Origin IGS: atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatgcggaggaactcttgtcctactgcgccgctggcacctacgattgctactttcattatccttatatttattgttttgatgaggctacgcttgcccaataattgtcttgatgaccaacgtggagcgtaaagcccatatagttttcaattgcaaaaatacaaaaaactctctactatccctactcaacccataactttttgctatctttgcagatattttaatcatatcggat Mask Tandem Repeat Region ================================================ atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Find is-nt database================================================ Query_seq: PI1610:PI1611|PI1610:PI1611:Na+/H+ anti-porter:aspartate-semialdehyde dehydrogenase:->->:1538063..1538295 233 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1610:PI1611|PI1610:PI1611:Na+/H+ anti-porter:aspartate-semialdehyde dehydrogenase:->->:1538063..1538295 233 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1610:PI1611|PI1610:PI1611:Na+/H+ anti-porter:aspartate-semialdehyde dehydrogenase:->->:1538063..1538295 233 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 8 End: 145 ....... M I K I S A K I A K S Y G L S R D S R E F F V F L Q L K T I W A L R S T L V I K T I I G Q A ........................................................................................ Protein_Len: 33 Strand: + Start: 135 End: 233 ...................................................................................................................................... M G K R S L I K T I N I R I M K V A I V G A S G A V G Q E F L R I Protein_Len: 42 Strand: - Start: 107 End: 232 .......................................................................................................... A G R Q D D L C N N P L R L R M L V I F I L I I F T A I T P A L P A T P C S N R R M . Protein_Len: 31 Strand: - Start: 43 End: 135 .......................................... P N L L S L L S N K T N K C N F V I H A K R E V N T M L V I M .................................................................................................. atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 57 End: 86 ........................................................ATTGCAAAAATACAAAAAACTCTCTACTAT................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1610:PI1611|PI1610:PI1611:Na+/H+ anti-porter:aspartate-semialdehyde dehydrogenase:->->:1538063..1538295 233 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Intra-Species Hit: Count: 1 Min: 1 Max: 233 Len: 233 Subject: pint_PI1610_PI1611|Na+/H+ anti-porter:aspartate-semialdehyde dehydrogenase|POSITIVE:POSITIVE|[1538063,1538295]|233 HSP 1 e-value: 1.0E-129 bit: 462.0 Len: 233 Query Start:1 Query End:233 Subject Strand: POSITIVE Subject Start: 1 Subject End: 233 atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata atccgatatgattaaaatatctgcaaagatagcaaaaagttatgggttgagtagggatagtagagagttttttgtatttttgcaattgaaaactatatgggctttacgctccacgttggtcatcaagacaattattgggcaagcgtagcctcatcaaaacaataaatataaggataatgaaagtagcaatcgtaggtgccagcggcgcagtaggacaagagttcctccgcata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCCGAUAUGAUUAAAAUAUCUGCAAAGAUAGCAAAAAGUUAUGGGUUGAGUAGGGAUAGUAGAGAGUUUUUUGUAUUUUUGCAAUUGAAAACUAUAUGGGCUUUACGCUCCACGUUGGUCAUCAAGACAAUUAUUGGGCAAGCGUAGCCUCAUCAAAACAAUAAAUAUAAGGAUAAUGAAAGUAGCAAUCGUAGGUGCCAGCGGCGCAGUAGGACAAGAGUUCCUCCGCAUA ((((..((((.(((...(((((....)))))...........((..((((...............(((((((((((....))))...)))))))....(((((..((((((((.....(((.....)))......)))...))))))))))..))))..)).))).)))).))))..........((........(((((...)))))....((((....))))....))... (-46.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUGCGGAGGAACUCUUGUCCUACUGCGCCGCUGGCACCUACGAUUGCUACUUUCAUUAUCCUUAUAUUUAUUGUUUUGAUGAGGCUACGCUUGCCCAAUAAUUGUCUUGAUGACCAACGUGGAGCGUAAAGCCCAUAUAGUUUUCAAUUGCAAAAAUACAAAAAACUCUCUACUAUCCCUACUCAACCCAUAACUUUUUGCUAUCUUUGCAGAUAUUUUAAUCAUAUCGGAU ...((((((((.(....))))).)))).(((.((........((..((((...