Origin IGS: taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctttgctcccaataccagtttataaaatctttataactggttttggaccatgcgccatgtcaaatccatttcctatgatgacaagtctgttcattcttcgttatcgttatta Mask Tandem Repeat Region ================================================ taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Find is-nt database================================================ Query_seq: PI2066:PI2067|PI2066:PI2067:hypothetical protein:hypothetical protein:->->:2027787..2027899 113 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2066:PI2067|PI2066:PI2067:hypothetical protein:hypothetical protein:->->:2027787..2027899 113 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2066:PI2067|PI2066:PI2067:hypothetical protein:hypothetical protein:->->:2027787..2027899 113 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 3 End: 110 .. Y R Y R L I F L S T M M P F P N S M A C P G F G T I F I K Y V P I P L M ... taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI2066:PI2067|PI2066:PI2067:hypothetical protein:hypothetical protein:->->:2027787..2027899 113 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Intra-Species Hit: Count: 1 Min: 1 Max: 113 Len: 113 Subject: pint_PI2066_PI2067|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2027787,2027899]|113 HSP 1 e-value: 5.0E-58 bit: 224.0 Len: 113 Query Start:1 Query End:113 Subject Strand: POSITIVE Subject Start: 1 Subject End: 113 taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga taataacgataacgaagaatgaacagacttgtcatcataggaaatggatttgacatggcgcatggtccaaaaccagttataaagattttataaactggtattgggagcaaaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACGAUAACGAAGAAUGAACAGACUUGUCAUCAUAGGAAAUGGAUUUGACAUGGCGCAUGGUCCAAAACCAGUUAUAAAGAUUUUAUAAACUGGUAUUGGGAGCAAAGA ...........................(.(((((((((.....))))...))))).)..((....(((((.(((((((((((.....)))).))))))).))))).))..... (-21.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUUUGCUCCCAAUACCAGUUUAUAAAAUCUUUAUAACUGGUUUUGGACCAUGCGCCAUGUCAAAUCCAUUUCCUAUGAUGACAAGUCUGUUCAUUCUUCGUUAUCGUUAUUA .....((..((((.(((((((.((((.....))))))))))).)))).....)).....................(((((((((((..........))).))))))))..... (-22.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 286 68 29 176 142 pint:PI1517|5end_AcrB/AcrD_family_multidrug_resistance_protein_1435831..1436061_POSITIVE 281 67 21 116 72 pint:PI1650|5end_N-acetylmuramoyl-L-alanine_amidase_1563195..1563425_POSITIVE 266 63 24 68 18 pint:PI0903|5end_conserved_hypothetical_protein_832674..832904_POSITIVE 264 65 28 93 54 pint:PI0263|5end_conjugative_transposon_protein,_TraM_255256..255486_POSITIVE 261 76 40 213 170 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 254 68 31 104 62 pint:PI0384|5end_probable_radical_SAM_domain_protein_352884..353114_POSITIVE 247 63 28 196 161 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 244 77 31 83 32 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 244 77 31 83 32 pint:PI0349|5end_hypothetical_protein_323144..323374_POSITIVE 244 75 31 121 57 pint:PI0273|5end_hypothetical_protein_262956..263186_POSITIVE 243 64 21 105 63 pint:PI0782|5end_beta-lactamase_induction_signal_transducer,_AmpG_protein_720573..720803_POSITIVE 241 69 22 187 141 pint:PI0754|5end_possible_outer_membrane_receptor_683771..684001_POSITIVE 240 60 13 231 193 pint:PI0732|5end_conserved_hypothetical_protein_663598..663828_POSITIVE 239 66 24 196 136 pint:PI0769|5end_phosphomannomutase_704653..704883_POSITIVE 238 65 21 82 31 pint:PI1545|5end_conserved_hypothetical_protein_1463587..1463817_POSITIVE 237 77 37 72 32 pint:PI1802|5end_50S_ribosomal_protein_L13_1734350..1734580_POSITIVE 235 42 7 163 133 pint:PI1728|5end_ATP-dependent_Clp_protease,_proteolytic_subunit_1646916..1647146_POSITIVE 235 78 31 142 84 pint:PI0729|5end_integrase_661428..661658_POSITIVE 233 90 47 75 21 pint:PI0839|5end_conserved_hypothetical_protein_773843..774073_POSITIVE 233 60 20 184 150 pint:PI0316|5end_conserved_hypothetical_protein_298379..298609_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 47 7 41 12 pint:PI1065|3end_hypothetical_protein_983650..983800_POSITIVE 260 68 21 54 12 pint:PI0057|3end_conserved_hypothetical_protein_56512..56662_POSITIVE 256 60 11 84 31 pint:PI0244|3end_possible_mobilization_protein_C_242799..242949_POSITIVE 244 77 31 70 19 pint:PI1642|3end_conserved_hypothetical_protein_1560439..1560589_POSITIVE 244 77 31 70 19 pint:PI0348|3end_conserved_hypothetical_protein_323211..323361_POSITIVE 239 66 24 124 64 pint:PI0768|3end_conserved_hypothetical_protein;_possible_acetyltransferase_704661..704811_POSITIVE 239 60 12 151 110 pint:PI0224|3end_conserved_hypothetical_protein_221979..222129_POSITIVE 238 65 21 94 43 pint:PI1544|3end_hypothetical_protein_1463679..1463829_POSITIVE 238 59 11 95 41 pint:PI0892|3end_acyl-ACP_thioesterase_823939..824089_POSITIVE 237 77 37 54 14 pint:PI1801|3end_30S_ribosomal_protein_S9_1734412..1734562_POSITIVE 235 78 31 142 84 pint:PI0728|3end_integrase_661508..661658_POSITIVE 233 60 11 136 94 pint:PI1889|3end_UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape_ptide)_pyrophosphoryl-undecaprenol_N-acetylglucosamine_transferase_1832591..1832741_POSITIVE 233 90 47 79 25 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 232 66 21 87 37 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 232 65 30 130 82 pint:PI0374|3end_MbpB_protein_345727..345877_POSITIVE 230 69 21 124 73 pint:PI0896|3end_2-oxoglutarate_oxidoreductase,_alpha_subunit_828276..828426_POSITIVE 228 65 21 60 16 pint:PI0993|3end_probable_TPR-repeat-containing_protein_918481..918631_POSITIVE 226 65 22 130 83 pint:PI1542|3end_mobilization_protein_1462866..1463016_POSITIVE 226 70 21 79 24 pint:PI0221|3end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_217796..217946_POSITIVE 224 69 22 134 82 pint:PI2098|3end_hypothetical_protein_2052473..2052623_POSITIVE