Origin IGS: cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctattagaaatagcagactgttttttgcaggaataaagccatatagaaagaaaaagtataaaacatttggtagactgcggatttattgctaactttgcatcgttcagtggaatgaggagcttttaggagttcctcatttttcatattttaaggtgggataggcaatggttttaaattaaaaactgtcccacggtaaaattgtaaaagttcg Mask Tandem Repeat Region ================================================ cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Find is-nt database================================================ Query_seq: PI1956:PI1957|PI1956:PI1957:conserved hypothetical protein:calcium-transporting ATPase:->->:1913687..1913898 212 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1956:PI1957|PI1956:PI1957:conserved hypothetical protein:calcium-transporting ATPase:->->:1913687..1913898 212 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1956:PI1957|PI1956:PI1957:conserved hypothetical protein:calcium-transporting ATPase:->->:1913687..1913898 212 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 68 End: 205 ................................................................... M K N E E L L K A P H S T E R C K V S N K S A V Y Q M F Y T F S F Y M A L F L Q K T V C Y F ....... Protein_Len: 38 Strand: + Start: 99 End: 212 .................................................................................................. M P L N D A K L A I N P Q S T K C F I L F L S I W L Y S C K K Q S A I S N R cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 90 HP_score: -8.2 Tail_Score: -4.98616 Start: 76 End: 99 Full_Region: catcgttcagtggaa tgaggagctt ttag gagttcctca tttttcatattttaa ...........................................................................tgaggagcttttaggagttcctca................................................................................................................. Find igs database================================================ Query_seq: PI1956:PI1957|PI1956:PI1957:conserved hypothetical protein:calcium-transporting ATPase:->->:1913687..1913898 212 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Intra-Species Hit: Count: 1 Min: 1 Max: 212 Len: 212 Subject: pint_PI1956_PI1957|conserved hypothetical protein:calcium-transporting ATPase|POSITIVE:POSITIVE|[1913687,1913898]|212 HSP 1 e-value: 1.0E-117 bit: 420.0 Len: 212 Query Start:1 Query End:212 Subject Strand: POSITIVE Subject Start: 1 Subject End: 212 cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga cgaacttttacaattttaccgtgggacagtttttaatttaaaaccattgcctatcccaccttaaaatatgaaaaatgaggaactcctaaaagctcctcattccactgaacgatgcaaagttagcaataaatccgcagtctaccaaatgttttatactttttctttctatatggctttattcctgcaaaaaacagtctgctatttctaataga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAACUUUUACAAUUUUACCGUGGGACAGUUUUUAAUUUAAAACCAUUGCCUAUCCCACCUUAAAAUAUGAAAAAUGAGGAACUCCUAAAAGCUCCUCAUUCCACUGAACGAUGCAAAGUUAGCAAUAAAUCCGCAGUCUACCAAAUGUUUUAUACUUUUUCUUUCUAUAUGGCUUUAUUCCUGCAAAAAACAGUCUGCUAUUUCUAAUAGA ..........((((((((..(((((((((((((......)))))...))....))))))..)))))).))...((((((((.((......)).))))))))..((((.....(((.......)))........((((..........((..((((...........))))..)).......))))......))))...((((.....)))). (-32.23) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAUUAGAAAUAGCAGACUGUUUUUUGCAGGAAUAAAGCCAUAUAGAAAGAAAAAGUAUAAAACAUUUGGUAGACUGCGGAUUUAUUGCUAACUUUGCAUCGUUCAGUGGAAUGAGGAGCUUUUAGGAGUUCCUCAUUUUUCAUAUUUUAAGGUGGGAUAGGCAAUGGUUUUAAAUUAAAAACUGUCCCACGGUAAAAUUGUAAAAGUUCG (((((((((....(((((......))))).((.......)).........................)))))))))...(((((((.((((.((.(((((........(..(((((((((((((....)))))))))))))..)..........(((((((((....((((.....))))....))))))))).))))).))))))))))))) (-54.