Origin IGS: tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agcatcgctgcccaacagtaattctgttcttgaaaattgctctttcataccttactaaattcctttgtgggtgcaaagttaagataacattcccataaaatggcagtagtttctgtttttatgttagcttttttctttacctttgcaagcgatgtgcggagtttgcacattattataaatgtggatttaaa Mask Tandem Repeat Region ================================================ tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Find is-nt database================================================ Query_seq: PI1585:PI1586|PI1585:PI1586:ampG permease:possible HesA/MoeB/ThiF family protein:->->:1514321..1514511 191 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1585:PI1586|PI1585:PI1586:ampG permease:possible HesA/MoeB/ThiF family protein:->->:1514321..1514511 191 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1585:PI1586|PI1585:PI1586:ampG permease:possible HesA/MoeB/ThiF family protein:->->:1514321..1514511 191 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 16 End: 144 ............... M I M C K L R T S L A K V K K K A N I K T E T T A I L W E C Y L N F A P T K E F S K V ............................................... Protein_Len: 35 Strand: + Start: 86 End: 190 ..................................................................................... M P F Y G N V I L T L H P Q R N L V R Y E R A I F K N R I T V G Q R C . Protein_Len: 30 Strand: - Start: 3 End: 92 .. L D V N I I I H L S R V D S A F T F F F A L M F V S V V A M ................................................................................................... tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1585:PI1586|PI1585:PI1586:ampG permease:possible HesA/MoeB/ThiF family protein:->->:1514321..1514511 191 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Intra-Species Hit: Count: 1 Min: 1 Max: 191 Len: 191 Subject: pint_PI1585_PI1586|ampG permease:possible HesA/MoeB/ThiF family protein|POSITIVE:POSITIVE|[1514321,1514511]|191 HSP 1 e-value: 1.0E-104 bit: 379.0 Len: 191 Query Start:1 Query End:191 Subject Strand: POSITIVE Subject Start: 1 Subject End: 191 tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct tttaaatccacatttataataatgtgcaaactccgcacatcgcttgcaaaggtaaagaaaaaagctaacataaaaacagaaactactgccattttatgggaatgttatcttaactttgcacccacaaaggaatttagtaaggtatgaaagagcaattttcaagaacagaattactgttgggcagcgatgct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAAAUCCACAUUUAUAAUAAUGUGCAAACUCCGCACAUCGCUUGCAAAGGUAAAGAAAAAAGCUAACAUAAAAACAGAAACUACUGCCAUUUUAUGGGAAUGUUAUCUUAACUUUGCACCCACAAAGGAAUUUAGUAAGGUAUGAAAGAGCAAUUUUCAAGAACAGAAUUACUGUUGGGCAGCGAUGCU ........((((((......))))))...........(((((((.((.........(((((..(((..(((((((.(((......)))...))))))).......(((((((.(((((......)))))........)))))))......)))..)))))...(((((.....)))))..))))))))).. (-39.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCAUCGCUGCCCAACAGUAAUUCUGUUCUUGAAAAUUGCUCUUUCAUACCUUACUAAAUUCCUUUGUGGGUGCAAAGUUAAGAUAACAUUCCCAUAAAAUGGCAGUAGUUUCUGUUUUUAUGUUAGCUUUUUUCUUUACCUUUGCAAGCGAUGUGCGGAGUUUGCACAUUAUUAUAAAUGUGGAUUUAAA .(((((((((((((..((.((((..((...(((((.......)))))......))..)))).))...)))))((((((..((((.........(((((((..((((......)))))))))))..........))))...)))))).))))))))...(((((..((((((......)))))))))))... (-44.