Origin IGS: nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtgatgttcttagctttttggcgaaatcatgggaacgtttaaggattcgctgtaaaatggctggggcatacctgacgcttcaatggttacgaccttggtaatgccagcagaagcaatttttccgcaaaaccttacaatctctcnnnnn Mask Tandem Repeat Region ================================================ nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Find is-nt database================================================ Query_seq: SMT_1010:SMT_1011|SMT_1010:SMT_1011:hypothetical protein:xanthine phosphoribosyltransferase:->->:948822..948970 149 nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1010:SMT_1011|SMT_1010:SMT_1011:hypothetical protein:xanthine phosphoribosyltransferase:->->:948822..948970 149 nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1010:SMT_1011|SMT_1010:SMT_1011:hypothetical protein:xanthine phosphoribosyltransferase:->->:948822..948970 149 nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 11 End: 136 .......... M V R F C G K I A S A G I T K V V T I E A S G M P Q P F Y S E S L N V P M I S P K S ............. nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1010:SMT_1011|SMT_1010:SMT_1011:hypothetical protein:xanthine phosphoribosyltransferase:->->:948822..948970 149 nnnnngagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Intra-Species Hit: Count: 1 Min: 6 Max: 149 Len: 144 Subject: smit_SMT_1010_SMT_1011|hypothetical protein:xanthine phosphoribosyltransferase|POSITIVE:POSITIVE|[948822, 948970]|149 HSP 1 e-value: 2.0E-76 bit: 285.0 Len: 144 Query Start:6 Query End:149 Subject Strand: POSITIVE Subject Start: 6 Subject End: 149 .....gagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact .....gagagattgtaaggttttgcggaaaaattgcttctgctggcattaccaaggtcgtaaccattgaagcgtcaggtatgccccagccattttacagcgaatccttaaacgttcccatgatttcgccaaaaagctaagaacatcact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAGAUUGUAAGGUUUUGCGGAAAAAUUGCUUCUGCUGGCAUUACCAAGGUCGUAACCAUUGAAGCGUCAGGUAUGCCCCAGCCAUUUUACAGCGAAUCCUUAAACGUUCCCAUGAUUUCGCCAAAAAGCUAAGAACAUCACU (.(((....(((((.(((((.(.(((((.(((......(((((.(((...(.(((..........))).).))))))))..))).))))).).))))).)))))....))).).(((((((((......))...))).)))).. (-30.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGAUGUUCUUAGCUUUUUGGCGAAAUCAUGGGAACGUUUAAGGAUUCGCUGUAAAAUGGCUGGGGCAUACCUGACGCUUCAAUGGUUACGACCUUGGUAAUGCCAGCAGAAGCAAUUUUUCCGCAAAACCUUACAAUCUCUC .((((((((((.((((.(((((((((.(((((....)))....)).))))))...))).))))))))))).......(((((..((((....((....))...))))...)))))........))).................. (-34.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 113 72 99 65 smit:SMT_1056|5end_K07738_ATP-cone_domain_protein_994842..995072_POSITIVE 334 79 32 212 158 smit:SMT_857|5end_endo-beta-N-acetylglucosaminidase_802491..802721_NEGATIVE 328 114 72 201 163 smit:SMT_1471|5end_hypothetical_protein_1453933..1454163_NEGATIVE 326 79 38 72 20 smit:SMT_1637|5end_hypothetical_protein_1623091..1623321_POSITIVE 320 95 52 102 45 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 318 78 36 97 39 smit:SMT_2010|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1929514..1929744_POSITIVE 306 86 41 200 154 smit:SMT_1129|5end_hypothetical_protein_1063926..1064156_NEGATIVE 301 79 52 37 5 smit:SMT_754|5end_3-dehydroquinate_synthase_659044..659274_NEGATIVE 300 86 45 126 77 smit:SMT_1864|5end_integral_membrane_protein_1826517..1826747_POSITIVE 300 128 83 75 35 smit:SMT_1181|5end_phosphotransferase_system,_mannose/fructose-specific_component_IIA_1139817..1140047_NEGATIVE 297 79 31 200 155 smit:SMT_2035|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1947167..1947397_POSITIVE 297 42 1 171 128 smit:SMT_585|5end_ATP_synthase_gamma_chain_501569..501799_POSITIVE 296 70 26 219 174 smit:SMT_939|5end_phosphomethylpyrimidine_kinase_892025..892255_NEGATIVE 292 107 68 72 21 smit:SMT_2145|5end_K05832_putative_ABC_transport_system_permease_protein_2022019..2022249_NEGATIVE 292 107 68 72 21 smit:SMT_1603|5end_K05832_putative_ABC_transport_system_permease_protein_1593374..1593604_POSITIVE 292 95 58 231 200 smit:SMT_568|5end_hypothetical_protein_485674..485904_POSITIVE 289 125 81 90 38 smit:SMT_1466|5end_endopeptidase_O_1446962..1447192_POSITIVE 289 109 65 226 188 smit:SMT_984|5end_transcriptional_regulator_Spx_930898..931128_NEGATIVE 288 57 16 202 151 smit:SMT_168|5end_anaerobic_ribonucleoside-triphosphate_reductase_activating_protein_113670..113900_POSITIVE 287 80 31 59 2 smit:SMT_1726|5end_tRNA_pseudouridine_synthase_B_1711495..1711725_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 79 38 88 36 smit:SMT_1636|3end_5-formyltetrahydrofolate_cyclo-ligase_family_protein_1623187..1623337_POSITIVE 300 86 45 85 36 smit:SMT_1863|3end_glutamyl_aminopeptidase_1826556..1826706_POSITIVE 300 128 83 71 31 smit:SMT_1182|3end_K02796_PTS_system,_mannose-specific_IID_component_1139821..1139971_NEGATIVE 292 107 68 60 9 smit:SMT_1602|3end_hypothetical_protein_1593442..1593592_POSITIVE 287 86 41 73 20 smit:SMT_1251|3end_hypothetical_protein_1211329..1211479_NEGATIVE 283 80 31 119 73 smit:SMT_1760|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1735743..1735893_NEGATIVE 278 100 57 123 71 smit:SMT_379|3end_acetyltransferase,_GNAT_family_316456..316606_POSITIVE 278 100 57 123 71 smit:SMT_370|3end_acetyltransferase,_GNAT_family_305328..305478_POSITIVE 277 95 58 54 3 smit:SMT_1806|3end_aminoacylase/N-acyl-L-amino_acid_amidohydrolase/hippurate_hydrolase_1781644..1781794_POSITIVE 276 58 19 143 101 smit:SMT_656|3end_bacteriocin_transport_accessory_protein,_putative_568419..568569_POSITIVE 273 77 36 143 102 smit:SMT_942|3end_hypothetical_protein_893528..893678_NEGATIVE 272 108 63 108 51 smit:SMT_603|3end_hypothetical_protein_518532..518682_POSITIVE 271 54 12 60 5 smit:SMT_1729|3end_exodeoxyribonuclease_VII_small_subunit_1714858..1715008_POSITIVE 271 78 37 151 111 smit:SMT_1581|3end_transcriptional_regulator,_biotin_repressor_family_1573120..1573270_NEGATIVE 270 78 32 52 16 smit:SMT_1067|3end_alanine_racemase_1006952..1007102_POSITIVE 268 86 47 148 99 smit:SMT_858|3end_triosephosphate_isomerase_802525..802675_NEGATIVE 267 74 31 53 15 smit:SMT_1480|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1462266..1462416_POSITIVE 266 109 65 66 19 smit:SMT_566|3end_K07282_poly-gamma-glutamate_synthesis_protein_(capsule_biosynthesis_protein)_484903..485053_POSITIVE 264 85 41 43 1 smit:SMT_1914|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1860324..1860474_POSITIVE 262 97 67 134 102 smit:SMT_1218|3end_DNA_polymerase_I_1176688..1176838_NEGATIVE