Origin IGS: taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagggggagtaatcaactctctgacaatataaaaagcgtgtttgccattgggtaaacacgctttttggtttctatattattgttgtcagctaaaactaaagtataaatacaccttattatatcgagctcacgttgtaatcagatataaaacaaccttaaataaaaatatgtccaaattatttttgatataataaaatttgtattacctttgtaatggcattatagccaaagttctttataaatcaaatctgatatgaataaaaaatgtttcaagttggtattctcctttgtcactccaagtgaacttctaacagatgacgaaacttatgtcagatataatatacattgatatgaaatacttacgactattatcttggctactttgttgcactgttttaatgttttcattagcttcatgcgaagaaaaagagccagatttaacaaagaaagaaatggatactcgtttgctgggtacttggaagcaaataaattccaatatttcagaaaataagaaacttatatttatgtctaatggagacattattggttatgatttcgtaccgggagggaaaaagagagttttttatacagaaaataattgccatttatttgtatttgtaaagggtttgggtataaaattatctaattggacatacgaacattactataaaatagatggaaataagctaactttatggtatagtttgtatggaatgaattcaaatagttcagattgtcttatctatcagaaagaaaaataacggattattttcatttaattcttatatcaaactcacgtta Mask Tandem Repeat Region ================================================ taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Find is-nt database================================================ Query_seq: PI1934:PI1935|PI1934:PI1935:hypothetical protein:possible internalin-related protein:->->:1882662..1883463 802 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1934:PI1935|PI1934:PI1935:hypothetical protein:possible internalin-related protein:->->:1882662..1883463 802 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1934:PI1935|PI1934:PI1935:hypothetical protein:possible internalin-related protein:->->:1882662..1883463 802 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 160 End: 258 ............................................................................................................................................................... M I L Y P N P L Q I Q I N G N Y F L Y K K L S F S L P V R N H N Q ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 34 Strand: + Start: 197 End: 298 .................................................................................................................................................................................................... M A I I F C I K N S L F P S R Y E I I T N N V S I R H K Y K F L I F ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 59 Strand: + Start: 298 End: 474 ......................................................................................................................................................................................................................................................................................................... M K Y W N L F A S K Y P A N E Y P F L S L L N L A L F L R M K L M K T L K Q C N K V A K I I V V S I S Y Q C I L Y L T ........................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 258 End: 491 ................................................................................................................................................................................................................................................................. M M S P L D I N I S F L F S E I L E F I C F Q V P S K R V S I S F F V K S G S F S S H E A N E N I K T V Q Q S S Q D N S ....................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 682 End: 786 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I R C I Y T L V L A D N N N I E T K K R V Y P M A N T L F I L S E S ................ Protein_Len: 49 Strand: - Start: 614 End: 760 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I L F L K S M N K N L T T K Y R I V V H A R Y L L T Y K Y K L K L Q C C Y Y L F W F A H K G L P M .......................................... Protein_Len: 35 Strand: - Start: 456 End: 560 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... H I N Y R V Y T E D D T L L E S P T V F S F V L K F C K K N M D S K M .................................................................................................................................................................................................................................................. Protein_Len: 51 Strand: - Start: 294 End: 446 ..................................................................................................................................................................................................................................................................................................... K R F Y Q F K N A E L Y G A F S Y G N R E K N F R A R K R R M F S I F V N F C H L L T A L I I T T L M .................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 43 Strand: - Start: 184 End: 312 ....................................................................................................................................................................................... L Y L Y I A I I K Q I F F E R K G E R Y S I M V L L T E M L C L Y L N R I K Q F I P M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 44 End: 487 ........................................... S N I Q K W T G L L R T D M E K K T L D P E K E E C S A L S F M L V T C C L L W S L L R L Y K M D I Y I I D S M L K T M ........................................................................................................................................................................................................................................................................................................................... taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 554 End: 583 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTGATTTATAAAGAACTTTGGCTATAAT........................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.9 Tail_Score: -5.1523 Start: 741 End: 766 Full_Region: TATAGAAACCAAAAA GCGTGTTTACC CAAT GGCAAACACGC TTTTTATATTGTCAG ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCGTGTTTACCCAATGGCAAACACGC.................................... TransTerm Strand: - Conf: 100 HP_score: -14.1 Tail_Score: -5.07011 Start: 741 End: 766 Full_Region: ctgacaatataaaaa gcgtgtttgcc attg ggtaaacacgc tttttggtttctata ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcgtgtttgccattgggtaaacacgc.................................... Find igs database================================================ Query_seq: PI1934:PI1935|PI1934:PI1935:hypothetical protein:possible internalin-related protein:->->:1882662..1883463 802 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Intra-Species Hit: Count: 1 Min: 1 Max: 802 Len: 802 Subject: pint_PI1934_PI1935|hypothetical protein:possible internalin-related protein|POSITIVE:POSITIVE|[1882662,1883463]|802 HSP 1 e-value: 0.0 bit: 1590.0 Len: 802 Query Start:1 Query End:802 Subject Strand: POSITIVE Subject Start: 1 Subject End: 802 taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta taacgtgagtttgatataagaattaaatgaaaataatccgttatttttctttctgatagataagacaatctgaactatttgaattcattccatacaaactataccataaagttagcttatttccatctattttatagtaatgttcgtatgtccaattagataattttatacccaaaccctttacaaatacaaataaatggcaattattttctgtataaaaaactctctttttccctcccggtacgaaatcataaccaataatgtctccattagacataaatataagtttcttattttctgaaatattggaatttatttgcttccaagtacccagcaaacgagtatccatttctttctttgttaaatctggctctttttcttcgcatgaagctaatgaaaacattaaaacagtgcaacaaagtagccaagataatagtcgtaagtatttcatatcaatgtatattatatctgacataagtttcgtcatctgttagaagttcacttggagtgacaaaggagaataccaacttgaaacattttttattcatatcagatttgatttataaagaactttggctataatgccattacaaaggtaatacaaattttattatatcaaaaataatttggacatatttttatttaaggttgttttatatctgattacaacgtgagctcgatataataaggtgtatttatactttagttttagctgacaacaataatatagaaaccaaaaagcgtgtttacccaatggcaaacacgctttttatattgtcagagagttgattactccccctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGUGAGUUUGAUAUAAGAAUUAAAUGAAAAUAAUCCGUUAUUUUUCUUUCUGAUAGAUAAGACAAUCUGAACUAUUUGAAUUCAUUCCAUACAAACUAUACCAUAAAGUUAGCUUAUUUCCAUCUAUUUUAUAGUAAUGUUCGUAUGUCCAAUUAGAUAAUUUUAUACCCAAACCCUUUACAAAUACAAAUAAAUGGCAAUUAUUUUCUGUAUAAAAAACUCUCUUUUUCCCUCCCGGUACGAAAUCAUAACCAAUAAUGUCUCCAUUAGACAUAAAUAUAAGUUUCUUAUUUUCUGAAAUAUUGGAAUUUAUUUGCUUCCAAGUACCCAGCAAACGAGUAUCCAUUUCUUUCUUUGUUAAAUCUGGCUCUUUUUCUUCGCAUGAAGCUAAUGAAAACAUUAAAACAGUGCAACAAAGUAGCCAAGAUAAUAGUCGUAAGUAUUUCAUAUCAAUGUAUAUUAUAUCUGACAUAAGUUUCGUCAUCUGUUAGAAGUUCACUUGGAGUGACAAAGGAGAAUACCAACUUGAAACAUUUUUUAUUCAUAUCAGAUUUGAUUUAUAAAGAACUUUGGCUAUAAUGCCAUUACAAAGGUAAUACAAAUUUUAUUAUAUCAAAAAUAAUUUGGACAUAUUUUUAUUUAAGGUUGUUUUAUAUCUGAUUACAACGUGAGCUCGAUAUAAUAAGGUGUAUUUAUACUUUAGUUUUAGCUGACAACAAUAAUAUAGAAACCAAAAAGCGUGUUUACCCAAUGGCAAACACGCUUUUUAUAUUGUCAGAGAGUUGAUUACUCCCCCUUA ..((((.((((.(((((((((.(((((((((((((.(((((((((((......((((((((((((..((((((......((....))...(((((.(((..(((.(((((((................))))))).)))..))).))))).....)))))).......((((......(((((.((((.(((((...(((.......................((((((((((.((((.(((..(((....((((.....((((((.....))))))..((((((......((((...(((((((((((((((..((((((...........))))))..))).)))..........((((((..(((((((((((......((((.....(((((....))))).....))))...)))).)))).))))))))).....)))))))))....))))....)))))).((((.........))))...))))....)))))).)))).)).)))))))).........((((..........))))..))).))))))))).))))).....((((......)))).......)))).......))))))).)))))))))))))..)))..))))))))))))).....))))))))).))))...)))).(((((.......((((((((....)))))))).......(((((((................(((((((((((((.((....)).)))))))))))))...))))))).)))))............... (-157.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGGGGAGUAAUCAACUCUCUGACAAUAUAAAAAGCGUGUUUGCCAUUGGGUAAACACGCUUUUUGGUUUCUAUAUUAUUGUUGUCAGCUAAAACUAAAGUAUAAAUACACCUUAUUAUAUCGAGCUCACGUUGUAAUCAGAUAUAAAACAACCUUAAAUAAAAAUAUGUCCAAAUUAUUUUUGAUAUAAUAAAAUUUGUAUUACCUUUGUAAUGGCAUUAUAGCCAAAGUUCUUUAUAAAUCAAAUCUGAUAUGAAUAAAAAAUGUUUCAAGUUGGUAUUCUCCUUUGUCACUCCAAGUGAACUUCUAACAGAUGACGAAACUUAUGUCAGAUAUAAUAUACAUUGAUAUGAAAUACUUACGACUAUUAUCUUGGCUACUUUGUUGCACUGUUUUAAUGUUUUCAUUAGCUUCAUGCGAAGAAAAAGAGCCAGAUUUAACAAAGAAAGAAAUGGAUACUCGUUUGCUGGGUACUUGGAAGCAAAUAAAUUCCAAUAUUUCAGAAAAUAAGAAACUUAUAUUUAUGUCUAAUGGAGACAUUAUUGGUUAUGAUUUCGUACCGGGAGGGAAAAAGAGAGUUUUUUAUACAGAAAAUAAUUGCCAUUUAUUUGUAUUUGUAAAGGGUUUGGGUAUAAAAUUAUCUAAUUGGACAUACGAACAUUACUAUAAAAUAGAUGGAAAUAAGCUAACUUUAUGGUAUAGUUUGUAUGGAAUGAAUUCAAAUAGUUCAGAUUGUCUUAUCUAUCAGAAAGAAAAAUAACGGAUUAUUUUCAUUUAAUUCUUAUAUCAAACUCACGUUA ....(((((((.....)))))))((((((((((((((((((((((((....))))))))))))))))).............)))))))......(((.....(((((((((.......((((..((((((..((..((((((((((((((...................((((((.........((((((((......)))))))).((((((..((((......))))((((..((((((..(((((((((((((((.........(((((.....((......))....((((.....))))................))))))))))))))))..........))))))))))..)))).........((((.((((.((((.(((((.((..((((((....))))))...)))))))....))))))))))))...)))))).......))))))((..(((.(((((...((((((.........))))))...))))).)))..)).........)))))))).((((....))))..))))))..))......((....))........))))))..))))....((((((.......)))))))))))))))..((((((((.((((((((((..........(((((((((..(((((((((.(((...........)))..)))))))))..))))))))).((((((....((((((((..(((.((((.((.....))))))