Origin IGS: tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atatgcaacttatttattattcagattggttttatagttataagatgttgcaaatgtaatactttattttgacattagagcatttacccctcccctatttattaaaatattttaacttttaagttgcgcttttgaagagcgaaagcggcaatacagatttttaaaagcacttttgcactgtgagtatagactatcgcccatttgcttgtatttttattataataatgtaataagtagtatttttctgcgttattatacca Mask Tandem Repeat Region ================================================ tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Find is-nt database================================================ Query_seq: PI1925:PI1926|PI1925:PI1926:conserved hypothetical protein:conserved hypothetical protein:->->:1867994..1868253 260 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1925:PI1926|PI1925:PI1926:conserved hypothetical protein:conserved hypothetical protein:->->:1867994..1868253 260 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1925:PI1926|PI1925:PI1926:conserved hypothetical protein:conserved hypothetical protein:->->:1867994..1868253 260 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 67 End: 156 .................................................................. M V Y T H S A K V L L K I C I A A F A L Q K R N L K V K I F ........................................................................................................ tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1925:PI1926|PI1925:PI1926:conserved hypothetical protein:conserved hypothetical protein:->->:1867994..1868253 260 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Intra-Species Hit: Count: 1 Min: 1 Max: 260 Len: 260 Subject: pint_PI1925_PI1926|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1867994,1868253]|260 HSP 1 e-value: 1.0E-145 bit: 515.0 Len: 260 Query Start:1 Query End:260 Subject Strand: POSITIVE Subject Start: 1 Subject End: 260 tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat tggtataataacgcagaaaaatactacttattacattattataataaaaatacaagcaaatgggcgatagtctatactcacagtgcaaaagtgcttttaaaaatctgtattgccgctttcgctcttcaaaagcgcaacttaaaagttaaaatattttaataaataggggaggggtaaatgctctaatgtcaaaataaagtattacatttgcaacatcttataactataaaaccaatctgaataataaataagttgcatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUAUAAUAACGCAGAAAAAUACUACUUAUUACAUUAUUAUAAUAAAAAUACAAGCAAAUGGGCGAUAGUCUAUACUCACAGUGCAAAAGUGCUUUUAAAAAUCUGUAUUGCCGCUUUCGCUCUUCAAAAGCGCAACUUAAAAGUUAAAAUAUUUUAAUAAAUAGGGGAGGGGUAAAUGCUCUAAUGUCAAAAUAAAGUAUUACAUUUGCAACAUCUUAUAACUAUAAAACCAAUCUGAAUAAUAAAUAAGUUGCAUAU ((((........(((((..((((((..(((((((((((..........((((((.....((((((....))))))......((..(....)..))..........))))))...((.((..((((((........((((....))))....(((((....)))))...))))))..)).))..))))))...)))))))))))...)))))......((((....)))).)))).......................... (-39.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUGCAACUUAUUUAUUAUUCAGAUUGGUUUUAUAGUUAUAAGAUGUUGCAAAUGUAAUACUUUAUUUUGACAUUAGAGCAUUUACCCCUCCCCUAUUUAUUAAAAUAUUUUAACUUUUAAGUUGCGCUUUUGAAGAGCGAAAGCGGCAAUACAGAUUUUUAAAAGCACUUUUGCACUGUGAGUAUAGACUAUCGCCCAUUUGCUUGUAUUUUUAUUAUAAUAAUGUAAUAAGUAGUAUUUUUCUGCGUUAUUAUACCA ....((((((((................((((((..((((.(((.((((((....))))))))).....))))..))))))..............(((((....)))))..........)))))))).........(((((((.(.(((.................(((....))).((((....))))......)))).)))))))..........((((((((((((..(((......)))..))))))))))))... (-46.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 136 112 78 54 pint:PI0568|5end_hypothetical_protein_512950..513180_POSITIVE 309 136 112 78 54 pint:PI0089|5end_hypothetical_protein_88162..88392_POSITIVE 301 149 102 86 48 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 300 136 93 205 161 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 299 136 112 97 73 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 299 136 112 51 27 pint:PI2023|5end_hypothetical_protein_1978585..1978815_POSITIVE 299 136 112 39 15 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 299 136 112 224 200 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 299 136 112 137 113 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 299 136 112 54 30 pint:PI0866|5end_hypothetical_protein_803776..804006_POSITIVE 299 136 112 83 59 pint:PI0659|5end_hypothetical_protein_603203..603433_POSITIVE 298 136 112 230 206 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 274 137 113 25 1 pint:PI0970|5end_hypothetical_protein_897254..897484_POSITIVE 268 136 112 45 21 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 264 86 54 129 97 pint:PI0344|5end_conserved_hypothetical_protein_318178..318408_POSITIVE 260 139 103 162 123 pint:PI1285|5end_ATP_synthase,_B_subunit_1200348..1200578_POSITIVE 260 136 92 56 1 pint:PI0038|5end_transcriptional_regulator,_LysR_family_39578..39808_POSITIVE 258 89 44 155 99 pint:PI0898|5end_conserved_hypothetical_protein_828474..828704_POSITIVE 256 126 81 216 162 pint:PI1332|5end_aminotransferase,_possible_pyridoxal-phosphate-dependent_aminotransferase_1249374..1249604_POSITIVE 256 125 82 189 148 pint:PI0975|5end_glycine_dehydrogenase_[decarboxylating]_subunit_2_900798..901028_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 136 112 103 79 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 301 149 102 77 39 pint:PI2016|3end_ribosomal_large_subunit_pseudouridine_synthase_D_1974933..1975083_POSITIVE 297 137 93 131 85 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 270 126 81 129 68 pint:PI0639|3end_small_heat_shock_protein_583165..583315_POSITIVE 265 122 83 138 93 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 265 122 83 47 2 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 260 139 103 57 18 pint:PI1284|3end_ATP_synthase,_delta_subunit_1200323..1200473_POSITIVE 258 89 44 138 82 pint:PI0897|3end_ferredoxin,_4Fe-4S_828537..828687_POSITIVE 256 125 82 136 95 pint:PI0973|3end_glycine_dehydrogenase_[decarboxylating]_subunit_1_900825..900975_POSITIVE 255 140 108 151 98 pint:PI0767|3end_possible_TonB-dependent_receptor_704025..704175_POSITIVE 254 97 54 53 15 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 252 96 60 126 91 pint:PI1986|3end_hypothetical_protein_1941551..1941701_POSITIVE 251 123 82 114 64 pint:PI0369|3end_hypothetical_protein_340409..340559_POSITIVE 251 127 102 105 77 pint:PI0026|3end_two-component_system_response_regulator_30537..30687_POSITIVE 248 124 84 79 36 pint:PI1861|3end_hypothetical_protein_1806114..1806264_POSITIVE 248 140 104 149 114 pint:PI0700|3end_UDP-glucose_4-epimerase_638413..638563_POSITIVE 243 123 81 114 68 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 242 97 56 111 64 pint:PI1818|3end_hypothetical_protein_1757270..1757420_POSITIVE 239 127 84 102 41 pint:PI1768|3end_conserved_hypothetical_protein_1696639..1696789_POSITIVE 237 135 111 151 131 pint:PI0750|3end_zinc_protease_680633..680783_POSITIVE