Origin IGS: taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatctattaattactgtttcgatttctattaaactggaaccaccattgcggtttccaatatgttgctcttaaatagttgaacaatacaaaaggccaactaaatgatttaagaacgctgcaaagataagtaatttctcctaaagtcttttcccccttaacagtttttattgtatctaaaaatcagcgagttgccttcattatatatttgtatatttacgtttcacccccttgtattaggcgtttcaagtcctttttaaaaaacgtgttta Mask Tandem Repeat Region ================================================ taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Find is-nt database================================================ Query_seq: PI1826:PI1827|PI1826:PI1827:ATP-dependent DNA helicase:peptidase, M23/M37 family, N-terminal:->->:1767884..1768152 269 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1826:PI1827|PI1826:PI1827:ATP-dependent DNA helicase:peptidase, M23/M37 family, N-terminal:->->:1767884..1768152 269 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1826:PI1827|PI1826:PI1827:ATP-dependent DNA helicase:peptidase, M23/M37 family, N-terminal:->->:1767884..1768152 269 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 102 End: 260 ..................................................................................................... M K T V K G E K T L G E I T Y L C S V L K S F S W P F V L F N Y L R A T Y W K P Q W W F Q F N R N R N S N ......... Protein_Len: 46 Strand: - Start: 2 End: 139 . F V N K L F S K F R R I C P P S V Y I Y L Y I I F A V R Q N K S V I F V T L P S F V K P S M .................................................................................................................................. taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: - Score: 81 Start: 220 End: 236 ...........................................................................................................................................................................................................................CTGGAACCACCATTGCG................................. taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1826:PI1827|PI1826:PI1827:ATP-dependent DNA helicase:peptidase, M23/M37 family, N-terminal:->->:1767884..1768152 269 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Intra-Species Hit: Count: 1 Min: 1 Max: 269 Len: 269 Subject: pint_PI1826_PI1827|ATP-dependent DNA helicase:peptidase, M23/M37 family, N-terminal|POSITIVE:POSITIVE|[1767884,1768152]|269 HSP 1 e-value: 1.0E-150 bit: 533.0 Len: 269 Query Start:1 Query End:269 Subject Strand: POSITIVE Subject Start: 1 Subject End: 269 taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata taaacacgttttttaaaaaggacttgaaacgcctaatacaagggggtgaaacgtaaatatacaaatatataatgaaggcaactcgctgatttttagatacaataaaaactgttaagggggaaaagactttaggagaaattacttatctttgcagcgttcttaaatcatttagttggccttttgtattgttcaactatttaagagcaacatattggaaaccgcaatggtggttccagtttaatagaaatcgaaacagtaattaatagata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACACGUUUUUUAAAAAGGACUUGAAACGCCUAAUACAAGGGGGUGAAACGUAAAUAUACAAAUAUAUAAUGAAGGCAACUCGCUGAUUUUUAGAUACAAUAAAAACUGUUAAGGGGGAAAAGACUUUAGGAGAAAUUACUUAUCUUUGCAGCGUUCUUAAAUCAUUUAGUUGGCCUUUUGUAUUGUUCAACUAUUUAAGAGCAACAUAUUGGAAACCGCAAUGGUGGUUCCAGUUUAAUAGAAAUCGAAACAGUAAUUAAUAGAUA ......((.((((((.......((((((.....((((((((((((.(((...(((....)))........(((((.(....).(((((......(((((...(((...((.((((((.........)))))).))...)))..)))))...)))))........)))))...))).))))))))))))........))))))........(((((((.((((....)))))))))))....)))))).))................... (-49.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCUAUUAAUUACUGUUUCGAUUUCUAUUAAACUGGAACCACCAUUGCGGUUUCCAAUAUGUUGCUCUUAAAUAGUUGAACAAUACAAAAGGCCAACUAAAUGAUUUAAGAACGCUGCAAAGAUAAGUAAUUUCUCCUAAAGUCUUUUCCCCCUUAACAGUUUUUAUUGUAUCUAAAAAUCAGCGAGUUGCCUUCAUUAUAUAUUUGUAUAUUUACGUUUCACCCCCUUGUAUUAGGCGUUUCAAGUCCUUUUUAAAAAACGUGUUUA ...................................((((((........)))))).((((((((....(((((.