..)))...))))))))))))))))))).)))))))))))))..))). (-184.51) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 771 737 169 134 pint:PI1938|5end_hypothetical_protein_1891854..1892084_POSITIVE 305 778 732 180 145 pint:PI2121|5end_glucose_inhibited_division_protein_A_2069889..2070119_POSITIVE 298 786 741 45 5 pint:PI2122|5end_conserved_hypothetical_protein_2069744..2069974_POSITIVE 292 788 741 214 165 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 283 269 222 229 189 pint:PI2074|5end_conserved_hypothetical_protein_2033660..2033890_POSITIVE 279 795 754 149 106 pint:PI0375|5end_hypothetical_protein_345634..345864_POSITIVE 278 371 332 67 33 pint:PI0773|5end_D-3-phosphoglycerate_dehydrogenase_708498..708728_POSITIVE 274 528 483 231 189 pint:PI0684|5end_coproporphyrinogen_III_oxidase_619357..619587_POSITIVE 274 528 483 166 124 pint:PI0683|5end_hypothetical_protein_619292..619522_POSITIVE 273 795 753 113 64 pint:PI1820|5end_hypothetical_protein_1759962..1760192_POSITIVE 268 276 234 97 59 pint:PI0303|5end_hypothetical_protein_286946..287176_POSITIVE 266 425 381 65 24 pint:PI1810|5end_outer_membrane_protein_1748305..1748535_POSITIVE 264 22 1 209 188 pint:PI1819|5end_hypothetical_protein_1756620..1756850_POSITIVE 262 347 306 131 81 pint:PI0432|5end_hypothetical_protein_404781..405011_POSITIVE 262 347 306 74 24 pint:PI0433|5end_hypothetical_protein_404724..404954_POSITIVE 260 779 741 127 88 pint:PI1752|5end_conserved_hypothetical_protein_1680474..1680704_POSITIVE 259 396 363 65 24 pint:PI1348|5end_glycosyltransferase_1262389..1262619_POSITIVE 259 369 321 183 132 pint:PI0560|5end_hypothetical_protein_508600..508830_POSITIVE 257 459 411 98 40 pint:PI0813|5end_conserved_hypothetical_protein_751095..751325_POSITIVE 255 795 751 151 93 pint:PI0005|5end_hypothetical_protein_6984..7214_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 788 741 141 101 pint:PI0258|3end_conjugative_transposon_protein,_TraG_252285..252435_POSITIVE 274 528 483 50 8 pint:PI0681|3end_hypothetical_protein_619256..619406_POSITIVE 270 371 331 150 102 pint:PI0343|3end_hypothetical_protein_317920..318070_POSITIVE 268 276 234 90 52 pint:PI0302|3end_hypothetical_protein_287019..287169_POSITIVE 263 719 674 148 103 pint:PI0127|3end_conserved_hypothetical_protein_120157..120307_POSITIVE 256 42 1 105 66 pint:PI0326|3end_ISPg3-related_transposase_307768..307918_POSITIVE 253 22 2 136 116 pint:PI1019|3end_hypothetical_protein_938412..938562_POSITIVE 251 770 730 125 76 pint:PI0936|3end_conserved_hypothetical_protein_869464..869614_POSITIVE 250 350 302 68 11 pint:PI1120|3end_conserved_hypothetical_protein_1046062..1046212_POSITIVE 249 795 751 111 65 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 249 789 750 97 49 pint:PI1506|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(queuine_synthetase)_1426562..1426712_POSITIVE 249 390 342 147 93 pint:PI0384|3end_probable_radical_SAM_domain_protein_353821..353971_POSITIVE 243 509 468 96 57 pint:PI0536|3end_hypothetical_protein_492623..492773_POSITIVE 241 786 745 126 80 pint:PI2147|3end_GTP-binding_protein_2094407..2094557_POSITIVE 239 20 1 85 66 pint:PI1762|3end_ISPg3-related_transposase_1685645..1685795_POSITIVE 239 20 1 85 66 pint:PI1714|3end_ISPg3-related_transposase_1631352..1631502_POSITIVE 239 20 1 85 66 pint:PI1262|3end_ISPg3-related_transposase,_C-terminal_region_1184322..1184472_POSITIVE 239 20 1 85 66 pint:PI0298|3end_ISPg3-related_transposase_284664..284814_POSITIVE 239 20 1 85 66 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 237 784 745 122 87 pint:PI1818|3end_hypothetical_protein_1757270..1757420_POSITIVE