Origin IGS: aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaacctattgttttgcattgtaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgctttcgcaatgccaaatcgaaattaccgttttctttcggaataatctttacaaaacggaagctgttttcgggatttgcctttggtaagaaaaaggaaatcgagttgcacaatattcttatgttgaacctaacgtttgtgttctttccgaggtgtggtaagttacatttccaaactttttatgttggatatacataaaacagaaaatagttcaaagttgtgctttcgctttggtgttatgaaatacttttattacctttgtacgaaagttcgtaacgagaagtttggctgttgcagttcgatagcaagccgtgattgacct Mask Tandem Repeat Region ================================================ aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnataggtttta Find is-nt database================================================ Query_seq: PI1803:PI1804|PI1803:PI1804:conserved hypothetical protein:hypothetical protein:->->:1737852..1738248 397 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1803:PI1804|PI1803:PI1804:conserved hypothetical protein:hypothetical protein:->->:1737852..1738248 397 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1803:PI1804|PI1803:PI1804:conserved hypothetical protein:hypothetical protein:->->:1737852..1738248 397 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 7 End: 108 ...... M T A C Y R T A T A K L L V T N F R T K V I K V F H N T K A K A Q L ................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 201 End: 395 ........................................................................................................................................................................................................ M R I L C N S I S F F L P K A N P E N S F R F V K I I P K E N G N F D L A L R K R L F C N A K P T L L P C K T A A F T M .. Protein_Len: 33 Strand: + Start: 298 End: 396 ......................................................................................................................................................................................................................................................................................................... M S I W H C E S G S F A T Q N L R F Y R A K Q P L L Q C K T I G F . Protein_Len: 60 Strand: - Start: 128 End: 388 ............................................................................................................................... N H L E I E K K K G F A F G S F L K R K T F I I G F S F P L K S K A N R F R S K Q L A F G V S K G H L V A A K V I C F M ......... Protein_Len: 60 Strand: - Start: 7 End: 186 ...... D R S A I S S C C G F K E N R V K T C L Y Y F Y K M V G F R F C L K S S N E T K H I D L M F L K S I Y S V V G R F S C M ................................................................................................................................................................................................................... aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 86 HP_score: -11.2 Tail_Score: -4.13767 Start: 320 End: 370 Full_Region: TTTGGCATTGCGAAA GCGGCTCTTTTGCAACGCAAAACC TAC GCTTTTACCGTGCAAAACAGCCGC TTTTACAATGCAAAA ...............................................................................................................................................................................................................................................................................................................................GCGGCTCTTTTGCAACGCAAAACCTACGCTTTTACCGTGCAAAACAGCCGC........................... TransTerm Strand: - Conf: 100 HP_score: -13.4 Tail_Score: -3.57144 Start: 320 End: 370 Full_Region: ttttgcattgtaaaa gcggctgttttgcacggtaaaagc gta ggttttgcgttgcaaaagagccgc tttcgcaatgccaaa ...............................................................................................................................................................................................................................................................................................................................gcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgc........................... Find igs database================================================ Query_seq: PI1803:PI1804|PI1803:PI1804:conserved hypothetical protein:hypothetical protein:->->:1737852..1738248 397 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Intra-Species Hit: Count: 29 Min: 1 Max: 397 Len: 397 Subject: pint_PI1803_PI1804|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1737852,1738248]|397 HSP 1 e-value: 0.0 bit: 787.0 Len: 397 Query Start:1 Query End:397 Subject Strand: POSITIVE Subject Start: 1 Subject End: 397 aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta aggtcaatcacggcttgctatcgaactgcaacagccaaacttctcgttacgaactttcgtacaaaggtaataaaagtatttcataacaccaaagcgaaagcacaactttgaactattttctgttttatgtatatccaacataaaaagtttggaaatgtaacttaccacacctcggaaagaacacaaacgttaggttcaacataagaatattgtgcaactcgatttcctttttcttaccaaaggcaaatcccgaaaacagcttccgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta Subject: pint_PI0694_PI0695|Na+/dicarboxylate or sulfate symporter:xanthine/uracil permease family protein|POSITIVE:POSITIVE|[631238,631552]|315 HSP 1 e-value: 1.0E-49 bit: 198.0 Len: 132 Query Start:265 Query End:396 Subject Strand: POSITIVE Subject Start: 87 Subject End: 218 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagataattccgacggaaaacggtaatttcgatttgacactgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI0574_PI0575|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[519505,519700]|196 HSP 1 e-value: 3.0E-47 bit: 190.0 Len: 132 Query Start:265 Query End:396 Subject Strand: POSITIVE Subject Start: 9 Subject End: 140 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagataattccgaagaaaaatgataatttcgatttgacgttgcgaaagtggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttgcaatgcaaaacaataggtttt. Subject: pint_PI0023_PI0024|internalin-related protein:hypothetical protein|POSITIVE:POSITIVE|[28131,28517]|387 HSP 1 e-value: 2.0E-45 bit: 184.0 Len: 109 Query Start:289 Query End:397 Subject Strand: POSITIVE Subject Start: 238 Subject End: 346 ................................................................................................................................................................................................................................................................................................aaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ................................................................................