Origin IGS: taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atattactcttttactttccactcaatagttacaacggcattgagcttaccgctgcccttgcatatcgggcattcctttttataccgctcttgatactcgtcctcttgccagtggtacccatttccttgacaatatgggcaactatgtccttggctttcgaggcactctgtcattcgactacctggaatcattctccctggcgttatttccacaattcttttttctctactcatatccta Mask Tandem Repeat Region ================================================ taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Find is-nt database================================================ Query_seq: PI1629:PI1630|PI1629:PI1630:hypothetical protein:hypothetical protein:->->:1552027..1552266 240 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1629:PI1630|PI1629:PI1630:hypothetical protein:hypothetical protein:->->:1552027..1552266 240 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1629:PI1630|PI1629:PI1630:hypothetical protein:hypothetical protein:->->:1552027..1552266 240 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 95 End: 208 .............................................................................................. M V A H I V K E M G T T G K R T S I K S G I K R N A R Y A R A A V S S M P L ................................ Protein_Len: 60 Strand: + Start: 7 End: 234 ...... M S R E K R I V E I T P G R M I P G S R M T E C L E S Q G H S C P Y C Q G N G Y H W Q E D E Y Q E R Y K K E C P I C K G ...... Protein_Len: 59 Strand: - Start: 2 End: 178 . P Y S Y L F F F Q P F L A L S F S E L Y D F S L T G R F G L V Y N G M N D L F H T G S A L P R T D L A T Y F P I G S M .............................................................. Protein_Len: 60 Strand: - Start: 1 End: 240 T S H C L A E F A L S M T A W I T L S I P V V P L L V L I L L P I F L F A R Y A L A A T L E I G N Y S N L P F Y F L L M taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 107 End: 136 ..........................................................................................................CTTGCCAGTGGTACCCATTTCCTTGACAAT........................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1629:PI1630|PI1629:PI1630:hypothetical protein:hypothetical protein:->->:1552027..1552266 240 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Intra-Species Hit: Count: 1 Min: 1 Max: 240 Len: 240 Subject: pint_PI1629_PI1630|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1552027,1552266]|240 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:1 Query End:240 Subject Strand: POSITIVE Subject Start: 1 Subject End: 240 taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat taggatatgagtagagaaaaaagaattgtggaaataacgccagggagaatgattccaggtagtcgaatgacagagtgcctcgaaagccaaggacatagttgcccatattgtcaaggaaatgggtaccactggcaagaggacgagtatcaagagcggtataaaaaggaatgcccgatatgcaagggcagcggtaagctcaatgccgttgtaactattgagtggaaagtaaaagagtaatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGAUAUGAGUAGAGAAAAAAGAAUUGUGGAAAUAACGCCAGGGAGAAUGAUUCCAGGUAGUCGAAUGACAGAGUGCCUCGAAAGCCAAGGACAUAGUUGCCCAUAUUGUCAAGGAAAUGGGUACCACUGGCAAGAGGACGAGUAUCAAGAGCGGUAUAAAAAGGAAUGCCCGAUAUGCAAGGGCAGCGGUAAGCUCAAUGCCGUUGUAACUAUUGAGUGGAAAGUAAAAGAGUAAUAU ...(((((..((.............((((....)))).(((((((.......(((.(((((.((........)).))))).)))...............(((((((.((......)).))))))))).)))))......))..)))))...((((((((.........(((((.........)))))((.....))...))))))))...(((....(.(....).).....)))..... (-56.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUACUCUUUUACUUUCCACUCAAUAGUUACAACGGCAUUGAGCUUACCGCUGCCCUUGCAUAUCGGGCAUUCCUUUUUAUACCGCUCUUGAUACUCGUCCUCUUGCCAGUGGUACCCAUUUCCUUGACAAUAUGGGCAACUAUGUCCUUGGCUUUCGAGGCACUCUGUCAUUCGACUACCUGGAAUCAUUCUCCCUGGCGUUAUUUCCACAAUUCUUUUUUCUCUACUCAUAUCCUA ...........................(((..(((.(((....(((.....)))((((.........))))...............))).)))..))).........(((((.((.......((((.(((((..((((....))))(((((((.....))))).))..)))))...........)))).......)))))))...................................... (-42.24) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 159 111 210 164 pint:PI1887|5end_cell_division_protein_1829115..1829345_POSITIVE 362 83 42 230 181 pint:PI1620|5end__two-component_system_sensor_protein_1543956..1544186_POSITIVE 346 123 84 180 131 pint:PI0087|5end_conserved_hypothetical_protein_85700..85930_POSITIVE 344 209 173 155 108 pint:PI1522|5end_thioredoxin_M_1441646..1441876_POSITIVE 341 160 111 93 30 pint:PI0188|5end_phosphomethylpyrimidine_kinase_178433..178663_POSITIVE 338 186 141 220 171 pint:PI0021|5end_orotate_phosphoribosyltransferase_24939..25169_POSITIVE 331 175 131 60 16 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 330 159 112 187 137 pint:PI0321|5end_conserved_hypothetical_protein_302598..302828_POSITIVE 330 206 167 51 4 pint:PI0180|5end_TonB_protein_171905..172135_POSITIVE 329 200 164 75 27 pint:PI1727|5end__ATP-dependent_Clp_protease,_ATP-binding_subunit_1645680..1645910_POSITIVE 328 204 162 86 28 pint:PI0992|5end_RNA_polymerase_sigma-70_factor_916456..916686_POSITIVE 327 210 163 59 6 pint:PI1781|5end_membrane-associated_zinc_metalloprotease_1713074..1713304_POSITIVE 327 205 164 83 38 pint:PI0604|5end_O-succinylbenzoic_acid--CoA_ligase_550825..551055_POSITIVE 325 157 112 49 10 pint:PI1898|5end_S-adenosyl-methyltransferase_1841288..1841518_POSITIVE 323 115 72 65 15 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 321 223 181 43 5 pint:PI2117|5end_fumarate_reductase/succinate_dehydrogenase_flavoprotein_subunit,_C-terminal_2066017..2066247_POSITIVE 320 191 151 60 19 pint:PI0734|5end_ABC_transporter,_ATP-binding_/_permease_667052..667282_POSITIVE 319 208 163 206 159 pint:PI2048|5end_possible_two-component_system_sensor_histidine_kinase_2000705..2000935_POSITIVE 318 128 86 63 23 pint:PI1791|5end_conserved_hypothetical_protein_1718489..1718719_POSITIVE 318 128 86 55 15 pint:PI1788|5end_hypothetical_protein_1718481..1718711_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 195 151 138 93 pint:PI1543|3end_hypothetical_protein_1463281..1463431_POSITIVE 319 208 163 127 80 pint:PI2047|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino_pimelate--D-alanyl-D-alanyl_ligase_2000706..2000856_POSITIVE 315 78 33 109 61 pint:PI1029|3end_seryl-tRNA_synthetase_947205..947355_POSITIVE 315 75 38 151 103 pint:PI0556|3end_saccharopine_dehydrogenase_506160..506310_POSITIVE 314 208 168 98 49 pint:PI1789|3end_hypothetical_protein_1719037..1719187_POSITIVE 311 188 141 123 75 pint:PI1233|3end_conserved_hypothetical_protein;_possible__MazG_family_protein_1154247..1154397_POSITIVE 311 198 151 139 82 pint:PI1071|3end_DNA_topoisomerase_III_990226..990376_POSITIVE 308 230 185 101 54 pint:PI0827|3end_hypothetical_protein_764163..764313_POSITIVE 308 230 185 134 87 pint:PI0825|3end_hypothetical_protein_764196..764346_POSITIVE 306 111 77 150 117 pint:PI0086|3end_possible_uroporphyrinogen-III_synthase_85770..85920_POSITIVE 305 186 142 77 35 pint:PI1892|3end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_1835547..1835697_POSITIVE 301 209 164 101 53 pint:PI0398|3end_hypothetical_protein_363541..363691_POSITIVE 300 204 164 89 39 pint:PI0092|3end_regulatory_protein_RecX_89651..89801_POSITIVE 299 103 61 55 12 pint:PI1467|3end_conserved_hypothetical_protein_1393909..1394059_POSITIVE 298 209 164 115 79 pint:PI1942|3end_hypothetical_protein_1901269..1901419_POSITIVE 294 228 182 131 72 pint:PI0196|3end_hypothetical_protein_188643..188793_POSITIVE 293 210 165 139 99 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 291 210 164 144 86 pint:PI0822|3end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_762025..762175_POSITIVE 289 193 151 139 93 pint:PI1728|3end_ATP-dependent_Clp_protease,_proteolytic_subunit_1647658..1647808_POSITIVE 289 140 91 64 15 pint:PI0156|3end_conserved_hypothetical_protein_150913..151063_POSITIVE