Origin IGS: taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaagatatataaatgatggttgggagcttcggcttccaactgacttttaatctttaacatttacttatttcttacttaggtttcgttcattcagactctaagacaagaaaaaggagttaaggagttaagccaatcgttttacttacgtttcataagtgtatagtagaactctttgcttaactccttaattctatatctgaaagaaacctaagagcgtctcgtaatcacgatgacagaatttaaatcagcaaatta Mask Tandem Repeat Region ================================================ taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Find is-nt database================================================ Query_seq: PI2149:PI2150|PI2149:PI2150:3-oxoacyl-acyl-carrier-protein synthase:acyl carrier protein:->->:2096854..2097111 258 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2149:PI2150|PI2149:PI2150:3-oxoacyl-acyl-carrier-protein synthase:acyl carrier protein:->->:2096854..2097111 258 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2149:PI2150|PI2149:PI2150:3-oxoacyl-acyl-carrier-protein synthase:acyl carrier protein:->->:2096854..2097111 258 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Predict ORF larger than 30AA ================================================ taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2149:PI2150|PI2149:PI2150:3-oxoacyl-acyl-carrier-protein synthase:acyl carrier protein:->->:2096854..2097111 258 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Intra-Species Hit: Count: 1 Min: 1 Max: 258 Len: 258 Subject: pint_PI2149_PI2150|3-oxoacyl-acyl-carrier-protein synthase:acyl carrier protein|POSITIVE:POSITIVE|[2096854,2097111]|258 HSP 1 e-value: 1.0E-144 bit: 511.0 Len: 258 Query Start:1 Query End:258 Subject Strand: POSITIVE Subject Start: 1 Subject End: 258 taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta taatttgctgatttaaattctgtcatcgtgattacgagacgctcttaggtttctttcagatatagaattaaggagttaagcaaagagttctactatacacttatgaaacgtaagtaaaacgattggcttaactccttaactcctttttcttgtcttagagtctgaatgaacgaaacctaagtaagaaataagtaaatgttaaagattaaaagtcagttggaagccgaagctcccaaccatcatttatatatcttctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUGCUGAUUUAAAUUCUGUCAUCGUGAUUACGAGACGCUCUUAGGUUUCUUUCAGAUAUAGAAUUAAGGAGUUAAGCAAAGAGUUCUACUAUACACUUAUGAAACGUAAGUAAAACGAUUGGCUUAACUCCUUAACUCCUUUUUCUUGUCUUAGAGUCUGAAUGAACGAAACCUAAGUAAGAAAUAAGUAAAUGUUAAAGAUUAAAAGUCAGUUGGAAGCCGAAGCUCCCAACCAUCAUUUAUAUAUCUUCUUA .....................(((.((((....)))))))...((((((((((.(((((((...((.((((((((((((((.((..(((.((((.(((...........))))))).)))..)).)))))))))))))).)).....(((......)))))))))).....)))))))))).((((((((.(((((((.....(((.....))).(((((.(((....))).)))))...))))))).))).))))). (-71.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAGAUAUAUAAAUGAUGGUUGGGAGCUUCGGCUUCCAACUGACUUUUAAUCUUUAACAUUUACUUAUUUCUUACUUAGGUUUCGUUCAUUCAGACUCUAAGACAAGAAAAAGGAGUUAAGGAGUUAAGCCAAUCGUUUUACUUACGUUUCAUAAGUGUAUAGUAGAACUCUUUGCUUAACUCCUUAAUUCUAUAUCUGAAAGAAACCUAAGAGCGUCUCGUAAUCACGAUGACAGAAUUUAAAUCAGCAAAUUA (((((.((((..((((((..((((((((((....))))))))))((.......)).....))))))..)))))))))((((((((((.....((((((.(((......)))....((((((((((((((((((.((..((((((((((((.........)))).))))))))..)).))))))))))))))))))...)))))).))))))))))...(((((((....)))).)))..................... (-85.