Origin IGS: attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttacacaatatctacgcttatgaaaaatatttacagctttatgggcttcttttcgttcatagagcgagttattcccattaaacttctacatttccatttttacattcttccaacaagtccgtgtagcttttcgccaat Mask Tandem Repeat Region ================================================ attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Find is-nt database================================================ Query_seq: PI1732:PI1733|PI1732:PI1733:hypothetical protein:hypothetical protein:->->:1652159..1652299 141 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1732:PI1733|PI1732:PI1733:hypothetical protein:hypothetical protein:->->:1652159..1652299 141 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1732:PI1733|PI1732:PI1733:hypothetical protein:hypothetical protein:->->:1652159..1652299 141 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: - Start: 3 End: 131 .. P S F S C P S T P L I Y F H F H L L K I P I V R E I F S F F G M F S Y I N K M L T S M .......... attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1732:PI1733|PI1732:PI1733:hypothetical protein:hypothetical protein:->->:1652159..1652299 141 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Intra-Species Hit: Count: 1 Min: 1 Max: 141 Len: 141 Subject: pint_PI1732_PI1733|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1652159,1652299]|141 HSP 1 e-value: 1.0E-74 bit: 280.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta attggcgaaaagctacacggacttgttggaagaatgtaaaaatggaaatgtagaagtttaatgggaataactcgctctatgaacgaaaagaagcccataaagctgtaaatatttttcataagcgtagatattgtgtaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGGCGAAAAGCUACACGGACUUGUUGGAAGAAUGUAAAAAUGGAAAUGUAGAAGUUUAAUGGGAAUAACUCGCUCUAUGAACGAAAAGAAGCCCAUAAAGCUGUAAAUAUUUUUCAUAAGCGUAGAUAUUGUGUAACUA ..........((.(((((((.((((((.(((((((((....((....)).....(((((.(((((......(((.((...)).))).......))))).)))))....)))))))))...)))).))...))))))).)). (-27.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUACACAAUAUCUACGCUUAUGAAAAAUAUUUACAGCUUUAUGGGCUUCUUUUCGUUCAUAGAGCGAGUUAUUCCCAUUAAACUUCUACAUUUCCAUUUUUACAUUCUUCCAACAAGUCCGUGUAGCUUUUCGCCAAU .((((((((.............(((((((((.......(((((((((((........))))))))))).((((..........))))...........)))))).)))...............)))))))).......... (-23.49) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 501 127 83 230 186 pint:PI1206|5end_ATP-dependent_DNA_helicase,_UvrD/PcrA/Rep_family_1124698..1124928_POSITIVE 463 126 81 167 122 pint:PI1272|5end_possible_transcriptional_regulator_1191702..1191932_POSITIVE 446 126 81 92 47 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 399 126 84 230 188 pint:PI0682|5end_hypothetical_protein_618972..619202_POSITIVE 396 139 91 199 148 pint:PI0660|5end_conserved_hypothetical_protein_603438..603668_POSITIVE 390 138 92 215 169 pint:PI1253|5end_hypothetical_protein_1175516..1175746_POSITIVE 387 109 62 181 134 pint:PI0828|5end_GMP_synthase_(glutamine_amidotransferase)_764077..764307_POSITIVE 387 109 62 95 48 pint:PI0827|5end_hypothetical_protein_763991..764221_POSITIVE 373 140 99 176 136 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 356 140 91 139 93 pint:PI1333|5end_conserved_hypothetical_protein_1250777..1251007_POSITIVE 354 120 71 223 169 pint:PI0286|5end_ISPg3-related_transposase_271833..272063_POSITIVE 347 136 100 231 194 pint:PI1456|5end_conserved_hypothetical_protein_1378864..1379094_POSITIVE 344 126 81 98 53 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 338 138 104 150 116 pint:PI2024|5end_hypothetical_protein_1978804..1979034_POSITIVE 334 90 42 231 183 pint:PI1438|5end_penicillin_tolerance_protein/steroid_biosynthesis_1356263..1356493_POSITIVE 334 127 83 93 45 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 325 140 93 225 179 pint:PI2030|5end_hypothetical_protein_1985601..1985831_POSITIVE 319 139 92 163 116 pint:PI0949|5end_endonuclease_882264..882494_POSITIVE 303 140 100 41 1 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 302 140 91 206 157 pint:PI0324|5end_conserved_hypothetical_protein_303735..303965_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 549 140 91 100 52 pint:PI1204|3end_hypothetical_protein_1124635..1124785_POSITIVE 442 117 71 147 102 pint:PI1436|3end_hypothetical_protein_1356232..1356382_POSITIVE 396 139 91 77 26 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 390 138 92 59 13 pint:PI1252|3end_hypothetical_protein_1175440..1175590_POSITIVE 387 109 62 69 22 pint:PI0826|3end_hypothetical_protein_764045..764195_POSITIVE 373 140 99 61 21 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 338 138 104 98 64 pint:PI2023|3end_hypothetical_protein_1978832..1978982_POSITIVE 324 140 99 86 46 pint:PI1072|3end_hypothetical_protein_990503..990653_POSITIVE 303 140 100 148 108 pint:PI1371|3end_hypothetical_protein_1292546..1292696_POSITIVE 299 49 3 62 14 pint:PI1522|3end_thioredoxin_M_1442037..1442187_POSITIVE 289 126 84 147 106 pint:PI0037|3end_hypothetical_protein_39616..39766_POSITIVE 278 139 104 72 37 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 273 121 81 54 13 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 271 139 101 99 61 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 271 140 104 89 53 pint:PI0902|3end_hypothetical_protein_832580..832730_POSITIVE 268 126 81 47 2 pint:PI0568|3end_hypothetical_protein_513131..513281_POSITIVE 261 139 92 133 86 pint:PI1786|3end_hypothetical_protein_1718380..1718530_POSITIVE 259 125 84 48 5 pint:PI0081|3end_hypothetical_protein_80700..80850_POSITIVE 258 124 84 57 17 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 249 46 3 147 99 pint:PI1818|3end_hypothetical_protein_1757270..1757420_POSITIVE