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta Subject: pint_PI0051_PI0054|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[53449,54718]|1270 HSP 1 e-value: 2.0E-44 bit: 180.0 Len: 131 Query Start:266 Query End:396 Subject Strand: NEGATIVE Subject Start: 88 Subject End: 218 .........................................................................................................................................................................................................................................................................ttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .........................................................................................................................................................................................................................................................................ttttgtaaagattatttctttagaaaatggtaatttcgatttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI2088_PI2089|conserved hypothetical protein; possible PAP2 superfamily protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2045876,2046339]|464 HSP 1 e-value: 2.0E-42 bit: 174.0 Len: 132 Query Start:266 Query End:397 Subject Strand: POSITIVE Subject Start: 155 Subject End: 286 .........................................................................................................................................................................................................................................................................ttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta .........................................................................................................................................................................................................................................................................ttttgtaaagataattcgttaagaaaacgataatttcgatttgacgttgcgaaagctgctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta Subject: pint_PI2051_PI2052|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2007896,2008733]|838 HSP 1 e-value: 2.0E-42 bit: 174.0 Len: 132 Query Start:265 Query End:396 Subject Strand: NEGATIVE Subject Start: 332 Subject End: 463 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagataattcgttaagaaaacgataatttcgatttgacgttgcgaaagcagctcttttgcaacgcaaaacctatgcttttaccgtgcaaaacagccgcttttaggatgcaaaacaataggtttt. Subject: pint_PI1563_PI1564|thiol:disulfide interchange protein dsbD:surface antigen BspA|POSITIVE:POSITIVE|[1482876,1483594]|719 HSP 1 e-value: 6.0E-39 bit: 163.0 Len: 130 Query Start:267 Query End:396 Subject Strand: NEGATIVE Subject Start: 387 Subject End: 516 ..........................................................................................................................................................................................................................................................................tttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ..........................................................................................................................................................................................................................................................................tttgtaaagataattctgatgaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI1577_PI1578|conserved hypothetical protein:rod shape-determining protein|POSITIVE:POSITIVE|[1503940,1504327]|388 HSP 1 e-value: 6.0E-36 bit: 153.0 Len: 129 Query Start:269 Query End:397 Subject Strand: POSITIVE Subject Start: 72 Subject End: 200 ............................................................................................................................................................................................................................................................................tgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ............................................................................................................................................................................................................................................................................tgtaaagataattcattaagaaaactataatttcgatttgacgttgcgaaagcggctcttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta Subject: pint_PI1332_PI1333|aminotransferase, possible pyridoxal-phosphate-dependent aminotransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1250627,1250916]|290 HSP 1 e-value: 6.0E-36 bit: 153.0 Len: 101 Query Start:296 Query End:396 Subject Strand: POSITIVE Subject Start: 121 Subject End: 221 .......................................................................................................................................................................................................................................................................................................taatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .......................................................................................................................................................................................................................................................................................................taatttcgatttgacattgcgaaaacggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggagtgcaaaacaataggtttt. Subject: pint_PI1930_PI1931|conserved hypothetical protein; possible ankyrin repeat protein (eukaryotic-like):alpha glycan phosphorylase, maltodextrin phosphorylase|POSITIVE:POSITIVE|[1875115,1875595]|481 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 108 Query Start:289 Query End:396 Subject Strand: POSITIVE Subject Start: 136 Subject End: 243 ................................................................................................................................................................................................................................................................................................aaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ................................................................................................................................................................................................................................................................................................aaaatggtaatttcgatttgacgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtttt. Subject: pint_PI1051_PI1052|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[970289,970631]|343 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 132 Query Start:265 Query End:396 Subject Strand: NEGATIVE Subject Start: 41 Subject End: 172 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagataattctgatgaaaaacaataatttcgttttgacgttgcgaaagcggctcttttacaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI1391_PI1392|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1311755,1312040]|286 