Origin IGS: tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttttataatgcgttggtagtgagatagttacgcaatagtattgcttgtgaaaaatatttacagttttataaccttattttcagccataaaataagaattgaaggagaagtaacgcagggggcaatttgtataaaataaaggctgactccgtttctgaagtcagcctttatgctttatggtgttcattaaatctacgaggtgctgttactgctatatataaataaggtgtagcta Mask Tandem Repeat Region ================================================ tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Find is-nt database================================================ Query_seq: PI1693:PI1694|PI1693:PI1694:possible RmuC domain protein:hypothetical protein:->->:1609619..1609856 238 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1693:PI1694|PI1693:PI1694:possible RmuC domain protein:hypothetical protein:->->:1609619..1609856 238 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1693:PI1694|PI1693:PI1694:possible RmuC domain protein:hypothetical protein:->->:1609619..1609856 238 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 65 End: 166 ................................................................ M K A D F R N G V S L Y F I Q I A P C V T S P S I L I L W L K I R L ........................................................................ Protein_Len: 32 Strand: - Start: 100 End: 195 ................................................................................................... K I C I A G Q T V E G E I R I K H S F I L N Y F Q L Y K E C A M ........................................... Protein_Len: 32 Strand: - Start: 14 End: 109 ............. K Y I A T V A G R L N L S C W L A Y L S V E S V S D A K I K Y M ................................................................................................................................. tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -14.8 Tail_Score: -4.4017 Start: 66 End: 99 Full_Region: GAACACCATAAAGCA TAAAGGCTGACTTC AGAAAC GGAGTCAGCCTTTA TTTTATACAAATTGC .................................................................TAAAGGCTGACTTCAGAAACGGAGTCAGCCTTTA........................................................................................................................................... TransTerm Strand: - Conf: 88 HP_score: -13.3 Tail_Score: -3.06651 Start: 66 End: 99 Full_Region: gcaatttgtataaaa taaaggctgactccg tttc tgaagtcagccttta tgctttatggtgttc .................................................................taaaggctgactccgtttctgaagtcagccttta........................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -11.2 Tail_Score: -4.77173 Start: 70 End: 95 Full_Region: ACCATAAAGCATAAA GGCTGACTTC AGAAAC GGAGTCAGCC TTTATTTTATACAAA .....................................................................GGCTGACTTCAGAAACGGAGTCAGCC............................................................................................................................................... TransTerm Strand: - Conf: 93 HP_score: -9.7 Tail_Score: -4.18303 Start: 70 End: 95 Full_Region: tttgtataaaataaa ggctgactccg tttc tgaagtcagcc tttatgctttatggt .....................................................................ggctgactccgtttctgaagtcagcc............................................................................................................................................... Find igs database================================================ Query_seq: PI1693:PI1694|PI1693:PI1694:possible RmuC domain protein:hypothetical protein:->->:1609619..1609856 238 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 238 Len: 238 Subject: pint_PI1693_PI1694|possible RmuC domain protein:hypothetical protein|POSITIVE:POSITIVE|[1609619,1609856]|238 HSP 1 e-value: 1.0E-132 bit: 472.0 Len: 238 Query Start:1 Query End:238 Subject Strand: POSITIVE Subject Start: 1 Subject End: 238 tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta tagctacaccttatttatatatagcagtaacagcacctcgtagatttaatgaacaccataaagcataaaggctgacttcagaaacggagtcagcctttattttatacaaattgccccctgcgttacttctccttcaattcttattttatggctgaaaataaggttataaaactgtaaatatttttcacaagcaatactattgcgtaactatctcactaccaacgcattataaaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUACACCUUAUUUAUAUAUAGCAGUAACAGCACCUCGUAGAUUUAAUGAACACCAUAAAGCAUAAAGGCUGACUUCAGAAACGGAGUCAGCCUUUAUUUUAUACAAAUUGCCCCCUGCGUUACUUCUCCUUCAAUUCUUAUUUUAUGGCUGAAAAUAAGGUUAUAAAACUGUAAAUAUUUUUCACAAGCAAUACUAUUGCGUAACUAUCUCACUACCAACGCAUUAUAAAACCUA ..