((((((.....(((.....)))..))))))(((((((((...(((......(((.....))).....))))))))..))))....))))..))..(((((((.(((((((((..............................)))))).))).)))))))............)).))).. (-43.61) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 225 182 75 21 pint:PI1887|5end_cell_division_protein_1829115..1829345_POSITIVE 385 226 181 187 134 pint:PI2034|5end_conserved_hypothetical_protein_1987573..1987803_POSITIVE 355 230 185 210 160 pint:PI0251|5end_hypothetical_protein_247551..247781_POSITIVE 353 226 182 178 117 pint:PI1754|5end_conserved_hypothetical_protein_1681202..1681432_POSITIVE 349 229 183 197 162 pint:PI1603|5end_hypothetical_protein_1533779..1534009_POSITIVE 340 217 173 102 53 pint:PI1890|5end_cell_division_protein_FtsW_1832886..1833116_POSITIVE 339 209 161 179 125 pint:PI0641|5end_conserved_hypothetical_protein_583791..584021_POSITIVE 339 227 187 70 36 pint:PI0581|5end_conserved_hypothetical_protein_525055..525285_POSITIVE 336 230 181 105 60 pint:PI1618|5end_hypothetical_protein_1542670..1542900_POSITIVE 333 229 189 177 135 pint:PI0465|5end_50S_ribosomal_protein_L22_435306..435536_POSITIVE 332 150 107 185 134 pint:PI1684|5end_dimethyladenosine_transferase_1601073..1601303_POSITIVE 332 227 186 155 114 pint:PI1439|5end_hypothetical_protein_1360009..1360239_POSITIVE 331 230 182 199 135 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 331 210 167 130 84 pint:PI0544|5end_hypoxanthine-guanine_phosphoribosyltransferase_(hypoxanthine_phosphoribosyltransferase)_495645..495875_POSITIVE 329 230 187 59 5 pint:PI1647|5end_hypothetical_protein_1561564..1561794_POSITIVE 323 230 183 71 12 pint:PI0107|5end_2-methylthioadenine_synthetase_103280..103510_POSITIVE 319 230 192 50 8 pint:PI1985|5end_hypothetical_protein_1940291..1940521_POSITIVE 319 230 185 209 146 pint:PI0301|5end_probable_transposase_285672..285902_POSITIVE 316 230 182 58 14 pint:PI1933|5end_hypothetical_protein_1879925..1880155_POSITIVE 313 215 171 223 154 pint:PI0646|5end_hypothetical_protein_586583..586813_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 230 191 135 87 pint:PI1973|3end_conserved_hypothetical_protein_1931171..1931321_POSITIVE 372 215 173 50 5 pint:PI1743|3end_stationary-phase_survival_protein_1664930..1665080_POSITIVE 343 229 183 57 8 pint:PI1815|3end_polyphosphate-selective_porin_O_1754510..1754660_POSITIVE 339 209 161 129 75 pint:PI0640|3end_conserved_hypothetical_protein_583821..583971_POSITIVE 339 227 187 149 115 pint:PI0582|3end_hypothetical_protein_525214..525364_POSITIVE 333 229 189 142 100 pint:PI0464|3end_30S_ribosomal_protein_S19_435351..435501_POSITIVE 331 210 167 127 81 pint:PI0543|3end_adenylate_kinase_495722..495872_POSITIVE 326 227 181 149 101 pint:PI1237|3end_ATP-dependent_DNA_helicase_1161480..1161630_POSITIVE 320 230 181 148 92 pint:PI1891|3end_hypothetical_protein_1835917..1836067_POSITIVE 319 67 21 55 4 pint:PI2123|3end_adenine_phosphoribosyltransferase_2072324..2072474_POSITIVE 313 149 106 137 85 pint:PI2073|3end_mobilization_protein_2033487..2033637_POSITIVE 313 215 171 129 60 pint:PI0644|3end_dnaJ_protein_586569..586719_POSITIVE 309 220 172 84 34 pint:PI1822|3end_ABC_transporter,_ATP-binding_/_permease_1763265..1763415_POSITIVE 305 230 194 42 2 pint:PI0354|3end_hypothetical_protein_326241..326391_POSITIVE 301 226 182 78 39 pint:PI0925|3end_ribosomal_protein_L11_methyltransferase_856638..856788_POSITIVE 296 230 183 134 65 pint:PI1395|3end_hypothetical_protein_1314407..1314557_POSITIVE 296 140 91 66 8 pint:PI1187|3end_conserved_hypothetical_protein_1107245..1107395_POSITIVE 296 230 191 150 115 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 295 230 182 93 49 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 293 230 185 57 8 pint:PI1642|3end_conserved_hypothetical_protein_1560439..1560589_POSITIVE