Origin IGS: taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttttgtaatgcgttgataaagaagaggttacatgatagatatgttttgaaaaattctttacattgttttcggcttctgtaaagtaagataatgggtaggaatttgtacaggtttgttttgcagtaggtgttgttatttagctttaattgtagtataaaataagctatatta Mask Tandem Repeat Region ================================================ taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Find is-nt database================================================ Query_seq: PI2152:PI2153|PI2152:PI2153:heptosyltransferase:hypothetical protein:->->:2099357..2099531 175 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2152:PI2153|PI2152:PI2153:heptosyltransferase:hypothetical protein:->->:2099357..2099531 175 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2152:PI2153|PI2152:PI2153:heptosyltransferase:hypothetical protein:->->:2099357..2099531 175 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 36 End: 173 ................................... M T T P T A K Q T C T N S Y P L S Y F T E A E N N V K N F S K H I Y H V T S S L S T H Y K T .. Protein_Len: 57 Strand: + Start: 5 End: 175 .... M A Y F I L Q L K L N N N T Y C K T N L Y K F L P I I L L Y R S R K Q C K E F F K T Y L S C N L F F I N A L Q N L taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 14 End: 43 .............GTTGTTATTTAGCTTTAATTGTAGTATAAA.................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2152:PI2153|PI2152:PI2153:heptosyltransferase:hypothetical protein:->->:2099357..2099531 175 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 175 Len: 175 Subject: pint_PI2152_PI2153|heptosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[2099357,2099531]|175 HSP 1 e-value: 8.0E-95 bit: 347.0 Len: 175 Query Start:1 Query End:175 Subject Strand: POSITIVE Subject Start: 1 Subject End: 175 taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta taatatagcttattttatactacaattaaagctaaataacaacacctactgcaaaacaaacctgtacaaattcctacccattatcttactttacagaagccgaaaacaatgtaaagaatttttcaaaacatatctatcatgtaacctcttctttatcaacgcattacaaaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUAGCUUAUUUUAUACUACAAUUAAAGCUAAAUAACAACACCUACUGCAAAACAAACCUGUACAAAUUCCUACCCAUUAUCUUACUUUACAGAAGCCGAAAACAAUGUAAAGAAUUUUUCAAAACAUAUCUAUCAUGUAACCUCUUCUUUAUCAACGCAUUACAAAACCUA .....((((((.................))))))...............(((....................................(((((((..............)))))))...........((((.......))))..................)))............ (-12.57) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUGUAAUGCGUUGAUAAAGAAGAGGUUACAUGAUAGAUAUGUUUUGAAAAAUUCUUUACAUUGUUUUCGGCUUCUGUAAAGUAAGAUAAUGGGUAGGAAUUUGUACAGGUUUGUUUUGCAGUAGGUGUUGUUAUUUAGCUUUAAUUGUAGUAUAAAAUAAGCUAUAUUA ...((((((((.((((....(((((.........((.(((((((.(((......((((((((..((((((((((.((....))...).)))))))))...))))))))..))).))))))).))...((((((....)))))).)))))..)))).))))))))........... (-30.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 170 134 150 114 pint:PI0575|5end_hypothetical_protein_519561..519791_POSITIVE 313 170 121 158 108 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 283 170 132 43 5 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 282 170 121 112 62 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 256 158 111 188 141 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 253 170 121 176 126 pint:PI2024|5end_hypothetical_protein_1978804..1979034_POSITIVE 241 170 129 43 2 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 233 170 121 188 138 pint:PI0949|5end_endonuclease_882264..882494_POSITIVE 230 148 101 131 71 pint:PI0887|5end_hypothetical_protein_817536..817766_POSITIVE 226 139 98 223 171 pint:PI0886|5end_hypothetical_protein_817640..817870_POSITIVE 222 154 115 106 66 pint:PI0538|5end_transcriptional_regulator_492561..492791_POSITIVE 219 126 84 83 43 pint:PI1172|5end_sensor_histidine_kinase_1091850..1092080_POSITIVE 216 159 111 119 71 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 215 118 76 60 25 pint:PI1043|5end_conserved_hypothetical_protein_960855..961085_POSITIVE 215 112 74 212 176 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 212 166 121 179 134 pint:PI1890|5end_cell_division_protein_FtsW_1832886..1833116_POSITIVE 209 170 121 150 100 pint:PI0653|5end_hypothetical_protein_596046..596276_POSITIVE 206 86 44 78 27 pint:PI2014|5end_conserved_hypothetical_protein_1971471..1971701_POSITIVE 204 117 73 114 49 pint:PI0740|5end_hypothetical_protein_672112..672342_POSITIVE 204 79 40 157 121 pint:PI0604|5end_O-succinylbenzoic_acid--CoA_ligase_550825..551055_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 413 170 121 124 74 pint:PI0069|3end_hypothetical_protein_67379..67529_POSITIVE 350 159 111 86 38 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 322 170 121 85 37 pint:PI0815|3end_hypothetical_protein_754677..754827_POSITIVE 307 170 137 36 3 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 256 158 111 73 26 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 253 170 121 124 74 pint:PI2023|3end_hypothetical_protein_1978832..1978982_POSITIVE 246 165 135 108 78 pint:PI0902|3end_hypothetical_protein_832580..832730_POSITIVE 241 170 129 97 56 pint:PI0118|3end_hypothetical_protein_111640..111790_POSITIVE 230 148 101 78 18 pint:PI0885|3end_hypothetical_protein_817563..817713_POSITIVE 227 170 134 146 110 pint:PI0926|3end_hypothetical_protein_857047..857197_POSITIVE 222 154 115 88 48 pint:PI0536|3end_hypothetical_protein_492623..492773_POSITIVE 215 112 74 110 74 pint:PI0928|3end_hypothetical_protein_860272..860422_POSITIVE 215 170 121 85 36 pint:PI0638|3end_hypothetical_protein_582402..582552_POSITIVE 215 170 121 80 31 pint:PI0637|3end_hypothetical_protein_582397..582547_POSITIVE 214 102 69 108 60 pint:PI0962|3end_phosphoglycerate_mutase_892702..892852_POSITIVE 207 150 101 105 55 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 206 86 44 82 31 pint:PI2012|3end_hypothetical_protein_1971555..1971705_POSITIVE 206 170 130 124 84 pint:PI1308|3end_hypothetical_protein_1223717..1223867_POSITIVE 205 168 129 56 17 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 204 117 73 83 18 pint:PI0739|3end_conserved_hypothetical_protein_672161..672311_POSITIVE