(((......((((((((...((.((((((.(((....((((.....(((.....))).....(((((((((((((((......)))))))))))))))..........))))....))))))))).)).............((((((((((........)))))))))).)))))))).........)))........(((((................)))))............ (-49.25) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUAUAAUGCGUUGGUAGUGAGAUAGUUACGCAAUAGUAUUGCUUGUGAAAAAUAUUUACAGUUUUAUAACCUUAUUUUCAGCCAUAAAAUAAGAAUUGAAGGAGAAGUAACGCAGGGGGCAAUUUGUAUAAAAUAAAGGCUGACUCCGUUUCUGAAGUCAGCCUUUAUGCUUUAUGGUGUUCAUUAAAUCUACGAGGUGCUGUUACUGCUAUAUAUAAAUAAGGUGUAGCUA ....((((((.(((.(((((.((((((........(((((...))))).((((((.....))))))..........)))))).))))))))...))))))...........((((((((...(((((..(..((....(((((((((((((.((....)).)))))))))))))....))..)))))).....(((.....))))).))))))(((((((.........))))))).. (-55.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 426 209 172 181 144 pint:PI1272|5end_possible_transcriptional_regulator_1191702..1191932_POSITIVE 410 218 173 223 178 pint:PI1309|5end_excinuclease_ABC,_A_subunit_1223751..1223981_POSITIVE 399 218 173 115 70 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 384 213 173 180 140 pint:PI0096|5end_glycerol-3-phosphate_cytidylyltransferase_91885..92115_POSITIVE 380 214 171 46 3 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 370 193 151 227 184 pint:PI1206|5end_ATP-dependent_DNA_helicase,_UvrD/PcrA/Rep_family_1124698..1124928_POSITIVE 368 218 173 186 141 pint:PI0090|5end_amidophosphoribosyltransferase_88379..88609_POSITIVE 363 215 173 231 189 pint:PI2030|5end_hypothetical_protein_1985601..1985831_POSITIVE 362 213 173 221 181 pint:PI1253|5end_hypothetical_protein_1175516..1175746_POSITIVE 355 229 181 108 60 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 338 209 166 106 63 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 334 213 173 204 164 pint:PI0660|5end_conserved_hypothetical_protein_603438..603668_POSITIVE 326 208 161 211 163 pint:PI0828|5end_GMP_synthase_(glutamine_amidotransferase)_764077..764307_POSITIVE 326 208 161 125 77 pint:PI0827|5end_hypothetical_protein_763991..764221_POSITIVE 324 213 173 116 76 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 323 208 168 133 94 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 319 215 173 170 128 pint:PI0949|5end_endonuclease_882264..882494_POSITIVE 311 220 172 178 123 pint:PI0333|5end_hypothetical_protein_313478..313708_POSITIVE 300 79 32 88 40 pint:PI0351|5end_hypothetical_protein_324317..324547_POSITIVE 299 208 161 111 63 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 469 229 181 120 72 pint:PI0069|3end_hypothetical_protein_67379..67529_POSITIVE 459 213 173 104 64 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 426 213 171 104 62 pint:PI1204|3end_hypothetical_protein_1124635..1124785_POSITIVE 410 218 173 109 64 pint:PI1308|3end_hypothetical_protein_1223717..1223867_POSITIVE 384 213 173 65 25 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 368 218 173 82 37 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 364 210 161 87 37 pint:PI1072|3end_hypothetical_protein_990503..990653_POSITIVE 362 213 173 65 25 pint:PI1252|3end_hypothetical_protein_1175440..1175590_POSITIVE 344 213 173 77 37 pint:PI0820|3end_hypothetical_protein_759980..760130_POSITIVE 335 212 171 151 110 pint:PI1371|3end_hypothetical_protein_1292546..1292696_POSITIVE 334 213 173 82 42 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 330 217 172 77 36 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 330 218 173 82 37 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 326 208 161 99 51 pint:PI0826|3end_hypothetical_protein_764045..764195_POSITIVE 325 214 173 78 37 pint:PI1005|3end_hypothetical_protein_926481..926631_POSITIVE 323 94 53 117 72 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 320 208 161 60 13 pint:PI0815|3end_hypothetical_protein_754677..754827_POSITIVE 315 215 173 140 98 pint:PI1786|3end_hypothetical_protein_1718380..1718530_POSITIVE 305 152 111 151 101 pint:PI1227|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1148974..1149124_POSITIVE 304 229 181 54 6 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE