Origin IGS: taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacgcttatataaacgactgaaaaccgattttattgtatgccgttatgccctctttggcacgtatggaaacaataagggcgcacctgaaattcaggtacgcccttgttatgtaagtttaatgataaccgaatgagaagtaactcatta Mask Tandem Repeat Region ================================================ taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Find is-nt database================================================ Query_seq: PI1700:PI1701|PI1700:PI1701:hypothetical protein:sensor histidine kinase:->->:1619304..1619451 148 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1700:PI1701|PI1700:PI1701:hypothetical protein:sensor histidine kinase:->->:1619304..1619451 148 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1700:PI1701|PI1700:PI1701:hypothetical protein:sensor histidine kinase:->->:1619304..1619451 148 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 17 End: 115 ................ M R L S L N L H N K G V P E F Q V R P Y C F H T C Q R G H N G I Q ................................. Protein_Len: 34 Strand: + Start: 39 End: 140 ...................................... M T R A Y L N F R C A L I V S I R A K E G I T A Y N K I G F Q S F I ........ Protein_Len: 38 Strand: - Start: 18 End: 131 ................. E T I M L S V Y C P R V Q I E P A G K N N G Y T G F L A Y R C V I F D T K M ................. taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -18.8 Tail_Score: -4.10744 Start: 46 End: 72 Full_Region: AAACTTACATAACAA GGGCGTACCTGA ATT TCAGGTGCGCCC TTATTGTTTCCATAC .............................................GGGCGTACCTGAATTTCAGGTGCGCCC............................................................................ TransTerm Strand: - Conf: 100 HP_score: -16.6 Tail_Score: -3.91364 Start: 46 End: 72 Full_Region: gtatggaaacaataa gggcgcacctga aat tcaggtacgccc ttgttatgtaagttt .............................................gggcgcacctgaaattcaggtacgccc............................................................................ Find igs database================================================ Query_seq: PI1700:PI1701|PI1700:PI1701:hypothetical protein:sensor histidine kinase:->->:1619304..1619451 148 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Intra-Species Hit: Count: 1 Min: 1 Max: 148 Len: 148 Subject: pint_PI1700_PI1701|hypothetical protein:sensor histidine kinase|POSITIVE:POSITIVE|[1619304,1619451]|148 HSP 1 e-value: 8.0E-79 bit: 293.0 Len: 148 Query Start:1 Query End:148 Subject Strand: POSITIVE Subject Start: 1 Subject End: 148 taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt taatgagttacttctcattcggttatcattaaacttacataacaagggcgtacctgaatttcaggtgcgcccttattgtttccatacgtgccaaagagggcataacggcatacaataaaatcggttttcagtcgtttatataagcgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAGUUACUUCUCAUUCGGUUAUCAUUAAACUUACAUAACAAGGGCGUACCUGAAUUUCAGGUGCGCCCUUAUUGUUUCCAUACGUGCCAAAGAGGGCAUAACGGCAUACAAUAAAAUCGGUUUUCAGUCGUUUAUAUAAGCGUU .((((((......))))))((.((((...(((((..........((((((((((((.....))))))))))))((((((..((....(((((......)))))...))...))))))................))))).)))).)).. (-46.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACGCUUAUAUAAACGACUGAAAACCGAUUUUAUUGUAUGCCGUUAUGCCCUCUUUGGCACGUAUGGAAACAAUAAGGGCGCACCUGAAAUUCAGGUACGCCCUUGUUAUGUAAGUUUAAUGAUAACCGAAUGAGAAGUAACUCAUUA ...((((((((((.................(((((((...((((..((((......))))...))))..)))))))(((((.(((((.....))))).)))))...)))))))))).............((((((......)))))). (-45.