Origin IGS: agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtaagacacaggaaaaacgccgcagaagtgtaaatattttttcagaaaacatctatcacttaacttgctctatatcagcacattataaaacctacgctttcgcaacgtcaaatcgaaattatcatttttctatcgaattatctttacaaaactgaagcgattttcagcacttgccacaagaaaggaaacgagaagcaattgcctattgaaaaattactgaactgcttttgttgaaaacagaagataacaaactgaaatacagtggctataccacgacgaaactacatttgtgatatttcacatttttaagtggttaattttacgacaaaacaaaaaaaatgattaactttgtaggttgataaatagagact Mask Tandem Repeat Region ================================================ agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Find is-nt database================================================ Query_seq: PI1889:PI1890|PI1889:PI1890:UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase:cell division protein FtsW:->->:1832652..1833025 374 agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1889:PI1890|PI1889:PI1890:UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase:cell division protein FtsW:->->:1832652..1833025 374 agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1889:PI1890|PI1889:PI1890:UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase:cell division protein FtsW:->->:1832652..1833025 374 agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 103 End: 225 ...................................................................................................... M A T V F Q F V I F C F Q Q K Q F S N F S I G N C F S F P F L W Q V L K I A S V L ..................................................................................................................................................... Protein_Len: 39 Strand: - Start: 220 End: 336 ........................................................................................................................................................................................................................... N Q L S L E I S F H Y N R N S T A F A Y T K Y H A S I S C T L H Y I N E S F M ...................................... Protein_Len: 50 Strand: - Start: 141 End: 290 ............................................................................................................................................ C F C N L L K E I P L Q K E N G K K H C T S F I A E T K Y L Y N S L F I I I E I Q R Q S L T P K I M .................................................................................... agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -5.3 Tail_Score: -4.7078 Start: 70 End: 83 Full_Region: cgacgaaactacatt tgtga tatt tcaca tttttaagtggttaa .....................................................................tgtgatatttcaca................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 60 HP_score: -3.3 Tail_Score: -4.3898 Start: 205 End: 220 Full_Region: TTCTTGTGGCAAGTG CTGAA AATCGC TTCAG TTTTGTAAAGATAAT ............................................................................................................................................................................................................CTGAAAATCGCTTCAG.......................................................................................................................................................... Find igs database================================================ Query_seq: PI1889:PI1890|PI1889:PI1890:UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase:cell division protein FtsW:->->:1832652..1833025 374 agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Intra-Species Hit: Count: 1 Min: 1 Max: 374 Len: 374 Subject: pint_PI1889_PI1890|UDP-N-acetylglucosamine--N-acetylmuramyl- (pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase:cell division protein FtsW|POSITIVE:POSITIVE|[1832652,1833025]|374 HSP 1 e-value: 0.0 bit: 694.0 Len: 374 Query Start:1 Query End:374 Subject Strand: POSITIVE Subject Start: 1 Subject End: 374 agtctctatttatcaacctacaaagttaatcannnnnnnngttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta agtctctatttatcaacctacaaagttaatcattttttttgttttgtcgtaaaattaaccacttaaaaatgtgaaatatcacaaatgtagtttcgtcgtggtatagccactgtatttcagtttgttatcttctgttttcaacaaaagcagttcagtaatttttcaataggcaattgcttctcgtttcctttcttgtggcaagtgctgaaaatcgcttcagttttgtaaagataattcgatagaaaaatgataatttcgatttgacgttgcgaaagcgtaggttttataatgtgctgatatagagcaagttaagtgatagatgttttctgaaaaaatatttacacttctgcggcgtttttcctgtgtcttactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUCUCUAUUUAUCAACCUACAAAGUUAAUCAUUUUUUUUGUUUUGUCGUAAAAUUAACCACUUAAAAAUGUGAAAUAUCACAAAUGUAGUUUCGUCGUGGUAUAGCCACUGUAUUUCAGUUUGUUAUCUUCUGUUUUCAACAAAAGCAGUUCAGUAAUUUUUCAAUAGGCAAUUGCUUCUCGUUUCCUUUCUUGUGGCAAGUGCUGAAAAUCGCUUCAGUUUUGUAAAGAUAAUUCGAUAGAAAAAUGAUAAUUUCGAUUUGACGUUGCGAAAGCGUAGGUUUUAUAAUGUGCUGAUAUAGAGCAAGUUAAGUGAUAGAUGUUUUCUGAAAAAAUAUUUACACUUCUGCGGCGUUUUUCCUGUGUCUUACUUA ..............(((((((.((((((.((((((((((((..(((((.........(((((.......(((((....))))).(((......))).)))))...(((((.........(...(((((...(((((((.....)))))))....)))))....)...((((....))))...............)))))....((((((......))))))........)))))..)))..)))))))))))))))............((....))))))))).......(((..((((((((((..((.(((((.((((((((((.....)))))))))))))))..))...)))....))))))).)))... (-76.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUAAGACACAGGAAAAACGCCGCAGAAGUGUAAAUAUUUUUUCAGAAAACAUCUAUCACUUAACUUGCUCUAUAUCAGCACAUUAUAAAACCUACGCUUUCGCAACGUCAAAUCGAAAUUAUCAUUUUUCUAUCGAAUUAUCUUUACAAAACUGAAGCGAUUUUCAGCACUUGCCACAAGAAAGGAAACGAGAAGCAAUUGCCUAUUGAAAAAUUACUGAACUGCUUUUGUUGAAAACAGAAGAUAACAAACUGAAAUACAGUGGCUAUACCACGACGAAACUACAUUUGUGAUAUUUCACAUUUUUAAGUGGUUAAUUUUACGACAAAACAAAAAAAAUGAUUAACUUUGUAGGUUGAUAAAUAGAGACU .......(((...(......)...((..(((((((...........(((.....)))......(((..((((.......))))..))).......)))))))..))...)))...((....((((((........(((..((((((((....(((.((((((...(((((..((((...))))........(((...((....))...)))........))))).)))))).))).......))))))))..............((((.....))))..)))..(((((..(((((....))))).........((((((((.....................)))))))).)))))..))))))...)).... (-61.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 582 277 231 214 168 pint:PI0407|5end_tryptophanyl-tRNA_synthetase_(tryptophan--tRNA_ligase)_372973..373203_POSITIVE 523 290 243 221 173 pint:PI1949|5end_aminotransferase,_class_V_1903218..1903448_POSITIVE 523 290 243 87 39 pint:PI1948|5end_hypothetical_protein_1903084..1903314_POSITIVE 509 285 241 165 121 pint:PI1870|5end_hypothetical_protein_1815302..1815532_POSITIVE 452 239 192 210 161 pint:PI1734|5end_possible_carboxyl-terminal_protease-related_protein_1652409..1652639_POSITIVE 452 285 245 41 1 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 448 277 236 209 171 pint:PI1830|5end_conserved_hypothetical_protein_1769439..1769669_POSITIVE 447 285 242 187 144 pint:PI0842|5end_5-formyltetrahydrofolate_cyclo-ligase_775533..775763_POSITIVE 447 285 242 60 17 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 443 319 272 105 57 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 438 285 251 38 7 pint:PI1679|5end_hypothetical_protein_1594744..1594974_POSITIVE 427 237 191 165 120 pint:PI0202|5end_DNA_helicase_191753..191983_POSITIVE 420 277 231 151 105 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 415 291 251 47 7 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 410 285 243 228 185 pint:PI0552|5end_hypothetical_protein_502716..502946_POSITIVE 408 277 231 156 110 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 402 277 231 167 121 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 400 239 197 210 166 pint:PI1392|5end_hypothetical_protein_1311901..1312131_POSITIVE 396 257 213 231 187 pint:PI0183|5end_hypothetical_protein_174347..174577_POSITIVE 389 285 241 77 34 pint:PI0978|5end_hypothetical_protein_904867..905097_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 558 277 231 101 55 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 553 285 242 115 72 pint:PI2131|3end_hypothetical_protein_2080868..2081018_POSITIVE 540 277 231 147 101 pint:PI1167|3end_hypothetical_protein_1089890..1090040_POSITIVE 519 277 231 107 61 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE 519 277 231 107 61 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 515 277 231 128 82 pint:PI1905|3end_hypothetical_protein_1847854..1848004_POSITIVE 485 277 231 107 61 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 466 286 243 124 80 pint:PI1666|3end_conserved_hypothetical_protein_1579418..1579568_POSITIVE 464 277 231 107 61 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 455 277 231 147 101 pint:PI0104|3end_hypothetical_protein_99346..99496_POSITIVE 448 277 236 129 91 pint:PI1829|3end_hypothetical_protein_1769439..1769589_POSITIVE 442 286 243 130 86 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 440 269 221 151 103 pint:PI1129|3end_hypothetical_protein_1055603..1055753_POSITIVE 427 269 221 151 103 pint:PI1346|3end_hypothetical_protein_1261028..1261178_POSITIVE 427 237 191 67 22 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE 426 230 186 147 98 pint:PI1733|3end_hypothetical_protein_1652437..1652587_POSITIVE 422 277 231 111 65 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 422 277 231 67 21 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 420 260 214 90 43 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 410 280 231 150 101 pint:PI0841|3end_hypothetical_protein_775581..775731_POSITIVE