Origin IGS: tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcttctatctctatgggaagtttgacactcaaatcccacagggctttgttcagctgagagggcgatggtgcccagaaaggagaggctttgccgtctttaccgaactttgttaagttgccagagtgcatcaaagccactttcaaattaaactttgtgcctggctgtaagaaacgtatgaaaccgaaaaagcccatagggttgctttcgccgatgaactttcctttgtatgaatcggcgtaagtgtattcgtaaggcagctttgtgcggtctcttaccaatacattggccgtgccgttgtttacataatacataaagaaatgctggtgaatcttgtcttgttcgttatcgtagaattggtgattcatgtgctctccttttgcattgtagtagtccatacgcaaggaatagaccacatcggggtgcttcttcgtgttttttacaacgaatttggtctgtcctccgtcgcctgtcttgaagttttcgcctttcacaacttcccaaacaattttctgaacattgcctgtcggcacgaaatctgcctcttgtggcatattctcgaatgctgctccgctgcgcagtttgccctctgtgagtgtgaaaacagactgtgtagggtcttcgtgcaacttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaataacgtgagttcaatgaatttattggttgtttcatcgtgtgctaaaccgctttcacaccctacaagtaaggtgcgtttatataccactcgtgatatacacttgcaccccactcgtggtatatgctcgtaccccactcgtggtatacgtttgtatatcacgagtaggttacagcaacaataaatttacaaacaattacagttcgtcgaactcacattaaataaagacataagtataataagtaaacaataaaaagtaaaaagatgaagattaaaaacagtatttttcctattttattaggtatgctcagtattggtttcttctctgcttgcaatcctgaaaagccagaagacgagaaggaaaacaagttgcacgaagaccctacacagtctgttttcacactcacagagggcaaactgcgcagcggagcagcattcgagaatatgccacaagaggcagatttcgtgccgacaggcaatgttcagaaaattgtttgggaagttgtgaaaggcgaaaacttcaagacaggcgacggaggacagaccaaattcgttgtaaaaaacacgaagaagcaccccgatgtggtctattccttgcgtatggactactacaatgcaaaaggagagcacatgaatcaccaattctacgataacgaacaagacaagattcaccagcatttctttatgtattatgtaaacaacggcacggccaatgtattggtaagagaccgcacaaagctgccttacgaatacacttacgccgattcatacaaaggaaagttcatcggcgaaagcaaccctatgggctttttcggtttcatacgtttcttacagccaggcacaaagtttaatttgaaagtggctttgatgcactctggcaacttaacaaagttcggtaaagacggcaaagcctctcctttctgggcaccatcgccctctcagctgaacaaagccctgtgggatttgagtgtcaaacttcccatagagatagaagactgataaactctcaaacaacagaaacttatataacaataacccattaataaaaaacaata Mask Tandem Repeat Region ================================================ tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcttctatctctatgggaagtttgacactcaaatcccacagggctttgttcagctgagagggcgatggtgcccagaaaggagaggctttgccgtctttaccgaactttgttaagttgccagagtgcatcaaagccactttcaaattaaactttgtgcctggctgtaagaaacgtatgaaaccgaaaaagcccatagggttgctttcgccgatgaactttcctttgtatgaatcggcgtaagtgtattcgtaaggcagctttgtgcggtctcttaccaatacattggccgtgccgttgtttacataatacataaagaaatgctggtgaatcttgtcttgttcgttatcgtagaattggtgattcatgtgctctccttttgcattgtagtagtccatacgcaaggaatagaccacatcggggtgcttcttcgtgttttttacaacgaatttggtctgtcctccgtcgcctgtcttgaagttttcgcctttcacaacttcccaaacaattttctgaacattgcctgtcggcacgaaatctgcctcttgtggcatattctcgaatgctgctccgctgcgcagtttgccctctgtgagtgtgaaaacagactgtgtagggtcttcgtgcaacttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Find is-nt database================================================ Query_seq: PI2052:PI2053|PI2052:PI2053:hypothetical protein:conserved hypothetical protein; possible outer membrane receptor protein:->->:2009757..2010812 1056 tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcttctatctctatgggaagtttgacactcaaatcccacagggctttgttcagctgagagggcgatggtgcccagaaaggagaggctttgccgtctttaccgaactttgttaagttgccagagtgcatcaaagccactttcaaattaaactttgtgcctggctgtaagaaacgtatgaaaccgaaaaagcccatagggttgctttcgccgatgaactttcctttgtatgaatcggcgtaagtgtattcgtaaggcagctttgtgcggtctcttaccaatacattggccgtgccgttgtttacataatacataaagaaatgctggtgaatcttgtcttgttcgttatcgtagaattggtgattcatgtgctctccttttgcattgtagtagtccatacgcaaggaatagaccacatcggggtgcttcttcgtgttttttacaacgaatttggtctgtcctccgtcgcctgtcttgaagttttcgcctttcacaacttcccaaacaattttctgaacattgcctgtcggcacgaaatctgcctcttgtggcatattctcgaatgctgctccgctgcgcagtttgccctctgtgagtgtgaaaacagactgtgtagggtcttcgtgcaacttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI2052:PI2053|PI2052:PI2053:hypothetical protein:conserved hypothetical protein; possible outer membrane receptor protein:->->:2009757..2010812 1056 tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcttctatctctatgggaagtttgacactcaaatcccacagggctttgttcagctgagagggcgatggtgcccagaaaggagaggctttgccgtctttaccgaactttgttaagttgccagagtgcatcaaagccactttcaaattaaactttgtgcctggctgtaagaaacgtatgaaaccgaaaaagcccatagggttgctttcgccgatgaactttcctttgtatgaatcggcgtaagtgtattcgtaaggcagctttgtgcggtctcttaccaatacattggccgtgccgttgtttacataatacataaagaaatgctggtgaatcttgtcttgttcgttatcgtagaattggtgattcatgtgctctccttttgcattgtagtagtccatacgcaaggaatagaccacatcggggtgcttcttcgtgttttttacaacgaatttggtctgtcctccgtcgcctgtcttgaagttttcgcctttcacaacttcccaaacaattttctgaacattgcctgtcggcacgaaatctgcctcttgtggcatattctcgaatgctgctccgctgcgcagtttgccctctgtgagtgtgaaaacagactgtgtagggtcttcgtgcaacttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI2052:PI2053|PI2052:PI2053:hypothetical protein:conserved hypothetical protein; possible outer membrane receptor protein:->->:2009757..2010812 1056 tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcttctatctctatgggaagtttgacactcaaatcccacagggctttgttcagctgagagggcgatggtgcccagaaaggagaggctttgccgtctttaccgaactttgttaagttgccagagtgcatcaaagccactttcaaattaaactttgtgcctggctgtaagaaacgtatgaaaccgaaaaagcccatagggttgctttcgccgatgaactttcctttgtatgaatcggcgtaagtgtattcgtaaggcagctttgtgcggtctcttaccaatacattggccgtgccgttgtttacataatacataaagaaatgctggtgaatcttgtcttgttcgttatcgtagaattggtgattcatgtgctctccttttgcattgtagtagtccatacgcaaggaatagaccacatcggggtgcttcttcgtgttttttacaacgaatttggtctgtcctccgtcgcctgtcttgaagttttcgcctttcacaacttcccaaacaattttctgaacattgcctgtcggcacgaaatctgcctcttgtggcatattctcgaatgctgctccgctgcgcagtttgccctctgtgagtgtgaaaacagactgtgtagggtcttcgtgcaacttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 63 Max: 686 Len: 624 Subject: UniRef90_Q64QQ3 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis HSP 1 e-value: 7.0E-23 bit: 110.0 Len: 621 Query Start:66 Query End:686 Subject Strand: null Subject Start: 40 Subject End: 271 ................................................................. L H E D P T Q S V F T L T E G K L R S - - - - - - - - G A A F E N M P Q E A D F V P T G N V Q K I V W E V V K G E N F K T G D G G Q T K F V V K N T K K H - - - - - - - P D V V Y S L R M D Y Y N A K G E H M N H Q F Y D N E Q D K I H Q H F F - - - - M Y Y V N N G T A N V L V R D R T K L P Y E Y T Y A D - - - - - - - - - S Y K G K F I G E S N P M G F F G F I R F L Q P G T K F N L K V A L M H S G N L T K F G K D G K A S P F W A P S P S Q L N K A L W D L S V K L P I E I .................................................................................................................................................................................................................................................................................................................................................................................. ................................................................. L H E D P A K V T V R L V E C H L H A D W N E I Q T N G G P H Q N P E S P A R H I K - - R I Q D I T Y E L K A G Q G W T L A E G S Q K K F Y V Q K N G E Y K N Q G R F T P A P V Y L M F I Y Y Y N A K G E L M N N Q F V E N G Q E N I H Q H F F T P E N I K P T F D G Q I E A D D N D P Q K L - I D Y L Y V D T T P W D K T Y H S G E A E I T G R D N P V G L K G I I R F L K D R K E F D L K V R L Y H G Y E S K K N P Q T G T F D P F Y K P S G V L I Q R G T W D I N L N L P V V V .................................................................................