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 115 79 227 187 pint:PI1636|5end_hypothetical_protein_1554246..1554476_POSITIVE 292 135 91 156 113 pint:PI1525|5end_CDP-diacylglycerol--serine_O-phosphatidyltransferase_1446758..1446988_POSITIVE 290 88 43 156 104 pint:PI0709|5end_possible_IS1249-related_transposase_646467..646697_POSITIVE 277 120 86 78 34 pint:PI0842|5end_5-formyltetrahydrofolate_cyclo-ligase_775533..775763_POSITIVE 271 138 92 151 105 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 268 178 135 149 106 pint:PI1265|5end_hypothetical_protein_1185016..1185246_POSITIVE 264 137 91 112 54 pint:PI0062|5end_endopeptidase_60963..61193_POSITIVE 260 138 99 190 153 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 260 61 21 164 120 pint:PI0932|5end_conserved_hypothetical_protein;_possible_hemin_receptor_863854..864084_POSITIVE 253 143 101 77 30 pint:PI2014|5end_conserved_hypothetical_protein_1971471..1971701_POSITIVE 251 160 118 188 149 pint:PI0803|5end_DNA-damage-inducible_protein_F_739757..739987_POSITIVE 251 124 92 171 144 pint:PI0338|5end_hypothetical_protein_315337..315567_POSITIVE 249 60 19 67 24 pint:PI0035|5end_NAD-dependent_protein_deacetylase,_SIR2_family_37873..38103_POSITIVE 247 147 102 192 145 pint:PI0435|5end_30S_ribosomal_protein_S21_406997..407227_POSITIVE 245 144 106 30 1 pint:PI1794|5end_conserved_hypothetical_protein_1725609..1725839_POSITIVE 245 87 44 140 88 pint:PI0924|5end_possible_glycosyl_hydrolase_855004..855234_POSITIVE 244 124 84 87 37 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 241 150 102 55 15 pint:PI1390|5end_hypothetical_protein_1311154..1311384_POSITIVE 241 149 105 120 77 pint:PI1292|5end_conserved_hypothetical_protein_1206106..1206336_POSITIVE 240 144 106 69 22 pint:PI0382|5end_conserved_hypothetical_protein_351510..351740_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 277 120 86 46 2 pint:PI0841|3end_hypothetical_protein_775581..775731_POSITIVE 268 178 135 127 84 pint:PI1264|3end_hypothetical_protein_1185074..1185224_POSITIVE 260 138 99 61 24 pint:PI1362|3end_60Kd_inner_membrane_protein_1281813..1281963_POSITIVE 253 143 101 81 34 pint:PI2012|3end_hypothetical_protein_1971555..1971705_POSITIVE 252 116 76 72 27 pint:PI0292|3end_ISPg-3_transposase_fragment_275904..276054_POSITIVE 245 87 44 107 55 pint:PI0923|3end_6-phosphofructokinase_855051..855201_POSITIVE 244 124 84 78 28 pint:PI2016|3end_ribosomal_large_subunit_pseudouridine_synthase_D_1974933..1975083_POSITIVE 242 199 154 120 75 pint:PI1165|3end_hypothetical_protein_1089636..1089786_POSITIVE 241 150 102 150 110 pint:PI1390|3end_hypothetical_protein_1311329..1311479_POSITIVE 240 206 162 107 66 pint:PI0745|3end_conserved_hypothetical_protein_675670..675820_POSITIVE 234 116 72 69 32 pint:PI0282|3end_hypothetical_protein_266826..266976_POSITIVE 232 117 72 71 14 pint:PI0706|3end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_643394..643544_POSITIVE 231 140 92 137 96 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 231 140 105 66 41 pint:PI0314|3end_conserved_hypothetical_protein_296678..296828_POSITIVE 228 139 92 84 41 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 226 133 91 149 98 pint:PI0196|3end_hypothetical_protein_188643..188793_POSITIVE 225 114 77 75 30 pint:PI1827|3end_peptidase,_M23/M37_family,_N-terminal_1768602..1768752_POSITIVE 225 59 19 125 95 pint:PI0179|3end_hypothetical_protein_171959..172109_POSITIVE 222 137 91 96 56 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 222 149 105 94 51 pint:PI0152|3end_DNA_mismatch_endonuclease_vsr_145984..146134_POSITIVE