Origin IGS: tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatttaactctgtttagtaagttgttaatataaagtctatattctttaaaaatagtttaagtgcagatggaatagtctgctatgtttgcaaatatactaaaaactctaaacattcagaattattccgtcgttttttactaattttgcctataatacttaattttcgcccaccttttatagcggggcagataatttttagaaataaagacaatgaattttaagaagttctttttagtagcaggcttagcagccaccaca Mask Tandem Repeat Region ================================================ tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Find is-nt database================================================ Query_seq: PI1983:PI1984|PI1983:PI1984:possible xylanase:MutT/nudix family protein:->->:1940065..1940323 259 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1983:PI1984|PI1983:PI1984:possible xylanase:MutT/nudix family protein:->->:1940065..1940323 259 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1983:PI1984|PI1983:PI1984:possible xylanase:MutT/nudix family protein:->->:1940065..1940323 259 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 2 End: 118 . M V A A K P A T K K N F L K F I V F I S K N Y L P R Y K R W A K I K Y Y R Q N ............................................................................................................................................. Protein_Len: 43 Strand: + Start: 48 End: 176 ............................................... M S L F L K I I C P A I K G G R K L S I I G K I S K K R R N N S E C L E F L V Y L Q T ................................................................................... Protein_Len: 60 Strand: - Start: 41 End: 229 ........................................ D K N R F I I Q G A I F P P R F N L I I P L I L L F R R F L E S H K S N K T Y K C V Y C V I G D A S L S N K F F I S K M .............................. tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 47 HP_score: -3.4 Tail_Score: -3.25472 Start: 179 End: 196 Full_Region: aaaaatagtttaagt gcagat ggaata gtctgc tatgtttgcaaatat ..................................................................................................................................................................................gcagatggaatagtctgc............................................................... Find igs database================================================ Query_seq: PI1983:PI1984|PI1983:PI1984:possible xylanase:MutT/nudix family protein:->->:1940065..1940323 259 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Intra-Species Hit: Count: 1 Min: 1 Max: 259 Len: 259 Subject: pint_PI1983_PI1984|possible xylanase:MutT/nudix family protein|POSITIVE:POSITIVE|[1940065,1940323]|259 HSP 1 e-value: 1.0E-145 bit: 513.0 Len: 259 Query Start:1 Query End:259 Subject Strand: POSITIVE Subject Start: 1 Subject End: 259 tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca tgtggtggctgctaagcctgctactaaaaagaacttcttaaaattcattgtctttatttctaaaaattatctgccccgctataaaaggtgggcgaaaattaagtattataggcaaaattagtaaaaaacgacggaataattctgaatgtttagagtttttagtatatttgcaaacatagcagactattccatctgcacttaaactatttttaaagaatatagactttatattaacaacttactaaacagagttaaatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGGUGGCUGCUAAGCCUGCUACUAAAAAGAACUUCUUAAAAUUCAUUGUCUUUAUUUCUAAAAAUUAUCUGCCCCGCUAUAAAAGGUGGGCGAAAAUUAAGUAUUAUAGGCAAAAUUAGUAAAAAACGACGGAAUAAUUCUGAAUGUUUAGAGUUUUUAGUAUAUUUGCAAACAUAGCAGACUAUUCCAUCUGCACUUAAACUAUUUUUAAAGAAUAUAGACUUUAUAUUAACAACUUACUAAACAGAGUUAAAUCA .(((((((((....)))).))))).................(((((.((((((.((...((...((((.((.((((.(((......))))))))).)))).))...)).))))))................((((.....))))..(((((((((((.((((((((............(((((........))))).......((((.(((...))).))))....)))))))).))))..))))))))))))...... (-51.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUUAACUCUGUUUAGUAAGUUGUUAAUAUAAAGUCUAUAUUCUUUAAAAAUAGUUUAAGUGCAGAUGGAAUAGUCUGCUAUGUUUGCAAAUAUACUAAAAACUCUAAACAUUCAGAAUUAUUCCGUCGUUUUUUACUAAUUUUGCCUAUAAUACUUAAUUUUCGCCCACCUUUUAUAGCGGGGCAGAUAAUUUUUAGAAAUAAAGACAAUGAAUUUUAAGAAGUUCUUUUUAGUAGCAGGCUUAGCAGCCACCACA ..........(((((.