Origin IGS: tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcttaacgtgttctctgccagtttctacgtatctcccacttgtttcctacatatttcccactttttttaagcgtattctaagcttgtttcttacgcattgttgcttggtattatagcgaacattacactactttctcta Mask Tandem Repeat Region ================================================ tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggcagagaacacgttaagaa Find is-nt database================================================ Query_seq: PI2082:PI2083|PI2082:PI2083:hypothetical protein:hypothetical protein:->->:2040324..2040463 140 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2082:PI2083|PI2082:PI2083:hypothetical protein:hypothetical protein:->->:2040324..2040463 140 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2082:PI2083|PI2082:PI2083:hypothetical protein:hypothetical protein:->->:2040324..2040463 140 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 27 End: 125 .......................... L V L C C H T L F L S L I R K F F T P F I Y S V L P L Y T S V P M ............... tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2082:PI2083|PI2082:PI2083:hypothetical protein:hypothetical protein:->->:2040324..2040463 140 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Intra-Species Hit: Count: 1 Min: 1 Max: 140 Len: 140 Subject: pint_PI2082_PI2083|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2040324,2040463]|140 HSP 1 e-value: 2.0E-61 bit: 236.0 Len: 140 Query Start:1 Query End:140 Subject Strand: POSITIVE Subject Start: 1 Subject End: 140 tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttnnnnnnngtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa tagagaaagtagtgtaatgttcgctataataccaagcaacaatgcgtaagaaacaagcttagaatacgcttaaaaaaagtgggaaatatgtaggaaacaagtgggagatacgtagaaactggcagagaacacgttaagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAGAAAGUAGUGUAAUGUUCGCUAUAAUACCAAGCAACAAUGCGUAAGAAACAAGCUUAGAAUACGCUUAAAAAAAGUGGGAAAUAUGUAGGAAACAAGUGGGAGAUACGUAGAAACUGGCAGAGAACACGUUAAGAA ...........((((..((((.(((.........))))))).((((((........((.((.....(((((.....))))).....)).)).(....)..........))))))..............))))........ (-19.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUAACGUGUUCUCUGCCAGUUUCUACGUAUCUCCCACUUGUUUCCUACAUAUUUCCCACUUUUUUUAAGCGUAUUCUAAGCUUGUUUCUUACGCAUUGUUGCUUGGUAUUAUAGCGAACAUUACACUACUUUCUCUA ........((((((.((((((((..(..((((.........(((.....)))................(((((.........))))).....))))....)..)).)))).....)).))))))................ (-17.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 320 60 11 87 30 pint:PI0992|5end_RNA_polymerase_sigma-70_factor_916456..916686_POSITIVE 294 47 1 125 91 pint:PI1083|5end_TPR-repeat-containing_protein_1001975..1002205_POSITIVE 284 140 93 79 29 pint:PI1138|5end_hypothetical_protein_1064560..1064790_POSITIVE 282 136 93 159 118 pint:PI0349|5end_hypothetical_protein_323144..323374_POSITIVE 280 135 98 115 83 pint:PI0168|5end_methionyl-tRNA_synthetase_(methionine--tRNA_ligase)_161181..161411_POSITIVE 267 132 93 154 118 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 265 134 92 133 82 pint:PI1456|5end_conserved_hypothetical_protein_1378864..1379094_POSITIVE 263 126 81 157 117 pint:PI1886|5end_cell_division_protein_1827633..1827863_POSITIVE 261 140 104 162 122 pint:PI1856|5end_nicotinate-nucleotide_adenylyltransferase_1803203..1803433_POSITIVE 260 128 92 229 184 pint:PI0900|5end_signal_recognition_particle_protein_829663..829893_POSITIVE 259 140 97 97 39 pint:PI2040|5end_conserved_hypothetical_protein;_possible_transporter_1993068..1993298_POSITIVE 256 129 81 46 2 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 255 86 43 61 14 pint:PI1696|5end_conserved_hypothetical_protein_1610544..1610774_POSITIVE 248 111 76 52 21 pint:PI1968|5end_hypothetical_protein_1924378..1924608_POSITIVE 248 106 67 81 42 pint:PI1050|5end_conserved_hypothetical_protein_967246..967476_POSITIVE 248 140 102 78 44 pint:PI0088|5end_S-adenosylmethionine_synthetase_86663..86893_POSITIVE 247 130 81 77 23 pint:PI0805|5end_conserved_hypothetical_protein_742729..742959_POSITIVE 246 128 100 168 144 pint:PI1857|5end_guanylate_kinase_1803816..1804046_POSITIVE 246 132 93 71 20 pint:PI0723|5end_conserved_hypothetical_protein_656043..656273_POSITIVE 242 121 81 47 12 pint:PI1066|5end_dihydrofolate_reductase_983427..983657_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 140 93 57 7 pint:PI1137|3end_hypothetical_protein_1064618..1064768_POSITIVE 282 136 93 146 105 pint:PI0348|3end_conserved_hypothetical_protein_323211..323361_POSITIVE 280 135 98 112 80 pint:PI0166|3end_hypothetical_protein_161258..161408_POSITIVE 267 132 93 141 105 pint:PI1642|3end_conserved_hypothetical_protein_1560439..1560589_POSITIVE 263 126 81 83 43 pint:PI1885|3end_cell_division_protein_1827639..1827789_POSITIVE 262 130 81 82 26 pint:PI1954|3end_translation_initiation_factor_IF-2_1911647..1911797_POSITIVE 261 140 104 47 7 pint:PI1855|3end_lipopolysaccharide_biosynthesis_protein_1803168..1803318_POSITIVE 261 118 78 44 1 pint:PI1613|3end_hypothetical_protein_1540906..1541056_POSITIVE 249 133 92 151 101 pint:PI1455|3end_hypothetical_protein_1378963..1379113_POSITIVE 248 111 76 35 4 pint:PI1967|3end_hypothetical_protein_1924441..1924591_POSITIVE 248 106 67 96 57 pint:PI1049|3end_hypothetical_protein_967341..967491_POSITIVE 248 140 102 68 34 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 247 130 81 58 4 pint:PI0804|3end_conserved_hypothetical_protein;_possible_membrane_protein_742790..742940_POSITIVE 243 136 103 131 79 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 238 140 120 122 99 pint:PI1587|3end_conserved_hypothetical_protein;_possible_permease_1516266..1516416_POSITIVE 237 51 13 119 87 pint:PI1343|3end_conserved_hypothetical_protein_1258539..1258689_POSITIVE 237 133 91 129 95 pint:PI0374|3end_MbpB_protein_345727..345877_POSITIVE 235 69 23 148 93 pint:PI0919|3end_conserved_hypothetical_protein_849719..849869_POSITIVE 235 84 41 96 35 pint:PI0015|3end_possible__transport_protein_20150..20300_POSITIVE 234 128 81 73 15 pint:PI0883|3end_para-aminobenzoate_synthase_component_I_817251..817401_POSITIVE