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 133 Query Start:264 Query End:396 Subject Strand: NEGATIVE Subject Start: 57 Subject End: 189 .......................................................................................................................................................................................................................................................................cgttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .......................................................................................................................................................................................................................................................................cgttttgtaaagataattccgacgaaaaacggtaatttcgatttggcgttgcgaaagcggctgttttgcagcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt. Subject: pint_PI0680_PI0682|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[618694,619111]|418 HSP 1 e-value: 5.0E-33 bit: 143.0 Len: 132 Query Start:265 Query End:396 Subject Strand: POSITIVE Subject Start: 113 Subject End: 244 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagattattctgttaaaaagcgataactgcgctttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttagcgtgcaaaacagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI2056_PI2057|conserved hypothetical protein:conserved hypothetical protein; possible metal-dependent membrane protease|POSITIVE:POSITIVE|[2014489,2015078]|590 HSP 1 e-value: 2.0E-32 bit: 141.0 Len: 131 Query Start:266 Query End:396 Subject Strand: POSITIVE Subject Start: 341 Subject End: 471 .........................................................................................................................................................................................................................................................................ttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .........................................................................................................................................................................................................................................................................ttttgtaaagataattcgttaagaaaacaataatttcgatttgacgttgcgaaagcggctcttttgtaatgcaaaacttacgcttttaccatgcaaaacagccgcttttggaatgcgaaacaataggtttt. Subject: pint_PI1424_PI1425|probable peptidase family M48:conserved hypothetical protein|POSITIVE:POSITIVE|[1344105,1344627]|523 HSP 1 e-value: 3.0E-28 bit: 127.0 Len: 132 Query Start:265 Query End:396 Subject Strand: NEGATIVE Subject Start: 107 Subject End: 238 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagattattcgtaagaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacagccgcttttgccgtgtaaaacctacgcttttggaatgcaaaacaataggtttt. Subject: pint_PI0948_PI0949|hypothetical protein:endonuclease|POSITIVE:POSITIVE|[882039, 882403]|365 HSP 1 e-value: 3.0E-28 bit: 127.0 Len: 132 Query Start:265 Query End:396 Subject Strand: POSITIVE Subject Start: 140 Subject End: 271 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaggataattcgtaaggaaaatgataatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgctttgaccgtgtaaaacctacgcttttacaatgcaaaacaataggtttt. Subject: pint_PI1583_PI1584|phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR)/IMP cyclohydrolase (ATIC):A/G-specific adenine glycosylase|POSITIVE:POSITIVE|[1511261,1512037]|777 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 101 Query Start:296 Query End:396 Subject Strand: NEGATIVE Subject Start: 411 Subject End: 511 .......................................................................................................................................................................................................................................................................................................taatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .......................................................................................................................................................................................................................................................................................................taatttcgttttgacgttgcgaaagcggctgttttgcgaggtaaaacctacgcttttgccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt. Subject: pint_PI0195_PI0196|epimerase/reductase:hypothetical protein|POSITIVE:POSITIVE|[188263,188574]|312 HSP 1 e-value: 8.0E-26 bit: 119.0 Len: 100 Query Start:297 Query End:396 Subject Strand: POSITIVE Subject Start: 176 Subject End: 275 ........................................................................................................................................................................................................................................................................................................aatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt. Subject: pint_PI1688_PI1689|possible GTPase:conserved hypothetical protein|POSITIVE:POSITIVE|[1603964,1604449]|486 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 132 Query Start:266 Query End:397 Subject Strand: NEGATIVE Subject Start: 173 Subject End: 304 .........................................................................................................................................................................................................................................................................ttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta .........................................................................................................................................................................................................................................................................ttttgtaaagataattcggagggaaaatgataatttcgatttgacgttgcgaaagcgtatgttttgcgatgcaaaagagccgcttttaccgtgcaaaacctacgcttttacaatgcaaaacaataggtttta HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:317 Query End:351 Subject Strand: POSITIVE Subject Start: 219 Subject End: 253 ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................. ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcatcgcaaaacatacgcttt.............................................. Subject: pint_PI1915_PI1916|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1859967,1860350]|384 HSP 1 e-value: 7.0E-20 bit: 99.6 Len: 109 Query Start:289 Query End:397 Subject Strand: POSITIVE Subject Start: 137 Subject End: 245 ................................................................................................................................................................................................................................................................................................aaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaa-cctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ................................................................................