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 351 70 21 152 96 pint:PI0734|5end_ABC_transporter,_ATP-binding_/_permease_667052..667282_POSITIVE 282 55 21 221 180 pint:PI1594|5end_MotA/TolQ/ExbB_proton_channel_1523528..1523758_POSITIVE 282 189 141 61 3 pint:PI1334|5end_ferrodoxin_1251404..1251634_POSITIVE 279 67 23 84 43 pint:PI1783|5end_possible_4-diphosphocytidyl-2c-methyl-D-erythritol_synthase_1715362..1715592_POSITIVE 271 69 30 105 54 pint:PI0189|5end_integrase_179623..179853_POSITIVE 268 124 81 49 7 pint:PI1390|5end_hypothetical_protein_1311154..1311384_POSITIVE 265 58 20 144 104 pint:PI1769|5end_hypothetical_protein_1696507..1696737_POSITIVE 261 65 21 82 28 pint:PI1086|5end_pyruvate:ferredoxin_oxidoreductase_1006602..1006832_POSITIVE 261 190 141 200 157 pint:PI0170|5end_hypothetical_protein_163305..163535_POSITIVE 261 190 141 59 16 pint:PI0167|5end_hypothetical_protein_163164..163394_POSITIVE 257 67 23 178 131 pint:PI1549|5end_conserved_hypothetical_protein_1467021..1467251_POSITIVE 255 57 24 177 130 pint:PI0346|5end_hypothetical_protein_320834..321064_POSITIVE 253 190 141 63 16 pint:PI0598|5end_hypothetical_protein_547061..547291_POSITIVE 252 67 23 90 34 pint:PI1090|5end_tRNA_uracil_5-methyltransferase_1014366..1014596_POSITIVE 251 143 103 129 86 pint:PI1876|5end_possible_flagellar_motor_protein,_OmpA_family_1819082..1819312_POSITIVE 249 52 22 150 116 pint:PI1931|5end_alpha_glycan_phosphorylase,_maltodextrin_phosphorylase_1875456..1875686_POSITIVE 249 168 122 86 39 pint:PI1816|5end_acid_phosphatase_1754646..1754876_POSITIVE 249 130 83 210 144 pint:PI1767|5end_fructokinase,_pfkB_family_1694285..1694515_POSITIVE 249 65 24 149 95 pint:PI0853|5end_glycosyl_transferase,_group_2_family_protein_785515..785745_POSITIVE 247 156 119 106 71 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 289 110 76 118 82 pint:PI0451|3end_serine_proteases,__S51_group_425127..425277_POSITIVE 271 69 30 72 21 pint:PI0188|3end_phosphomethylpyrimidine_kinase_179670..179820_POSITIVE 268 124 81 144 102 pint:PI1390|3end_hypothetical_protein_1311329..1311479_POSITIVE 261 190 141 94 51 pint:PI0168|3end_methionyl-tRNA_synthetase_(methionine--tRNA_ligase)_163279..163429_POSITIVE 258 110 61 60 6 pint:PI1240|3end_probable_Xaa-Pro_dipeptidase_(aminopeptidase_P)_1166272..1166422_POSITIVE 255 57 24 72 25 pint:PI0345|3end_conserved_hypothetical_protein_320809..320959_POSITIVE 252 190 141 76 27 pint:PI1890|3end_cell_division_protein_FtsW_1834072..1834222_POSITIVE 252 185 142 107 70 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 251 143 103 73 30 pint:PI1875|3end_hypothetical_protein_1819106..1819256_POSITIVE 248 190 144 58 12 pint:PI1000|3end_conserved_hypothetical_protein_923672..923822_POSITIVE 247 156 119 67 32 pint:PI0170|3end_hypothetical_protein_163891..164041_POSITIVE 245 187 143 72 33 pint:PI0981|3end_outer_membrane_protein,_TonB_dependent_receptor_909390..909540_POSITIVE 243 186 144 140 91 pint:PI0280|3end_conserved_hypothetical_protein_266374..266524_POSITIVE 242 70 23 139 95 pint:PI1715|3end_conserved_hypothetical_protein;_possible_DNA-binding_protein,_histone-like_family_1632324..1632474_POSITIVE 241 190 144 149 99 pint:PI0075|3end_uridine_kinase_75390..75540_POSITIVE 237 190 142 101 50 pint:PI1529|3end_cytosine/adenosine_deaminase_1450377..1450527_POSITIVE 237 47 7 46 10 pint:PI0341|3end_conserved_hypothetical_protein_317615..317765_POSITIVE 235 47 21 35 11 pint:PI1438|3end_penicillin_tolerance_protein/steroid_biosynthesis_1357275..1357425_POSITIVE 234 159 114 51 14 pint:PI0516|3end_ferredoxin,_4Fe-4S_476108..476258_POSITIVE 232 120 83 59 17 pint:PI0333|3end_hypothetical_protein_313788..313938_POSITIVE