Origin IGS: taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtattgataaagagatagttagaagaaagatatgtttgtgaaaaatatttacatctttctaagctttccctcctatatataataaggtatctaagcagagtagggtagttgcgaaaacgtttcttttatgtagacggcttgcattgcgagggttgttcttcatttcttcctttcttccttttta Mask Tandem Repeat Region ================================================ taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Find is-nt database================================================ Query_seq: PI1865:PI1866|PI1865:PI1866:chromate transport protein:hypothetical protein:->->:1811648..1811832 185 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1865:PI1866|PI1865:PI1866:chromate transport protein:hypothetical protein:->->:1811648..1811832 185 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1865:PI1866|PI1865:PI1866:chromate transport protein:hypothetical protein:->->:1811648..1811832 185 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 22 End: 123 ..................... M K N N P R N A S R L H K R N V F A T T L L C L D T L L Y I G G K A .............................................................. Protein_Len: 33 Strand: + Start: 87 End: 185 ...................................................................................... M L R Y L I I Y R R E S L E R C K Y F S Q T Y L S S N Y L F I N T Protein_Len: 36 Strand: - Start: 3 End: 110 .. F P L F S S I F F L G R L A L R R C L L F T K A V V R S Q K S V K N Y M ........................................................................... taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1865:PI1866|PI1865:PI1866:chromate transport protein:hypothetical protein:->->:1811648..1811832 185 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Intra-Species Hit: Count: 1 Min: 1 Max: 185 Len: 185 Subject: pint_PI1865_PI1866|chromate transport protein:hypothetical protein|POSITIVE:POSITIVE|[1811648,1811832]|185 HSP 1 e-value: 1.0E-101 bit: 367.0 Len: 185 Query Start:1 Query End:185 Subject Strand: POSITIVE Subject Start: 1 Subject End: 185 taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca taaaaaggaagaaaggaagaaatgaagaacaaccctcgcaatgcaagccgtctacataaaagaaacgttttcgcaactaccctactctgcttagataccttattatatataggagggaaagcttagaaagatgtaaatatttttcacaaacatatctttcttctaactatctctttatcaataca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAGGAAGAAAGGAAGAAAUGAAGAACAACCCUCGCAAUGCAAGCCGUCUACAUAAAAGAAACGUUUUCGCAACUACCCUACUCUGCUUAGAUACCUUAUUAUAUAUAGGAGGGAAAGCUUAGAAAGAUGUAAAUAUUUUUCACAAACAUAUCUUUCUUCUAACUAUCUCUUUAUCAAUACA .....((..((((.((((((.(((........(((((....(((((((..(((.......)))...))))..))).(((..(......)..)))..................)))))........((((((((....)))))))).....))).))))))))))..))................. (-31.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUAUUGAUAAAGAGAUAGUUAGAAGAAAGAUAUGUUUGUGAAAAAUAUUUACAUCUUUCUAAGCUUUCCCUCCUAUAUAUAAUAAGGUAUCUAAGCAGAGUAGGGUAGUUGCGAAAACGUUUCUUUUAUGUAGACGGCUUGCAUUGCGAGGGUUGUUCUUCAUUUCUUCCUUUCUUCCUUUUUA ........(((((((.........((((((((.((..(((.....)))..)).))))))))..((((.....(((..........))).....)))).(((.((((.((.(((((...((((((......).)))))..)))))....(((((....)))))....)).)))).))).))))))) (-35.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 401 180 132 188 140 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 391 158 115 195 149 pint:PI0038|5end_transcriptional_regulator,_LysR_family_39578..39808_POSITIVE 346 89 49 146 107 pint:PI0886|5end_hypothetical_protein_817640..817870_POSITIVE 346 89 49 42 3 pint:PI0887|5end_hypothetical_protein_817536..817766_POSITIVE 344 154 112 92 51 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 340 180 132 138 90 pint:PI0653|5end_hypothetical_protein_596046..596276_POSITIVE 331 157 111 153 105 pint:PI0949|5end_endonuclease_882264..882494_POSITIVE 313 59 13 123 83 pint:PI0720|5end_conserved_hypothetical_protein_653949..654179_POSITIVE 313 180 131 212 163 pint:PI0660|5end_conserved_hypothetical_protein_603438..603668_POSITIVE 311 50 2 125 68 pint:PI1867|5end_helicase_related_protein_1812390..1812620_POSITIVE 309 91 51 143 105 pint:PI0489|5end_probable_cardiolipin_synthetase_447610..447840_POSITIVE 303 154 112 229 188 pint:PI1206|5end_ATP-dependent_DNA_helicase,_UvrD/PcrA/Rep_family_1124698..1124928_POSITIVE 301 69 26 103 48 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 299 149 103 216 172 pint:PI1456|5end_conserved_hypothetical_protein_1378864..1379094_POSITIVE 299 160 111 126 75 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 298 180 132 53 5 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 296 123 85 153 113 pint:PI1236|5end_10_kDa_chaperonin,_cpn10,_GroES_1158934..1159164_POSITIVE 295 69 26 103 48 pint:PI0349|5end_hypothetical_protein_323144..323374_POSITIVE 293 180 132 124 76 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 292 149 106 93 49 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 401 180 132 73 25 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 382 155 115 148 107 pint:PI0037|3end_hypothetical_protein_39616..39766_POSITIVE 346 89 49 95 56 pint:PI0884|3end_hypothetical_protein_817669..817819_POSITIVE 313 59 13 104 64 pint:PI0719|3end_conserved_hypothetical_protein_654010..654160_POSITIVE 313 180 131 90 41 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 312 180 132 112 64 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 303 154 112 86 45 pint:PI1204|3end_hypothetical_protein_1124635..1124785_POSITIVE 301 69 26 90 35 pint:PI1642|3end_conserved_hypothetical_protein_1560439..1560589_POSITIVE 295 69 26 90 35 pint:PI0348|3end_conserved_hypothetical_protein_323211..323361_POSITIVE 290 157 111 62 15 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 290 55 23 119 89 pint:PI0734|3end_ABC_transporter,_ATP-binding_/_permease_668760..668910_POSITIVE 278 49 5 135 85 pint:PI0640|3end_conserved_hypothetical_protein_583821..583971_POSITIVE 277 180 132 112 64 pint:PI1308|3end_hypothetical_protein_1223717..1223867_POSITIVE 268 54 21 58 9 pint:PI0589|3end_asparaginyl-tRNA_synthetase_(asparagine--tRNA_ligase)_536959..537109_POSITIVE 268 180 132 112 64 pint:PI0069|3end_hypothetical_protein_67379..67529_POSITIVE 267 120 73 97 46 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 266 180 132 85 37 pint:PI1005|3end_hypothetical_protein_926481..926631_POSITIVE 264 83 48 37 1 pint:PI1590|3end_transcriptional_regulator_1518498..1518648_POSITIVE 262 77 42 73 39 pint:PI1466|3end_GTP-binding_protein,_possible_GTP1/OBG_family_1393133..1393283_POSITIVE 261 49 6 122 61 pint:PI0936|3end_conserved_hypothetical_protein_869464..869614_POSITIVE