Origin IGS: tgatgcgttgttacaaggttttcacttgtcatcacctcgtcgatggtcttgtcaaagcggcgttcgaagcgcaagtcgcggtttgtaacaatacctaccaagtgattgtcttcatcaacaacaggaataccaccgatatggtactctgccatcattgctaaagcctctcttacggtttttcctctacgaatggtaacagggtcgtaaatcataccattctcggcacgcttcacgatagcaacctgtcgtgcttgctcctcgatggtcatattcttgtgtatcacaccgattccaccctcacgtgctatggcaatagccatcgaactttccgttacggtgtccatcgccgctgtaacgaaaggaatgttcagttctatgttacgtgagaacttggttttcaactccacccctttgggtagtatctctgaataagctggtattaaaaggacgtcatcgtaagtcaatccgtccattacgattttatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacctttatcgctgattaaaaacaccttatctattgcacgtgaaaaactcgaatattcgtaaacaaaaaactttttgctcaaaaaacgcgattctcagcgatgttccacgccttacttttaacaatccaagcagcgtgtttaatcttttttaagaagctacatctatcatcgttatgagatagaaaagtggcaaataatgtatctaacttataatacaatattattaattttgcatgattaccatttcgatgtaaaaggaaagaaataaagaaaaaagagttttagcagttgaggcaagttaccctatgcatttgtcgtttcgctgatgcaccgaaacaagtgattataagataagcagaaaaatgcgttaaatacttttaatacaaggtagaaaagtgggaaaatacctgctgaaatgaattataattagtaaatttgcacgcaataaattttctaatatacactatgtcttcatttgttgcagataaaatcgtaatggacggattgacttacgatgacgtccttttaataccagcttattcagagatactacccaaaggggtggagttgaaaaccaagttctcacgtaacatagaactgaacattcctttcgttacagcggcgatggacaccgtaacggaaagttcgatggctattgccatagcacgtgagggtggaatcggtgtgatacacaagaatatgaccatcgaggagcaagcacgacaggttgctatcgtgaagcgtgccgagaatggtatgatttacgaccctgttaccattcgtagaggaaaaaccgtaagagaggctttagcaatgatggcagagtaccatatcggtggtattcctgttgttgatgaagacaatcacttggtaggtattgttacaaaccgcgacttgcgcttcgaacgccgctttgacaagaccatcgacgaggtgatgacaagtgaaaaccttgtaacaacgcatca Mask Tandem Repeat Region ================================================ tgatgcgttgttacaaggttttcacttgtcatcacctcgtcgatggtcttgtcaaagcggcgttcgaagcgcaagtcgcggtttgtaacaatacctaccaagtgattgtcttcatcaacaacaggaataccaccgatatggtactctgccatcattgctaaagcctctcttacggtttttcctctacgaatggtaacagggtcgtaaatcataccattctcggcacgcttcacgatagcaacctgtcgtgcttgctcctcgatggtcatattcttgtgtatcacaccgattccaccctcacgtgctatggcaatagccatcgaactttccgttacggtgtccatcgccgctgtaacgaaaggaatgttcagttctatgttacgtgagaacttggttttcaactccacccctttgggtagtatctctgaataagctggtattaaaaggacgtcatcgtaagtcaatccgtccattacgattttatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Find is-nt database================================================ Query_seq: PI1723:PI1725|PI1723:PI1725:conserved hypothetical protein:hypothetical protein:->->:1642194..1643163 970 tgatgcgttgttacaaggttttcacttgtcatcacctcgtcgatggtcttgtcaaagcggcgttcgaagcgcaagtcgcggtttgtaacaatacctaccaagtgattgtcttcatcaacaacaggaataccaccgatatggtactctgccatcattgctaaagcctctcttacggtttttcctctacgaatggtaacagggtcgtaaatcataccattctcggcacgcttcacgatagcaacctgtcgtgcttgctcctcgatggtcatattcttgtgtatcacaccgattccaccctcacgtgctatggcaatagccatcgaactttccgttacggtgtccatcgccgctgtaacgaaaggaatgttcagttctatgttacgtgagaacttggttttcaactccacccctttgggtagtatctctgaataagctggtattaaaaggacgtcatcgtaagtcaatccgtccattacgattttatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1723:PI1725|PI1723:PI1725:conserved hypothetical protein:hypothetical protein:->->:1642194..1643163 970 tgatgcgttgttacaaggttttcacttgtcatcacctcgtcgatggtcttgtcaaagcggcgttcgaagcgcaagtcgcggtttgtaacaatacctaccaagtgattgtcttcatcaacaacaggaataccaccgatatggtactctgccatcattgctaaagcctctcttacggtttttcctctacgaatggtaacagggtcgtaaatcataccattctcggcacgcttcacgatagcaacctgtcgtgcttgctcctcgatggtcatattcttgtgtatcacaccgattccaccctcacgtgctatggcaatagccatcgaactttccgttacggtgtccatcgccgctgtaacgaaaggaatgttcagttctatgttacgtgagaacttggttttcaactccacccctttgggtagtatctctgaataagctggtattaaaaggacgtcatcgtaagtcaatccgtccattacgattttatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 9 Max: 482 Len: 474 Subject: gi|89109314|ref|AP_003094.1| IMP dehydrogenase [Escherichia coli W3110] HSP 1 e-value: 1.0E-37 bit: 153.0 Len: 474 Query Start:9 Query End:482 Subject Strand: null Subject Start: 3 Subject End: 161 ........ K I V M D G L T Y D D V L L I P A Y S E I L P K G V E L K T K F S R N I E L N I P F V T A A M D T V T E S S M A I A I A R E G G I G V I H K N M T I E E Q A R Q V A I V K R A E N G M I Y D P V T I R R G K T V R E A L A M M A E Y H I G G I P V V D E D N H L V G I V T N R D L R F E R R F D K T I D E V M T - S E N L V T ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........ R I A K E A L T F D D V L L V P A H S T V L P N T A D L S T Q L T K T I R L N I P M L S A A M D T V T E A R L A I A L A Q E G G I G F I H K N M S I E R Q A E E V R R V K K H E S G V V T D P Q T V L P T T T L R E V K E L T E R N G F A G Y P V V T E E N E L V G I I T G R D V R F V T D L N Q P V S V Y M T P K E R L V T ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find nr database================================================ Query_seq: PI1723:PI1725|PI1723:PI1725:conserved hypothetical protein:hypothetical protein:->->:1642194..1643163 970 tgatgcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatgcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 3 End: 158 .. M R C Y K V F T C H H L V D G L V K A A F E A Q V A V C N N T Y Q V I V F I N N R N T T D M V L C H H C ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 33 Strand: + Start: 106 End: 204 ......................................................................................................... M S S S T T G I P P I W Y S A I I A K A S L T V F P L R M V T G S .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 56 Strand: + Start: 216 End: 383 ....................................................................................................................................................................................................................... M L G T L H D S N L S C L L L D G H I L V Y H T D S T L T C Y G N S H R T F R Y G V H R R C N E R N V Q F Y V T ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 208 End: 429 ............................................................................................................................................................................................................... M I P F S A R F T I A T C R A C S S M V I F L C I T P I P P S R A M A I A I E L S V T V S I A A V T K G M F S S M L R E ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 40 Strand: + Start: 383 End: 502 .............................................................................................................................................................................................................................................................................................................................................................................................. M R T W F S T P P L W V V S L N K L V L K G R H R K S I R P L R F Y L Q Q M K T .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 472 End: 588 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T I L S A T N E D I V Y I R K F I A C K F T N Y N S F Q Q V F S H F S T L Y .............................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 509 End: 598 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L E N L L R A N L L I I I H F S R Y F P T F L P C I K S I .................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 552 End: 662 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S A G I F P L F Y L V L K V F N A F F C L S Y N H L F R C I S E T T N A .................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 703 End: 888 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M S F L L H R N G N H A K L I I L Y Y K L D T L F A T F L S H N D D R C S F L K K I K H A A W I V K S K A W N I A E N R .................................................................................. Protein_Len: 35 Strand: + Start: 836 End: 940 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L G L L K V R R G T S L R I A F F E Q K V F C L R I F E F F T C N R .............................. Protein_Len: 39 Strand: - Start: 820 End: 936 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F L N F V S S P N N F T L R P V D S L I A N K S C F T K Q K R I N S N K V H M .................................. Protein_Len: 41 Strand: - Start: 795 End: 917 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L S S L H L K K F F I L C A A Q I T L L L A H F M A S F R T K Q A F L K K N V F M ..................................................... Protein_Len: 35 Strand: - Start: 704 End: 808 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... K K G K V D F H Y D H L I L L I T N Y T L Y M I Q W K E I E Y R H Y M .................