Origin IGS: aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatatggcaaaggcatacgaatttcttgctaatggctttgaagatattgaggctttagcacccgtagacattctccgcagaggaggcgttgaagtgaaaacagtaagtatttccgacaattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaattgtcggaaatacttactgttttcacttcaacgcctcctctgcggagaatgtctacgggtgctaaagcctcaatatcttcaaagccattagcaagaaattcgtatgcctttgccatatatttttcgtttaaatctttaacattccaacttatttcgttatttttgcaaaagaaaacgattgattaagactttttggcacaatcgccataaagatgcttaacaatacgtttcgctgcttaaaaacccaaatgcaaatatacaatataattcacaatttggcgaaaaagtagcgtagaaaagatttattatgggcagcatttgaaggctggaacatatttaaaacagaatattt Mask Tandem Repeat Region ================================================ aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatatggcaaaggcatacgaatttcttgctaatggctttgaagatattgaggctttagcacccgtagacattctccgcagaggaggcgttgaagtgaaaacagtaagtatttccgacaattta Find is-nt database================================================ Query_seq: PI1823:PI1824|PI1823:PI1824:inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase):ThiJ/PfpI family protein:->->:1764263..1764618 356 aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatatggcaaaggcatacgaatttcttgctaatggctttgaagatattgaggctttagcacccgtagacattctccgcagaggaggcgttgaagtgaaaacagtaagtatttccgacaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1823:PI1824|PI1823:PI1824:inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase):ThiJ/PfpI family protein:->->:1764263..1764618 356 aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatatggcaaaggcatacgaatttcttgctaatggctttgaagatattgaggctttagcacccgtagacattctccgcagaggaggcgttgaagtgaaaacagtaagtatttccgacaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1823:PI1824|PI1823:PI1824:inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase):ThiJ/PfpI family protein:->->:1764263..1764618 356 aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatatggcaaaggcatacgaatttcttgctaatggctttgaagatattgaggctttagcacccgtagacattctccgcagaggaggcgttgaagtgaaaacagtaagtatttccgacaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 237 Max: 353 Len: 117 Subject: UniRef90_Q1FJV6 Cluster: DJ-1; n=1; Clostridium phytofermentans ISDg|Rep: DJ-1 - Clostridium phytofermentans ISDg HSP 1 e-value: 3.0E-7 bit: 56.6 Len: 117 Query Start:237 Query End:353 Subject Strand: null Subject Start: 1 Subject End: 39 ............................................................................................................................................................................................................................................ M A K A Y E F L A N G F E D I E A L A P V D I L R R G G V E V K T V S I S D N ... ............................................................................................................................................................................................................................................ M A K V Y V F L A D G F E E I E A L T V V D L L R R A G V D V T T V S I T E N ... Subject: UniRef90_Q73JT5 Cluster: DJ-1 family protein; n=1; Treponema denticola|Rep: DJ-1 family protein - Treponema denticola HSP 1 e-value: 8.0E-6 bit: 51.6 Len: 108 Query Start:237 Query End:344 Subject Strand: null Subject Start: 1 Subject End: 36 ............................................................................................................................................................................................................................................ M A K A Y E F L A N G F E D I E A L A P V D I L R R G G V E V K T V S I ............ ............................................................................................................................................................................................................................................ M K K A F L F L A N G F E D V E A L T P I D Y L R R A G I D L I T V G V ............ aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 17 End: 133 ................ M C S S L Q M L P I I N L F Y A T F S P N C E L Y C I F A F G F L S S E T Y C ............................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 85 End: 201 .................................................................................... M I L Y I C I W V F K Q R N V L L S I F M A I V P K S L N Q S F S F A K I T K ........................................................................................................................................................... Protein_Len: 60 Strand: + Start: 174 End: 356 ............................................................................................................................................................................. M V F F C K N N E I S W N V K D L N E K Y M A K A Y E F L A N G F E D I E A L A P V D I L R R G G V E V K T V S I S D N Protein_Len: 60 Strand: - Start: 170 End: 352 ......................................................................................................................................................................... I T K K Q L F L S I L Q F T L S K F S F Y I A F A Y S N R A L P K S S I S A K A G T S M R R L P P T S T F V T L I E S M .... aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 70 End: 99 .....................................................................TTCGCCAAATTGTGAATTATATTGTATATT................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1823:PI1824|PI1823:PI1824:inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase):ThiJ/PfpI family protein:->->:1764263..1764618 356 aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntta Intra-Species Hit: Count: 1 Min: 1 Max: 236 Len: 236 Subject: pint_PI1823_PI1824|inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase):ThiJ/PfpI family protein|POSITIVE:POSITIVE|[1764263,1764618]|356 HSP 1 e-value: 1.0E-131 bit: 468.0 Len: 236 Query Start:1 Query End:236 Subject Strand: POSITIVE Subject Start: 1 Subject End: 236 aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatat........................................................................................................................ aaatattctgttttaaatatgttccagccttcaaatgctgcccataataaatcttttctacgctactttttcgccaaattgtgaattatattgtatatttgcatttgggtttttaagcagcgaaacgtattgttaagcatctttatggcgattgtgccaaaaagtcttaatcaatcgttttcttttgcaaaaataacgaaataagttggaatgttaaagatttaaacgaaaaatat........................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAUUCUGUUUUAAAUAUGUUCCAGCCUUCAAAUGCUGCCCAUAAUAAAUCUUUUCUACGCUACUUUUUCGCCAAAUUGUGAAUUAUAUUGUAUAUUUGCAUUUGGGUUUUUAAGCAGCGAAACGUAUUGUUAAGCAUCUUUAUGGCGAUUGUGCCAAAAAGUCUUAAUCAAUCGUUUUCUUUUGCAAAAAUAACGAAAUAAGUUGGAAUGUUAAAGAUUUAAACGAAAAAUAU ........((((((...((((...((((........))))..)))).(((((((((..((..(((((((((((((((((.(..(((..........)))..)))))))........((((.((((((.(((((((((...((((.(((((....))))).)))).))))).))))))))))...))))........))))).))).)))..))..))))))))).....)))))). (-51.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUUUUCGUUUAAAUCUUUAACAUUCCAACUUAUUUCGUUAUUUUUGCAAAAGAAAACGAUUGAUUAAGACUUUUUGGCACAAUCGCCAUAAAGAUGCUUAACAAUACGUUUCGCUGCUUAAAAACCCAAAUGCAAAUAUACAAUAUAAUUCACAAUUUGGCGAAAAAGUAGCGUAGAAAAGAUUUAUUAUGGGCAGCAUUUGAAGGCUGGAACAUAUUUAAAACAGAAUAUUU (((((((...(((((((..........(((...((..((....(((((((......(((((((((.(((((.((((.((((......)))).))))...))))))))).))))).(((((((......((((((.......................)))))).....)))))))))))))).))..))...))).((((........))))......)))))))..))))))).. (-46.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 157 119 156 111 pint:PI0733|5end_membrane_protein;_predicted_exporter_664389..664619_POSITIVE 291 43 6 71 32 pint:PI2057|5end_conserved_hypothetical_protein;_possible_metal-dependent_membrane_protease_2014939..2015169_POSITIVE 283 172 136 35 2 pint:PI1068|5end_nucleolar_protein,_Sun_(Fmu)_family_protein;_tRNA/rRNA_cytosine-C5-methylase_984743..984973_POSITIVE 278 60 18 108 55 pint:PI0465|5end_50S_ribosomal_protein_L22_435306..435536_POSITIVE 273 142 101 60 19 pint:PI0839|5end_conserved_hypothetical_protein_773843..774073_POSITIVE 267 150 101 120 72 pint:PI1671|5end_hypothetical_protein_1586397..1586627_POSITIVE 266 139 91 54 1 pint:PI1387|5end_cardiolipin_synthetase_1308021..1308251_POSITIVE 264 150 102 202 150 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 262 60 23 164 131 pint:PI0604|5end_O-succinylbenzoic_acid--CoA_ligase_550825..551055_POSITIVE 261 49 24 213 182 pint:PI0125|5end_undecaprenyl_pyrophosphate_synthase_117106..117336_POSITIVE 259 189 141 192 138 pint:PI2135|5end_conserved_hypothetical_protein_2082754..2082984_POSITIVE 257 160 115 60 23 pint:PI1741|5end_lipid-A-disaccharide_synthase_1662754..1662984_POSITIVE 256 159 111 195 137 pint:PI1999|5end_hypothetical_protein_1956457..1956687_POSITIVE 255 120 73 113 68 pint:PI1467|5end_conserved_hypothetical_protein_1393503..1393733_POSITIVE 254 149 107 56 3 pint:PI1617|5end_hypothetical_protein_1542923..1543153_POSITIVE 253 126 83 168 115 pint:PI0926|5end_hypothetical_protein_856824..857054_POSITIVE 249 109 61 213 164 pint:PI2170|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_2112238..2112468_POSITIVE 249 166 126 57 20 pint:PI1795|5end_conserved_hypothetical_protein_1726864..1727094_POSITIVE 249 159 116 49 17 pint:PI1477|5end_TonB-dependent_outer_membrane_receptor_1399734..1399964_POSITIVE 249 160 116 75 31 pint:PI0773|5end_D-3-phosphoglycerate_dehydrogenase_708498..708728_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 157 119 54 9 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 278 60 18 73 20 pint:PI0464|3end_30S_ribosomal_protein_S19_435351..435501_POSITIVE 273 142 101 64 23 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 262 60 23 100 67 pint:PI0603|3end_3-dehydroquinate_synthase_550841..550991_POSITIVE 257 156 116 66 29 pint:PI0324|3end_conserved_hypothetical_protein_305680..305830_POSITIVE 253 156 116 150 111 pint:PI1300|3end_probable_glycosyltransferase_1214370..1214520_POSITIVE 250 47 6 151 106 pint:PI0424|3end_methylated-DNA--[protein]-cysteine_S-methyltransferase_388976..389126_POSITIVE 243 75 33 137 93 pint:PI1826|3end_ATP-dependent_DNA_helicase_1767823..1767973_POSITIVE 243 75 37 114 61 pint:PI1313|3end_possible_POT_family_(proton-dependent_oligopeptide_transport_family)_1231241..1231391_POSITIVE 238 148 105 54 9 pint:PI1671|3end_hypothetical_protein_1586653..1586803_POSITIVE 237 128 96 107 67 pint:PI1631|3end_hypothetical_protein_1552797..1552947_POSITIVE 236 159 115 82 32 pint:PI0991|3end_conserved_hypothetical_protein_916459..916609_POSITIVE 236 177 136 74 36 pint:PI0984|3end_hypothetical_protein_910144..910294_POSITIVE 236 149 101 125 79 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 236 189 145 53 14 pint:PI0367|3end_hypothetical_protein_339640..339790_POSITIVE 234 159 121 45 5 pint:PI0821|3end_conserved_hypothetical_protein_761213..761363_POSITIVE 232 178 137 63 20 pint:PI0417|3end_hypothetical_protein_386730..386880_POSITIVE 231 189 147 63 31 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 231 158 116 43 5 pint:PI0024|3end_hypothetical_protein_28559..28709_POSITIVE 230 157 113 60 15 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE