Origin IGS: actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gccataaatacctaatccaattcctcccactcgtgcgccgataaatatcatagccaaaaccacgagtaattctgcaattactataagttccattatactgttttcgtttgttttcgtattgtattaatgcaaaaataagatttttatactatggttggtgcttttattgacaaaaacatccttcttgttattatcttttaacaaaattgtttaaatgttggtcaaaaacgctaccaaaatactataaaaaatgttgtataggttatcaaaagatatcaaaacatataagaacaaatcgtttgtaaattacaacttaatttccgaagccataatactgactctttaattttatatagattatattcgagaaatggaataaatacaaaaataatgactaactttgcaacagtagtaattcaataaaacaatttaaacacattaaagt Mask Tandem Repeat Region ================================================ actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Find is-nt database================================================ Query_seq: PI1833:PI1834|PI1833:PI1834:bacterioferritin comigratory protein:anaerobic C4-dicarboxylate membrane transporter:->->:1774556..1775000 445 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1833:PI1834|PI1833:PI1834:bacterioferritin comigratory protein:anaerobic C4-dicarboxylate membrane transporter:->->:1774556..1775000 445 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1833:PI1834|PI1833:PI1834:bacterioferritin comigratory protein:anaerobic C4-dicarboxylate membrane transporter:->->:1774556..1775000 445 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 30 End: 167 ............................. M T T V A K L V I I F V F I P F L E Y N L Y K I K E S V L W L R K L S C N L Q T I C S Y M F ...................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: + Start: 310 End: 444 ..................................................................................................................................................................................................................................................................................................................... M F A L I Q Y E N K R K Q Y N G T Y S N C R I T R G F G Y D I Y R R T S G R N W I R Y L W . Protein_Len: 32 Strand: + Start: 350 End: 445 ............................................................................................................................................................................................................................................................................................................................................................. M M E L I V I A E L L V V L A M I F I G A R V G G I G L G I Y G Protein_Len: 39 Strand: - Start: 322 End: 438 ................................................................................................................................................................................................................................................................................................................................. Y L V F V F S F L I I S S I T I A S N S T T K A I I N I P A R T P P I P N P M ....... Protein_Len: 32 Strand: - Start: 144 End: 239 ............................................................................................................................................... L R N T R I H K S I K Q Y G I C C K K Y Y K P L T K S W C K F M .............................................................................................................................................................................................................. Protein_Len: 31 Strand: - Start: 103 End: 195 ...................................................................................................... L T L I I A E S I L N Y N V F S K N K Y T K I D K S L R Y L M .......................