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 533 .. V F P I N L E I N R S F K T K L E V G K P L I E S Y A P I L L V D D Y T L G D M V I K D A V F S S M T Y I L F N I A H M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................... tgatgcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 88 Start: 597 End: 626 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTAACGCATTTTTCTGCTTATCTTATAAT........................................................................................................................................................................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatgcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1723:PI1725|PI1723:PI1725:conserved hypothetical protein:hypothetical protein:->->:1642194..1643163 970 tgatgcgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaactcttttttctttatttctttccttttacatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca Intra-Species Hit: Count: 1 Min: 483 Max: 970 Len: 488 Subject: pint_PI1723_PI1725|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1642194,1643163]|970 HSP 1 e-value: 1.0E-141 bit: 504.0 Len: 254 Query Start:717 Query End:970 Subject Strand: POSITIVE Subject Start: 717 Subject End: 970 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................acatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................acatcgaaatggtaatcatgcaaaattaataatattgtattataagttagatacattatttgccacttttctatctcataacgatgatagatgtagcttcttaaaaaagattaaacacgctgcttggattgttaaaagtaaggcgtggaacatcgctgagaatcgcgttttttgagcaaaaagttttttgtttacgaatattcgagtttttcacgtgcaatagataaggtgtttttaatcagcgataaaggtca HSP 2 e-value: 1.0E-114 bit: 414.0 Len: 209 Query Start:483 Query End:691 Subject Strand: POSITIVE Subject Start: 483 Subject End: 691 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaact....................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atctgcaacaaatgaagacatagtgtatattagaaaatttattgcgtgcaaatttactaattataattcatttcagcaggtattttcccacttttctaccttgtattaaaagtatttaacgcatttttctgcttatcttataatcacttgtttcggtgcatcagcgaaacgacaaatgcatagggtaacttgcctcaactgctaaaact....................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCUGCAACAAAUGAAGACAUAGUGUAUAUUAGAAAAUUUAUUGCGUGCAAAUUUACUAAUUAUAAUUCAUUUCAGCAGGUAUUUUCCCACUUUUCUACCUUGUAUUAAAAGUAUUUAACGCAUUUUUCUGCUUAUCUUAUAAUCACUUGUUUCGGUGCAUCAGCGAAACGACAAAUGCAUAGGGUAACUUGCCUCAACUGCUAAAACUCUUUUUUCUUUAUUUCUUUCCUUUUACAUCGAAAUGGUAAUCAUGCAAAAUUAAUAAUAUUGUAUUAUAAGUUAGAUACAUUAUUUGCCACUUUUCUAUCUCAUAACGAUGAUAGAUGUAGCUUCUUAAAAAAGAUUAAACACGCUGCUUGGAUUGUUAAAAGUAAGGCGUGGAACAUCGCUGAGAAUCGCGUUUUUUGAGCAAAAAGUUUUUUGUUUACGAAUAUUCGAGUUUUUCACGUGCAAUAGAUAAGGUGUUUUUAAUCAGCGAUAAAGGUCA ................(((....(((..((((((((.((((((...(((....(((((....((((((((...((((.((((..(((((..(((..(((...)))..))).((((((...(((......)))...............((((((((.((...)).)))))))).))))))...))).))..))))..............((((((((..(((((((..............)))))))..................((((.((((((........)))))).)))).((.((...((((((((.....)).)))))).)).)).....))))))))........))))..))))))))....))))).((((((((....(((..((((..(((.....((((((((....)))))))))))...)))).))).)))))))))))...))))))...))))))))..)))......))). (-92.74) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACCUUUAUCGCUGAUUAAAAACACCUUAUCUAUUGCACGUGAAAAACUCGAAUAUUCGUAAACAAAAAACUUUUUGCUCAAAAAACGCGAUUCUCAGCGAUGUUCCACGCCUUACUUUUAACAAUCCAAGCAGCGUGUUUAAUCUUUUUUAAGAAGCUACAUCUAUCAUCGUUAUGAGAUAGAAAAGUGGCAAAUAAUGUAUCUAACUUAUAAUACAAUAUUAUUAAUUUUGCAUGAUUACCAUUUCGAUGUAAAAGGAAAGAAAUAAAGAAAAAAGAGUUUUAGCAGUUGAGGCAAGUUACCCUAUGCAUUUGUCGUUUCGCUGAUGCACCGAAACAAGUGAUUAUAAGAUAAGCAGAAAAAUGCGUUAAAUACUUUUAAUACAAGGUAGAAAAGUGGGAAAAUACCUGCUGAAAUGAAUUAUAAUUAGUAAAUUUGCACGCAAUAAAUUUUCUAAUAUACACUAUGUCUUCAUUUGUUGCAGAU .......(((((((((.................(((((............(((....)))....(((((....)))))..........)))))..)))))))))((((((...(((((((.......((...((((.((((...((((((((.....(((((.((((((.((.....))))))))