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q7MTQ7 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 1.0E-7 bit: 60.1 Len: 621 Query Start:63 Query End:683 Subject Strand: null Subject Start: 30 Subject End: 218 .............................................................. H E D P T Q S V F T L T E G K L R S G A A F E N M P Q E A D F V P T G N V Q K I V W E V V K G E - - N F K T G D G G Q T K F V V K N T K K H P D V V Y S L R M D Y Y N A K G E H M N H Q F Y D N E Q D K I H Q H F F M Y Y V N N G T A N V L V R D R T K L P - - Y E Y T Y A D S Y K G K F I G E S N P M G F F G F I R F L Q P G T K F N L K V A L M H S G N L T K F G K D G K A S P F W A P S P S Q L N K A L W D L S V K L P I E I E ..................................................................................................................................................................................................................................................................................................................................................................................... .............................................................. H D I P S Q L V C R F T E G V L T E P A D N S I N V S P T A F F S K D T V S - - I H H M V S N A F G S F A A L P G S Q K P K L K K G - - - - - - I W Y K M E I Y L F D K E N N R L N Q Q F F Q P D Q I E I H Q F F F N - - - - - - - - - - L M S N E T E V P K G I S Y Y Y S D V I N G Q L L - - E T P V G F T G Y I R V N K E L E A P E L R L M L V H L K T G N K Y E A D G K P N P F D K P S P R V L E - - F G D F A A F I G I E I K ..................................................................................................................................................................................................................................................................................................................................................................................... tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 16 End: 114 ............... M G Y C Y I S F C C L R V Y Q S S I S M G S L T L K S H R A L F S .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 56 Strand: + Start: 3 End: 170 .. M F F I N G L L L Y K F L L F E S L S V F Y L Y G K F D T Q I P Q G F V Q L R G R W C P E R R G F A V F T E L C ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 148 End: 297 ................................................................................................................................................... M P S L P N F V K L P E C I K A T F K L N F V P G C K K R M K P K K P I G L L S P M N F P L Y E S A ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 413 End: 532 ............................................................................................................................................................................................................................................................................................................................................................................................................................ M G D S C A L L L H C S S P Y A R N R P H R G A S S C F L Q R I W S V L R R L S ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 291 End: 755 .................................................................................................................................................................................................................................................................................................. M G V S V F V R Q L C A V S Y Q Y I G R A V V Y I I H K E M L V N L V L F V I V E L V I H V L S F C I V V V H T Q G I D ............................