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: - Start: 26 End: 151 ......................... Q I V V T A F N T M I K T N I G N R S Y L R Y L I L S D T N H S R F N L Q L K C V M ...................................................................................................................................................................................................................................................................................................... actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 176 End: 205 ...............................................................................................................................................................................TTTGATAACCTATACAACATTTTTTATAGT................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 60 End: 89 ...........................................................TTTGTATTTATTCCATTTCTCGAATATAAT.................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1833:PI1834|PI1833:PI1834:bacterioferritin comigratory protein:anaerobic C4-dicarboxylate membrane transporter:->->:1774556..1775000 445 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Intra-Species Hit: Count: 1 Min: 1 Max: 445 Len: 445 Subject: pint_PI1833_PI1834|bacterioferritin comigratory protein:anaerobic C4-dicarboxylate membrane transporter|POSITIVE:POSITIVE|[1774556,1775000]|445 HSP 1 e-value: 0.0 bit: 882.0 Len: 445 Query Start:1 Query End:445 Subject Strand: POSITIVE Subject Start: 1 Subject End: 445 actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc actttaatgtgtttaaattgttttattgaattactactgttgcaaagttagtcattatttttgtatttattccatttctcgaatataatctatataaaattaaagagtcagtattatggcttcggaaattaagttgtaatttacaaacgatttgttcttatatgttttgatatcttttgataacctatacaacattttttatagtattttggtagcgtttttgaccaacatttaaacaattttgttaaaagataataacaagaaggatgtttttgtcaataaaagcaccaaccatagtataaaaatcttatttttgcattaatacaatacgaaaacaaacgaaaacagtataatggaacttatagtaattgcagaattactcgtggttttggctatgatatttatcggcgcacgagtgggaggaattggattaggtatttatggc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUUAAUGUGUUUAAAUUGUUUUAUUGAAUUACUACUGUUGCAAAGUUAGUCAUUAUUUUUGUAUUUAUUCCAUUUCUCGAAUAUAAUCUAUAUAAAAUUAAAGAGUCAGUAUUAUGGCUUCGGAAAUUAAGUUGUAAUUUACAAACGAUUUGUUCUUAUAUGUUUUGAUAUCUUUUGAUAACCUAUACAACAUUUUUUAUAGUAUUUUGGUAGCGUUUUUGACCAACAUUUAAACAAUUUUGUUAAAAGAUAAUAACAAGAAGGAUGUUUUUGUCAAUAAAAGCACCAACCAUAGUAUAAAAAUCUUAUUUUUGCAUUAAUACAAUACGAAAACAAACGAAAACAGUAUAAUGGAACUUAUAGUAAUUGCAGAAUUACUCGUGGUUUUGGCUAUGAUAUUUAUCGGCGCACGAGUGGGAGGAAUUGGAUUAGGUAUUUAUGGC ....(((((...((((.((((..((.((......)).))..))))..)))).))))).....(((((((.((((((((((........................((...((((((((((((...(((((((....(((((((.(((((.((..((((((((((.(((((.((((....)))).............((((((((.(((.(((((.((....(((((.(((((((......((((((((........)))))))).)))))))...))))).....)))))))..))).)))))))).....((((((.(((.....))).))))))....)))))...)))))..)))))..)).))..)))..)))))))...)))))))))))))))))))..))..(((....))).)))))).)))).)))))))....... (-80.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCAUAAAUACCUAAUCCAAUUCCUCCCACUCGUGCGCCGAUAAAUAUCAUAGCCAAAACCACGAGUAAUUCUGCAAUUACUAUAAGUUCCAUUAUACUGUUUUCGUUUGUUUUCGUAUUGUAUUAAUGCAAAAAUAAGAUUUUUAUACUAUGGUUGGUGCUUUUAUUGACAAAAACAUCCUUCUUGUUAUUAUCUUUUAACAAAAUUGUUUAAAUGUUGGUCAAAAACGCUACCAAAAUACUAUAAAAAAUGUUGUAUAGGUUAUCAAAAGAUAUCAAAACAUAUAAGAACAAAUCGUUUGUAAAUUACAACUUAAUUUCCGAAGCCAUAAUACUGACUCUUUAAUUUUAUAUAGAUUAUAUUCGAGAAAUGGAAUAAAUACAAAAAUAAUGACUAACUUUGCAACAGUAGUAAUUCAAUAAAACAAUUUAAACACAUUAAAGU ....(((((...................(((((((.((.(((....)))...))......))))))).....((.(((((((((......................((((((((.((((((...)))))).)))))))).((((((((.(((..(((((((.(((.(((((...(((((.....((((((........))))))..........))))).)))))))).)).))))).))).))))))))..((((((.(((((((((..............((((((((........((((.((((((.....)))))).))))..((......)).........))))))))......(((((.