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 372 110 68 71 20 pint:PI0492|5end_hypothetical_protein_451701..451931_POSITIVE 366 110 63 74 21 pint:PI0408|5end_hypothetical_protein_374154..374384_POSITIVE 366 89 46 174 124 pint:PI0313|5end_conserved_hypothetical_protein_294833..295063_POSITIVE 366 89 46 62 12 pint:PI0311|5end_hypothetical_protein_294721..294951_POSITIVE 353 109 63 153 84 pint:PI1849|5end_conserved_hypothetical_protein;_possible_acyltransferase_family_protein_1794916..1795146_POSITIVE 337 110 65 60 29 pint:PI0265|5end_conjugative_transposon_protein,_TraO_257296..257526_POSITIVE 336 82 41 137 96 pint:PI0826|5end_hypothetical_protein_763738..763968_POSITIVE 336 82 41 117 76 pint:PI0825|5end_hypothetical_protein_763718..763948_POSITIVE 333 109 66 173 137 pint:PI2115|5end_hypothetical_protein_2064966..2065196_POSITIVE 329 108 66 171 116 pint:PI2014|5end_conserved_hypothetical_protein_1971471..1971701_POSITIVE 324 84 44 162 119 pint:PI0142|5end_DNA-binding_protein_133839..134069_POSITIVE 321 109 61 178 134 pint:PI1069|5end_hypothetical_protein_988067..988297_POSITIVE 317 98 52 49 1 pint:PI0576|5end_helicase,_UvrD/Rep_520226..520456_POSITIVE 317 109 62 109 57 pint:PI0304|5end_hypothetical_protein_288582..288812_POSITIVE 316 123 87 207 165 pint:PI1038|5end_hypothetical_protein_958685..958915_POSITIVE 313 96 64 224 191 pint:PI0365|5end_hypothetical_protein_335721..335951_POSITIVE 308 114 81 166 128 pint:PI0280|5end_conserved_hypothetical_protein_266001..266231_POSITIVE 307 109 64 202 153 pint:PI1472|5end_hypothetical_protein_1395671..1395901_POSITIVE 307 109 64 60 11 pint:PI1473|5end_hypothetical_protein_1395529..1395759_POSITIVE 303 109 64 156 119 pint:PI2097|5end_conserved_hypothetical_protein_2050912..2051142_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 537 79 39 52 12 pint:PI1700|3end_hypothetical_protein_1619243..1619393_POSITIVE 390 110 61 79 29 pint:PI1726|3end_ATP-dependent_DNA_helicase_1645542..1645692_POSITIVE 337 110 65 55 24 pint:PI0264|3end_transfer_region-related_protein,_TraN_257371..257521_POSITIVE 336 82 41 59 18 pint:PI0824|3end_large_conductance_mechanosensitive_channel_protein_763740..763890_POSITIVE 312 99 63 149 103 pint:PI1848|3end_hypothetical_protein_1795015..1795165_POSITIVE 307 109 64 87 38 pint:PI1471|3end_conserved_hypothetical_protein_1395636..1395786_POSITIVE 305 110 61 125 66 pint:PI1884|3end_conserved_hypothetical_protein_1827457..1827607_POSITIVE 303 109 64 48 11 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 298 101 64 112 73 pint:PI0579|3end_hypothetical_protein_524209..524359_POSITIVE 291 70 32 150 109 pint:PI0791|3end_conserved_hypothetical_protein_729109..729259_POSITIVE 286 110 61 133 60 pint:PI0889|3end_pyruvate_kinase_820355..820505_POSITIVE 283 108 62 101 65 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 279 83 46 55 9 pint:PI2042|3end_cation-transporting_ATPase_1996138..1996288_POSITIVE 275 77 35 65 19 pint:PI0753|3end_hypothetical_protein_683851..684001_POSITIVE 274 109 63 48 4 pint:PI0766|3end_conserved_hypothetical_protein_701976..702126_POSITIVE 273 109 64 102 53 pint:PI2117|3end_fumarate_reductase/succinate_dehydrogenase_flavoprotein_subunit,_C-terminal_2066684..2066834_POSITIVE 270 80 37 70 25 pint:PI0694|3end_Na+/dicarboxylate_or_sulfate_symporter_631177..631327_POSITIVE 269 96 53 87 29 pint:PI0571|3end_conserved_hypothetical_protein;_possible_permease_516449..516599_POSITIVE 268 110 68 151 120 pint:PI1771|3end_alpha-glucosidase_1702274..1702424_POSITIVE 268 110 61 64 12 pint:PI1121|3end_glycosyltransferase_1046707..1046857_POSITIVE