Origin IGS: aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttctgctcttttaatttttctgcaaatatagttcaaataaatcatatacacaaaataaaagcaaggcatttttatccgagaaagcctcaaagttgtaaaaaatacattaaattgatgtgccaatatggttaagaacgatgtgccgaacaaatggaagtaatgttataatcatctgttataatggcttgatttagcgcaatataatatcaaaagtgctgtttttattgcacaaatgcatatgttttgtgcatagatgagtgcttttttaagcaaaaaatttgcgtgtatgaaaaatttatctttcctttgcaaccgaattttagaatttagaaagttaatagtaaacattttaaaacttaacaatt Mask Tandem Repeat Region ================================================ aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Find is-nt database================================================ Query_seq: PI2150:PI2151|PI2150:PI2151:acyl carrier protein:conserved hypothetical protein:->->:2097346..2097712 367 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2150:PI2151|PI2150:PI2151:acyl carrier protein:conserved hypothetical protein:->->:2097346..2097712 367 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2150:PI2151|PI2150:PI2151:acyl carrier protein:conserved hypothetical protein:->->:2097346..2097712 367 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 40 End: 141 ....................................... M L K F G C K G K I N F S Y T Q I F C L K K H S S M H K T Y A F V Q .................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 3 End: 194 .. M L S F K M F T I N F L N S K I R L Q R K D K F F I H A N F L L K K A L I Y A Q N I C I C A I K T A L L I L Y C A K S S ............................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 263 End: 352 ...................................................................................................................................................................................................................................................................... M F Y N F E A F S D K N A L L L F C V Y D L F E L Y L Q K N ............... Protein_Len: 50 Strand: + Start: 216 End: 365 ....................................................................................................................................................................................................................... M F G T S F L T I L A H Q F N V F F T T L R L S R I K M P C F Y F V Y M I Y L N Y I C R K I K R A E .. Protein_Len: 34 Strand: - Start: 237 End: 338 ............................................................................................................................................................................................................................................ G Y Q C M L K I Y K K C S Q P K R P Y F H R A K I K H I H N I Q V M ............................. Protein_Len: 37 Strand: - Start: 2 End: 112 . N N L K L I N V I L K R F E L I R N C L F S L N K M C A F K K S L F A S M ............................................................................................................................................................................................................................................................... aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 20 End: 49 ...................GTTTACTATTAACTTTCTAAATTCTAAAAT.............................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2150:PI2151|PI2150:PI2151:acyl carrier protein:conserved hypothetical protein:->->:2097346..2097712 367 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Intra-Species Hit: Count: 1 Min: 1 Max: 367 Len: 367 Subject: pint_PI2150_PI2151|acyl carrier protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2097346,2097712]|367 HSP 1 e-value: 0.0 bit: 728.0 Len: 367 Query Start:1 Query End:367 Subject Strand: POSITIVE Subject Start: 1 Subject End: 367 aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat aattgttaagttttaaaatgtttactattaactttctaaattctaaaattcggttgcaaaggaaagataaatttttcatacacgcaaattttttgcttaaaaaagcactcatctatgcacaaaacatatgcatttgtgcaataaaaacagcacttttgatattatattgcgctaaatcaagccattataacagatgattataacattacttccatttgttcggcacatcgttcttaaccatattggcacatcaatttaatgtattttttacaactttgaggctttctcggataaaaatgccttgcttttattttgtgtatatgatttatttgaactatatttgcagaaaaattaaaagagcagaaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUGUUAAGUUUUAAAAUGUUUACUAUUAACUUUCUAAAUUCUAAAAUUCGGUUGCAAAGGAAAGAUAAAUUUUUCAUACACGCAAAUUUUUUGCUUAAAAAAGCACUCAUCUAUGCACAAAACAUAUGCAUUUGUGCAAUAAAAACAGCACUUUUGAUAUUAUAUUGCGCUAAAUCAAGCCAUUAUAACAGAUGAUUAUAACAUUACUUCCAUUUGUUCGGCACAUCGUUCUUAACCAUAUUGGCACAUCAAUUUAAUGUAUUUUUUACAACUUUGAGGCUUUCUCGGAUAAAAAUGCCUUGCUUUUAUUUUGUGUAUAUGAUUUAUUUGAACUAUAUUUGCAGAAAAAUUAAAAGAGCAGAAAU ................................(((((...((((..((((...(((((((.......(((((...(((((.(((((((.....(((((....))))).((((((.((((((((.........)))))))).....................(((((.((.(((......))))).))))).))))))........................((((...((((.(((.((.....(((...((((.....((((.....))))...)))).))).....)).))).))))...)))).....))))))).)))))...))))).......)))))))...))))...)))).))))). (-62.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUCUGCUCUUUUAAUUUUUCUGCAAAUAUAGUUCAAAUAAAUCAUAUACACAAAAUAAAAGCAAGGCAUUUUUAUCCGAGAAAGCCUCAAAGUUGUAAAAAAUACAUUAAAUUGAUGUGCCAAUAUGGUUAAGAACGAUGUGCCGAACAAAUGGAAGUAAUGUUAUAAUCAUCUGUUAUAAUGGCUUGAUUUAGCGCAAUAUAAUAUCAAAAGUGCUGUUUUUAUUGCACAAAUGCAUAUGUUUUGUGCAUAGAUGAGUGCUUUUUUAAGCAAAAAAUUUGCGUGUAUGAAAAAUUUAUCUUUCCUUUGCAACCGAAUUUUAGAAUUUAGAAAGUUAAUAGUAAACAUUUUAAAACUUAACAAUU .((((((.(((...(((((...((((((...((...((((...((((((((.((((.........((((.(((((....))))).))))....(((((((....((((((.....)))))).....(((((((..((((.((..(((......)))...)).)))).)))))))...))))))).((((((...(((((...............((((..........))))..(((((((.....)))))))......)))))...))))))......)))).))))))))...))))..))....))))))...)))))..)))...))))))(((((...(((.....)))....))))).... (-69.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 314 158 117 78 37 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 302 158 116 78 33 pint:PI1567|5end_hypothetical_protein_1489229..1489459_POSITIVE 296 249 213 167 118 pint:PI0959|5end_uridine_kinase_890495..890725_POSITIVE 285 158 112 209 159 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 283 251 212 76 34 pint:PI1078|5end_conserved_hypothetical_protein_999500..999730_POSITIVE 281 158 115 86 45 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 281 158 115 78 37 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 281 248 209 197 151 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 279 158 116 78 33 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 279 158 116 204 159 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 277 268 221 105 65 pint:PI0339|5end_hypothetical_protein_315735..315965_POSITIVE 276 249 203 211 170 pint:PI2155|5end_hypothetical_protein_2100664..2100894_POSITIVE 273 254 217 187 151 pint:PI1532|5end_uridylate_kinase_1451644..1451874_POSITIVE 273 318 273 129 70 pint:PI0372|5end_conserved_hypothetical_protein;_possible_ATPase_342011..342241_POSITIVE 266 268 221 105 65 pint:PI1632|5end_hypothetical_protein_1552819..1553049_POSITIVE 265 157 113 77 35 pint:PI1829|5end_hypothetical_protein_1769255..1769485_POSITIVE 262 247 209 166 113 pint:PI2044|5end_hypothetical_protein_1996999..1997229_POSITIVE 261 305 262 66 26 pint:PI1767|5end_fructokinase,_pfkB_family_1694285..1694515_POSITIVE 259 302 271 88 56 pint:PI0687|5end_hypothetical_protein_624256..624486_POSITIVE 258 158 115 78 37 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 251 212 65 23 pint:PI1077|3end_hypothetical_protein_999569..999719_POSITIVE 281 158 115 120 79 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 261 305 262 51 11 pint:PI1766|3end_sugar_transporter,_possible_glucose/galactose_transporter_1694350..1694500_POSITIVE 258 158 115 61 20 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 254 245 216 127 100 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 252 249 209 143 102 pint:PI0019|3end_conserved_hypothetical_protein_24454..24604_POSITIVE 250 305 263 142 108 pint:PI0114|3end_hypothetical_protein_108527..108677_POSITIVE 249 246 211 124 84 pint:PI0265|3end_conjugative_transposon_protein,_TraO_257957..258107_POSITIVE 245 158 116 120 75 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 245 158 116 61 16 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 244 239 213 145 112 pint:PI0958|3end_G:T/U_mismatch-specific_DNA_glycosylase_890569..890719_POSITIVE 242 150 102 151 102 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 242 150 102 151 102 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 242 254 211 42 2 pint:PI0286|3end_ISPg3-related_transposase_272065..272215_POSITIVE 242 250 203 125 69 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 240 252 221 148 121 pint:PI1633|3end_hypothetical_protein_1552955..1553105_POSITIVE 239 139 101 118 76 pint:PI0060|3end_long-chain-fatty-acid--CoA_ligase_(acyl-CoA_synthetase)_60166..60316_POSITIVE 232 305 274 122 92 pint:PI1715|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1632324..1632474_POSITIVE 231 249 209 139 95 pint:PI0351|3end_hypothetical_protein_324768..324918_POSITIVE 226 325 288 68 28 pint:PI1103|3end_hypothetical_protein_1027175..1027325_POSITIVE