Origin IGS: tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagttccaaatagaatccactctgccgatatattcgcatttgcgcaaactttcctcaagacttcataagaagggtttccttttcgtgtaccaacaatattttcaaccacagtggggcttactcctactgtctttgcaaatgctcttttgttattattgaatagtaccttgatgattgtctcaaatctctcatttatgctcatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatatctcacttatttaattaataaataattctttattttcgcccctttttcgtatctttgggcggtgtttaagattaaacacgttgcaaagataatacgcttttgcgaatattcaaaataaaaatgcgaatattttattcgcaaatgataatatttataaaatatgagcataaatgagagatttgagacaatcatcaaggtactattcaataataacaaaagagcatttgcaaagacagtaggagtaagccccactgtggttgaaaatattgttggtacacgaaaaggaaacccttcttatgaagtcttgaggaaagtttgcgcaaatgcgaatatatcggcagagtggattctatttggaactgagaaccagccagtaggtgattttatcaatattcgtaaagatcttacaatagtagctccaaaagagggggagcctgaagatggagaacataccgtaactacagtttttactaaaggcaaagccggatccaaaccttttcctgtgcagaaagcacaagacgcagacccatttcttaaagaattacttgacactatcaaagaacaagcagaggaaatcggacaactcaaggagcaagtccaccaactaactattgaaaaagaaaggcttgccgccaatgcccaatcttcaagcactgcgaacgtcggatagacgtctttagagttatcaagggaggacgttggtacgagcaagaataacgagaagatacaccataaccctccagtcccatccctttctcccccttttttctatcccccccctctttaacacccttgaaaataggaaagtgcttgatatagcgttatttaataaggtagagtttcaaaaacaggtggcatttaggggggggctatcggtggcataaatgcgaatatcattaaaaaagtagtattttccccccactgtatcataccccatcaaaacctatttttgcataaccacttttacaaaaatgcgtaaccactttgcataaccacgtgcataaccagtcctgaaaattgccttcgaatacatgtaaaaagggtgataaaaccactccgattttaccaccctcttaaacggcgttttaatgacgttctaagcccctttttcctca Mask Tandem Repeat Region ================================================ tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagttccaaatagaatccactctgccgatatattcgcatttgcgcaaactttcctcaagacttcataagaagggtttccttttcgtgtaccaacaatattttcaaccacagtggggcttactcctactgtctttgcaaatgctcttttgttattattgaatagtaccttgatgattgtctcaaatctctcatttatgctcatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Find is-nt database================================================ Query_seq: PI1621:PI1622|PI1621:PI1622:hypothetical protein:hypothetical protein:->->:1545801..1546921 1121 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagttccaaatagaatccactctgccgatatattcgcatttgcgcaaactttcctcaagacttcataagaagggtttccttttcgtgtaccaacaatattttcaaccacagtggggcttactcctactgtctttgcaaatgctcttttgttattattgaatagtaccttgatgattgtctcaaatctctcatttatgctcatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1621:PI1622|PI1621:PI1622:hypothetical protein:hypothetical protein:->->:1545801..1546921 1121 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagttccaaatagaatccactctgccgatatattcgcatttgcgcaaactttcctcaagacttcataagaagggtttccttttcgtgtaccaacaatattttcaaccacagtggggcttactcctactgtctttgcaaatgctcttttgttattattgaatagtaccttgatgattgtctcaaatctctcatttatgctcatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1621:PI1622|PI1621:PI1622:hypothetical protein:hypothetical protein:->->:1545801..1546921 1121 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagttccaaatagaatccactctgccgatatattcgcatttgcgcaaactttcctcaagacttcataagaagggtttccttttcgtgtaccaacaatattttcaaccacagtggggcttactcctactgtctttgcaaatgctcttttgttattattgaatagtaccttgatgattgtctcaaatctctcatttatgctcatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 758 Max: 952 Len: 195 Subject: UniRef90_Q11YF4 Cluster: Hypothetical protein; n=1; Cytophaga hutchinsonii ATCC 33406|Rep: Hypothetical protein - Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) HSP 1 e-value: 2.0E-6 bit: 56.6 Len: 195 Query Start:758 Query End:952 Subject Strand: null Subject Start: 3 Subject End: 67 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S I N E R F E T I I K V L F N N N K R A F A K T V G V S P T V V E N I V G T R K G N P S Y E V L R K V C A N - A N I S A E W I L F G ......................