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcggctgttttgcacggtaaaagcgtatg-ttttacgttgcaaaacagccgcttttggaatgcaaaacaataggtttta HSP 2 e-value: 7.0E-17 bit: 89.7 Len: 57 Query Start:317 Query End:373 Subject Strand: NEGATIVE Subject Start: 165 Subject End: 221 ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttt........................ ............................................................................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgtaaaacatacgcttttaccgtgcaaaacagccgcttt........................ Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 7.0E-20 bit: 99.6 Len: 102 Query Start:296 Query End:397 Subject Strand: POSITIVE Subject Start: 771 Subject End: 872 .......................................................................................................................................................................................................................................................................................................taatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta .......................................................................................................................................................................................................................................................................................................taatttcgatttggcgttgcgaaagcgtaggttttgcaacgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttta Subject: pint_PI1981_PI1982|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1938199,1938578]|380 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 133 Query Start:265 Query End:397 Subject Strand: POSITIVE Subject Start: 143 Subject End: 275 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ........................................................................................................................................................................................................................................................................gttttgtaaagataattctgataaaaaacagcaatttcggtttgacattgcgaaagcggctgttttgcatttcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggtttta Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 109 Query Start:289 Query End:397 Subject Strand: NEGATIVE Subject Start: 232 Subject End: 340 ................................................................................................................................................................................................................................................................................................aaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ................................................................................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaacctacacttttggaatgcaaaacaataggtttta Subject: pint_PI1958_PI1959|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1917430,1917847]|418 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 132 Query Start:266 Query End:397 Subject Strand: POSITIVE Subject Start: 182 Subject End: 313 .........................................................................................................................................................................................................................................................................ttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta .........................................................................................................................................................................................................................................................................ttttgtaaagataattctgataaaaaacagcaatttcggtttgacattgcaaaagcggctgttttgcattgcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggtttta Subject: pint_PI1728_PI1729|ATP-dependent Clp protease, proteolytic subunit:FKBP-type peptidyl-prolyl cis-trans isomerase, trigger factor|POSITIVE:POSITIVE|[1647719,1648555]|837 HSP 1 e-value: 7.0E-17 bit: 89.7 Len: 133 Query Start:265 Query End:397 Subject Strand: NEGATIVE Subject Start: 415 Subject End: 547 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta ........................................................................................................................................................................................................................................................................gttttgtaaagataattcgttgagaaaacggtaatttcgatttgacgttgcaaaagcgtaggttttgcaaggcaaaagagccgcttttaccgtgcaaaagcggctcttttacaatgcgaaacaataggtttta Subject: pint_PI0945_PI0946|sodium:solute symporter family protein:glutathione peroxidase|POSITIVE:POSITIVE|[877685,878112]|428 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 103 Query Start:294 Query End:396 Subject Strand: POSITIVE Subject Start: 107 Subject End: 209 .....................................................................................................................................................................................................................................................................................................ggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .....................................................................................................................................................................................................................................................................................................ggtaattgcgctttggcattgcgaaagcggctgttttgcgatgcaaaagagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggtttt. Subject: pint_PI1441_PI1442|hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme)|POSITIVE:POSITIVE|[1363734,1364088]|355 HSP 1 e-value: 7.0E-14 bit: 79.8 Len: 132 Query Start:265 Query End:396 Subject Strand: NEGATIVE Subject Start: 86 Subject End: 217 ........................................................................................................................................................................................................................................................................gttttgtaaagattattccgaaagaaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. ........................................................................................................................................................................................................................................................................gttttgtaaagataatttacaaggaaaacggtattttcgatttgacgttgcgaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggtttt. HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:317 Query End:351 Subject Strand: POSITIVE Subject Start: 131 Subject End: 165 ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................. ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................. Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 6.0E-11 bit: 69.9 Len: 35 Query Start:317 Query End:351 Subject Strand: NEGATIVE Subject Start: 195 Subject End: 229 ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................. ............................................................................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................. HSP 2 e-value: 1.0E-9 bit: 65.9 Len: 101 Query Start:296 Query End:396 Subject Strand: POSITIVE Subject Start: 174 Subject End: 274 .......................................................................................................................................................................................................................................................................................................taatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt. .......................................................................................................................................................................................................................................................................................................taatttcgatttgacattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGUCAAUCACGGCUUGCUAUCGAACUGCAACAGCCAAACUUCUCGUUACGAACUUUCGUACAAAGGUAAUAAAAGUAUUUCAUAACACCAAAGCGAAAGCACAACUUUGAACUAUUUUCUGUUUUAUGUAUAUCCAACAUAAAAAGUUUGGAAAUGUAACUUACCACACCUCGGAAAGAACACAAACGUUAGGUUCAACAUAAGAAUAUUGUGCAACUCGAUUUCCUUUUUCUUACCAAAGGCAAAUCCCGAAAACAGCUUCCGUUUUGUAAAGAUUAUUCCGAAAGAAAACGGUAAUUUCGAUUUGGCAUUGCGAAAGCGGCUCUUUUGCAACGCAAAACCUACGCUUUUACCGUGCAAAACAGCCGCUUUUACAAUGCAAAACAAUAGGUUUUA .((........(((((((.........)))..)))).........((((.(((((((..(((....)))...)))))...)).)))).))...((....))......(((((((........((((((((.......)))))))).(((((....(((..(((.((.......)).))).))))))))....)))))))..(((((.((((((.......((((((((((........))))).))))).(((((.......(((((((.(...((....))....).)))))))...))))).....((((((..(((((((((.(((((((.((.((((.......))))..))))))))).)))))))))..))))))...))))))..))))) (-91.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACCUAUUGUUUUGCAUUGUAAAAGCGGCUGUUUUGCACGGUAAAAGCGUAGGUUUUGCGUUGCAAAAGAGCCGCUUUCGCAAUGCCAAAUCGAAAUUACCGUUUUCUUUCGGAAUAAUCUUUACAAAACGGAAGCUGUUUUCGGGAUUUGCCUUUGGUAAGAAAAAGGAAAUCGAGUUGCACAAUAUUCUUAUGUUGAACCUAACGUUUGUGUUCUUUCCGAGGUGUGGUAAGUUACAUUUCCAAACUUUUUAUGUUGGAUAUACAUAAAACAGAAAAUAGUUCAAAGUUGUGCUUUCGCUUUGGUGUUAUGAAAUACUUUUAUUACCUUUGUACGAAAGUUCGUAACGAGAAGUUUGGCUGUUGCAGUUCGAUAGCAAGCCGUGAUUGACCU ...........((((.((((((..(((((((((.(((((((((...(((((....))))).)).))))))).)))))))))..)))))).))))...((((((.((((.((.(((((.........((((....(((.((((((((..(((((.(((((........))))))))))..((((..((((((....))))))....)))).(((((((....(((((.(((....(((((.........)))))..))).)))))...)))))))...)))))))))))....))))(((...(((((..((((((((...((((((..(((....))).)))))))))))))).)))))..)))........))))).)).)))).))))))..... (-112.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 996 370 321 204 155 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 944 370 321 73 24 pint:PI1829|5end_hypothetical_protein_1769255..1769485_POSITIVE 922 370 321 73 24 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 922 370 321 199 150 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 916 370 321 73 24 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 914 360 311 51 2 pint:PI2144|5end_hypothetical_protein_2091954..2092184_POSITIVE 893 370 321 73 24 pint:PI1567|5end_hypothetical_protein_1489229..1489459_POSITIVE 892 360 311 205 156 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 887 370 321 73 24 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 887 370 321 61 12 pint:PI1129|5end_hypothetical_protein_1055377..1055607_POSITIVE 865 340 291 227 178 pint:PI1830|5end_conserved_hypothetical_protein_1769439..1769669_POSITIVE 853 370 322 100 52 pint:PI1604|5end_hypothetical_protein_1534017..1534247_POSITIVE 844 360 312 224 176 pint:PI2003|5end_hypothetical_protein_1960823..1961053_POSITIVE 834 370 321 73 24 pint:PI1601|5end_hypothetical_protein_1530282..1530512_POSITIVE 813 370 321 73 24 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 813 370 321 122 73 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 810 370 321 73 24 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 785 350 301 53 4 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 785 350 301 61 12 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 785 350 301 53 4 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 968 350 301 135 86 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 925 360 312 119 71 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 887 370 321 56 7 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 877 360 312 145 97 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 865 340 291 147 98 pint:PI1829|3end_hypothetical_protein_1769439..1769589_POSITIVE 858 370 321 115 66 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 857 360 311 145 96 pint:PI0061|3end_hypothetical_protein_60690..60840_POSITIVE 820 340 291 146 97 pint:PI1905|3end_hypothetical_protein_1847854..1848004_POSITIVE 813 370 321 56 7 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 786 370 321 115 66 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 785 350 301 135 86 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 779 356 312 151 107 pint:PI1076|3end_hypothetical_protein_999065..999215_POSITIVE 777 360 311 145 96 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 770 360 312 149 101 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 735 390 343 48 1 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 735 390 343 48 1 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 702 360 311 145 96 pint:PI0004|3end_hypothetical_protein_6389..6539_POSITIVE 697 356 312 151 107 pint:PI1601|3end_hypothetical_protein_1530454..1530604_POSITIVE 696 350 305 149 104 pint:PI1528|3end_hypothetical_protein_1449878..1450028_POSITIVE 693 330 282 115 70 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE