Origin IGS: aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgagttaaggaagtcgtcaaagacattctgccttgttgttgtagtcaggttctcgtagagagcatctgttatttctttcagcttgccggcttgtgcgatgtagtcattaagcttatcagctacgcgaccaccatagtctcccttacctgtattctggatcttttcccacatatcgacattggaacgtaacatcttcatttcttcgggagtgagattccacaagttgccgtcccattttctgcctatttgagaactgaaacgatttatttgctcctgactaaaccctttccagtaggcgttgaaactatgatgtgccgagtgatagcctgcctgttcctgtgcaatacgtttgtagttctcgttcgtttcacgctgaagtttctctgcatcgcgtgatatgcgaatagctgtagcacctcgtgccgtcttcatctcgtccgttaggtcctcaattgcctgctccaggagttcgttacgttttgtcaggcgatcaattgattttgccacttcctcctcgttactgttagtaaaccagctgctgggacctttatgactgagcagtccgaaggaaagaacgttaccgatacgtccgacaacagtatccaacaaacctccaataccttttactataatagactcaagtaccttaaataaattctctggcaagtcaaaaatagcattgataagattaccgatagcttccaagatgctatttacaagatcgtctatccagcgcaaagatataagttctgtcagtgagttcagaacaccagtaacaaagcttttgatactattggcaaggtcaagtatcattctgggtatttgtgcaataatgccaaccatacttcctatgccacttgatagaatgcccgacatcgtgccaccaagagaggacaacgcatttcccattgctcctgacacagcactgccaacactctttgctatgccgtctcccatggttggaagaatagaatcaagcgtacctttgagtgcatcaacctgtcctactgactgctgtacgcctccgtatccatctacaccctgccatgccttagcgttattgagagccgttgttaatccagacgtaaagttagcaacttcttcagatgtcctatttaatgctttgccgaaggagtccatatcctcgcgtgcttgtactgttgccctccccagctcttcagcacgtgcttcaagttcatcgtatctttccttactgattctcccttcacggagtcctacctttcctgcttccatggcagcaacggcagcagcttcatctttcttaacttggtcgtatacagccacgctatcagcaaatcgcttgatggcttcgtccaacttctgccatgtcgcactctggtcagtaccgatatactgtctcatctgctgaattagctccgtcactttctgttgtgtatcggctgaagcgttcctatagtcgtcagtttctgtgtaggcacgcagctgctccatcatcggttgcatcatctccttggtcagattgcctacaccgctgaagagcgcattccagtcgataccacggctgatttcctcgaacgacatacttgcctggcgttcttgatattccttctgaagctttgctttctgccacttctttgttgtctcgctcacttccgaagcatctatatccgctatctgttgggcataatcttcagcaatcgcaagcttttgctgctgaaaagagccataagtcttgagatattcactcatagcctgcacttcatttctctgctgctctaagcgtttcttttgctcttctttatttatctcatcttcgtt Mask Tandem Repeat Region ================================================ aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Find is-nt database================================================ Query_seq: PI1654:PI1655|PI1654:PI1655:hypothetical protein:hypothetical protein:->->:1568019..1569818 1800 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1654:PI1655|PI1654:PI1655:hypothetical protein:hypothetical protein:->->:1568019..1569818 1800 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1654:PI1655|PI1654:PI1655:hypothetical protein:hypothetical protein:->->:1568019..1569818 1800 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Predict ORF larger than 30AA ================================================ Protein_Len: 56 Strand: + Start: 189 End: 356 ............................................................................................................................................................................................ M A E S K A S E G I S R T P G K Y V V R G N Q P W Y R L E C A L Q R C R Q S D Q G D D A T D D G A A A C L H R N .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: + Start: 428 End: 562 ........................................................................................................................................................................................................................................................................................................................................................................................................................................... M S V L T R V R H G R S W T K P S S D L L I A W L Y T T K L R K M K L L P L L P W K Q E R ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 572 End: 682 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K G E S V R K D T M N L K H V L K S W G G Q Q Y K H A R I W T P S A K H .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 44 Strand: + Start: 1184 End: 1315 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M E V C W I L L S D V S V T F F P S D C S V I K V P A A G L L T V T R R K W Q N Q L I A ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 1385 End: 1534 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L Q L F A Y H A M Q R N F S V K R T R T T N V L H R N R Q A I T R H I I V S T P T G K G L V R S K .......................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 1566 End: 1655 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M G R Q L V E S H S R R N E D V T F Q C R Y V G K D P E Y R ................................................................................................................................................. Protein_Len: 60 Strand: + Start: 13 End: 1800 ............ M N K E E Q K K R L E Q Q R N E V Q A M S E Y L K T Y G S F Q Q Q K L A I A E D Y A Q Q I A D I D A S E V S E T T K K W Protein_Len: 54 Strand: - Start: 1615 End: 1776 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. T G I D I H P F L D L I C T L S V I T T A Y S I L K I V V D C L G A L Q F F Y C I S E V L V Q S C C C P L M ........................ Protein_Len: 30 Strand: - Start: 1554 End: 1643 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I P L F H S P L K H F R V G S S I F I N R E L T S I H S F M ............................................................................................................................................................. Protein_Len: 60 Strand: - Start: 1408 End: 1587 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A I C L F K L T F R V L V V F T N C L F L C A I V R C M M T E V G V P F P K T L L L Y I T E T R L Y A S F P V A V Q P M ..................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 1397 End: 1489 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E C I V R H L S V E A H F S R S S C V Y Q V P V P L S D S P M ....................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 1277 End: 1381 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ C Y R P P L P L I L Q D G S L V Y R V G P A P L Q P G L P R S S S P M ................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 1251 End: 1403 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L P G L L Q N V L L L S S S T A F D I S R R V F R L S S R S C A I S S R V S S I F V A R P A V A I R M ............................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 34 Strand: - Start: 1150 End: 1251 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K L Y K L R N Y Y F T N S T Q Q I S N D S T D T V N K G E S Q E T M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 1045 End: 1146 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q C F K Y R Q A P Y V I K Y I A D Q F S D T I K D I S N K V Q W L M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 37 Strand: - Start: 833 End: 943 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ E E L W P L R C L L S H Q C H Q T L L L P F H T T R E Q H C S T P C E I M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: - Start: 587 End: 694 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Y P F I R H V Q L V H Q L A P S P L L V L V R P Y P S R R C L M L Y S M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 414 End: 1247 ............................................................................................................................................................................................................................................................................................................................................................................................................................. Q I S S R T F L M K S A I P L R I L A I K S K G S F K N L T S S D I I T F P I P P K N S V T T P R I P L T R E K P S S M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 409 End: 660 ........................................................................................................................................................................................................................................................................................................................................................................................................................ S K S I A H S Y V V L N L F I F S S G N S G H F C S L Y S E T F P S D T L F S V I F K F C T S F L Q P P C C Y L C A L M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 56 Strand: - Start: 287 End: 454 .............................................................................................................................................................................................................................................................................................. R H L C D S W P S S A V S S P A A A H R C L F Q R S Y S R K L R Y V V S L S P A L E A S S V T Y R Y Q G S H S M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 36 Strand: - Start: 222 End: 329 ............................................................................................................................................................................................................................. S R W A L I D N S S I L R P I S Q F A S K L P T P L R V L S I I C G I M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: - Start: 86 End: 235 ..................................................................................... S K H S K E A A F A Q S Q Q L N H G V S L P Y L H K P L S R S L L S T A S L L A E S P I D L V G P M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 43 End: 366 .......................................... L P L F C L K L L F I L F A L C T H R E L F D A T T D V P I R E E A T Y A I Q G L L H H L R H H L L Q T G V C F S V V M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 988 End: 1017 ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGATACTATTGGCAAGGTCAAGTATCAT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 45 HP_score: -2.4 Tail_Score: -3.43218 Start: 223 End: 246 Full_Region: acggctgatttcctc gaacgaca tacttgcc tggcgttc ttgatattccttctg ..............................................................................................................................................................................................................................gaacgacatacttgcctggcgttc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 44 HP_score: -3.3 Tail_Score: -2.87647 Start: 424 End: 439 Full_Region: atgtcgcactctggt cagta ccgata tactg tctcatctgctgaat .......................................................................................................................................................................................................................................................................................................................................................................................................................................cagtaccgatatactg................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 47 HP_score: -3.9 Tail_Score: -3.17219 Start: 472 End: 495 Full_Region: gtcgtatacagccac gctatcagc aaatc gcttgatggc ttcgtccaacttctg .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatcagcaaatcgcttgatggc......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 53 HP_score: -2.4 Tail_Score: -3.91084 Start: 1210 End: 1228 Full_Region: TTGGATACTGTTGTC GGACGT ATCGGTA ACGTTC TTTCCTTCGGACTGC .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGACGTATCGGTAACGTTC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: PI1654:PI1655|PI1654:PI1655:hypothetical protein:hypothetical protein:->->:1568019..1569818 1800 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Intra-Species Hit: Count: 1 Min: 1 Max: 1800 Len: 1800 Subject: pint_PI1654_PI1655|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1568019,1569818]|1800 HSP 1 e-value: 0.0 bit: 3568.0 Len: 1800 Query Start:1 Query End:1800 Subject Strand: POSITIVE Subject Start: 1 Subject End: 1800 aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca aacgaagatgagataaataaagaagagcaaaagaaacgcttagagcagcagagaaatgaagtgcaggctatgagtgaatatctcaagacttatggctcttttcagcagcaaaagcttgcgattgctgaagattatgcccaacagatagcggatatagatgcttcggaagtgagcgagacaacaaagaagtggcagaaagcaaagcttcagaaggaatatcaagaacgccaggcaagtatgtcgttcgaggaaatcagccgtggtatcgactggaatgcgctcttcagcggtgtaggcaatctgaccaaggagatgatgcaaccgatgatggagcagctgcgtgcctacacagaaactgacgactataggaacgcttcagccgatacacaacagaaagtgacggagctaattcagcagatgagacagtatatcggtactgaccagagtgcgacatggcagaagttggacgaagccatcaagcgatttgctgatagcgtggctgtatacgaccaagttaagaaagatgaagctgctgccgttgctgccatggaagcaggaaaggtaggactccgtgaagggagaatcagtaaggaaagatacgatgaacttgaagcacgtgctgaagagctggggagggcaacagtacaagcacgcgaggatatggactccttcggcaaagcattaaataggacatctgaagaagttgctaactttacgtctggattaacaacggctctcaataacgctaaggcatggcagggtgtagatggatacggaggcgtacagcagtcagtaggacaggttgatgcactcaaaggtacgcttgattctattcttccaaccatgggagacggcatagcaaagagtgttggcagtgctgtgtcaggagcaatgggaaatgcgttgtcctctcttggtggcacgatgtcgggcattctatcaagtggcataggaagtatggttggcattattgcacaaatacccagaatgatacttgaccttgccaatagtatcaaaagctttgttactggtgttctgaactcactgacagaacttatatctttgcgctggatagacgatcttgtaaatagcatcttggaagctatcggtaatcttatcaatgctatttttgacttgccagagaatttatttaaggtacttgagtctattatagtaaaaggtattggaggtttgttggatactgttgtcggacgtatcggtaacgttctttccttcggactgctcagtcataaaggtcccagcagctggtttactaacagtaacgaggaggaagtggcaaaatcaattgatcgcctgacaaaacgtaacgaactcctggagcaggcaattgaggacctaacggacgagatgaagacggcacgaggtgctacagctattcgcatatcacgcgatgcagagaaacttcagcgtgaaacgaacgagaactacaaacgtattgcacaggaacaggcaggctatcactcggcacatcatagtttcaacgcctactggaaagggtttagtcaggagcaaataaatcgtttcagttctcaaataggcagaaaatgggacggcaacttgtggaatctcactcccgaagaaatgaagatgttacgttccaatgtcgatatgtgggaaaagatccagaatacaggtaagggagactatggtggtcgcgtagctgataagcttaatgactacatcgcacaagccggcaagctgaaagaaataacagatgctctctacgagaacctgactacaacaacaaggcagaatgtctttgacgacttccttaactca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACGAAGAUGAGAUAAAUAAAGAAGAGCAAAAGAAACGCUUAGAGCAGCAGAGAAAUGAAGUGCAGGCUAUGAGUGAAUAUCUCAAGACUUAUGGCUCUUUUCAGCAGCAAAAGCUUGCGAUUGCUGAAGAUUAUGCCCAACAGAUAGCGGAUAUAGAUGCUUCGGAAGUGAGCGAGACAACAAAGAAGUGGCAGAAAGCAAAGCUUCAGAAGGAAUAUCAAGAACGCCAGGCAAGUAUGUCGUUCGAGGAAAUCAGCCGUGGUAUCGACUGGAAUGCGCUCUUCAGCGGUGUAGGCAAUCUGACCAAGGAGAUGAUGCAACCGAUGAUGGAGCAGCUGCGUGCCUACACAGAAACUGACGACUAUAGGAACGCUUCAGCCGAUACACAACAGAAAGUGACGGAGCUAAUUCAGCAGAUGAGACAGUAUAUCGGUACUGACCAGAGUGCGACAUGGCAGAAGUUGGACGAAGCCAUCAAGCGAUUUGCUGAUAGCGUGGCUGUAUACGACCAAGUUAAGAAAGAUGAAGCUGCUGCCGUUGCUGCCAUGGAAGCAGGAAAGGUAGGACUCCGUGAAGGGAGAAUCAGUAAGGAAAGAUACGAUGAACUUGAAGCACGUGCUGAAGAGCUGGGGAGGGCAACAGUACAAGCACGCGAGGAUAUGGACUCCUUCGGCAAAGCAUUAAAUAGGACAUCUGAAGAAGUUGCUAACUUUACGUCUGGAUUAACAACGGCUCUCAAUAACGCUAAGGCAUGGCAGGGUGUAGAUGGAUACGGAGGCGUACAGCAGUCAGUAGGACAGGUUGAUGCACUCAAAGGUACGCUUGAUUCUAUUCUUCCAACCAUGGGAGACGGCAUAGCAAAGAGUGUUGGCAGUGCUGUGUCAGGAGCAAUGGGAAAUGCGUUGUCCUCUCUUGGUGGCACGAUGUCGGGCAUUCUAUCAAGUGGCAUAGGAAGUAUGGUUGGCAUUAUUGCACAAAUACCCAGAAUGAUACUUGACCUUGCCAAUAGUAUCAAAAGCUUUGUUACUGGUGUUCUGAACUCACUGACAGAACUUAUAUCUUUGCGCUGGAUAGACGAUCUUGUAAAUAGCAUCUUGGAAGCUAUCGGUAAUCUUAUCAAUGCUAUUUUUGACUUGCCAGAGAAUUUAUUUAAGGUACUUGAGUCUAUUAUAGUAAAAGGUAUUGGAGGUUUGUUGGAUACUGUUGUCGGACGUAUCGGUAACGUUCUUUCCUUCGGACUGCUCAGUCAUAAAGGUCCCAGCAGCUGGUUUACUAACAGUAACGAGGAGGAAGUGGCAAAAUCAAUUGAUCGCCUGACAAAACGUAACGAACUCCUGGAGCAGGCAAUUGAGGACCUAACGGACGAGAUGAAGACGGCACGAGGUGCUACAGCUAUUCGCAUAUCACGCGAUGCAGAGAAACUUCAGCGUGAAACGAACGAGAACUACAAACGUAUUGCACAGGAACAGGCAGGCUAUCACUCGGCACAUCAUAGUUUCAACGCCUACUGGAAAGGGUUUAGUCAGGAGCAAAUAAAUCGUUUCAGUUCUCAAAUAGGCAGAAAAUGGGACGGCAACUUGUGGAAUCUCACUCCCGAAGAAAUGAAGAUGUUACGUUCCAAUGUCGAUAUGUGGGAAAAGAUCCAGAAUACAGGUAAGGGAGACUAUGGUGGUCGCGUAGCUGAUAAGCUUAAUGACUACAUCGCACAAGCCGGCAAGCUGAAAGAAAUAACAGAUGCUCUCUACGAGAACCUGACUACAACAACAAGGCAGAAUGUCUUUGACGACUUCCUUAACUCA ........((((............((((.........)))).(((..(((((((.((((.((((.((((((((((............)))))))))).(((((((((((((....))))...))))))))).((((((....(((.(((((((((...((((((....).)))))..........(((((.((.....))...))))).......)))))....(((((..((...((((((...((........((((((.((((((.(((((..((((((((((.((((....(((((((........))))..))).)))).))).))))).)).((((.((((..................)))).))))))))).))))))....((((..((((...(((...(((..((((((.....((((.((..((((((......(((..((((((((....(((....(((((((.(((.....)))..((((.(((.((....)).))).)))).....))))..)))....)))...))))))))...)))............((((......))))..))))))..))...)))).......((((.((...((((((((.(((...(..(....)..)...)))....(((........)))))))))))..)).))))......))))))..)))...)))....)))).))))........))))))........))....))))))))..)))))...........(((((((.((((.(((.....)))..)))))))).))).......))))....)))..(((((((....))))))).)))))).....(((((.((((..((((...((..(.((((((.(....((((((...((((...(((((((..((((((((..((..(((((((.(((.((..((((..((((((....))).)))))))))...))).)))))))..((((((......((.....(((((((((((...(..((((((..(..(((((.(((((((((((..(((((((((((..((....(((((((((...(((.((.....))..))).....)))))))))..))..((((..(((.....)))..)))).....))))))).)))).))))))))..))).)))))..)...((((((..((((((.......))))))...((((..((((....))))...))))...))))))..))))))..).)))))))))))..))..))))))........))..))))))))...(((..((...((((.((........)).))))((....))............))..)))..))))))).((...((((.......)))).)).)))).....))))))....(......).........).)))))).)..))...)))).)))).))))).)))).)))).........((((.((....)).(((((((((((..........((((..((.(((((......).))))..))..))))...((((((((.(((...(((((.....(((..((((((.......))))))..))).)))))..)))...........(((..((....))...(((((((...((((....))))...))))))).........))).(((..(((..(....)..))).)))..))))))))..)))))))).)))....))))......)))))).)..)))......)))) (-516.66) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGUUAAGGAAGUCGUCAAAGACAUUCUGCCUUGUUGUUGUAGUCAGGUUCUCGUAGAGAGCAUCUGUUAUUUCUUUCAGCUUGCCGGCUUGUGCGAUGUAGUCAUUAAGCUUAUCAGCUACGCGACCACCAUAGUCUCCCUUACCUGUAUUCUGGAUCUUUUCCCACAUAUCGACAUUGGAACGUAACAUCUUCAUUUCUUCGGGAGUGAGAUUCCACAAGUUGCCGUCCCAUUUUCUGCCUAUUUGAGAACUGAAACGAUUUAUUUGCUCCUGACUAAACCCUUUCCAGUAGGCGUUGAAACUAUGAUGUGCCGAGUGAUAGCCUGCCUGUUCCUGUGCAAUACGUUUGUAGUUCUCGUUCGUUUCACGCUGAAGUUUCUCUGCAUCGCGUGAUAUGCGAAUAGCUGUAGCACCUCGUGCCGUCUUCAUCUCGUCCGUUAGGUCCUCAAUUGCCUGCUCCAGGAGUUCGUUACGUUUUGUCAGGCGAUCAAUUGAUUUUGCCACUUCCUCCUCGUUACUGUUAGUAAACCAGCUGCUGGGACCUUUAUGACUGAGCAGUCCGAAGGAAAGAACGUUACCGAUACGUCCGACAACAGUAUCCAACAAACCUCCAAUACCUUUUACUAUAAUAGACUCAAGUACCUUAAAUAAAUUCUCUGGCAAGUCAAAAAUAGCAUUGAUAAGAUUACCGAUAGCUUCCAAGAUGCUAUUUACAAGAUCGUCUAUCCAGCGCAAAGAUAUAAGUUCUGUCAGUGAGUUCAGAACACCAGUAACAAAGCUUUUGAUACUAUUGGCAAGGUCAAGUAUCAUUCUGGGUAUUUGUGCAAUAAUGCCAACCAUACUUCCUAUGCCACUUGAUAGAAUGCCCGACAUCGUGCCACCAAGAGAGGACAACGCAUUUCCCAUUGCUCCUGACACAGCACUGCCAACACUCUUUGCUAUGCCGUCUCCCAUGGUUGGAAGAAUAGAAUCAAGCGUACCUUUGAGUGCAUCAACCUGUCCUACUGACUGCUGUACGCCUCCGUAUCCAUCUACACCCUGCCAUGCCUUAGCGUUAUUGAGAGCCGUUGUUAAUCCAGACGUAAAGUUAGCAACUUCUUCAGAUGUCCUAUUUAAUGCUUUGCCGAAGGAGUCCAUAUCCUCGCGUGCUUGUACUGUUGCCCUCCCCAGCUCUUCAGCACGUGCUUCAAGUUCAUCGUAUCUUUCCUUACUGAUUCUCCCUUCACGGAGUCCUACCUUUCCUGCUUCCAUGGCAGCAACGGCAGCAGCUUCAUCUUUCUUAACUUGGUCGUAUACAGCCACGCUAUCAGCAAAUCGCUUGAUGGCUUCGUCCAACUUCUGCCAUGUCGCACUCUGGUCAGUACCGAUAUACUGUCUCAUCUGCUGAAUUAGCUCCGUCACUUUCUGUUGUGUAUCGGCUGAAGCGUUCCUAUAGUCGUCAGUUUCUGUGUAGGCACGCAGCUGCUCCAUCAUCGGUUGCAUCAUCUCCUUGGUCAGAUUGCCUACACCGCUGAAGAGCGCAUUCCAGUCGAUACCACGGCUGAUUUCCUCGAACGACAUACUUGCCUGGCGUUCUUGAUAUUCCUUCUGAAGCUUUGCUUUCUGCCACUUCUUUGUUGUCUCGCUCACUUCCGAAGCAUCUAUAUCCGCUAUCUGUUGGGCAUAAUCUUCAGCAAUCGCAAGCUUUUGCUGCUGAAAAGAGCCAUAAGUCUUGAGAUAUUCACUCAUAGCCUGCACUUCAUUUCUCUGCUGCUCUAAGCGUUUCUUUUGCUCUUCUUUAUUUAUCUCAUCUUCGUU ((((.((((((((..((.(((((.....(((...(((((((.((.(((((.(((.....))).)))))............((((..(((...(((.(((((((......((((....))))..((((..........................((((((...............((((...(((((((((...((((((((....))))))))..............(((..(((((((.........))))).))..)))........(((((((..............((((((((((((......((((.(((((((((((((.............(((((.....)))))......((((((.((((((.((.((......)).))))))))...)))))).)))))..)).)))).))))))........(.((....)).).)))).)))))))).)))))))..))))))))))))).(((((((..(((((((.((((............(((((((.((...((((...))))..(((((..((((....)))).)))))...(.((((.......)))).).))))))))).......................((((............))))................)))))))))))((((((((((...(((.(((....))))))....))))))))))....))))))).)))))).(((((((.....((((((..........))))))..(((.(((((((((((.((......((((((((..((((((.......((((((........))))))....))))))))).)))))......((((((((.(.((..((((.((((((..((((((..(((........)))..((((................(((((....((((.......((((((.((..............((((((..((((.....))))...(((.....)))....))))))....(((......)))..)).)))))).....))))....)))))..(((((((((...........)))))))))....)))).)))))).......(((((((((.((((.(((.......((((((((.(....((((.......))))....)))))))))..........((((((........))).)))...........))).)))).......(((((.....)))))...)))))).)))..............)))))).)))).(((((..((((((((((....((...(((((..............)))))...)).....((.(((((......))))).))....))))))..))))...............((.((((((((((((((((((.........(((((....(((((((((.(((((((.........)))))))...((((........))))))))))))).))))).)))))).)).)))))))))))).)))))....................)).).))))))))..........)).))))))))).)).))).....)))))))....))))...............))))))))))...)))..)))).....)).))))))).))).((((..((.((.((((.(((......((..((((.......))))..))......))).))))...)).))...))))...))))).)).)))))))).....))))........ (-474.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 459 575 537 42 2 pint:PI1749|5end_conserved_hypothetical_protein_1671039..1671269_POSITIVE 459 790 743 63 18 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 455 677 631 53 4 pint:PI2013|5end_hypothetical_protein_1972329..1972559_POSITIVE 434 659 612 227 180 pint:PI0777|5end_predicted_TPR-repeat-containing_protein_713285..713515_POSITIVE 429 659 613 189 146 pint:PI1378|5end_GTP-binding_protein_lepA_1299904..1300134_POSITIVE 429 919 871 193 146 pint:PI0258|5end_conjugative_transposon_protein,_TraG_249722..249952_POSITIVE 422 1684 1642 60 6 pint:PI0950|5end_50S_ribosomal_protein_L31_883327..883557_POSITIVE 415 1720 1671 90 32 pint:PI0872|5end_hypothetical_protein_807371..807601_POSITIVE 414 578 532 39 2 pint:PI2147|5end_GTP-binding_protein_2093263..2093493_POSITIVE 413 180 135 200 153 pint:PI2109|5end_surface_antigen_BspA_2060553..2060783_POSITIVE 410 799 752 91 38 pint:PI0668|5end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_(peptidylprolyl_isomerase)_610216..610446_POSITIVE 402 1420 1371 122 52 pint:PI1690|5end_transcription_regulator;_possible_Fe2+/Zn2+_uptake_regulation_proteins_1604984..1605214_POSITIVE 401 530 482 77 17 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 401 789 744 198 161 pint:PI0733|5end_membrane_protein;_predicted_exporter_664389..664619_POSITIVE 397 947 901 55 6 pint:PI0042|5end_hypothetical_protein_42981..43211_POSITIVE 395 566 523 121 68 pint:PI0729|5end_integrase_661428..661658_POSITIVE 392 960 915 221 184 pint:PI1200|5end_carbon-nitrogen_hydrolase/beta-alanine_synthase_1115662..1115892_POSITIVE 389 800 751 201 144 pint:PI1038|5end_hypothetical_protein_958685..958915_POSITIVE 389 967 921 191 143 pint:PI0920|5end_xanthan_lyase_849688..849918_POSITIVE 385 896 853 190 147 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 401 789 744 96 59 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 397 947 901 150 101 pint:PI0043|3end_thiamine_phosphate_pyrophosphorylase_43156..43306_POSITIVE 395 566 523 121 68 pint:PI0728|3end_integrase_661508..661658_POSITIVE 390 511 472 142 100 pint:PI1490|3end_GTP_cyclohydrolase_I_1409127..1409277_POSITIVE 389 967 921 142 94 pint:PI0919|3end_conserved_hypothetical_protein_849719..849869_POSITIVE 382 790 745 66 17 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE 373 1520 1471 104 56 pint:PI0805|3end_conserved_hypothetical_protein_743807..743957_POSITIVE 371 790 743 96 51 pint:PI0053|3end_hypothetical_protein_55124..55274_POSITIVE 368 549 509 115 72 pint:PI0758|3end_hypothetical_protein_690506..690656_POSITIVE 367 360 311 100 37 pint:PI0425|3end_hypothetical_protein_389722..389872_POSITIVE 365 562 527 145 106 pint:PI1313|3end_possible_POT_family_(proton-dependent_oligopeptide_transport_family)_1231241..1231391_POSITIVE 359 950 902 117 52 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 358 800 758 59 9 pint:PI1801|3end_30S_ribosomal_protein_S9_1734412..1734562_POSITIVE 356 650 604 51 3 pint:PI1735|3end_conserved_hypothetical_protein_1654323..1654473_POSITIVE 353 784 744 110 65 pint:PI1273|3end_anthranilate_synthase_component_II_1192955..1193105_POSITIVE 353 1380 1337 48 5 pint:PI0953|3end_hypothetical_protein_885390..885540_POSITIVE 353 1380 1337 106 63 pint:PI0951|3end_hypothetical_protein_885448..885598_POSITIVE 353 288 241 96 52 pint:PI0624|3end_hypothetical_protein_574471..574621_POSITIVE 352 348 306 148 100 pint:PI1641|3end_hypothetical_protein_1558883..1559033_POSITIVE 352 348 306 148 100 pint:PI0347|3end_hypothetical_protein_321655..321805_POSITIVE