Origin IGS: atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgagaatgattacgatttgcgagtcaaacccataaaattcgttcgttggagaaggtttttcaactagtatttctagtattcctaagatttttagaaatatttaaatgaatatatgtaaaatcgctttgattttgtaaaaataattccaacgaaaaacggtaatttcgat Mask Tandem Repeat Region ================================================ atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Find is-nt database================================================ Query_seq: PI2131:PI2132|PI2131:PI2132:hypothetical protein:hypothetical protein:->->:2080929..2081100 172 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2131:PI2132|PI2131:PI2132:hypothetical protein:hypothetical protein:->->:2080929..2081100 172 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2131:PI2132|PI2131:PI2132:hypothetical protein:hypothetical protein:->->:2080929..2081100 172 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 70 End: 171 ..................................................................... M F L K I L G I L E I L V E K P S P T N E F Y G F D S Q I V I I L I . atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2131:PI2132|PI2131:PI2132:hypothetical protein:hypothetical protein:->->:2080929..2081100 172 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Intra-Species Hit: Count: 1 Min: 1 Max: 172 Len: 172 Subject: pint_PI2131_PI2132|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2080929,2081100]|172 HSP 1 e-value: 5.0E-93 bit: 341.0 Len: 172 Query Start:1 Query End:172 Subject Strand: POSITIVE Subject Start: 1 Subject End: 172 atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta atcgaaattaccgtttttcgttggaattatttttacaaaatcaaagcgattttacatatattcatttaaatatttctaaaaatcttaggaatactagaaatactagttgaaaaaccttctccaacgaacgaattttatgggtttgactcgcaaatcgtaatcattctcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCGAAAUUACCGUUUUUCGUUGGAAUUAUUUUUACAAAAUCAAAGCGAUUUUACAUAUAUUCAUUUAAAUAUUUCUAAAAAUCUUAGGAAUACUAGAAAUACUAGUUGAAAAACCUUCUCCAACGAACGAAUUUUAUGGGUUUGACUCGCAAAUCGUAAUCAUUCUCAUUA ...((((((..(((...((((((((..((((((((.((((((.....)))))).................(((((((((.....))))))))).))))))))...(((....)))....))))))))))))))))).((((..(((..((.....))...)))..))))... (-31.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAGAAUGAUUACGAUUUGCGAGUCAAACCCAUAAAAUUCGUUCGUUGGAGAAGGUUUUUCAACUAGUAUUUCUAGUAUUCCUAAGAUUUUUAGAAAUAUUUAAAUGAAUAUAUGUAAAAUCGCUUUGAUUUUGUAAAAAUAAUUCCAACGAAAAACGGUAAUUUCGAU ...(((((.(((((.((.....)))))))............(((((((((((((.......((((...(((((((((((...((....))...)))))))))))....))))......(((((((.....))))))).........)))))))))...))))...))))).. (-34.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 562 50 3 181 134 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 470 49 1 80 32 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 426 48 1 118 71 pint:PI1786|5end_hypothetical_protein_1718124..1718354_POSITIVE 411 46 1 96 52 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 387 47 1 230 184 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 317 50 1 119 70 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 305 54 12 87 45 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 292 48 1 88 41 pint:PI2141|5end_hypothetical_protein_2087169..2087399_POSITIVE 291 38 1 129 92 pint:PI0926|5end_hypothetical_protein_856824..857054_POSITIVE 287 48 1 139 92 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 275 47 1 138 92 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 261 55 16 41 1 pint:PI0069|5end_hypothetical_protein_67072..67302_POSITIVE 258 151 114 67 27 pint:PI1419|5end_conserved_hypothetical_protein;_membrane_protease_subunits,_stomatin/prohibitin_homologs_1339136..1339366_POSITIVE 256 157 116 178 138 pint:PI1704|5end_50S_ribosomal_protein_L32_1621418..1621648_POSITIVE 242 170 121 84 43 pint:PI0764|5end_permease_697962..698192_POSITIVE 237 158 113 94 53 pint:PI1496|5end_nucleoside_phosphorylase_1417116..1417346_POSITIVE 234 40 16 25 1 pint:PI1521|5end_hypothetical_protein_1441220..1441450_POSITIVE 233 167 125 177 132 pint:PI0313|5end_conserved_hypothetical_protein_294833..295063_POSITIVE 233 167 125 65 20 pint:PI0311|5end_hypothetical_protein_294721..294951_POSITIVE 222 151 114 64 26 pint:PI0479|5end_50S_ribosomal_protein_L15_441125..441355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 410 40 1 95 56 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 391 47 1 87 42 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 258 151 114 52 12 pint:PI1418|3end_UDP-N-acetylglucosamine_2-epimerase_1339201..1339351_POSITIVE 242 170 121 44 3 pint:PI0763|3end_thymidine_kinase_698002..698152_POSITIVE 241 49 12 40 4 pint:PI1379|3end_hypothetical_protein_1302292..1302442_POSITIVE 236 150 116 148 120 pint:PI1703|3end_conserved_hypothetical_protein_1621480..1621630_POSITIVE 210 150 106 104 57 pint:PI0582|3end_hypothetical_protein_525214..525364_POSITIVE 210 167 124 89 50 pint:PI0238|3end_two-component_system_response_regulator_237826..237976_POSITIVE 207 44 1 130 87 pint:PI0138|3end_hypothetical_protein_131365..131515_POSITIVE 206 169 134 79 41 pint:PI0483|3end_30S_ribosomal_protein_S13_444015..444165_POSITIVE 203 168 132 141 90 pint:PI0264|3end_transfer_region-related_protein,_TraN_257371..257521_POSITIVE 201 151 121 35 4 pint:PI0478|3end_50S_ribosomal_protein_L30_441176..441326_POSITIVE 201 157 115 88 38 pint:PI0067|3end_hypothetical_protein_66586..66736_POSITIVE 200 48 1 53 5 pint:PI1882|3end_hypothetical_protein_1826487..1826637_POSITIVE 198 157 117 131 95 pint:PI0392|3end_possible_transcriptional_regulator_358735..358885_POSITIVE 196 49 12 75 34 pint:PI0629|3end_heat_shock_protein_15_577122..577272_POSITIVE 194 166 124 59 14 pint:PI1972|3end_hypothetical_protein_1930503..1930653_POSITIVE 191 50 8 92 51 pint:PI2042|3end_cation-transporting_ATPase_1996138..1996288_POSITIVE 191 152 112 125 81 pint:PI0315|3end_conserved_hypothetical_protein_298186..298336_POSITIVE 190 48 3 56 14 pint:PI2021|3end_DNA_repair_protein_1978631..1978781_POSITIVE