Origin IGS: tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaggaactttcaaccgctcttcccacttggaaagggcggtttctttttgcccttgtcacccagctttatcggtaaaacacaacggcaatgtaggtagcatacgggtagtttctcgctccgtcttttaacatttgaaaaatgagctaatatttccgtgaaacaataaaaacagcattgaaaaaccaaaaattttatttccgtttttcaaaagcagcggttttgaaaggtaaaacaataggttttgcgcggtgaaagcta Mask Tandem Repeat Region ================================================ tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Find is-nt database================================================ Query_seq: PI1725:PI1726|PI1725:PI1726:hypothetical protein:ATP-dependent DNA helicase:->->:1643281..1643541 261 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1725:PI1726|PI1725:PI1726:hypothetical protein:ATP-dependent DNA helicase:->->:1643281..1643541 261 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1725:PI1726|PI1725:PI1726:hypothetical protein:ATP-dependent DNA helicase:->->:1643281..1643541 261 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 24 End: 131 ....................... M F Y L S K P L L L K N G N K I F G F S M L F L L F H G N I S S F F K C .................................................................................................................................. Protein_Len: 60 Strand: + Start: 64 End: 246 ............................................................... M K F L V F Q C C F Y C F T E I L A H F S N V K R R S E K L P V C Y L H C R C V L P I K L G D K G K K K P P F P S G K S ............... Protein_Len: 33 Strand: - Start: 134 End: 232 ..................................................................................................................................... F V S R S V V R I S G V N G N H K V S L A P H C P C F S V A R E M ............................. tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1725:PI1726|PI1725:PI1726:hypothetical protein:ATP-dependent DNA helicase:->->:1643281..1643541 261 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Intra-Species Hit: Count: 1 Min: 1 Max: 261 Len: 261 Subject: pint_PI1725_PI1726|hypothetical protein:ATP-dependent DNA helicase|POSITIVE:POSITIVE|[1643281,1643541]|261 HSP 1 e-value: 1.0E-146 bit: 517.0 Len: 261 Query Start:1 Query End:261 Subject Strand: POSITIVE Subject Start: 1 Subject End: 261 tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta tagctttcaccgcgcaaaacctattgttttacctttcaaaaccgctgcttttgaaaaacggaaataaaatttttggtttttcaatgctgtttttattgtttcacggaaatattagctcatttttcaaatgttaaaagacggagcgagaaactacccgtatgctacctacattgccgttgtgttttaccgataaagctgggtgacaagggcaaaaagaaaccgccctttccaagtgggaagagcggttgaaagttcctgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUUUCACCGCGCAAAACCUAUUGUUUUACCUUUCAAAACCGCUGCUUUUGAAAAACGGAAAUAAAAUUUUUGGUUUUUCAAUGCUGUUUUUAUUGUUUCACGGAAAUAUUAGCUCAUUUUUCAAAUGUUAAAAGACGGAGCGAGAAACUACCCGUAUGCUACCUACAUUGCCGUUGUGUUUUACCGAUAAAGCUGGGUGACAAGGGCAAAAAGAAACCGCCCUUUCCAAGUGGGAAGAGCGGUUGAAAGUUCCUGUUA .(((((((.....(((.....................(((((.((.....(((((((((.((((......)))).))))))))).)).))))).........((((........((((.(((((........)))))...))))..........)))).)))........(((((.((((....((((.(......).)))))))).))))).....((((((.((((((....)))))).)))))))))))))....... (-64.07) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAGGAACUUUCAACCGCUCUUCCCACUUGGAAAGGGCGGUUUCUUUUUGCCCUUGUCACCCAGCUUUAUCGGUAAAACACAACGGCAAUGUAGGUAGCAUACGGGUAGUUUCUCGCUCCGUCUUUUAACAUUUGAAAAAUGAGCUAAUAUUUCCGUGAAACAAUAAAAACAGCAUUGAAAAACCAAAAAUUUUAUUUCCGUUUUUCAAAAGCAGCGGUUUUGAAAGGUAAAACAAUAGGUUUUGCGCGGUGAAAGCUA ..(((((..(....((((((((((.((....)).)))))))))).......)..))))).....(((((((((((((((((...((((.(((((...((((..((((((........)).)))).......((((.....)))).))))))))).))))...((....(((((.((.(((((((((..(((......)))..))))))))).....)).))))).....))..........))))))).))))).))))). (-63.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 450 50 3 75 26 pint:PI1829|5end_hypothetical_protein_1769255..1769485_POSITIVE 413 53 11 127 84 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 397 53 12 78 37 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 393 248 201 58 7 pint:PI1070|5end_peptidoglycan-associated_lipoprotein_986110..986340_POSITIVE 392 60 12 83 34 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 392 60 12 55 6 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 392 60 12 55 6 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 391 239 193 113 58 pint:PI2166|5end_hypothetical_protein_2111394..2111624_POSITIVE 379 53 11 66 23 pint:PI2144|5end_hypothetical_protein_2091954..2092184_POSITIVE 378 53 11 78 35 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 374 210 163 140 97 pint:PI0931|5end_hypothetical_protein_862652..862882_POSITIVE 369 53 12 105 61 pint:PI1604|5end_hypothetical_protein_1534017..1534247_POSITIVE 369 53 12 78 34 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 369 53 12 66 22 pint:PI1129|5end_hypothetical_protein_1055377..1055607_POSITIVE 369 53 12 204 160 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 369 50 3 206 157 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 361 49 4 220 168 pint:PI0695|5end_xanthine/uracil_permease_family_protein_631413..631643_POSITIVE 360 50 3 75 26 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 358 60 12 66 17 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 358 60 12 225 176 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 436 60 11 125 77 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 413 53 11 61 18 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 369 53 12 61 17 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 347 53 11 132 87 pint:PI1005|3end_hypothetical_protein_926481..926631_POSITIVE 334 60 12 125 76 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 333 248 206 88 48 pint:PI1440|3end_conserved_hypothetical_protein_1363009..1363159_POSITIVE 333 49 3 126 79 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 332 210 161 81 27 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 322 60 11 136 87 pint:PI0885|3end_hypothetical_protein_817563..817713_POSITIVE 319 247 207 150 113 pint:PI0936|3end_conserved_hypothetical_protein_869464..869614_POSITIVE 319 245 201 45 6 pint:PI0267|3end_conjugative_transposon_protein,_TraQ_259291..259441_POSITIVE 317 60 12 136 88 pint:PI1797|3end_hypothetical_protein_1731559..1731709_POSITIVE 317 60 12 136 88 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 317 60 12 136 88 pint:PI0820|3end_hypothetical_protein_759980..760130_POSITIVE 317 60 12 75 27 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 317 179 139 148 105 pint:PI0651|3end_HD_superfamily_hydrolase_592895..593045_POSITIVE 315 179 134 66 24 pint:PI1827|3end_peptidase,_M23/M37_family,_N-terminal_1768602..1768752_POSITIVE 309 49 7 126 83 pint:PI0118|3end_hypothetical_protein_111640..111790_POSITIVE 306 60 14 136 93 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 305 180 137 107 55 pint:PI1788|3end_hypothetical_protein_1718707..1718857_POSITIVE