Origin IGS: cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttataagttagattaaaaattattgttcactaatcgtacaaaggtaattatttttcggtaattattcatcttttttcttataattctgtttgtactctgttaaatataaaaaaggctcccttaatttggtactttcttcgtgtttttcagccatttattgtggaataattccccttttgtagtctacaagaaatatactgaaatggctttcgttttgtaaagataatttcg Mask Tandem Repeat Region ================================================ cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Find is-nt database================================================ Query_seq: PI1905:PI1906|PI1905:PI1906:hypothetical protein:zinc protease, immunoreactive 106 kDa antigen PG115:->->:1847915..1848147 233 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1905:PI1906|PI1905:PI1906:hypothetical protein:zinc protease, immunoreactive 106 kDa antigen PG115:->->:1847915..1848147 233 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1905:PI1906|PI1905:PI1906:hypothetical protein:zinc protease, immunoreactive 106 kDa antigen PG115:->->:1847915..1848147 233 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 78 End: 167 ............................................................................. M A E K H E E S T K L R E P F L Y L T E Y K Q N Y K K K D E .................................................................. Protein_Len: 35 Strand: - Start: 67 End: 171 .................................................................. E V I F P Q F V R L F Y W I L P A K K I N L L T C V S N Y S F L H I M .............................................................. Protein_Len: 30 Strand: - Start: 53 End: 142 .................................................... L L P I I G C Y I A S F C S S L V L N L S G K K Y K V S Y M ........................................................................................... cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1905:PI1906|PI1905:PI1906:hypothetical protein:zinc protease, immunoreactive 106 kDa antigen PG115:->->:1847915..1848147 233 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Intra-Species Hit: Count: 1 Min: 1 Max: 233 Len: 233 Subject: pint_PI1905_PI1906|hypothetical protein:zinc protease, immunoreactive 106 kDa antigen PG115|POSITIVE:POSITIVE|[1847915,1848147]|233 HSP 1 e-value: 1.0E-129 bit: 462.0 Len: 233 Query Start:1 Query End:233 Subject Strand: POSITIVE Subject Start: 1 Subject End: 233 cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa cgaaattatctttacaaaacgaaagccatttcagtatatttcttgtagactacaaaaggggaattattccacaataaatggctgaaaaacacgaagaaagtaccaaattaagggagccttttttatatttaacagagtacaaacagaattataagaaaaaagatgaataattaccgaaaaataattacctttgtacgattagtgaacaataatttttaatctaacttataaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAAAUUAUCUUUACAAAACGAAAGCCAUUUCAGUAUAUUUCUUGUAGACUACAAAAGGGGAAUUAUUCCACAAUAAAUGGCUGAAAAACACGAAGAAAGUACCAAAUUAAGGGAGCCUUUUUUAUAUUUAACAGAGUACAAACAGAAUUAUAAGAAAAAAGAUGAAUAAUUACCGAAAAAUAAUUACCUUUGUACGAUUAGUGAACAAUAAUUUUUAAUCUAACUUAUAAAA .((((((((.(((((........((((((((.(((.(((.....))).))).......((((....)))).....)))))))).........((((((.((.((........)).)).))))))............(((((((...((((((.........(((.....)))........))))))...))))))).....)))))..))))))))................. (-31.53) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUAAGUUAGAUUAAAAAUUAUUGUUCACUAAUCGUACAAAGGUAAUUAUUUUUCGGUAAUUAUUCAUCUUUUUUCUUAUAAUUCUGUUUGUACUCUGUUAAAUAUAAAAAAGGCUCCCUUAAUUUGGUACUUUCUUCGUGUUUUUCAGCCAUUUAUUGUGGAAUAAUUCCCCUUUUGUAGUCUACAAGAAAUAUACUGAAAUGGCUUUCGUUUUGUAAAGAUAAUUUCG ..(((((((..((((.((.((((((((.....((((..(((....))))))).....)))))))).)).)))).....)))))))...(((((.(((...(..(((((..((.((((..((........))..)))).))..)))))..).(((((((.(((((((((((........))))..)))))))...........))))))).........))).)))))...... (-35.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 50 1 193 153 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 261 63 23 66 20 pint:PI0993|5end_probable_TPR-repeat-containing_protein_917175..917405_POSITIVE 258 48 4 141 97 pint:PI0089|5end_hypothetical_protein_88162..88392_POSITIVE 247 98 53 229 185 pint:PI1797|5end_hypothetical_protein_1731147..1731377_POSITIVE 247 103 61 73 31 pint:PI0835|5end_conserved_hypothetical_protein;_possible_protein-export_membrane_protein_771405..771635_POSITIVE 244 70 22 55 2 pint:PI0546|5end_hypothetical_protein_497799..498029_POSITIVE 242 99 53 207 154 pint:PI1070|5end_peptidoglycan-associated_lipoprotein_986110..986340_POSITIVE 236 119 74 111 69 pint:PI2172|5end_hypothetical_protein_2113819..2114049_POSITIVE 236 119 74 87 45 pint:PI2173|5end_hypothetical_protein_2113795..2114025_POSITIVE 230 120 71 67 15 pint:PI1400|5end_hypothetical_protein_1321391..1321621_POSITIVE 230 211 175 176 137 pint:PI0912|5end_conserved_hypothetical_protein_842034..842264_POSITIVE 230 70 23 147 98 pint:PI0382|5end_conserved_hypothetical_protein_351510..351740_POSITIVE 224 45 4 98 56 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 222 117 82 85 48 pint:PI0919|5end_conserved_hypothetical_protein_849298..849528_POSITIVE 219 128 81 199 148 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 217 117 80 62 28 pint:PI1736|5end_topoisomerase_IV,_subunit_A_1654254..1654484_POSITIVE 213 139 91 93 23 pint:PI0980|5end_conserved_hypothetical_protein_905703..905933_POSITIVE 210 100 51 103 52 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 208 120 80 136 105 pint:PI0750|5end_zinc_protease_677746..677976_POSITIVE 204 118 87 59 23 pint:PI1007|5end_conserved_hypothetical_protein_926956..927186_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 98 53 88 44 pint:PI1796|3end_2-hydroxyhepta-2,4-diene-1,7-dioate_isomerase,_fumarylacetoacetate_hydrolase_family_protein_1731086..1731236_POSITIVE 247 103 61 61 19 pint:PI0834|3end_conserved_hypothetical_protein_771473..771623_POSITIVE 240 115 76 91 49 pint:PI0576|3end_helicase,_UvrD/Rep_521724..521874_POSITIVE 236 119 74 43 1 pint:PI2171|3end_conserved_hypothetical_protein_2113831..2113981_POSITIVE 230 70 23 111 62 pint:PI0381|3end_probable_transcriptional_regulator,_AraC_family_351554..351704_POSITIVE 225 85 42 98 48 pint:PI1909|3end_DNA_gyrase,_B_subunit_1855270..1855420_POSITIVE 223 96 56 107 63 pint:PI0135|3end_internalin-related_protein_127257..127407_POSITIVE 219 128 81 70 19 pint:PI0814|3end_conserved_hypothetical_protein_754426..754576_POSITIVE 217 174 134 102 61 pint:PI1911|3end_ribosomal_protein_S20_1856235..1856385_POSITIVE 217 117 80 51 17 pint:PI1735|3end_conserved_hypothetical_protein_1654323..1654473_POSITIVE 217 118 72 135 95 pint:PI1672|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1587529..1587679_POSITIVE 211 70 28 43 2 pint:PI0545|3end_sugar_kinase_497867..498017_POSITIVE 206 27 1 88 63 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 204 118 87 72 36 pint:PI1006|3end_conserved_hypothetical_protein_927049..927199_POSITIVE 202 119 78 66 24 pint:PI0183|3end_hypothetical_protein_174810..174960_POSITIVE 200 66 21 86 35 pint:PI1099|3end_low_molecular_weight_protein-tyrosine-phosphatase_1023636..1023786_POSITIVE 198 61 21 148 114 pint:PI1275|3end_indole-3-glycerol_phosphate_synthase_1194307..1194457_POSITIVE 197 73 49 109 84 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 196 117 72 76 21 pint:PI1613|3end_hypothetical_protein_1540906..1541056_POSITIVE 195 36 2 74 39 pint:PI1203|3end_conserved_hypothetical_protein_1124439..1124589_POSITIVE