Origin IGS: gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtcataaggagttacagaaggctttacttccgtaactcctatatcaaggcatttcagcttaataccaaaacaaaaagtaaactaatataaaagatagaccaatgaaaagaattatacttttcgccacttgcagcttaatggtgtgcgcaggtgccttcgctcaacagaaagaagagccgaagttagttgttaagccatcaggacgtattctc Mask Tandem Repeat Region ================================================ gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Find is-nt database================================================ Query_seq: PI1815:PI1816|PI1815:PI1816:polyphosphate-selective porin O:acid phosphatase:->->:1754571..1754785 215 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1815:PI1816|PI1815:PI1816:polyphosphate-selective porin O:acid phosphatase:->->:1754571..1754785 215 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1815:PI1816|PI1815:PI1816:polyphosphate-selective porin O:acid phosphatase:->->:1754571..1754785 215 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: - Start: 1 End: 126 L I R G S P K V V L K P E E K Q Q A F A G A C V M L S C T A F L I I R K M P R D K M ......................................................................................... gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1815:PI1816|PI1815:PI1816:polyphosphate-selective porin O:acid phosphatase:->->:1754571..1754785 215 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Intra-Species Hit: Count: 1 Min: 1 Max: 215 Len: 215 Subject: pint_PI1815_PI1816|polyphosphate-selective porin O:acid phosphatase|POSITIVE:POSITIVE|[1754571,1754785]|215 HSP 1 e-value: 1.0E-118 bit: 426.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta gagaatacgtcctgatggcttaacaactaacttcggctcttctttctgttgagcgaaggcacctgcgcacaccattaagctgcaagtggcgaaaagtataattcttttcattggtctatcttttatattagtttactttttgttttggtattaagctgaaatgccttgatataggagttacggaagtaaagccttctgtaactccttatgactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAAUACGUCCUGAUGGCUUAACAACUAACUUCGGCUCUUCUUUCUGUUGAGCGAAGGCACCUGCGCACACCAUUAAGCUGCAAGUGGCGAAAAGUAUAAUUCUUUUCAUUGGUCUAUCUUUUAUAUUAGUUUACUUUUUGUUUUGGUAUUAAGCUGAAAUGCCUUGAUAUAGGAGUUACGGAAGUAAAGCCUUCUGUAACUCCUUAUGACUUA ........(((((..((((((((.......(((((.(((..(.....)..))))))))((....))........))))))))..)).)))((((((.......))))))...((((........................((((..((((((.......))))))..)))).((((((((((((((......))))))))))))))...)))).. (-55.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUCAUAAGGAGUUACAGAAGGCUUUACUUCCGUAACUCCUAUAUCAAGGCAUUUCAGCUUAAUACCAAAACAAAAAGUAAACUAAUAUAAAAGAUAGACCAAUGAAAAGAAUUAUACUUUUCGCCACUUGCAGCUUAAUGGUGUGCGCAGGUGCCUUCGCUCAACAGAAAGAAGAGCCGAAGUUAGUUGUUAAGCCAUCAGGACGUAUUCUC ...(((...(((((((((.((((......)))).))))))))).......(((.(..(((((............................................((((((.......))))))..(((((((.((..........))))))))).((((((((..(.....)..))).)))))..)))))..).))).....)))........ (-54.