Origin IGS: taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaacggtcttcaatacgttataaacggcgagccgctgttcccacatagtgcaggaacagcggctcatttcgtatttgtaatgatggaagagtggggctttcgtta Mask Tandem Repeat Region ================================================ taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Find is-nt database================================================ Query_seq: PI2096:PI2097|PI2096:PI2097:conserved hypothetical protein; possible phosphoesterase:conserved hypothetical protein:->->:2050945..2051051 107 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2096:PI2097|PI2096:PI2097:conserved hypothetical protein; possible phosphoesterase:conserved hypothetical protein:->->:2050945..2051051 107 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2096:PI2097|PI2096:PI2097:conserved hypothetical protein; possible phosphoesterase:conserved hypothetical protein:->->:2050945..2051051 107 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Predict ORF larger than 30AA ================================================ taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 93 HP_score: -12.1 Tail_Score: -3.92602 Start: 37 End: 81 Full_Region: ATCATTACAAATACG AAATGAGCCGCTGTTCCTGCA CTA TGTGGGAACAGCGGCTCGCCG TTTATAACGTATTGA ....................................AAATGAGCCGCTGTTCCTGCACTATGTGGGAACAGCGGCTCGCCG.......................... TransTerm Strand: + Conf: 95 HP_score: -15.6 Tail_Score: -2.95079 Start: 38 End: 80 Full_Region: TCATTACAAATACGA AATGAGCCGCTGTTCCTGCA CTA TGTGGGAACAGCGGCTCGCC GTTTATAACGTATTG .....................................AATGAGCCGCTGTTCCTGCACTATGTGGGAACAGCGGCTCGCC........................... TransTerm Strand: - Conf: 100 HP_score: -16.0 Tail_Score: -4.05181 Start: 40 End: 78 Full_Region: atacgttataaacgg cgagccgctgttcccaca tag tgcaggaacagcggctca tttcgtatttgtaat .......................................cgagccgctgttcccacatagtgcaggaacagcggctca............................. TransTerm Strand: - Conf: 100 HP_score: -19.5 Tail_Score: -3.02024 Start: 41 End: 77 Full_Region: tacgttataaacggc gagccgctgttcccaca tag tgcaggaacagcggctc atttcgtatttgtaa ........................................gagccgctgttcccacatagtgcaggaacagcggctc.............................. TransTerm Strand: - Conf: 100 HP_score: -16.3 Tail_Score: -3.05358 Start: 43 End: 75 Full_Region: cgttataaacggcga gccgctgttcccaca tag tgcaggaacagcggc tcatttcgtatttgt ..........................................gccgctgttcccacatagtgcaggaacagcggc................................ Find igs database================================================ Query_seq: PI2096:PI2097|PI2096:PI2097:conserved hypothetical protein; possible phosphoesterase:conserved hypothetical protein:->->:2050945..2051051 107 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Intra-Species Hit: Count: 1 Min: 1 Max: 107 Len: 107 Subject: pint_PI2096_PI2097|conserved hypothetical protein; possible phosphoesterase:conserved hypothetical protein|POSITIVE:POSITIVE|[2050945,2051051]|107 HSP 1 e-value: 2.0E-54 bit: 212.0 Len: 107 Query Start:1 Query End:107 Subject Strand: POSITIVE Subject Start: 1 Subject End: 107 taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta taacgaaagccccactcttccatcattacaaatacgaaatgagccgctgttcctgcactatgtgggaacagcggctcgccgtttataacgtattgaagaccgtttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAAAGCCCCACUCUUCCAUCAUUACAAAUACGAAAUGAGCCGCUGUUCCUGCACUAUGUGGGAACAGCGGCUCGCCGUUUAUAACGUAUUGAAGACCGUUUUA .....(((((.....(((((.............(((...((((((((((((((..(.....)..)))))))))))))).)))............)))))..))))). (-38.11) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACGGUCUUCAAUACGUUAUAAACGGCGAGCCGCUGUUCCCACAUAGUGCAGGAACAGCGGCUCAUUUCGUAUUUGUAAUGAUGGAAGAGUGGGGCUUUCGUUA .....((.(((((.((.((((((((((((.((((((((((((((((...)))..)))))))))))))...))))..)))))))))).))))).))............ (-43.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 362 82 49 66 20 pint:PI0300|5end_transposase_285163..285393_POSITIVE 357 90 41 213 167 pint:PI1811|5end_conserved_hypothetical_protein_1749518..1749748_POSITIVE 349 84 41 136 101 pint:PI0938|5end_hypothetical_protein_869345..869575_POSITIVE 349 82 45 75 23 pint:PI0314|5end_conserved_hypothetical_protein_295552..295782_POSITIVE 340 79 35 89 50 pint:PI1760|5end_hypothetical_protein_1684413..1684643_POSITIVE 335 81 43 53 5 pint:PI0729|5end_integrase_661428..661658_POSITIVE 335 74 35 66 12 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 334 87 43 223 172 pint:PI1635|5end_hypothetical_protein_1553948..1554178_POSITIVE 333 90 44 170 123 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 330 78 34 89 36 pint:PI0672|5end_conserved_hypothetical_protein_611746..611976_POSITIVE 328 99 53 69 7 pint:PI0588|5end_hypothetical_protein_535016..535246_POSITIVE 327 79 34 52 1 pint:PI1564|5end_surface_antigen_BspA_1483455..1483685_POSITIVE 327 75 35 220 187 pint:PI0956|5end_conserved_hypothetical_protein_886077..886307_POSITIVE 325 50 9 177 137 pint:PI0338|5end_hypothetical_protein_315337..315567_POSITIVE 324 80 35 47 2 pint:PI0814|5end_conserved_hypothetical_protein_752946..753176_POSITIVE 319 81 41 162 120 pint:PI2133|5end_collagenase_2081106..2081336_POSITIVE 316 90 42 218 158 pint:PI1675|5end_TonB-dependent_outer_membrane_receptor_1588107..1588337_POSITIVE 316 84 41 160 119 pint:PI0261|5end_conjugative_transposon_protein,_TraK_254065..254295_POSITIVE 313 90 41 227 169 pint:PI0404|5end_integrase_371295..371525_POSITIVE 312 77 35 122 89 pint:PI1948|5end_hypothetical_protein_1903084..1903314_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 587 77 34 50 9 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 372 90 43 125 63 pint:PI1623|3end_hypothetical_protein_1549261..1549411_POSITIVE 372 90 43 125 63 pint:PI0330|3end_hypothetical_protein_312545..312695_POSITIVE 358 90 42 117 61 pint:PI0857|3end_hypothetical_protein_790986..791136_POSITIVE 352 81 43 146 93 pint:PI0331|3end_hypothetical_protein_312575..312725_POSITIVE 340 79 35 139 100 pint:PI1761|3end_hypothetical_protein_1684543..1684693_POSITIVE 335 81 43 53 5 pint:PI0728|3end_integrase_661508..661658_POSITIVE 333 90 44 150 103 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 330 78 34 70 17 pint:PI0671|3end_hypothetical_protein_611807..611957_POSITIVE 327 75 35 147 114 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 319 81 41 115 73 pint:PI2132|3end_hypothetical_protein_2081139..2081289_POSITIVE 316 84 41 137 96 pint:PI0260|3end_conjugative_transposon_protein,_TraJ_254122..254272_POSITIVE 315 78 38 59 20 pint:PI1708|3end_hypothetical_protein_1625562..1625712_POSITIVE 315 82 44 86 50 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 312 74 39 38 1 pint:PI0262|3end_conserved_hypothetical_protein_255094..255244_POSITIVE 311 80 34 68 15 pint:PI0091|3end_hypothetical_protein_89774..89924_POSITIVE 306 90 44 115 62 pint:PI1794|3end_conserved_hypothetical_protein_1726696..1726846_POSITIVE 301 76 36 129 88 pint:PI1577|3end_conserved_hypothetical_protein_1503879..1504029_POSITIVE 300 79 34 148 95 pint:PI0276|3end_anti-restriction_protein_265021..265171_POSITIVE 298 80 33 151 105 pint:PI2045|3end_hypothetical_protein_1998723..1998873_POSITIVE