Origin IGS: atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaactacttaagaaatcagagcaacaaaaccaatacattgtgtgaactcgatataagaaagtagtttaaaaagttataggagtgagatgtttgtttgtaactattcaatttatcaatataaagttacgaaaaaacatctcactcttatgatgattgtaatctaatagccttagctaaaaacacccattgttttgtaaagataattccaacgaaaaacggtaatttcgat Mask Tandem Repeat Region ================================================ atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Find is-nt database================================================ Query_seq: PI1814:PI1815|PI1814:PI1815:hypothetical protein:polyphosphate-selective porin O:->->:1753294..1753523 230 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1814:PI1815|PI1814:PI1815:hypothetical protein:polyphosphate-selective porin O:->->:1753294..1753523 230 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1814:PI1815|PI1814:PI1815:hypothetical protein:polyphosphate-selective porin O:->->:1753294..1753523 230 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 113 End: 211 ................................................................................................................ M D K L N S Y K Q T S H S Y N F L N Y F L I S S S H N V L V L L L ................... atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1814:PI1815|PI1814:PI1815:hypothetical protein:polyphosphate-selective porin O:->->:1753294..1753523 230 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Intra-Species Hit: Count: 1 Min: 1 Max: 230 Len: 230 Subject: pint_PI1814_PI1815|hypothetical protein:polyphosphate-selective porin O|POSITIVE:POSITIVE|[1753294,1753523]|230 HSP 1 e-value: 1.0E-127 bit: 456.0 Len: 230 Query Start:1 Query End:230 Subject Strand: POSITIVE Subject Start: 1 Subject End: 230 atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta atcgaaattaccgtttttcgttggaattatctttacaaaacaatgggtgtttttagctaaggctattagattacaatcatcataagagtgagatgttttttcgtaactttatattgataaattgaatagttacaaacaaacatctcactcctataactttttaaactactttcttatatcgagttcacacaatgtattggttttgttgctctgatttcttaagtagttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCGAAAUUACCGUUUUUCGUUGGAAUUAUCUUUACAAAACAAUGGGUGUUUUUAGCUAAGGCUAUUAGAUUACAAUCAUCAUAAGAGUGAGAUGUUUUUUCGUAACUUUAUAUUGAUAAAUUGAAUAGUUACAAACAAACAUCUCACUCCUAUAACUUUUUAAACUACUUUCUUAUAUCGAGUUCACACAAUGUAUUGGUUUUGUUGCUCUGAUUUCUUAAGUAGUUUA ...(((((..((((((((.((.(((....)))..)).))).)))))..)))))((((....))))...(((....)))...(((.(((((((((((((.((.((((((....(((....))).....)))))).)).))))))))))))).))).......((((((((((......(((((((..(((.(((......))).))).)))).))).....)))))))))) (-52.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACUACUUAAGAAAUCAGAGCAACAAAACCAAUACAUUGUGUGAACUCGAUAUAAGAAAGUAGUUUAAAAAGUUAUAGGAGUGAGAUGUUUGUUUGUAACUAUUCAAUUUAUCAAUAUAAAGUUACGAAAAAACAUCUCACUCUUAUGAUGAUUGUAAUCUAAUAGCCUUAGCUAAAAACACCCAUUGUUUUGUAAAGAUAAUUCCAACGAAAAACGGUAAUUUCGAU ((((((((((.....(((.(((..(((.((........)).)))...))))))......))))))))))....((((((((((((((((((((.(((((((((...................))))))))).)))))))))))))))))))).......((((..((((....))))((((((.....))))))....))))........(((((........))))).. (-57.21) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 188 142 194 148 pint:PI1261|5end_ISPg3-related_transposase,_N-terminal_region_1183347..1183577_POSITIVE 499 188 143 194 150 pint:PI2110|5end_ISPg3-related_transposase_(IS195_family)_2061470..2061700_POSITIVE 499 188 143 194 150 pint:PI1762|5end_ISPg3-related_transposase_1684669..1684899_POSITIVE 499 188 143 194 150 pint:PI1714|5end_ISPg3-related_transposase_1630376..1630606_POSITIVE 499 188 143 194 150 pint:PI0808|5end_ISPg3-related_transposase_745481..745711_POSITIVE 499 188 143 194 150 pint:PI0326|5end_ISPg3-related_transposase_306792..307022_POSITIVE 499 188 143 194 150 pint:PI0172|5end_ISPg3-related_transposase_164262..164492_POSITIVE 475 188 143 194 150 pint:PI0298|5end_ISPg3-related_transposase_283688..283918_POSITIVE 416 46 1 226 184 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 416 43 1 75 32 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 415 50 1 119 71 pint:PI1786|5end_hypothetical_protein_1718124..1718354_POSITIVE 406 40 1 91 52 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 401 49 3 179 134 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 385 47 1 137 92 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 378 49 1 117 70 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 351 57 16 41 1 pint:PI1521|5end_hypothetical_protein_1441220..1441450_POSITIVE 349 59 12 98 51 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 321 57 12 90 45 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 310 158 111 123 77 pint:PI1019|5end_hypothetical_protein_938228..938458_POSITIVE 309 57 23 144 110 pint:PI1204|5end_hypothetical_protein_1124430..1124660_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 499 188 143 102 58 pint:PI1760|3end_hypothetical_protein_1684657..1684807_POSITIVE 499 188 143 66 22 pint:PI0325|3end_ISPg3-related_transposase_fragment_306744..306894_POSITIVE 492 50 1 105 56 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 442 50 1 93 42 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 408 188 150 39 1 pint:PI1260|3end_IS1001-related_transposase_1183272..1183422_POSITIVE 348 70 23 56 10 pint:PI1063|3end_excinuclease_ABC,_subunit_B_983099..983249_POSITIVE 310 158 111 96 50 pint:PI1591|3end_ISPg3-related_transposase_1519723..1519873_POSITIVE 310 158 111 96 50 pint:PI1018|3end_ISPg3-related_transposase,_N-terminal_region_938281..938431_POSITIVE 309 57 23 73 39 pint:PI1203|3end_conserved_hypothetical_protein_1124439..1124589_POSITIVE 299 158 111 96 50 pint:PI0288|3end_hypothetical_protein_(possible_ISPg3-related_transposase)_272495..272645_POSITIVE 291 158 111 96 50 pint:PI2070|3end_ISPg3-related_transposase,_N-terminal_fragment_2029961..2030111_POSITIVE 287 220 171 59 4 pint:PI0620|3end_hypothetical_protein_570630..570780_POSITIVE 270 208 171 44 3 pint:PI0310|3end_outer_membrane_protein_294310..294460_POSITIVE 264 214 174 118 78 pint:PI1662|3end_hypothetical_protein_1577241..1577391_POSITIVE 264 59 24 41 6 pint:PI1577|3end_conserved_hypothetical_protein_1503879..1504029_POSITIVE 247 172 131 151 111 pint:PI2109|3end_surface_antigen_BspA_2061523..2061673_POSITIVE 244 210 171 112 69 pint:PI1934|3end_hypothetical_protein_1882601..1882751_POSITIVE 241 207 161 92 47 pint:PI2178|3end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase,_GcpE_2119730..2119880_POSITIVE 238 158 111 96 50 pint:PI1817|3end_ISPg3-related_transposase_1756496..1756646_POSITIVE 238 158 111 96 50 pint:PI1303|3end_ISPg3-related_transposase_1216111..1216261_POSITIVE