Origin IGS: tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtagagcctgtttctactatatctttcttccataacgaaataattatttttggtaaaagtaggtgtaataaagcatatatactctacacaaaagtgtaataatcgctcatcttactgattattttagattttataacatattcagaagataacatgtttgcta Mask Tandem Repeat Region ================================================ tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Find is-nt database================================================ Query_seq: PI1550:PI1551|PI1550:PI1551:conserved hypothetical protein:HesA/MoeB/ThiF family protein:->->:1467764..1467926 163 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1550:PI1551|PI1550:PI1551:conserved hypothetical protein:HesA/MoeB/ThiF family protein:->->:1467764..1467926 163 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1550:PI1551|PI1550:PI1551:conserved hypothetical protein:HesA/MoeB/ThiF family protein:->->:1467764..1467926 163 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Predict ORF larger than 30AA ================================================ tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1550:PI1551|PI1550:PI1551:conserved hypothetical protein:HesA/MoeB/ThiF family protein:->->:1467764..1467926 163 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Intra-Species Hit: Count: 1 Min: 1 Max: 163 Len: 163 Subject: pint_PI1550_PI1551|conserved hypothetical protein:HesA/MoeB/ThiF family protein|POSITIVE:POSITIVE|[1467764,1467926]|163 HSP 1 e-value: 1.0E-87 bit: 323.0 Len: 163 Query Start:1 Query End:163 Subject Strand: POSITIVE Subject Start: 1 Subject End: 163 tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac tagcaaacatgttatcttctgaatatgttataaaatctaaaataatcagtaagatgagcgattattacacttttgtgtagagtatatatgctttattacacctacttttaccaaaaataattatttcgttatggaagaaagatatagtagaaacaggctctac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCAAACAUGUUAUCUUCUGAAUAUGUUAUAAAAUCUAAAAUAAUCAGUAAGAUGAGCGAUUAUUACACUUUUGUGUAGAGUAUAUAUGCUUUAUUACACCUACUUUUACCAAAAAUAAUUAUUUCGUUAUGGAAGAAAGAUAUAGUAGAAACAGGCUCUAC .(((.((((((((........))))))))......((((..(((....((((...(((((..((((((((....)))))..)))....)))))..)))).....(((((.(((....((((......)))))))..))))).)))..)))).....))).... (-24.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAGAGCCUGUUUCUACUAUAUCUUUCUUCCAUAACGAAAUAAUUAUUUUUGGUAAAAGUAGGUGUAAUAAAGCAUAUAUACUCUACACAAAAGUGUAAUAAUCGCUCAUCUUACUGAUUAUUUUAGAUUUUAUAACAUAUUCAGAAGAUAACAUGUUUGCUA ((((((.....))))))(((((((..(((......(((((.......)))))....))).)))))))....((((.((((....(((((....)))))..........(((((.((((.(((.(((.......))).))).)))))))))...)))).)))). (-29.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 226 105 71 36 2 pint:PI0097|5end_aspartate_aminotransferase_93235..93465_POSITIVE 225 103 69 220 181 pint:PI0466|5end_30S_ribosomal_protein_S3_435725..435955_POSITIVE 218 159 121 196 153 pint:PI0956|5end_conserved_hypothetical_protein_886077..886307_POSITIVE 216 82 53 207 172 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 215 113 71 230 191 pint:PI2097|5end_conserved_hypothetical_protein_2050912..2051142_POSITIVE 212 91 56 192 150 pint:PI2170|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_2112238..2112468_POSITIVE 212 156 126 65 31 pint:PI0125|5end_undecaprenyl_pyrophosphate_synthase_117106..117336_POSITIVE 208 84 41 133 86 pint:PI1160|5end_possible_phosphatidylinositol-4-phosphate_5-kinase_1084756..1084986_POSITIVE 208 160 124 85 44 pint:PI0245|5end_possible_mobilization_protein_242946..243176_POSITIVE 207 76 46 84 49 pint:PI0145|5end_secDF_export_membrane_protein_136627..136857_POSITIVE 204 95 53 206 157 pint:PI1019|5end_hypothetical_protein_938228..938458_POSITIVE 204 95 53 188 139 pint:PI0289|5end_hypothetical_protein_272424..272654_POSITIVE 202 90 46 228 189 pint:PI2091|5end_DNA_processing_protein_2048019..2048249_POSITIVE 202 153 118 179 138 pint:PI1118|5end_glycosyl_transferases,_group_1_1042496..1042726_POSITIVE 199 84 45 230 181 pint:PI2064|5end_conserved_hypothetical_protein;_possible_type_I_restriction-modification_system,_S_subunit_2025076..2025306_POSITIVE 199 109 61 50 5 pint:PI0116|5end_conserved_hypothetical_protein_110213..110443_POSITIVE 197 92 53 189 150 pint:PI1298|5end_glycosyltransferase_1211184..1211414_POSITIVE 196 160 111 155 104 pint:PI1849|5end_conserved_hypothetical_protein;_possible_acyltransferase_family_protein_1794916..1795146_POSITIVE 196 82 41 193 150 pint:PI1268|5end_possible_IS1249-related_transposase_1187052..1187282_POSITIVE 195 80 35 165 111 pint:PI0792|5end_conserved_hypothetical_protein_729068..729298_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 84 41 147 106 pint:PI0068|3end_arginine_repressor_67019..67169_POSITIVE 226 94 53 151 108 pint:PI0201|3end_hypothetical_protein_195398..195548_POSITIVE 222 78 41 118 76 pint:PI0413|3end_outer_membrane_protein_384019..384169_POSITIVE 221 82 46 106 71 pint:PI0099|3end_hypothetical_protein_95777..95927_POSITIVE 218 159 121 123 80 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 215 113 71 122 83 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 212 156 126 61 27 pint:PI0124|3end_outer_membrane_protein/protective_antigen_OMA87_117182..117332_POSITIVE 208 84 41 54 7 pint:PI1159|3end_1,4-alpha-glucan_branching_enzyme,_N-terminal_region_1084757..1084907_POSITIVE 204 83 45 47 8 pint:PI1366|3end_conserved_hypothetical_protein;_possible_hydrolase,_HAD_superfamily_1286647..1286797_POSITIVE 200 100 52 53 1 pint:PI0042|3end_hypothetical_protein_43444..43594_POSITIVE 195 80 35 126 72 pint:PI0791|3end_conserved_hypothetical_protein_729109..729259_POSITIVE 191 159 131 122 91 pint:PI2149|3end_3-oxoacyl-acyl-carrier-protein_synthase_2096793..2096943_POSITIVE 191 60 16 53 12 pint:PI0753|3end_hypothetical_protein_683851..684001_POSITIVE 189 85 45 99 66 pint:PI1546|3end_mobilizable_transposon,_excision_protein_1465099..1465249_POSITIVE 188 109 61 84 24 pint:PI1658|3end_conserved_hypothetical_protein_1574736..1574886_POSITIVE 188 115 71 97 42 pint:PI0051|3end_hypothetical_protein_53388..53538_POSITIVE 183 119 71 123 73 pint:PI1603|3end_hypothetical_protein_1534005..1534155_POSITIVE 183 113 71 93 41 pint:PI0473|3end_30S_ribosomal_protein_S14_439013..439163_POSITIVE 181 91 55 149 112 pint:PI0978|3end_hypothetical_protein_905114..905264_POSITIVE 180 90 44 61 19 pint:PI1991|3end_conserved_hypothetical_protein_1946370..1946520_POSITIVE