end_hypothetical_protein_1578606..1578756_POSITIVE 238 76 43 71 28 smit:SMT_666|3end_GTP_pyrophosphokinase-like_protein_576729..576879_NEGATIVE 237 74 33 63 24 smit:SMT_1077|3end_GTP_cyclohydrolase_I_1014856..1015006_NEGATIVE 232 99 51 150 94 smit:SMT_1712|3end_excinuclease_ABC_subunit_A_1697487..1697637_NEGATIVE 231 60 11 150 106 smit:SMT_1603|3end_K05832_putative_ABC_transport_system_permease_protein_1594347..1594497_POSITIVE 225 68 21 57 4 smit:SMT_166|3end_hypothetical_protein_113207..113357_POSITIVE 219 69 25 148 96 smit:SMT_547|3end_branched-chain_amino_acid_aminotransferase_465049..465199_POSITIVE 209 48 3 115 69 smit:SMT_341|3end_ThiJ/PfpI_family_protein_281681..281831_POSITIVE 207 38 3 50 6 smit:SMT_541|3end_K03697_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpE_457120..457270_NEGATIVE 205 86 41 117 72 smit:SMT_1995|3end_phosphoglycerate_mutase_1920535..1920685_NEGATIVE 205 100 51 130 87 smit:SMT_1458|3end_K02040_phosphate_transport_system_substrate-binding_protein_1440602..1440752_POSITIVE 205 78 38 124 84 smit:SMT_725|3end_hypothetical_protein_630050..630200_NEGATIVE 202 96 53 123 84 smit:SMT_1544|3end_mevalonate_kinase_1528006..1528156_NEGATIVE 201 99 63 117 79 smit:SMT_1131|3end_threonine_synthase_1065271..1065421_NEGATIVE 200 58 11 149 97 smit:SMT_1403|3end_dihydrolipoamide_dehydrogenase_1380987..1381137_POSITIVE 200 50 2 149 99 smit:SMT_261|3end_ImpB/MucB/SamB_family_protein_201655..201805_POSITIVE 199 69 21 83 30 smit:SMT_1150|3end_hypothetical_protein_1094288..1094438_NEGATIVE 198 80 36 115 69 smit:SMT_1415|3end_carboxynorspermidine_decarboxylase_1398443..1398593_NEGATIVE