(((..((((((((......((((.((((.(((((((((...(((((((..((((((((((((((..(((((((..................))))))).))).)))))))))))........(.......).......))))))).....((((..((.....))..)))).....))))))))))))).))))...((((((....))))))...))))))))..))).)))))........ (-55.67) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 89 48 224 181 pint:PI0117|5end_ribonuclease_HI_110731..110961_POSITIVE 293 94 72 229 203 pint:PI0882|5end_conserved_hypothetical_protein;_possible_para-aminobenzoate_synthase_component_I_815545..815775_POSITIVE 265 114 86 67 36 pint:PI1378|5end_GTP-binding_protein_lepA_1299904..1300134_POSITIVE 261 33 1 99 68 pint:PI1950|5end_iron-regulated_membrane_permease_1904468..1904698_POSITIVE 259 93 66 44 15 pint:PI0093|5end_riboflavin_biosynthesis_protein_90489..90719_POSITIVE 255 91 61 207 170 pint:PI1912|5end_possible_DNA_repair_protein_1856364..1856594_POSITIVE 254 38 7 61 19 pint:PI1799|5end_30S_ribosomal_protein_S2_1732900..1733130_POSITIVE 254 200 167 36 4 pint:PI0684|5end_coproporphyrinogen_III_oxidase_619357..619587_POSITIVE 254 25 5 90 68 pint:PI0489|5end_probable_cardiolipin_synthetase_447610..447840_POSITIVE 253 23 1 60 37 pint:PI1625|5end_conserved_hypothetical_protein;_possible_phage_transposase_1549316..1549546_POSITIVE 253 23 1 60 37 pint:PI0332|5end_conserved_hypothetical_protein_312600..312830_POSITIVE 253 120 73 127 70 pint:PI0169|5end_hypothetical_protein_163645..163875_POSITIVE 250 34 1 116 83 pint:PI1994|5end_hypothetical_protein_1949360..1949590_POSITIVE 250 38 4 201 164 pint:PI0718|5end_hypothetical_protein_653725..653955_POSITIVE 250 97 65 86 41 pint:PI0670|5end_hypothetical_protein_611000..611230_POSITIVE 247 95 73 213 188 pint:PI2112|5end_phosphopantetheine_adenylyltransferase_2063194..2063424_POSITIVE 247 113 73 49 10 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 245 114 71 146 96 pint:PI0224|5end_conserved_hypothetical_protein_220541..220771_POSITIVE 242 112 71 178 134 pint:PI0520|5end_hypothetical_protein_478755..478985_POSITIVE 242 50 1 104 50 pint:PI0027|5end_aminopeptidase_C_30468..30698_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 265 114 86 42 11 pint:PI1377|3end_cAMP-binding_domain_protein_(cyclic_nucleotide-binding_domain)_1299959..1300109_POSITIVE 261 33 1 81 50 pint:PI1949|3end_aminotransferase,_class_V_1904530..1904680_POSITIVE 260 190 142 81 20 pint:PI0673|3end_hypothetical_protein_612705..612855_POSITIVE 253 120 73 101 44 pint:PI0167|3end_hypothetical_protein_163699..163849_POSITIVE 245 114 71 108 58 pint:PI0223|3end_topoisomerase_IA_220583..220733_POSITIVE 242 50 1 93 39 pint:PI0026|3end_two-component_system_response_regulator_30537..30687_POSITIVE 242 116 71 99 49 pint:PI0005|3end_hypothetical_protein_7450..7600_POSITIVE 237 26 3 24 1 pint:PI1650|3end_N-acetylmuramoyl-L-alanine_amidase_1563901..1564051_POSITIVE 236 22 2 52 29 pint:PI1080|3end_conserved_hypothetical_protein;_possible_outer_membrane_lipoprotein_1000883..1001033_POSITIVE 233 114 71 68 22 pint:PI0267|3end_conjugative_transposon_protein,_TraQ_259291..259441_POSITIVE 232 95 65 151 108 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 230 118 73 33 4 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 229 206 179 121 94 pint:PI0892|3end_acyl-ACP_thioesterase_823939..824089_POSITIVE 228 94 70 145 114 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 226 186 145 62 20 pint:PI0306|3end_conserved_hypothetical_protein_289828..289978_POSITIVE 226 96 66 142 104 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 225 37 4 134 100 pint:PI1726|3end_ATP-dependent_DNA_helicase_1645542..1645692_POSITIVE 225 97 51 150 108 pint:PI0426|3end_conserved_hypothetical_protein_391206..391356_POSITIVE 224 95 71 135 111 pint:PI1806|3end_integral_membrane_transport_protein;_possible_sugar_transport_proteins_1742191..1742341_POSITIVE 224 114 71 102 65 pint:PI1440|3end_conserved_hypothetical_protein_1363009..1363159_POSITIVE