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 139 109 205 178 pint:PI1026|5end_uridine_phosphorylase_942300..942530_POSITIVE 280 160 124 225 179 pint:PI0977|5end_riboflavin_synthase,_alpha_subunit_904043..904273_POSITIVE 280 245 212 67 35 pint:PI0888|5end_hypothetical_protein_817751..817981_POSITIVE 262 240 212 124 94 pint:PI0989|5end_mannose-1-phosphate_guanylyltransferase_912833..913063_POSITIVE 261 69 24 51 2 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 254 176 140 135 99 pint:PI0268|5end_lysozyme-related_protein_259198..259428_POSITIVE 244 158 123 149 116 pint:PI1668|5end_conserved_hypothetical_protein_1580692..1580922_POSITIVE 244 240 215 169 141 pint:PI0676|5end_hypothetical_protein_614576..614806_POSITIVE 244 60 19 203 148 pint:PI0449|5end_hypothetical_protein_422952..423182_POSITIVE 242 49 20 122 85 pint:PI0715|5end_hypothetical_protein_652311..652541_POSITIVE 241 54 18 199 154 pint:PI0226|5end_ABC_transporter,_ATP-binding_/_permease_protein_222110..222340_POSITIVE 241 54 18 127 82 pint:PI0225|5end_conserved_hypothetical_protein_222038..222268_POSITIVE 240 229 212 75 57 pint:PI0273|5end_hypothetical_protein_262956..263186_POSITIVE 239 248 202 83 20 pint:PI0772|5end_conserved_hypothetical_protein_707157..707387_POSITIVE 238 231 207 33 4 pint:PI1059|5end_conserved_hypothetical_protein_978114..978344_POSITIVE 234 216 179 42 4 pint:PI1027|5end_hypothetical_protein_943197..943427_POSITIVE 234 127 84 80 36 pint:PI0189|5end_integrase_179623..179853_POSITIVE 230 162 122 81 29 pint:PI1637|5end_hypothetical_protein_1554500..1554730_POSITIVE 230 55 17 69 28 pint:PI1078|5end_conserved_hypothetical_protein_999500..999730_POSITIVE 227 80 31 225 168 pint:PI1721|5end_peptidyl-prolyl_cis-trans_isomerase,_PPIC-type_1638086..1638316_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 139 109 57 30 pint:PI1025|3end_tRNA_modification_GTPase_942232..942382_POSITIVE 280 186 149 86 51 pint:PI1375|3end_protease_IV_family,_clan_S_(peptidase_family_S49)_1297614..1297764_POSITIVE 280 245 212 45 13 pint:PI0886|3end_hypothetical_protein_817809..817959_POSITIVE 261 69 24 60 11 pint:PI0736|3end_transcriptional_regulator,_TetR_family_671271..671421_POSITIVE 254 176 140 148 112 pint:PI0267|3end_conjugative_transposon_protein,_TraQ_259291..259441_POSITIVE 252 75 31 73 24 pint:PI2083|3end_hypothetical_protein_2040661..2040811_POSITIVE 244 158 123 149 116 pint:PI1667|3end_conserved_hypothetical_protein_1580772..1580922_POSITIVE 239 139 92 139 87 pint:PI0800|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_739331..739481_POSITIVE 237 180 131 46 3 pint:PI2149|3end_3-oxoacyl-acyl-carrier-protein_synthase_2096793..2096943_POSITIVE 236 163 124 117 81 pint:PI1653|3end_hypothetical_protein_1565714..1565864_POSITIVE 234 127 84 47 3 pint:PI0188|3end_phosphomethylpyrimidine_kinase_179670..179820_POSITIVE 230 55 17 58 17 pint:PI1077|3end_hypothetical_protein_999569..999719_POSITIVE 228 179 136 119 74 pint:PI0945|3end_sodium:solute_symporter_family_protein_877624..877774_POSITIVE 227 235 211 53 27 pint:PI0334|3end_hypothetical_protein_313598..313748_POSITIVE 226 175 135 80 42 pint:PI0282|3end_hypothetical_protein_266826..266976_POSITIVE 224 134 92 77 38 pint:PI0073|3end_dihydropteroate_synthase_71785..71935_POSITIVE 223 172 132 121 84 pint:PI1622|3end_hypothetical_protein_1547230..1547380_POSITIVE 222 56 17 89 49 pint:PI1807|3end_hypothetical_protein_1742481..1742631_POSITIVE 220 241 201 47 10 pint:PI1008|3end_cytochrome_c_nitrite_reductase,_small_subunit_929331..929481_POSITIVE 219 55 18 89 48 pint:PI1494|3end_conserved_hypothetical_protein_1413968..1414118_POSITIVE