Origin IGS: gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagcccttttgtacgtttgtgttttaatgaggttcttaagcaattggcttcggactattacgcaaacaaacagaaaagaatacagaaagaatgaataagacttatagaatattaagcctgttcttgcttacactggttgcttctgcagcgatggcgcaagacaagaaagatattttcaatccagtaaaccacgccgttacgtcgcagatgatagcacccgatgctcgtgcagcaggtatgggagac Mask Tandem Repeat Region ================================================ gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Find is-nt database================================================ Query_seq: PI1794:PI1795|PI1794:PI1795:conserved hypothetical protein:conserved hypothetical protein:->->:1726757..1727003 247 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1794:PI1795|PI1794:PI1795:conserved hypothetical protein:conserved hypothetical protein:->->:1726757..1727003 247 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1794:PI1795|PI1794:PI1795:conserved hypothetical protein:conserved hypothetical protein:->->:1726757..1727003 247 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 25 End: 132 ........................ M G C Y H L R R N G V V Y W I E N I F L V L R H R C R S N Q C K Q E Q A ................................................................................................................... Protein_Len: 45 Strand: + Start: 8 End: 142 ....... M P A A R A S G A I I C D V T A W F T G L K I S F L S C A I A A E A T S V S K N R L N I L ......................................................................................................... Protein_Len: 37 Strand: + Start: 135 End: 245 ...................................................................................................................................... M F Y K S Y S F F L Y S F L F V C V I V R S Q L L K N L I K T Q T Y K R A .. Protein_Len: 45 Strand: - Start: 2 End: 136 . R G Y R S C S C R T S D D A V Y R R P K S S Q F Y R E Q R A G D S C F C G T Y A L V P K M ............................................................................................................... Protein_Len: 60 Strand: - Start: 1 End: 204 D P A I M Q S T V A H N V P N F I D K K D Q A M A A S A V L T L L F L S L I R Y T K N M R E T N K E T Q K R L L G F G M ........................................... gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1794:PI1795|PI1794:PI1795:conserved hypothetical protein:conserved hypothetical protein:->->:1726757..1727003 247 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Intra-Species Hit: Count: 1 Min: 1 Max: 247 Len: 247 Subject: pint_PI1794_PI1795|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1726757,1727003]|247 HSP 1 e-value: 1.0E-137 bit: 490.0 Len: 247 Query Start:1 Query End:247 Subject Strand: POSITIVE Subject Start: 1 Subject End: 247 gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta gtctcccatacctgctgcacgagcatcgggtgctatcatctgcgacgtaacggcgtggtttactggattgaaaatatctttcttgtcttgcgccatcgctgcagaagcaaccagtgtaagcaagaacaggcttaatattctataagtcttattcattctttctgtattcttttctgtttgtttgcgtaatagtccgaagccaattgcttaagaacctcattaaaacacaaacgtacaaaagggctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCUCCCAUACCUGCUGCACGAGCAUCGGGUGCUAUCAUCUGCGACGUAACGGCGUGGUUUACUGGAUUGAAAAUAUCUUUCUUGUCUUGCGCCAUCGCUGCAGAAGCAACCAGUGUAAGCAAGAACAGGCUUAAUAUUCUAUAAGUCUUAUUCAUUCUUUCUGUAUUCUUUUCUGUUUGUUUGCGUAAUAGUCCGAAGCCAAUUGCUUAAGAACCUCAUUAAAACACAAACGUACAAAAGGGCUUA ....(((.((((((.(((....))).))))))........((((....(((((...((..(((.(((.((((.......((((((.(((((((....(((.....))).....))))))))))))).(((((((........)))))))..)))).)))....)))..))...)))))(((((.........((..((((.....))))..)).........)))))....)))).....))).... (-58.27) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCCUUUUGUACGUUUGUGUUUUAAUGAGGUUCUUAAGCAAUUGGCUUCGGACUAUUACGCAAACAAACAGAAAAGAAUACAGAAAGAAUGAAUAAGACUUAUAGAAUAUUAAGCCUGUUCUUGCUUACACUGGUUGCUUCUGCAGCGAUGGCGCAAGACAAGAAAGAUAUUUUCAAUCCAGUAAACCACGCCGUUACGUCGCAGAUGAUAGCACCCGAUGCUCGUGCAGCAGGUAUGGGAGAC (((((..((((((..((((((((..((.((((((...(((....)))))))))...))..))))))))..)))))).................(((((.(.((((........))))).)))))..)))))...(((.(((((((((.(((((((((....((..((..((.....))..))..))......))))))..))).)))))....)))).)))...(((((((.....))))))).... (-60.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 395 60 13 76 31 pint:PI0997|5end_hypothetical_protein_920975..921205_POSITIVE 393 58 17 174 136 pint:PI2034|5end_conserved_hypothetical_protein_1987573..1987803_POSITIVE 393 56 14 222 171 pint:PI0167|5end_hypothetical_protein_163164..163394_POSITIVE 391 45 3 65 20 pint:PI0139|5end_pyridoxine_biosynthesis_protein_131835..132065_POSITIVE 380 60 14 106 57 pint:PI1820|5end_hypothetical_protein_1759962..1760192_POSITIVE 373 58 12 59 10 pint:PI1618|5end_hypothetical_protein_1542670..1542900_POSITIVE 369 58 11 217 162 pint:PI2091|5end_DNA_processing_protein_2048019..2048249_POSITIVE 364 130 84 66 21 pint:PI0019|5end_conserved_hypothetical_protein_23742..23972_POSITIVE 355 99 51 200 149 pint:PI2102|5end_DNA_topoisomerase_IV,_subunit_B_2055706..2055936_POSITIVE 355 58 11 119 55 pint:PI0945|5end_sodium:solute_symporter_family_protein_876249..876479_POSITIVE 350 130 89 105 48 pint:PI0750|5end_zinc_protease_677746..677976_POSITIVE 349 59 20 40 1 pint:PI1925|5end_conserved_hypothetical_protein_1866387..1866617_POSITIVE 349 129 83 64 12 pint:PI1810|5end_outer_membrane_protein_1748305..1748535_POSITIVE 348 129 81 48 1 pint:PI1011|5end_cytochrome_c_biogenesis_protein_CycY_932115..932345_POSITIVE 347 126 83 177 129 pint:PI1285|5end_ATP_synthase,_B_subunit_1200348..1200578_POSITIVE 347 59 12 107 44 pint:PI0734|5end_ABC_transporter,_ATP-binding_/_permease_667052..667282_POSITIVE 345 54 13 66 18 pint:PI1890|5end_cell_division_protein_FtsW_1832886..1833116_POSITIVE 344 62 23 90 54 pint:PI0151|5end_ISPg4-related_transposase_(IS5_family)_143805..144035_POSITIVE 343 60 12 228 154 pint:PI1685|5end_GAF_domain-containing_protein_1601712..1601942_POSITIVE 343 60 12 110 36 pint:PI1686|5end_hypothetical_protein_1601594..1601824_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 60 11 54 6 pint:PI0418|3end_hypothetical_protein_386856..387006_POSITIVE 391 59 13 68 19 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 376 60 11 73 25 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 370 58 11 101 43 pint:PI0042|3end_hypothetical_protein_43444..43594_POSITIVE 348 129 81 48 1 pint:PI1010|3end_conserved_hypothetical_protein_932195..932345_POSITIVE 347 126 83 72 24 pint:PI1284|3end_ATP_synthase,_delta_subunit_1200323..1200473_POSITIVE 345 60 12 111 64 pint:PI1623|3end_hypothetical_protein_1549261..1549411_POSITIVE 345 60 12 141 94 pint:PI0331|3end_hypothetical_protein_312575..312725_POSITIVE 345 60 12 111 64 pint:PI0330|3end_hypothetical_protein_312545..312695_POSITIVE 344 62 23 38 2 pint:PI0150|3end_hypothetical_protein_143833..143983_POSITIVE 337 56 11 146 103 pint:PI2045|3end_hypothetical_protein_1998723..1998873_POSITIVE 336 60 13 114 68 pint:PI0125|3end_undecaprenyl_pyrophosphate_synthase_117890..118040_POSITIVE 331 59 11 141 87 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 328 124 88 53 17 pint:PI0632|3end_NusB_family_protein_(N_utilization_substance_protein_B)_579731..579881_POSITIVE 328 56 12 149 94 pint:PI0251|3end_hypothetical_protein_247810..247960_POSITIVE 326 59 12 137 82 pint:PI1655|3end_hypothetical_protein_1570217..1570367_POSITIVE 326 56 13 83 39 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 322 63 24 47 6 pint:PI1457|3end_N-acetylmuramoyl-L-alanine_amidase_1381228..1381378_POSITIVE 321 55 15 118 67 pint:PI0624|3end_hypothetical_protein_574471..574621_POSITIVE 320 55 13 136 92 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE