Origin IGS: cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataaaacaaacttcgctctcgttataatgagaaaagaatatcgtttcactataaataataagtttaaaacaagcctcaatacaagcgaggcatagaatgaatatcgtttgaaatgagaataatagcatttttcgctgccgcactgcttagcagcacg Mask Tandem Repeat Region ================================================ cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Find is-nt database================================================ Query_seq: PI1722:PI1724|PI1722:PI1724:possible peptidyl-prolyl cis-trans isomerase:inosine-5'-monophosphate dehydrogenase:->->:1641056..1641214 159 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1722:PI1724|PI1722:PI1724:possible peptidyl-prolyl cis-trans isomerase:inosine-5'-monophosphate dehydrogenase:->->:1641056..1641214 159 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1722:PI1724|PI1722:PI1724:possible peptidyl-prolyl cis-trans isomerase:inosine-5'-monophosphate dehydrogenase:->->:1641056..1641214 159 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 15 End: 158 .............. M R Q R K M L L F S F Q T I F I L C L A C I E A C F K L I I Y S E T I F F S H Y N E S E V C F I . cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1722:PI1724|PI1722:PI1724:possible peptidyl-prolyl cis-trans isomerase:inosine-5'-monophosphate dehydrogenase:->->:1641056..1641214 159 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Intra-Species Hit: Count: 1 Min: 1 Max: 159 Len: 159 Subject: pint_PI1722_PI1724|possible peptidyl-prolyl cis-trans isomerase:inosine-5'-monophosphate dehydrogenase|POSITIVE:POSITIVE|[1641056,1641214]|159 HSP 1 e-value: 2.0E-85 bit: 315.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 1 Subject End: 159 cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta cgtgctgctaagcagtgcggcagcgaaaaatgctattattctcatttcaaacgatattcattctatgcctcgcttgtattgaggcttgttttaaacttattatttatagtgaaacgatattcttttctcattataacgagagcgaagtttgttttatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUGCUGCUAAGCAGUGCGGCAGCGAAAAAUGCUAUUAUUCUCAUUUCAAACGAUAUUCAUUCUAUGCCUCGCUUGUAUUGAGGCUUGUUUUAAACUUAUUAUUUAUAGUGAAACGAUAUUCUUUUCUCAUUAUAACGAGAGCGAAGUUUGUUUUAUUA ((..((((...))))..))..((((.....))))..................((((..((..((.(((((((.(((((.(((((.((((((((....((.....))...))))))))........))))).))))))))).))).))..))...)))). (-35.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAAACAAACUUCGCUCUCGUUAUAAUGAGAAAAGAAUAUCGUUUCACUAUAAAUAAUAAGUUUAAAACAAGCCUCAAUACAAGCGAGGCAUAGAAUGAAUAUCGUUUGAAAUGAGAAUAAUAGCAUUUUUCGCUGCCGCACUGCUUAGCAGCACG .............(((..((((((....))))))...)))..(((((((.........................(((((.........)))))...(((((.....)))))))))))).................(((((.((...))...)))))... (-30.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 89 56 195 149 pint:PI0010|5end_conserved_hypothetical_protein_10628..10858_POSITIVE 294 26 1 191 165 pint:PI2112|5end_phosphopantetheine_adenylyltransferase_2063194..2063424_POSITIVE 292 26 1 174 139 pint:PI1285|5end_ATP_synthase,_B_subunit_1200348..1200578_POSITIVE 289 88 52 38 1 pint:PI1382|5end_hypothetical_protein_1303608..1303838_POSITIVE 284 60 11 181 128 pint:PI0817|5end_glycosyl_transferase,_group_1_family_protein_756861..757091_POSITIVE 281 42 2 56 13 pint:PI1913|5end_conserved_hypothetical_protein_1857104..1857334_POSITIVE 278 44 1 191 149 pint:PI0442|5end_50S_ribosomal_protein_L1_411644..411874_POSITIVE 276 43 1 183 130 pint:PI1768|5end_conserved_hypothetical_protein_1695456..1695686_POSITIVE 274 37 1 77 43 pint:PI0294|5end_conserved_hypothetical_protein_276223..276453_POSITIVE 273 53 11 204 164 pint:PI1603|5end_hypothetical_protein_1533779..1534009_POSITIVE 272 35 1 132 95 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 271 50 2 212 156 pint:PI0468|5end_50S_ribosomal_protein_L29_436905..437135_POSITIVE 270 46 1 92 37 pint:PI0258|5end_conjugative_transposon_protein,_TraG_249722..249952_POSITIVE 268 33 1 173 140 pint:PI2109|5end_surface_antigen_BspA_2060553..2060783_POSITIVE 268 26 1 26 1 pint:PI1892|5end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_1834217..1834447_POSITIVE 268 25 1 222 200 pint:PI0596|5end_hypothetical_protein_546400..546630_POSITIVE 267 89 48 106 64 pint:PI1910|5end_hypothetical_protein_1855778..1856008_POSITIVE 263 34 3 51 18 pint:PI1985|5end_hypothetical_protein_1940291..1940521_POSITIVE 263 49 1 111 55 pint:PI0207|5end_conserved_hypothetical_protein_199797..200027_POSITIVE 263 48 7 226 186 pint:PI0077|5end_hypothetical_protein_76244..76474_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 37 1 80 50 pint:PI0779|3end_predicted_TPR-repeat-containing_protein_715800..715950_POSITIVE 292 26 1 69 34 pint:PI1284|3end_ATP_synthase,_delta_subunit_1200323..1200473_POSITIVE 286 34 2 50 22 pint:PI0324|3end_conserved_hypothetical_protein_305680..305830_POSITIVE 283 43 1 104 57 pint:PI0297|3end_hypothetical_protein_277916..278066_POSITIVE 270 46 1 92 37 pint:PI0257|3end_conjugative_transposon_protein,_TraF_249802..249952_POSITIVE 268 25 1 148 126 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 267 85 49 151 116 pint:PI1448|3end_hypothetical_protein_1371832..1371982_POSITIVE 267 60 11 143 107 pint:PI0816|3end_glycogen_debranching_enzyme_756920..757070_POSITIVE 263 48 2 99 56 pint:PI0798|3end_malonyl_CoA-acyl_carrier_protein_transacylase_736975..737125_POSITIVE 263 49 1 90 34 pint:PI0206|3end_TPR-repeat-containing_protein_199856..200006_POSITIVE 263 48 7 94 54 pint:PI0076|3end_conserved_hypothetical_protein_76192..76342_POSITIVE 262 49 2 74 18 pint:PI0526|3end_hypothetical_protein_482439..482589_POSITIVE 262 33 3 116 89 pint:PI0036|3end_glutathione_peroxidase_39289..39439_POSITIVE 261 48 3 143 96 pint:PI2132|3end_hypothetical_protein_2081139..2081289_POSITIVE 261 25 1 68 49 pint:PI0111|3end_hypothetical_protein_108265..108415_POSITIVE 259 43 1 62 16 pint:PI1831|3end_conserved_hypothetical_protein_1773167..1773317_POSITIVE 258 88 49 71 28 pint:PI0792|3end_conserved_hypothetical_protein_729627..729777_POSITIVE 257 25 1 141 109 pint:PI0578|3end_methyltransferase_523253..523403_POSITIVE 256 48 1 94 37 pint:PI1667|3end_conserved_hypothetical_protein_1580772..1580922_POSITIVE 254 48 5 74 35 pint:PI0632|3end_NusB_family_protein_(N_utilization_substance_protein_B)_579731..579881_POSITIVE