Origin IGS: taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcctacataccatgttgtgggttcgagcttctttatctctatctttgcgtttatgctgatagttagaagacagagcgagaacaataaagttaatcgtttcatttttattgttta Mask Tandem Repeat Region ================================================ taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Find is-nt database================================================ Query_seq: PI1772:PI1773|PI1772:PI1773:pullulanase:alpha-amylase (neopullulanase):->->:1704308..1704421 114 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1772:PI1773|PI1772:PI1773:pullulanase:alpha-amylase (neopullulanase):->->:1704308..1704421 114 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1772:PI1773|PI1772:PI1773:pullulanase:alpha-amylase (neopullulanase):->->:1704308..1704421 114 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 21 End: 113 .................... M N F I V L A L S S N Y Q H K R K D R D K E A R T H N M V C R . Protein_Len: 36 Strand: + Start: 7 End: 114 ...... M K M K R L T L L F S L C L L T I S I N A K I E I K K L E P T T W Y V G Protein_Len: 32 Strand: - Start: 3 End: 98 .. V I F I F R N V K N N E S Q R R V I L M F A F I S I F F S S G M ................ taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1772:PI1773|PI1772:PI1773:pullulanase:alpha-amylase (neopullulanase):->->:1704308..1704421 114 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: pint_PI1772_PI1773|pullulanase:alpha-amylase (neopullulanase)|POSITIVE:POSITIVE|[1704308,1704421]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga taaacaataaaaatgaaacgattaactttattgttctcgctctgtcttctaactatcagcataaacgcaaagatagagataaagaagctcgaacccacaacatggtatgtagga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAAUAAAAAUGAAACGAUUAACUUUAUUGUUCUCGCUCUGUCUUCUAACUAUCAGCAUAAACGCAAAGAUAGAGAUAAAGAAGCUCGAACCCACAACAUGGUAUGUAGGA ..(((((((((..(((.....)))..)))))))))...(((((....(((..(((((.((......))...))))))))...)).))).....((.(((........))).)). (-17.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCUACAUACCAUGUUGUGGGUUCGAGCUUCUUUAUCUCUAUCUUUGCGUUUAUGCUGAUAGUUAGAAGACAGAGCGAGAACAAUAAAGUUAAUCGUUUCAUUUUUAUUGUUUA .((((((........)))))).(((..(((((((....(((((...(((....))).)))))...))))).))..)))(((((((((((.............))))))))))). (-27.52) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 277 105 72 188 150 pint:PI2087|5end_dihydropicolinate_reductase_2043423..2043653_POSITIVE 276 80 32 90 37 pint:PI0584|5end_hypothetical_protein_527352..527582_POSITIVE 253 100 59 144 103 pint:PI1008|5end_cytochrome_c_nitrite_reductase,_small_subunit_928784..929014_POSITIVE 253 69 31 195 154 pint:PI0869|5end_possible_exodeoxyribonuclease_VII_(small_subunit)_805518..805748_POSITIVE 246 90 43 73 29 pint:PI2067|5end_hypothetical_protein_2027760..2027990_POSITIVE 246 94 54 199 148 pint:PI1953|5end_possible_ABC-type_permease_1907135..1907365_POSITIVE 243 110 84 98 77 pint:PI2068|5end_hypothetical_protein_2028328..2028558_POSITIVE 242 79 34 124 84 pint:PI0868|5end_exodeoxyribonuclease_VII,_large_subunit_804201..804431_POSITIVE 239 68 26 59 1 pint:PI0740|5end_hypothetical_protein_672112..672342_POSITIVE 235 97 56 81 27 pint:PI0302|5end_hypothetical_protein_286325..286555_POSITIVE 234 68 26 197 150 pint:PI1980|5end_conserved_hypothetical_protein_1937470..1937700_POSITIVE 234 80 34 98 53 pint:PI1897|5end_conserved_hypothetical_protein_1840619..1840849_POSITIVE 230 76 38 211 170 pint:PI1981|5end_hypothetical_protein_1937897..1938127_POSITIVE 230 107 63 200 148 pint:PI1536|5end_hypothetical_protein_1455555..1455785_POSITIVE 229 58 13 51 5 pint:PI1004|5end_conserved_hypothetical_protein_925820..926050_POSITIVE 229 75 37 80 51 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 228 53 34 223 204 pint:PI1726|5end_ATP-dependent_DNA_helicase_1643402..1643632_POSITIVE 226 59 11 213 158 pint:PI0127|5end_conserved_hypothetical_protein_118683..118913_POSITIVE 223 99 55 111 63 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 220 75 31 121 74 pint:PI0489|5end_probable_cardiolipin_synthetase_447610..447840_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 277 105 72 135 97 pint:PI2086|3end_signal_peptidase_I_2043450..2043600_POSITIVE 256 54 30 70 42 pint:PI2122|3end_conserved_hypothetical_protein_2070480..2070630_POSITIVE 243 110 84 27 6 pint:PI2067|3end_hypothetical_protein_2028337..2028487_POSITIVE 242 79 34 45 5 pint:PI0867|3end_hypothetical_protein_804202..804352_POSITIVE 239 106 64 53 8 pint:PI2166|3end_hypothetical_protein_2112073..2112223_POSITIVE 235 64 24 109 73 pint:PI0787|3end_glycosyltransferase_725793..725943_POSITIVE 234 80 34 62 17 pint:PI1895|3end_penicillin-binding_protein_2_1840663..1840813_POSITIVE 230 107 63 66 14 pint:PI1535|3end_response_regulator_1455501..1455651_POSITIVE 229 106 63 66 31 pint:PI2083|3end_hypothetical_protein_2040661..2040811_POSITIVE 222 52 13 76 34 pint:PI1941|3end_outer_membrane_receptor;_ragA_protein_1900619..1900769_POSITIVE 218 95 53 78 24 pint:PI0425|3end_hypothetical_protein_389722..389872_POSITIVE 217 107 61 87 36 pint:PI0168|3end_methionyl-tRNA_synthetase_(methionine--tRNA_ligase)_163279..163429_POSITIVE 215 78 39 67 33 pint:PI0341|3end_conserved_hypothetical_protein_317615..317765_POSITIVE 214 60 14 78 32 pint:PI0356|3end_hypothetical_protein_327288..327438_POSITIVE 211 89 46 98 55 pint:PI0282|3end_hypothetical_protein_266826..266976_POSITIVE 210 69 29 118 73 pint:PI0962|3end_phosphoglycerate_mutase_892702..892852_POSITIVE 209 50 12 133 100 pint:PI0546|3end_hypothetical_protein_498238..498388_POSITIVE 208 80 32 83 43 pint:PI1555|3end_conserved_hypothetical_protein_1471754..1471904_POSITIVE 205 93 58 141 109 pint:PI0020|3end_conserved_hypothetical_protein_24955..25105_POSITIVE 204 99 68 151 117 pint:PI1892|3end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_1835547..1835697_POSITIVE