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 577 End: 840 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M P V G T K S A S C G I F S N A A P L R S L P S V S V K T D C V G S S C N L F S F S S S G F S G L Q A E K K P I L S I P ........................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 870 End: 1055 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y C C C N L L V I Y K R I P R V G Y E H I P R V G C K C I S R V V Y K R T L L V G C E S G L A H D E T T N K F I E L T . Protein_Len: 58 Strand: - Start: 875 End: 1048 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q Q Q L R S T I Y L R I G R T P Y S C I G R T P H L H I D R T T Y L R V K S T P H S L P K A C S S V V L L N M S S M ........ Protein_Len: 60 Strand: - Start: 838 End: 1035 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Y N N T F K N N S Y G V R S I C V Y V V L P T R A Y V V L P T C T Y I V L P I Y V C R V Q L T H F R N L V R H F L W Y M ..................... Protein_Len: 60 Strand: - Start: 551 End: 742 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L K R F M A Q R C S I Q R K H C I R S H Q E A A C N A R Q S H S F L S H L T K T C S T K R R T K Q S K L I A L L S S V M .......................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 406 End: 621 ..................................................................................................................................................................................................................................................................................................................................................................................................................... Q L L G Y A L F L G C R P A E E H K K C R I Q D T R R R R D Q L K R R E C S G L C N E S C Q R D A R F R G R T A Y E R M ................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 208 End: 339 ............................................................................................................................................................................................................... V K H R A T L F T H F R F L G Y P Q K R R H V K R Q I F R R L H I R L A A K H P R K G M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 30 Strand: - Start: 203 End: 292 .......................................................................................................................................................................................................... I L S Q A Q S Y S V Y S V S F A W L T A K A S S S E K T H M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 60 End: 806 ........................................................... G S R L K G E T L T F V S Q T P D E H L K N E K E D E P K E P N C A S F F G I S L M G L L I P F I S N K I K M K K S K M .......................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: - Start: 16 End: 135 ............... H T I T I Y T E T T Q S N I L R R D R H S T Q C E F G V P S Q E A S L A I T G M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 794 End: 823 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTACTTTTTATTGTTTACTTATTATACT......................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 91 HP_score: -13.6 Tail_Score: -2.52464 Start: 891 End: 918 Full_Region: tatgctcgtacccca ctcgtggtatac gttt gtatatcacgag taggttacagcaaca ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctcgtggtatacgtttgtatatcacgag.......................................................................................................................................... Find igs database================================================ Query_seq: PI2052:PI2053|PI2052:PI2053:hypothetical protein:conserved hypothetical protein; possible outer membrane receptor protein:->->:2009757..2010812 1056 tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactgtttttaatcttcatctttttactttttattgtttacttattatacttatgtctttatttaatgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta Intra-Species Hit: Count: 1 Min: 1 Max: 1056 Len: 1056 Subject: pint_PI2052_PI2053|hypothetical protein:conserved hypothetical protein; possible outer membrane receptor protein|POSITIVE:POSITIVE|[2009757,2010812]|1056 HSP 1 e-value: 1.0E-120 bit: 434.0 Len: 219 Query Start:838 Query End:1056 Subject Strand: POSITIVE Subject Start: 838 Subject End: 1056 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atgtgagttcgacgaactgtaattgtttgtaaatttattgttgctgtaacctactcgtgatatacaaacgtataccacgagtggggtacgagcatataccacgagtggggtgcaagtgtatatcacgagtggtatataaacgcaccttacttgtagggtgtgaaagcggtttagcacacgatgaaacaaccaataaattcattgaactcacgttattta HSP 2 e-value: 7.0E-44 bit: 180.0 Len: 91 Query Start:687 Query End:777 Subject Strand: POSITIVE Subject Start: 687 Subject End: 777 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactg....................................................................................................................................................................................................................................................................................... ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttgttttccttctcgtcttctggcttttcaggattgcaagcagagaagaaaccaatactgagcatacctaataaaataggaaaaatactg....................................................................................................................................................................................................................................................................................... HSP 3 e-value: 1.0E-26 bit: 123.0 Len: 62 Query Start:1 Query End:62 Subject Strand: POSITIVE Subject Start: 1 Subject End: 62 tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tattgttttttattaatgggttattgttatataagtttctgttgtttgagagtttatcagtc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUGUUUUUUAUUAAUGGGUUAUUGUUAUAUAAGUUUCUGUUGUUUGAGAGUUUAUCAGUCUUCUAUCUCUAUGGGAAGUUUGACACUCAAAUCCCACAGGGCUUUGUUCAGCUGAGAGGGCGAUGGUGCCCAGAAAGGAGAGGCUUUGCCGUCUUUACCGAACUUUGUUAAGUUGCCAGAGUGCAUCAAAGCCACUUUCAAAUUAAACUUUGUGCCUGGCUGUAAGAAACGUAUGAAACCGAAAAAGCCCAUAGGGUUGCUUUCGCCGAUGAACUUUCCUUUGUAUGAAUCGGCGUAAGUGUAUUCGUAAGGCAGCUUUGUGCGGUCUCUUACCAAUACAUUGGCCGUGCCGUUGUUUACAUAAUACAUAAAGAAAUGCUGGUGAAUCUUGUCUUGUUCGUUAUCGUAGAAUUGGUGAUUCAUGUGCUCUCCUUUUGCAUUGUAGUAGUCCAUACGCAAGGAAUAGACCACAUCGGGGUGCUUCUUCGUGUUUUUUACAACGAAUUUGGUCUGUCCUCCGUCGCCUGUCUUGAAGUUUUCGCCUUUCACAACUUCCCAAACAAUUUUCUGAACAUUGCCUGUCGGCACGAAAUCUGCCUCUUGUGGCAUAUUCUCGAAUGCUGCUCCGCUGCGCAGUUUGCCCUCUGUGAGUGUGAAAACAGACUGUGUAGGGUCUUCGUGCAACUUGUUUUCCUUCUCGUCUUCUGGCUUUUCAGGAUUGCAAGCAGAGAAGAAACCAAUACUGAGCAUACCUAAUAAAAUAGGAAAAAUACUGUUUUUAAUCUUCAUCUUUUUACUUUUUAUUGUUUACUUAUUAUACUUAUGUCUUUAUUUAAUGUGAGUUCGACGAACUGUAAUUGUUUGUAAAUUUAUUGUUGCUGUAACCUACUCGUGAUAUACAAACGUAUACCACGAGUGGGGUACGAGCAUAUACCACGAGUGGGGUGCAAGUGUAUAUCACGAGUGGUAUAUAAACGCACCUUACUUGUAGGGUGUGAAAGCGGUUUAGCACACGAUGAAACAACCAAUAAAUUCAUUGAACUCACGUUAUUUA .................((((((............((((((((.....((.((...(((((.((((.(((((.(((((..((((.....)))))))))..((((..(((((((..(((((((((((((.(..((((..((((((((...))).))))).(((((.(((((......(((((...(((.(((((................))))))))))))).(((((((((..(((((..(((((.(((((...)))))..)))))((((((..((((((((((((((...((((...((((((((.((((((..((.....))....)))))).)))))))).)))).........(((((((((.((.((((....((..(((((((....((.((((.........)))).)).)))))))..))....)))))).))))).))))..)))))).)))))).))....))))))........)))))))))))))).))))))))))))))..).))))))))).))))((((((............))))))............)))))))..))))....(((((((...........(..((((........))))..).((.((((((((((((...((.........))...))))))))))))))..)))))))..(((((..((((....(((....))).....))))..))))).)))))))))......)))))....)))).....))))))))............))))))............................................((..((((((((((((((.(((.(...((((.((((...(((((((((((((...((((((((((.(((((....))))).))))))))))...((..(((((.(((((((((((((((...(((((((((....)))))))))...))))))))))))).)))))))....))...))))).)))))))))))).)))).).))).))))))))))))))..)) (-321.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAACGUGAGUUCAAUGAAUUUAUUGGUUGUUUCAUCGUGUGCUAAACCGCUUUCACACCCUACAAGUAAGGUGCGUUUAUAUACCACUCGUGAUAUACACUUGCACCCCACUCGUGGUAUAUGCUCGUACCCCACUCGUGGUAUACGUUUGUAUAUCACGAGUAGGUUACAGCAACAAUAAAUUUACAAACAAUUACAGUUCGUCGAACUCACAUUAAAUAAAGACAUAAGUAUAAUAAGUAAACAAUAAAAAGUAAAAAGAUGAAGAUUAAAAACAGUAUUUUUCCUAUUUUAUUAGGUAUGCUCAGUAUUGGUUUCUUCUCUGCUUGCAAUCCUGAAAAGCCAGAAGACGAGAAGGAAAACAAGUUGCACGAAGACCCUACACAGUCUGUUUUCACACUCACAGAGGGCAAACUGCGCAGCGGAGCAGCAUUCGAGAAUAUGCCACAAGAGGCAGAUUUCGUGCCGACAGGCAAUGUUCAGAAAAUUGUUUGGGAAGUUGUGAAAGGCGAAAACUUCAAGACAGGCGACGGAGGACAGACCAAAUUCGUUGUAAAAAACACGAAGAAGCACCCCGAUGUGGUCUAUUCCUUGCGUAUGGACUACUACAAUGCAAAAGGAGAGCACAUGAAUCACCAAUUCUACGAUAACGAACAAGACAAGAUUCACCAGCAUUUCUUUAUGUAUUAUGUAAACAACGGCACGGCCAAUGUAUUGGUAAGAGACCGCACAAAGCUGCCUUACGAAUACACUUACGCCGAUUCAUACAAAGGAAAGUUCAUCGGCGAAAGCAACCCUAUGGGCUUUUUCGGUUUCAUACGUUUCUUACAGCCAGGCACAAAGUUUAAUUUGAAAGUGGCUUUGAUGCACUCUGGCAACUUAACAAAGUUCGGUAAAGACGGCAAAGCCUCUCCUUUCUGGGCACCAUCGCCCUCUCAGCUGAACAAAGCCCUGUGGGAUUUGAGUGUCAAACUUCCCAUAGAGAUAGAAGACUGAUAAACUCUCAAACAACAGAAACUUAUAUAACAAUAACCCAUUAAUAAAAAACAAUA ........((((((((.(((((((.((((.((((.......((((((.((((.....................((((((..((((((((((..(((................)))..))))))))))..))))))..(((((((((((((....))))))))))))).))))..))).)))........)))).))))...))))))).)))))))).............((((((....(((((............((((...((((((..........(((((....((((......)))).))))).....(((((((.(((((((.((((....(((((..(((.......(((...(((((((..(((...............)))..)))))))...)))......((((..((((........))))....(((((...((((......))))..)))))))))....)))....))))).....(((((..((((((............)))))).))))).))))))))))).))))))).))))))(((..((((.(((((((((.((.(((((....((.((((((..(((((((.(((...((((((.(((((((((...((((((..........((....))..........))))))...)).))))))).))))))..)))..........((((...((((((.(((((.(.(.((.....)).))))))).)))))).....)))).))))))).))))))))..)))))((....))........)))))))))))))))..))).....)))).(((((((((((((((............))))))).)))...))))).))))).....(((((((..(((.(((...)))))).......((((......)))).....)))))))......((((..(.(((((((((((..((((...........))))..)))))....))))))).))))