Origin IGS: taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaattttccgagaagcggatcaggcagcatatcgcagttcagcttgttttgctgccttgaattaaaagaaatgattgcttccatcgttgtttaaataagctattaaacgacacgaaaggtacggattgaacatataggggcgtgatttaccactttcctgacaccgaagaaaaagtaggttggatataaggagcgtgatgaattgcgccctta Mask Tandem Repeat Region ================================================ taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Find is-nt database================================================ Query_seq: PI1820:PI1821|PI1820:PI1821:hypothetical protein:possible thioredoxin family protein:->->:1760438..1760653 216 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1820:PI1821|PI1820:PI1821:hypothetical protein:possible thioredoxin family protein:->->:1760438..1760653 216 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1820:PI1821|PI1820:PI1821:hypothetical protein:possible thioredoxin family protein:->->:1760438..1760653 216 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 15 End: 149 .............. M T L L I S N L L F L R C Q E S G K S R P Y M F N P Y L S C R L I A Y L N N D G S N H F F ................................................................... Protein_Len: 35 Strand: + Start: 68 End: 172 ................................................................... M T P L Y V Q S V P F V S F N S L F K Q R W K Q S F L L I Q G S K T S ............................................ taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1820:PI1821|PI1820:PI1821:hypothetical protein:possible thioredoxin family protein:->->:1760438..1760653 216 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Intra-Species Hit: Count: 1 Min: 1 Max: 216 Len: 216 Subject: pint_PI1820_PI1821|hypothetical protein:possible thioredoxin family protein|POSITIVE:POSITIVE|[1760438,1760653]|216 HSP 1 e-value: 1.0E-119 bit: 428.0 Len: 216 Query Start:1 Query End:216 Subject Strand: POSITIVE Subject Start: 1 Subject End: 216 taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta taagggcgcaattcatcacgctccttatatccaacctactttttcttcggtgtcaggaaagtggtaaatcacgcccctatatgttcaatccgtacctttcgtgtcgtttaatagcttatttaaacaacgatggaagcaatcatttcttttaattcaaggcagcaaaacaagctgaactgcgatatgctgcctgatccgcttctcggaaaattctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGGCGCAAUUCAUCACGCUCCUUAUAUCCAACCUACUUUUUCUUCGGUGUCAGGAAAGUGGUAAAUCACGCCCCUAUAUGUUCAAUCCGUACCUUUCGUGUCGUUUAAUAGCUUAUUUAAACAACGAUGGAAGCAAUCAUUUCUUUUAAUUCAAGGCAGCAAAACAAGCUGAACUGCGAUAUGCUGCCUGAUCCGCUUCUCGGAAAAUUCUUA ...(((((..(((.(((((..((((.(((((.................))))).))))..))))).)))..)))))............((((......((((...((((((........)))))).)))).((((((....(((......)))(((.(((((((......((......))....))))))))))...))))))))))......... (-56.43) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAUUUUCCGAGAAGCGGAUCAGGCAGCAUAUCGCAGUUCAGCUUGUUUUGCUGCCUUGAAUUAAAAGAAAUGAUUGCUUCCAUCGUUGUUUAAAUAAGCUAUUAAACGACACGAAAGGUACGGAUUGAACAUAUAGGGGCGUGAUUUACCACUUUCCUGACACCGAAGAAAAAGUAGGUUGGAUAUAAGGAGCGUGAUGAAUUGCGCCCUUA .........((((.(((((((.((((((((((....((((......))))..))))))).)))......(....)..)))))))....(((((((((........))))))))).(....)...)))).........((((((((..(((((.(((.(((((....((((............)))).....))))).))).)))))..)))))))) (-67.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 495 90 45 180 131 pint:PI0902|5end_hypothetical_protein_832291..832521_POSITIVE 413 76 36 177 138 pint:PI0866|5end_hypothetical_protein_803776..804006_POSITIVE 413 90 41 155 102 pint:PI0069|5end_hypothetical_protein_67072..67302_POSITIVE 379 80 36 124 86 pint:PI0118|5end_hypothetical_protein_111321..111551_POSITIVE 364 199 151 58 9 pint:PI0572|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_516382..516612_POSITIVE 345 45 2 165 121 pint:PI0799|5end_alpha-amylase_family_protein_737083..737313_POSITIVE 341 202 163 228 191 pint:PI0028|5end_aminopeptidase_31559..31789_POSITIVE 336 77 31 171 104 pint:PI0762|5end_mannose-6-phosphate_isomerase,_class_I_696420..696650_POSITIVE 335 199 153 77 37 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 327 76 31 185 140 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 326 195 158 45 8 pint:PI0060|5end_long-chain-fatty-acid--CoA_ligase_(acyl-CoA_synthetase)_58281..58511_POSITIVE 324 49 2 123 82 pint:PI0943|5end_conserved_hypothetical_protein_875153..875383_POSITIVE 323 110 69 230 177 pint:PI1497|5end_LuxS_protein,_autoinducer_AI-2_production_protein_1417571..1417801_POSITIVE 321 200 151 74 17 pint:PI0180|5end_TonB_protein_171905..172135_POSITIVE 318 90 48 50 4 pint:PI1050|5end_conserved_hypothetical_protein_967246..967476_POSITIVE 317 97 51 68 22 pint:PI0035|5end_NAD-dependent_protein_deacetylase,_SIR2_family_37873..38103_POSITIVE 311 78 49 136 107 pint:PI1749|5end_conserved_hypothetical_protein_1671039..1671269_POSITIVE 309 199 159 193 154 pint:PI2115|5end_hypothetical_protein_2064966..2065196_POSITIVE 305 207 170 132 93 pint:PI1363|5end_hypothetical_protein_1281666..1281896_POSITIVE 305 88 50 227 177 pint:PI1312|5end_conserved_hypothetical_protein_1228851..1229081_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 541 82 41 53 12 pint:PI2088|3end_conserved_hypothetical_protein;_possible_PAP2_superfamily_protein_2045815..2045965_POSITIVE 421 86 43 46 1 pint:PI0962|3end_phosphoglycerate_mutase_892702..892852_POSITIVE 411 79 38 72 31 pint:PI1440|3end_conserved_hypothetical_protein_1363009..1363159_POSITIVE 356 80 45 37 2 pint:PI0117|3end_ribonuclease_HI_111314..111464_POSITIVE 343 199 157 45 3 pint:PI0571|3end_conserved_hypothetical_protein;_possible_permease_516449..516599_POSITIVE 335 199 153 68 28 pint:PI2016|3end_ribosomal_large_subunit_pseudouridine_synthase_D_1974933..1975083_POSITIVE 324 49 2 139 98 pint:PI0944|3end_hypothetical_protein_875249..875399_POSITIVE 318 90 48 65 19 pint:PI1049|3end_hypothetical_protein_967341..967491_POSITIVE 300 50 1 128 77 pint:PI0664|3end_dihydroorotate_dehydrogenase,_electron_transfer_subunit_607819..607969_POSITIVE 293 200 158 63 16 pint:PI1467|3end_conserved_hypothetical_protein_1393909..1394059_POSITIVE 292 197 151 63 13 pint:PI2028|3end_shikimate_kinase_1982802..1982952_POSITIVE 291 206 161 143 97 pint:PI0260|3end_conjugative_transposon_protein,_TraJ_254122..254272_POSITIVE 290 206 171 76 40 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 290 209 171 64 21 pint:PI0179|3end_hypothetical_protein_171959..172109_POSITIVE 287 199 158 38 7 pint:PI0264|3end_transfer_region-related_protein,_TraN_257371..257521_POSITIVE 281 206 168 98 54 pint:PI0728|3end_integrase_661508..661658_POSITIVE 278 198 155 147 107 pint:PI0790|3end_hypothetical_protein_729206..729356_POSITIVE 278 198 155 50 10 pint:PI0791|3end_conserved_hypothetical_protein_729109..729259_POSITIVE 278 76 31 56 16 pint:PI0504|3end_hypothetical_protein_464658..464808_POSITIVE 277 189 141 131 74 pint:PI1923|3end_conserved_hypothetical_protein_1865912..1866062_POSITIVE