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 415 90 45 216 167 pint:PI0260|5end_conjugative_transposon_protein,_TraJ_253023..253253_POSITIVE 404 98 51 118 65 pint:PI0705|5end_conserved_hypothetical_protein;_possible_lipoprotein_641274..641504_POSITIVE 361 90 43 193 149 pint:PI0264|5end_transfer_region-related_protein,_TraN_256494..256724_POSITIVE 347 66 30 127 93 pint:PI1402|5end_3-deoxy-7-phosphoheptulonate_synthase_1322935..1323165_POSITIVE 346 68 32 196 157 pint:PI1360|5end_conserved_hypothetical_protein_1275923..1276153_POSITIVE 334 90 48 54 1 pint:PI1697|5end_conserved_hypothetical_protein_1612217..1612447_POSITIVE 332 90 42 169 118 pint:PI1747|5end_membrane-bound_lytic_murein_transglycosylase_D_1667483..1667713_POSITIVE 332 80 33 96 45 pint:PI1705|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_III_1621781..1622011_POSITIVE 332 90 50 159 110 pint:PI0094|5end_protoporphyrinogen_oxidase_89571..89801_POSITIVE 332 90 50 85 36 pint:PI0091|5end_hypothetical_protein_89497..89727_POSITIVE 329 90 45 227 181 pint:PI0370|5end_conserved_hypothetical_protein_340453..340683_POSITIVE 327 92 52 48 2 pint:PI0488|5end_conserved_hypothetical_protein_446906..447136_POSITIVE 327 69 30 218 180 pint:PI0147|5end_HIT_family_protein_140833..141063_POSITIVE 324 78 31 73 13 pint:PI0602|5end_conserved_hypothetical_protein_549099..549329_POSITIVE 324 98 63 114 84 pint:PI0564|5end_hypothetical_protein_511537..511767_POSITIVE 324 98 63 56 26 pint:PI0565|5end_hypothetical_protein_511479..511709_POSITIVE 324 86 50 53 15 pint:PI0285|5end_succinyltransferase_protein_270097..270327_POSITIVE 323 60 12 80 30 pint:PI1637|5end_hypothetical_protein_1554500..1554730_POSITIVE 321 76 38 172 126 pint:PI0927|5end_transglutaminase-related_protein_857283..857513_POSITIVE 321 98 51 182 129 pint:PI0781|5end_UDP-glucose/GDP-mannose_dehydrogenase_family;_probable__UDP-glucose_6-dehydrogenase_719260..719490_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 404 98 51 128 75 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 367 83 45 148 107 pint:PI0259|3end_conserved_protein_found_in_conjugation,_TraI_253043..253193_POSITIVE 357 73 31 138 102 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE 357 97 51 105 55 pint:PI0592|3end_DNA_mismatch_repair_protein_542611..542761_POSITIVE 352 90 42 81 27 pint:PI0286|3end_ISPg3-related_transposase_272065..272215_POSITIVE 339 98 52 149 94 pint:PI1882|3end_hypothetical_protein_1826487..1826637_POSITIVE 334 90 48 95 53 pint:PI0183|3end_hypothetical_protein_174810..174960_POSITIVE 329 90 45 103 57 pint:PI0369|3end_hypothetical_protein_340409..340559_POSITIVE 318 89 44 148 105 pint:PI0565|3end_hypothetical_protein_511657..511807_POSITIVE 311 68 37 133 101 pint:PI2062|3end_type_I_restriction-modification_system_2024008..2024158_POSITIVE 311 94 53 58 20 pint:PI0950|3end_50S_ribosomal_protein_L31_883655..883805_POSITIVE 309 83 41 52 6 pint:PI1010|3end_conserved_hypothetical_protein_932195..932345_POSITIVE 304 88 49 117 77 pint:PI1490|3end_GTP_cyclohydrolase_I_1409127..1409277_POSITIVE 303 89 47 62 23 pint:PI1082|3end_polypeptide_deformylase_1001922..1002072_POSITIVE 303 69 26 137 90 pint:PI0756|3end_50S_ribosomal_protein_L7/L12_688980..689130_POSITIVE 303 93 52 150 105 pint:PI0408|3end_hypothetical_protein_374728..374878_POSITIVE 299 59 11 144 95 pint:PI2067|3end_hypothetical_protein_2028337..2028487_POSITIVE 299 90 45 68 11 pint:PI0249|3end_conserved_hypothetical_protein_247026..247176_POSITIVE 296 80 33 86 49 pint:PI0779|3end_predicted_TPR-repeat-containing_protein_715800..715950_POSITIVE 293 98 57 60 12 pint:PI0632|3end_NusB_family_protein_(N_utilization_substance_protein_B)_579731..579881_POSITIVE