(....).))))).............))).)))))))))))).)))))))))))........)))))............. (-68.36) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 419 371 204 150 pint:PI0784|5end_possible_amidase_enhancer_precursor_721892..722122_POSITIVE 338 418 371 104 55 pint:PI1333|5end_conserved_hypothetical_protein_1250777..1251007_POSITIVE 317 436 391 190 137 pint:PI0222|5end_conserved_hypothetical_protein_217808..218038_POSITIVE 311 436 391 186 137 pint:PI1056|5end_cell-division_ATP-binding_protein_976247..976477_POSITIVE 306 414 371 227 177 pint:PI0711|5end_ISPg3-related_transposase_647934..648164_POSITIVE 305 432 391 214 154 pint:PI0277|5end_hypothetical_protein_264980..265210_POSITIVE 302 418 371 224 173 pint:PI0382|5end_conserved_hypothetical_protein_351510..351740_POSITIVE 300 436 393 88 40 pint:PI0384|5end_probable_radical_SAM_domain_protein_352884..353114_POSITIVE 293 420 371 226 173 pint:PI0857|5end_hypothetical_protein_790697..790927_POSITIVE 293 419 371 220 158 pint:PI0754|5end_possible_outer_membrane_receptor_683771..684001_POSITIVE 293 428 381 107 64 pint:PI0216|5end_transposase/integrase_209176..209406_POSITIVE 290 423 381 176 132 pint:PI1476|5end_acyl-CoA_synthase/O-succinylbenzoic_acid-CoA_ligase_1397699..1397929_POSITIVE 287 410 364 160 111 pint:PI0558|5end_probable_transcriptional_regulator,_TetR_family_507036..507266_POSITIVE 285 410 361 111 47 pint:PI0551|5end_hypothetical_protein_502328..502558_POSITIVE 284 437 405 189 148 pint:PI0739|5end_conserved_hypothetical_protein_671794..672024_POSITIVE 282 429 381 183 118 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 281 430 381 108 50 pint:PI2140|5end_conserved_hypothetical_protein_2086773..2087003_POSITIVE 281 430 381 78 31 pint:PI0315|5end_conserved_hypothetical_protein_296679..296909_POSITIVE 277 429 381 129 78 pint:PI1572|5end_hypothetical_protein_1497782..1498012_POSITIVE 272 429 384 122 79 pint:PI0729|5end_integrase_661428..661658_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 440 392 81 35 pint:PI1634|3end_conserved_hypothetical_protein_1553819..1553969_POSITIVE 317 436 391 98 45 pint:PI0221|3end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_217796..217946_POSITIVE 309 420 374 140 81 pint:PI2045|3end_hypothetical_protein_1998723..1998873_POSITIVE 295 421 387 46 12 pint:PI1726|3end_ATP-dependent_DNA_helicase_1645542..1645692_POSITIVE 287 428 384 151 108 pint:PI1055|3end_aspartokinase_976302..976452_POSITIVE 277 435 405 117 88 pint:PI0676|3end_hypothetical_protein_614835..614985_POSITIVE 272 429 384 122 79 pint:PI0728|3end_integrase_661508..661658_POSITIVE 271 418 380 36 8 pint:PI0765|3end_conserved_hypothetical_protein;_possible_surface_protein_700822..700972_POSITIVE 271 430 383 113 60 pint:PI0097|3end_aspartate_aminotransferase_94682..94832_POSITIVE 270 437 391 73 28 pint:PI1857|3end_guanylate_kinase_1804396..1804546_POSITIVE 270 417 373 130 70 pint:PI0982|3end_hypothetical_protein_909649..909799_POSITIVE 266 429 382 62 16 pint:PI1647|3end_hypothetical_protein_1562531..1562681_POSITIVE 266 429 382 62 16 pint:PI0353|3end_hypothetical_protein_325337..325487_POSITIVE 265 422 382 52 3 pint:PI1408|3end_glucose-1-phosphate_thymidylyltransferase_1329761..1329911_POSITIVE 265 438 397 111 63 pint:PI0979|3end_transcriptional_regulator,_tetR_family_905678..905828_POSITIVE 264 424 381 62 7 pint:PI1046|3end_hypothetical_protein_966081..966231_POSITIVE 262 429 381 135 77 pint:PI1089|3end_conserved_hypothetical_protein_1014040..1014190_POSITIVE 260 419 371 111 51 pint:PI1226|3end_hypothetical_protein_1148263..1148413_POSITIVE 257 430 382 87 31 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 255 435 391 113 73 pint:PI0769|3end_phosphomannomutase_706172..706322_POSITIVE