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S V N E R I K F L I E E L - N L N S R A F S L K L G V D P T I I H N I I S G R M S M P S Y V V L E K I V L T F D N V N A K W L L T G ......................................................................................................................................................................... tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 26 End: 169 ......................... M K T P F K R V V K S E W F Y H P F Y M Y S K A I F R T G Y A R G Y A K W L R I F V K V V M Q K ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 44 Strand: + Start: 55 End: 186 ...................................................... M G V V L S P F L H V F E G N F Q D W L C T W L C K V V T H F C K S G Y A K I G F D G V ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 176 End: 271 ............................................................................................................................................................................... M M G Y D T V G G K Y Y F F N D I R I Y A T D S P P L N A T C F .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 43 Strand: + Start: 299 End: 427 .......................................................................................................................................................................................................................................................................................................... M S S T F L F S R V L K R G G I E K R G R K G W D W R V M V Y L L V I L A R T N V L P ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 301 End: 504 ............................................................................................................................................................................................................................................................................................................ M K H F P I F K G V K E G G D R K K G E K G M G L E G Y G V S S R Y S C S Y Q R P P L I T L K T S I R R S Q C L K I G H ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 55 Strand: + Start: 599 End: 763 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M C F L H R K R F G S G F A F S K N C S Y G M F S I F R L P L F W S Y Y C K I F T N I D K I T Y W L V L S S K ...................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 390 End: 818 ..................................................................................................................................................................................................................................................................................................................................................................................................... M F S L F L L V P T S S L D N S K D V Y P T F A V L E D W A L A A S L S F S I V S W W T C S L S C P I S S A C S L I V S ............................................................................................................................................................................................................................................................................................................... Protein_Len: 56 Strand: + Start: 742 End: 909 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A G S Q F Q I E S T L P I Y S H L R K L S S R L H K K G F L F V Y Q Q Y F Q P Q W G L L L L S L Q M L F C Y Y .................................................................................................................................................................................................................... Protein_Len: 43 Strand: + Start: 785 End: 913 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M R I C A N F P Q D F I R R V S F S C T N N I F N H S G A Y S Y C L C K C S F V I I E ................................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 1007 End: 1102 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M F A K A Y Y L C N V F N L K H R P K I R K R G E N K E L F I N ................... Protein_Len: 60 Strand: + Start: 849 End: 1106 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M F S T T V G L T P T V F A N A L L L L L N S T L M I V S N L S F M L I F Y K Y Y H L R I K Y S H F Y F E Y S Q K R I I ............... Protein_Len: 35 Strand: - Start: 511 End: 615 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. N T S K S R S N D S K R Q K N K S L T L Y N K L F H T Q T K H K R C M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 449 End: 955 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................ A S I N A N A C V K R L V E Y S P N G K R T G V I N E V V T P S V G V T K A F A R K N N N F L V K I I T E F R E N I S M ...................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 235 End: 417 .......................................................................................................................................................................................................................................... V S L G G R F A V Q K Q F E V K N F L A I D L V K R N E L T N F L P P I S F L P L F P H S Q L T I T Y R R T I R A R V M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: - Start: 140 End: 244 ........................................................................................................................................... A N K Y F H N H L F L N Q H P I I C H P S F V V K K I I N A N I G G M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 42 Strand: - Start: 2 End: 127 . L F F P S L V D N F R R K L P H Y F R L P K I V R K V H I R L C N E P S T I C T T M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 59 HP_score: -6.9 Tail_Score: -3.0264 Start: 45 End: 75 Full_Region: gaatacatgtaaaaa gggtgataaaacc actcc gattttaccaccc tcttaaacggcgttt ............................................gggtgataaaaccactccgattttaccaccc...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -12.0 Tail_Score: -4.74453 Start: 45 End: 75 Full_Region: AAACGCCGTTTAAGA GGGTGGTAAAATCG GAG TGGTTTTATCACCC TTTTTACATGTATTC ............................................GGGTGGTAAAATCGGAGTGGTTTTATCACCC...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 49 HP_score: -2.2 Tail_Score: -3.69354 Start: 441 End: 457 Full_Region: tcttcaagcactgcg aacgtc ggata gacgtc tttagagttatcaag ........................................................................................................................................................................................................................................................................................................................................................................................................................................................aacgtcggatagacgtc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 48 HP_score: -2.8 Tail_Score: -3.59087 Start: 860 End: 884 Full_Region: AACAATATTTTCAAC CACAGTGGGG CTTAC TCCTACTGTC TTTGCAAATGCTCTT ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CACAGTGGGGCTTACTCCTACTGTC............................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 56 HP_score: -5.4 Tail_Score: -3.36991 Start: 862 End: 882 Full_Region: CAATATTTTCAACCA CAGTGGGG CTTAC TCCTACTG TCTTTGCAAATGCTC .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CAGTGGGGCTTACTCCTACTG............................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 41 HP_score: -2.5 Tail_Score: -2.73468 Start: 862 End: 882 Full_Region: gagcatttgcaaaga cagtagg agtaagc cccactg tggttgaaaatattg .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cagtaggagtaagccccactg............................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -10.4 Tail_Score: -5.48213 Start: 976 End: 994 Full_Region: ATAAATATTATCATT TGCGAATA AAA TATTCGCA TTTTTATTTTGAATA ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGCGAATAAAATATTCGCA............................................................................................................................... Find igs database================================================ Query_seq: PI1621:PI1622|PI1621:PI1622:hypothetical protein:hypothetical protein:->->:1545801..1546921 1121 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Intra-Species Hit: Count: 2 Min: 1 Max: 1121 Len: 1121 Subject: pint_PI1621_PI1622|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1545801,1546921]|1121 HSP 1 e-value: 0.0 bit: 1405.0 Len: 757 Query Start:1 Query End:757 Subject Strand: POSITIVE Subject Start: 1 Subject End: 757 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagnnnnnnnntaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagannnnnnnnatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagt............................................................................................................................................................................................................................................................................................................................................................................ tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtctatccgacgttcgcagtgcttgaagattgggcattggcggcaagcctttctttttcaatagttagttggtggacttgctccttgagttgtccgatttcctctgcttgttctttgatagtgtcaagtaattctttaagaaatgggtctgcgtcttgtgctttctgcacaggaaaaggtttggatccggctttgcctttagtaaaaactgtagttacggtatgttctccatcttcaggctccccctcttttggagctactattgtaagatctttacgaatattgataaaatcacctactggctggttctcagt............................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 2.0E-90 bit: 335.0 Len: 169 Query Start:953 Query End:1121 Subject Strand: POSITIVE Subject Start: 953 Subject End: 1121 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catattttataaatattatcatttgcgaataaaatattcgcatttttattttgaatattcgcaaaagcgtattatctttgcaacgtgtttaatcttaaacaccgcccaaagatacgaaaaaggggcgaaaataaagaattatttattaattaaataagtgagatatatt Subject: pint_PI0327_PI0328|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[309168,309615]|448 HSP 1 e-value: 0.0 bit: 729.0 Len: 448 Query Start:1 Query End:448 Subject Strand: POSITIVE Subject Start: 1 Subject End: 448 tgaggaaaaaggggcttagaacgtcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcaggactggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgatggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagnnnnnnnntaaatgccacctgtttttgaaactctaccttattaaataacgctatatcaagcactttcctattttcaagggtgttaaagannnnnnnnatagaaaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattcttgctcgtaccaacgtcctcccttgataactctaaagacgtcta................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tgaggaaaagggggcttagaacggcattaaaacgccgtttaagagggtggtaaaatcggagtggttttatcaccctttttacatgtattcgaaggcaattttcagggctggttatgcacgtggttatgcaaagtggttacgcatttttgtaaaagtggttatgcaaaaataggttttgacggggtatgatacagtggggggaaaatactacttttttaatgatattcgcatttatgccaccgatagcccccccctaaatgccaactgtttttgaaactctaccttattaaataaggctatatcaagcactttcctattttcaagggtgttaaagaggggggggatagagaaaagggggagaaagggatgggactggagggttatggtgtatcttctcgttattctttctcgtaccaacgtcctcccttgataactctaaagacgtcta................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGGAAAAAGGGGCUUAGAACGUCAUUAAAACGCCGUUUAAGAGGGUGGUAAAAUCGGAGUGGUUUUAUCACCCUUUUUACAUGUAUUCGAAGGCAAUUUUCAGGACUGGUUAUGCACGUGGUUAUGCAAAGUGGUUACGCAUUUUUGUAAAAGUGGUUAUGCAAAAAUAGGUUUUGAUGGGGUAUGAUACAGUGGGGGGAAAAUACUACUUUUUUAAUGAUAUUCGCAUUUAUGCCACCGAUAGCCCCCCCCUAAAUGCCACCUGUUUUUGAAACUCUACCUUAUUAAAUAACGCUAUAUCAAGCACUUUCCUAUUUUCAAGGGUGUUAAAGAGGGGGGGGAUAGAAAAAAGGGGGAGAAAGGGAUGGGACUGGAGGGUUAUGGUGUAUCUUCUCGUUAUUCUUGCUCGUACCAACGUCCUCCCUUGAUAACUCUAAAGACGUCUAUCCGACGUUCGCAGUGCUUGAAGAUUGGGCAUUGGCGGCAAGCCUUUCUUUUUCAAUAGUUAGUUGGUGGACUUGCUCCUUGAGUUGUCCGAUUUCCUCUGCUUGUUCUUUGAUAGUGUCAAGUAAUUCUUUAAGAAAUGGGUCUGCGUCUUGUGCUUUCUGCACAGGAAAAGGUUUGGAUCCGGCUUUGCCUUUAGUAAAAACUGUAGUUACGGUAUGUUCUCCAUCUUCAGGCUCCCCCUCUUUUGGAGCUACUAUUGUAAGAUCUUUACGAAUAUUGAUAAAAUCACCUACUGGCUGGUUCUCAGUUCCAAAUAGAAUCCACUCUGCCGAUAUAUUCGCAUUUGCGCAAACUUUCCUCAAGACUUCAUAAGAAGGGUUUCCUUUUCGUGUACCAACAAUAUUUUCAACCACAGUGGGGCUUACUCCUACUGUCUUUGCAAAUGCUCUUUUGUUAUUAUUGAAUAGUACCUUGAUGAUUGUCUCAAAUCUCUCAUUUAUGCUCAUAUUUUAUAAAUAUUAUCAUUUGCGAAUAAAAUAUUCGCAUUUUUAUUUUGAAUAUUCGCAAAAGCGUAUUAUCUUUGCAACGUGUUUAAUCUUAAACACCGCCCAAAGAUACGAAAAAGGGGCGAAAAUAAAGAAUUAUUUAUUAAUUAAAUAAGUGAGAUAUAUU ((((((((..((((((.(((((((((..........((..(((((((((((((((((.....))))))))))))))))).))..(((((((...(((....(((....)))...)))....((((.((((((((((((..(((((((((.(((.((((.....(((((((((((........(((((.....((.((((((................((((.......))))..............)))))).))..)))))))))))))))).....)))).............(((......))).........))).))))))))).((((((((((.(((((((....((((..(..((((((..(((((((.((((.((..((........))..))....))))...)))..))))))))))..)..)))).......)))))))............((((((((((.(((((..(.((((((((((((((.((((...((..((((((..(((((.......)))))..))))))..)).((((((..((.....))...)))))).........)))).)))......((((((((.....)))))))).))))))))...))).)..))))).........(((((....))))).........)))))))))).))))))))))...))))))).))))).))))....)))))))................))))..))))).))))))...((((......))))..........(((....))).....)))))))).(((.((((....(((((...((.((((.(((..((((((((((.(((..(((((((((.....)))))))))..))).))))).....)))))..))))))).)).))))).))))..))).......((((((((((......(((..(((((((...((...((((((((...))))))))...............(((((.....((((...((((((....(((((((....)))))))....))))))))))........)))))......))..))))))).)))...))))))))))...... (-313.73) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUAUCUCACUUAUUUAAUUAAUAAAUAAUUCUUUAUUUUCGCCCCUUUUUCGUAUCUUUGGGCGGUGUUUAAGAUUAAACACGUUGCAAAGAUAAUACGCUUUUGCGAAUAUUCAAAAUAAAAAUGCGAAUAUUUUAUUCGCAAAUGAUAAUAUUUAUAAAAUAUGAGCAUAAAUGAGAGAUUUGAGACAAUCAUCAAGGUACUAUUCAAUAAUAACAAAAGAGCAUUUGCAAAGACAGUAGGAGUAAGCCCCACUGUGGUUGAAAAUAUUGUUGGUACACGAAAAGGAAACCCUUCUUAUGAAGUCUUGAGGAAAGUUUGCGCAAAUGCGAAUAUAUCGGCAGAGUGGAUUCUAUUUGGAACUGAGAACCAGCCAGUAGGUGAUUUUAUCAAUAUUCGUAAAGAUCUUACAAUAGUAGCUCCAAAAGAGGGGGAGCCUGAAGAUGGAGAACAUACCGUAACUACAGUUUUUACUAAAGGCAAAGCCGGAUCCAAACCUUUUCCUGUGCAGAAAGCACAAGACGCAGACCCAUUUCUUAAAGAAUUACUUGACACUAUCAAAGAACAAGCAGAGGAAAUCGGACAACUCAAGGAGCAAGUCCACCAACUAACUAUUGAAAAAGAAAGGCUUGCCGCCAAUGCCCAAUCUUCAAGCACUGCGAACGUCGGAUAGACGUCUUUAGAGUUAUCAAGGGAGGACGUUGGUACGAGCAAGAAUAACGAGAAGAUACACCAUAACCCUCCAGUCCCAUCCCUUUCUCCCCCUUUUUUCUAUCCCCCCCCUCUUUAACACCCUUGAAAAUAGGAAAGUGCUUGAUAUAGCGUUAUUUAAUAAGGUAGAGUUUCAAAAACAGGUGGCAUUUAGGGGGGGGCUAUCGGUGGCAUAAAUGCGAAUAUCAUUAAAAAAGUAGUAUUUUCCCCCCACUGUAUCAUACCCCAUCAAAACCUAUUUUUGCAUAACCACUUUUACAAAAAUGCGUAACCACUUUGCAUAACCACGUGCAUAACCAGUCCUGAAAAUUGCCUUCGAAUACAUGUAAAAAGGGUGAUAAAACCACUCCGAUUUUACCACCCUCUUAAACGGCGUUUUAAUGACGUUCUAAGCCCCUUUUUCCUCA ......((((((((((.......(((((((....((((((((.............((((((((((((.((((((....)))))))))).))))))))...(((....))).......))))))))..((((((((...))))))))........))))))).....)))))......))))).....(((((..((((....((((((..(((((.((((......((....))....(((((.((.(....))).))))).))))....))))).))))))..(((..(....)..)))..)))).)))))((((((((...(.((..(((((.........(((((((((..((((((.((..(((.....)))))))))))...(((((...(((((((((...((.((((....(((((...(((((((((((((......(((((.((................((((((.((...(((((.(((............((((((.(((.....((.......)).......................((((.....)))).......))).))))))..((((..(((...)))...))))........................))))))))(((((((.((..((((..(((.(((.(.(((((.....))))).).))).)))...))))..)).)))))))...)).))))))...(((................)))...))))))).....))))))))))))).....((((((((......((((.((((((..........(((......))).((((((....))))))..)))))).....))))......)))))))))))))..)))).))...)))))))))...))))).....(((((((........((((...........(((((.....)))))............)))))))))))....))))))))).........))))).....(((...(((((.(((......((....))...((((((.(((((.(.....).))))).)))))).......))))))))...))).......)))...)))))))). (-259.66) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 453 487 441 140 87 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 434 559 513 170 121 pint:PI0052|5end_hypothetical_protein_52312..52542_POSITIVE 425 639 593 192 132 pint:PI0092|5end_regulatory_protein_RecX_89080..89310_POSITIVE 406 489 442 209 163 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 371 579 531 226 168 pint:PI1671|5end_hypothetical_protein_1586397..1586627_POSITIVE 371 640 591 59 2 pint:PI0379|5end_conserved_hypothetical_protein_349872..350102_POSITIVE 367 369 324 93 45 pint:PI1755|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_1681930..1682160_POSITIVE 364 527 482 138 84 pint:PI0383|5end_conserved_hypothetical_protein;_possible_Zn-dependent_hydrolase_352153..352383_POSITIVE 361 255 221 120 76 pint:PI2162|5end_conserved_hypothetical_protein_2108796..2109026_POSITIVE 360 491 454 65 28 pint:PI1145|5end_50S_ribosomal_protein_L35_1072520..1072750_POSITIVE 357 425 383 104 60 pint:PI2005|5end_potassium_uptake_protein_1962950..1963180_POSITIVE 346 378 337 59 5 pint:PI0608|5end_isochorismate_synthase_555487..555717_POSITIVE 343 569 521 200 157 pint:PI2125|5end_D-tyrosyl-tRNA(Tyr)_deacylase_2074450..2074680_POSITIVE 340 490 443 46 3 pint:PI1424|5end_probable_peptidase_family_M48_1343149..1343379_POSITIVE 338 497 451 228 182 pint:PI1981|5end_hypothetical_protein_1937897..1938127_POSITIVE 335 465 421 218 165 pint:PI1735|5end_conserved_hypothetical_protein_1653428..1653658_POSITIVE 333 628 583 61 17 pint:PI1468|5end_conserved_hypothetical_protein;_probable_TPR_domain_protein_1393900..1394130_POSITIVE 333 488 446 192 149 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 333 795 764 69 37 pint:PI0035|5end_NAD-dependent_protein_deacetylase,_SIR2_family_37873..38103_POSITIVE 332 640 591 130 78 pint:PI1770|5end_4-alpha-glucanotransferase_1697336..1697566_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 397 79 41 50 12 pint:PI1621|3end_hypothetical_protein_1545740..1545890_POSITIVE 397 79 41 50 12 pint:PI0327|3end_hypothetical_protein_309107..309257_POSITIVE 389 496 456 149 111 pint:PI0242|3end_hypothetical_protein_240227..240377_POSITIVE 367 369 324 93 45 pint:PI1754|3end_conserved_hypothetical_protein_1682010..1682160_POSITIVE 357 425 383 78 34 pint:PI2004|3end_1-deoxy-D-xylulose_5-phosphate_synthase_1963004..1963154_POSITIVE 354 397 354 143 93 pint:PI0221|3end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_217796..217946_POSITIVE 351 489 444 65 18 pint:PI1943|3end_hypothetical_protein_1902206..1902356_POSITIVE 348 380 333 123 71 pint:PI2095|3end_hypothetical_protein_2050216..2050366_POSITIVE 348 493 454 133 85 pint:PI1884|3end_conserved_hypothetical_protein_1827457..1827607_POSITIVE 347 428 388 66 27 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 341 504 467 91 52 pint:PI0301|3end_probable_transposase_285982..286132_POSITIVE 338 635 595 103 62 pint:PI2117|3end_fumarate_reductase/succinate_dehydrogenase_flavoprotein_subunit,_C-terminal_2066684..2066834_POSITIVE 337 460 416 114 67 pint:PI1558|3end_conserved_hypothetical_protein_1476106..1476256_POSITIVE 330 49 2 93 39 pint:PI0129|3end_hypothetical_protein_121941..122091_POSITIVE 325 492 451 53 8 pint:PI1821|3end_possible_thioredoxin_family_protein_1761517..1761667_POSITIVE 323 487 441 124 64 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 323 640 592 150 91 pint:PI1650|3end_N-acetylmuramoyl-L-alanine_amidase_1563901..1564051_POSITIVE 319 497 451 85 28 pint:PI2157|3end_xanthosine_triphosphate_pyrophosphatase,_HAM1_family_2104457..2104607_POSITIVE 318 369 334 65 23 pint:PI1512|3end_hypothetical_protein_1429893..1430043_POSITIVE 318 367 322 54 4 pint:PI1444|3end_protease_ClpB_1368137..1368287_POSITIVE