(((((((..(((((((((((.((((...((((((.(((.......((((((...((........))..)))))).........(((((....))))).)))..))))))...))))))))..........)))))))...............(((......)))...)))))...)).)))))..)))))))).. (-36.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 50 3 226 173 pint:PI1028|5end_glutamate_synthase,_small_subunit_943420..943650_POSITIVE 296 69 22 103 61 pint:PI2029|5end_DNA_topoisomerase_I_1982775..1983005_POSITIVE 285 247 203 213 175 pint:PI0889|5end_pyruvate_kinase_818758..818988_POSITIVE 275 244 204 39 3 pint:PI0571|5end_conserved_hypothetical_protein;_possible_permease_515161..515391_POSITIVE 273 229 182 68 10 pint:PI0005|5end_hypothetical_protein_6984..7214_POSITIVE 268 237 191 100 49 pint:PI1918|5end_uracil-DNA_glycosylase_1861566..1861796_POSITIVE 264 250 201 71 13 pint:PI1071|5end_DNA_topoisomerase_III_988290..988520_POSITIVE 261 230 181 60 6 pint:PI2177|5end_phosphoribosylaminoimidazole_carboxylase_2117127..2117357_POSITIVE 256 88 41 159 98 pint:PI1189|5end_conserved_hypothetical_protein_1107114..1107344_POSITIVE 255 111 71 123 71 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 252 177 143 84 51 pint:PI0419|5end_conserved_hypothetical_protein_386701..386931_POSITIVE 252 177 143 49 16 pint:PI0418|5end_hypothetical_protein_386666..386896_POSITIVE 251 51 19 167 140 pint:PI1536|5end_hypothetical_protein_1455555..1455785_POSITIVE 250 88 43 48 5 pint:PI1247|5end_conserved_hypothetical_protein_1171630..1171860_POSITIVE 249 219 172 145 93 pint:PI1461|5end_conserved_hypothetical_protein_1384364..1384594_POSITIVE 249 180 131 223 176 pint:PI0424|5end_methylated-DNA--[protein]-cysteine_S-methyltransferase_388429..388659_POSITIVE 248 230 189 157 116 pint:PI1981|5end_hypothetical_protein_1937897..1938127_POSITIVE 247 209 170 144 91 pint:PI0727|5end_integrase_659261..659491_POSITIVE 247 49 4 144 96 pint:PI0087|5end_conserved_hypothetical_protein_85700..85930_POSITIVE 245 159 116 86 52 pint:PI1290|5end_ATP_synthase,_beta_subunit_1203059..1203289_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 50 3 125 72 pint:PI1027|3end_hypothetical_protein_943399..943549_POSITIVE 296 69 22 50 8 pint:PI2028|3end_shikimate_kinase_1982802..1982952_POSITIVE 283 55 19 58 21 pint:PI0194|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_(glycine_C-acetyltransferase)_186957..187107_POSITIVE 270 252 211 50 5 pint:PI1882|3end_hypothetical_protein_1826487..1826637_POSITIVE 255 158 111 143 87 pint:PI0102|3end_hypothetical_protein_98064..98214_POSITIVE 252 177 143 131 98 pint:PI0420|3end_hypothetical_protein_386828..386978_POSITIVE 251 51 19 33 6 pint:PI1535|3end_response_regulator_1455501..1455651_POSITIVE 249 199 153 137 90 pint:PI1708|3end_hypothetical_protein_1625562..1625712_POSITIVE 249 219 172 137 85 pint:PI1460|3end_ribonucleotide_reductase,_alpha_subunit,_N-terminal_region_1384436..1384586_POSITIVE 249 180 131 146 99 pint:PI0423|3end_hypothetical_protein_388432..388582_POSITIVE 248 230 189 125 84 pint:PI1980|3end_conserved_hypothetical_protein_1937945..1938095_POSITIVE 247 218 171 75 21 pint:PI1177|3end_hypothtical_protein_1099154..1099304_POSITIVE 247 209 170 56 3 pint:PI0726|3end_hypothetical_protein_659253..659403_POSITIVE 247 49 4 134 86 pint:PI0086|3end_possible_uroporphyrinogen-III_synthase_85770..85920_POSITIVE 245 159 116 55 21 pint:PI1289|3end_ATP_synthase,_delta/epsilon_subunit_1203108..1203258_POSITIVE 243 180 131 141 79 pint:PI0522|3end_50S_ribosomal_protein_L19_479449..479599_POSITIVE 243 177 144 33 1 pint:PI0417|3end_hypothetical_protein_386730..386880_POSITIVE 242 237 195 50 6 pint:PI1917|3end_uracil-DNA_glycosylase_1861596..1861746_POSITIVE 240 240 191 90 40 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 237 118 73 83 25 pint:PI0426|3end_conserved_hypothetical_protein_391206..391356_POSITIVE