..)))))).............................. (-297.36) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 531 949 907 187 145 pint:PI1227|5end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148448..1148678_POSITIVE 470 140 92 59 5 pint:PI1778|5end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1710009..1710239_POSITIVE 422 152 115 177 141 pint:PI1187|5end_conserved_hypothetical_protein_1106692..1106922_POSITIVE 422 669 626 53 16 pint:PI0221|5end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_212356..212586_POSITIVE 418 152 111 67 11 pint:PI1816|5end_acid_phosphatase_1754646..1754876_POSITIVE 414 630 581 167 113 pint:PI1980|5end_conserved_hypothetical_protein_1937470..1937700_POSITIVE 411 190 141 59 11 pint:PI0038|5end_transcriptional_regulator,_LysR_family_39578..39808_POSITIVE 405 155 111 58 5 pint:PI0328|5end_conserved_hypothetical_protein_309476..309706_POSITIVE 402 670 621 59 3 pint:PI1892|5end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_1834217..1834447_POSITIVE 400 146 114 229 199 pint:PI0899|5end_methylenetetrahydrofolate_dehydrogenase/methenyltetrahydrofolate_cyclohydrolase_828738..828968_POSITIVE 390 639 591 50 2 pint:PI2158|5end_glycosyl_transferase,_group_2_family_protein_2104392..2104622_POSITIVE 388 330 281 55 1 pint:PI1780|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_1711866..1712096_POSITIVE 387 960 912 91 48 pint:PI2139|5end_cysteinyl-tRNA_synthetase_2085266..2085496_POSITIVE 380 948 907 105 57 pint:PI2104|5end_carboxyl-terminal_protease_2057910..2058140_POSITIVE 379 638 595 104 60 pint:PI0703|5end_hypothetical_protein_639655..639885_POSITIVE 373 657 615 164 117 pint:PI0854|5end_MiaB-like_tRNA_modifying_enzyme_786513..786743_POSITIVE 371 638 591 86 46 pint:PI0591|5end_adenylosuccinate_lyase_538595..538825_POSITIVE 368 620 571 220 167 pint:PI1224|5end_sensor_histidine_kinase_and_response_regulator_1144574..1144804_POSITIVE 366 152 112 51 3 pint:PI0261|5end_conjugative_transposon_protein,_TraK_254065..254295_POSITIVE 366 280 231 68 30 pint:PI0068|5end_arginine_repressor_66586..66816_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 450 140 95 51 2 pint:PI1777|3end_conserved_hypothetical_protein_1710081..1710231_POSITIVE 428 947 902 76 23 pint:PI1475|3end_possible_short_chain_dehydrogenase_1397905..1398055_POSITIVE 400 146 114 141 111 pint:PI0898|3end_conserved_hypothetical_protein_828730..828880_POSITIVE 396 630 583 123 66 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 380 948 907 49 1 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 366 610 570 126 94 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 364 338 292 135 92 pint:PI0935|3end_collagenase,_protease_868320..868470_POSITIVE 361 356 313 101 52 pint:PI1484|3end_conserved_hypothetical_protein_1405223..1405373_POSITIVE 359 154 116 49 3 pint:PI0778|3end_hypothetical_protein_714885..715035_POSITIVE 358 646 603 51 3 pint:PI0341|3end_conserved_hypothetical_protein_317615..317765_POSITIVE 357 352 312 147 95 pint:PI0345|3end_conserved_hypothetical_protein_320809..320959_POSITIVE 355 960 916 77 35 pint:PI2158|3end_glycosyl_transferase,_group_2_family_protein_2105347..2105497_POSITIVE 355 148 111 111 68 pint:PI0115|3end_hypothetical_protein_108669..108819_POSITIVE 354 970 925 50 5 pint:PI1940|3end_conserved_hypothetical_protein;_possible_outer_membrane_protein_1897267..1897417_POSITIVE 348 196 151 76 20 pint:PI2016|3end_ribosomal_large_subunit_pseudouridine_synthase_D_1974933..1975083_POSITIVE 348 930 881 78 31 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 347 702 661 108 68 pint:PI0351|3end_hypothetical_protein_324768..324918_POSITIVE 345 140 93 110 72 pint:PI1663|3end_hypothetical_protein_1577347..1577497_POSITIVE 345 529 481 54 2 pint:PI0958|3end_G:T/U_mismatch-specific_DNA_glycosylase_890569..890719_POSITIVE 343 146 101 143 102 pint:PI2095|3end_hypothetical_protein_2050216..2050366_POSITIVE