...))))).((((.(((((..........))))).)))).((..((((((((((........)).))))))))..))..........)))))))).....)))))))).)).((((......((((((((.((((((..((....(((........)))......))..)))).)).)))))))).....)))).......)))))))....))))))......((((..(((((((.(((((((((.(((((((.......))))))).)))))......))))..)))))))...)))).. (-88.71) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 199 152 49 3 pint:PI1701|5end_sensor_histidine_kinase_1619312..1619542_POSITIVE 334 394 351 85 35 pint:PI0030|5end_transcriptional_regulator,_AsnC_family_34509..34739_POSITIVE 330 401 363 62 19 pint:PI1894|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino_pimelate_ligase_1836947..1837177_POSITIVE 325 246 208 226 182 pint:PI0328|5end_conserved_hypothetical_protein_309476..309706_POSITIVE 311 166 121 59 15 pint:PI0692|5end_iron_compound_ABC_transporter,_permease_protein_627350..627580_POSITIVE 310 399 353 60 14 pint:PI1476|5end_acyl-CoA_synthase/O-succinylbenzoic_acid-CoA_ligase_1397699..1397929_POSITIVE 304 398 351 176 133 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 300 207 162 141 80 pint:PI1468|5end_conserved_hypothetical_protein;_probable_TPR_domain_protein_1393900..1394130_POSITIVE 299 397 351 185 149 pint:PI0770|5end_hypothetical_protein_706170..706400_POSITIVE 299 400 352 211 165 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 298 390 341 99 57 pint:PI1586|5end_possible_HesA/MoeB/ThiF_family_protein_1514372..1514602_POSITIVE 296 400 352 71 25 pint:PI1697|5end_conserved_hypothetical_protein_1612217..1612447_POSITIVE 294 417 371 85 33 pint:PI1150|5end_30S_ribosomal_protein_S6_1075683..1075913_POSITIVE 293 193 155 143 104 pint:PI1893|5end_phospho-N-acetylmuramoyl-pentapeptide-transferase__1835624..1835854_POSITIVE 290 229 183 185 138 pint:PI2086|5end_signal_peptidase_I_2041835..2042065_POSITIVE 289 188 147 180 134 pint:PI0032|5end_hypothetical_protein_36085..36315_POSITIVE 285 418 371 189 140 pint:PI0269|5end_conserved_hypothetical_protein_259835..260065_POSITIVE 284 195 153 147 95 pint:PI1522|5end_thioredoxin_M_1441646..1441876_POSITIVE 281 165 128 33 1 pint:PI1999|5end_hypothetical_protein_1956457..1956687_POSITIVE 279 411 372 116 68 pint:PI1914|5end_RNA_polymerase,_ECF-type_sigma_factor_1858025..1858255_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 320 400 351 141 90 pint:PI1788|3end_hypothetical_protein_1718707..1718857_POSITIVE 300 207 162 70 9 pint:PI1467|3end_conserved_hypothetical_protein_1393909..1394059_POSITIVE 300 412 372 151 104 pint:PI1366|3end_conserved_hypothetical_protein;_possible_hydrolase,_HAD_superfamily_1286647..1286797_POSITIVE 299 397 351 107 71 pint:PI0769|3end_phosphomannomutase_706172..706322_POSITIVE 294 417 371 79 27 pint:PI1149|3end_30S_ribosomal_protein_S18_1075757..1075907_POSITIVE 290 229 183 73 26 pint:PI2085|3end_conserved_hypothetical_protein_2041803..2041953_POSITIVE 289 188 147 146 100 pint:PI0031|3end_peptidylprolyl_isomerase_36131..36281_POSITIVE 285 418 371 64 15 pint:PI0268|3end_lysozyme-related_protein_259790..259940_POSITIVE 279 398 352 65 10 pint:PI0629|3end_heat_shock_protein_15_577122..577272_POSITIVE 279 199 157 126 92 pint:PI0036|3end_glutathione_peroxidase_39289..39439_POSITIVE 276 412 379 144 107 pint:PI1274|3end_possible_phosphoribosylanthranilate_isomerase_1193604..1193754_POSITIVE 274 399 352 60 19 pint:PI1616|3end_hypothetical_protein_1542669..1542819_POSITIVE 273 217 172 136 90 pint:PI0093|3end_riboflavin_biosynthesis_protein_91537..91687_POSITIVE 272 187 147 115 74 pint:PI1723|3end_conserved_hypothetical_protein_1642133..1642283_POSITIVE 269 160 112 75 30 pint:PI0351|3end_hypothetical_protein_324768..324918_POSITIVE 269 160 112 142 97 pint:PI0350|3end_conserved_hypothetical_protein_324835..324985_POSITIVE 269 413 376 91 48 pint:PI0114|3end_hypothetical_protein_108527..108677_POSITIVE 268 194 153 144 102 pint:PI0428|3end_hypothetical_protein_396321..396471_POSITIVE 266 193 158 72 32 pint:PI1379|3end_hypothetical_protein_1302292..1302442_POSITIVE 265 230 183 111 62 pint:PI1223|3end_conserved_hypothetical_protein_1144459..1144609_POSITIVE