Origin IGS: tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagacaactttttgaaatactcctgtatggggttgatgcgtggagagaaactactctcgatgatggaatagagattatcggaagatgtgataatcccgatatcgtagtctatctcccttcggagcgtgttgatttcatagcgacccacttttgagaaatgagcatcgttcttacttcgatattcggttcttcccaacaccgtattgtatctgaactggtagtaagaggagagataggtttctatctcaccgtttttggaggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattctattgttgtttgtttgtaattccttttcttgttttgcgcttcaccgcacgtttctacctgcaaaagtagggagtgtttgccatacttcaaagcacgagggaacagcagggaaattcagggaaaaataaggaaaggcggttttaattttagctttttttctttgcctcctcactccattttatcatatccgttttagccgatgcaatacgatgcggattgtagcaaataagcacctgcctttcttgtatcctttacgcacttgtcctcttgtttggggcttaaatccatgcttcccgacagtttcctgtcaaaaattcgtgagaaattcattgccaatgtgcgcaacattatgcaagcactctccgaatgcttggaactttcacattctcttgataacttcactcttggaaaccaaacccaagcatataatgaaaaagaatgcagataaaggcaaatcggagggcggaaacaatattcccaagcaaaggaagacttcctccaaaaacggtgagatagaaacctatctctcctcttactaccagttcagatacaatacggtgttgggaagaaccgaatatcgaagtaagaacgatgctcatttctcaaaagtgggtcgctatgaaatcaacacgctccgaagggagatagactacgatatcgggattatcacatcttccgataatctctattccatcatcgagagtagtttctctccacgcatcaaccccatacaggagtatttcaaaaagttgtctgcaacggatataggcagcagtaaccctgattgtggaaataaagtttccctttcactgaaagccattcccgacttggcaagttgcgttgtcgtgcgcaactccgacaaatggcta Mask Tandem Repeat Region ================================================ tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagacaactttttgaaatactcctgtatggggttgatgcgtggagagaaactactctcgatgatggaatagagattatcggaagatgtgataatcccgatatcgtagtctatctcccttcggagcgtgttgatttcatagcgacccacttttgagaaatgagcatcgttcttacttcgatattcggttcttcccaacaccgtattgtatctgaactggtagtaagaggagagataggtttctatctcaccgtttttggaggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Find is-nt database================================================ Query_seq: PI1547:PI1549|PI1547:PI1549:hypothetical protein:conserved hypothetical protein:->->:1466287..1467160 874 tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagacaactttttgaaatactcctgtatggggttgatgcgtggagagaaactactctcgatgatggaatagagattatcggaagatgtgataatcccgatatcgtagtctatctcccttcggagcgtgttgatttcatagcgacccacttttgagaaatgagcatcgttcttacttcgatattcggttcttcccaacaccgtattgtatctgaactggtagtaagaggagagataggtttctatctcaccgtttttggaggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1547:PI1549|PI1547:PI1549:hypothetical protein:conserved hypothetical protein:->->:1466287..1467160 874 tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagacaactttttgaaatactcctgtatggggttgatgcgtggagagaaactactctcgatgatggaatagagattatcggaagatgtgataatcccgatatcgtagtctatctcccttcggagcgtgttgatttcatagcgacccacttttgagaaatgagcatcgttcttacttcgatattcggttcttcccaacaccgtattgtatctgaactggtagtaagaggagagataggtttctatctcaccgtttttggaggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1547:PI1549|PI1547:PI1549:hypothetical protein:conserved hypothetical protein:->->:1466287..1467160 874 tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagacaactttttgaaatactcctgtatggggttgatgcgtggagagaaactactctcgatgatggaatagagattatcggaagatgtgataatcccgatatcgtagtctatctcccttcggagcgtgttgatttcatagcgacccacttttgagaaatgagcatcgttcttacttcgatattcggttcttcccaacaccgtattgtatctgaactggtagtaagaggagagataggtttctatctcaccgtttttggaggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 118 Max: 372 Len: 255 Subject: UniRef90_Q5LA97 Cluster: Hypothetical protein; n=1; Bacteroides fragilis NCTC 9343|Rep: Hypothetical protein - Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) HSP 1 e-value: 1.0E-12 bit: 76.3 Len: 255 Query Start:118 Query End:372 Subject Strand: null Subject Start: 45 Subject End: 130 ..................................................................................................................... S K N G E I E T Y L S S Y Y Q F R Y N T V L G R T E Y R S K N D A H - F S K V G R Y E I N T L R R E I D Y D I G I I T S S D N L Y S I I E S S F S P R I N P I Q E Y F K K L ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................... S K N E R I E R Q L N S L Y D F R F N I V K S R T E Y R A A N T S G L Y Q P V T K F A L N S F R R K L D V T A G I T T S A E N I R M I L E S D F A N K V H P I R E Y L T A L ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 6 End: 125 ..... M C R S C A R Q R N L P S R E W L S V K G K L Y F H N Q G Y C C L Y P L Q T T F ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 58 Strand: + Start: 8 End: 181 ....... M S E L R T T T Q L A K S G M A F S E R E T L F P Q S G L L L P I S V A D N F L K Y S C M G L M R G E K L L S M M E ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 76 End: 264 ........................................................................... M S T I R V T A A Y I R C R Q L F E I L L Y G V D A W R E T T L D D G I E I I G R C D N P D I V V Y L P S E R V D F I A .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 271 End: 468 .............................................................................................................................................................................................................................................................................. M S I V L T S I F G S S Q H R I V S E L V V R G E I G F Y L T V F G G S L P L L G N I V S A L R F A F I C I L F H Y M L ...................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 356 End: 487 ................................................................................................................................................................................................................................................................................................................................................................... M S P F L E E V F L C L G I L F P P S D L P L S A F F F I I C L G L V S K S E V I K R M ................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 414 End: 674 ............................................................................................................................................................................................................................................................................................................................................................................................................................. M C L Y L H S F S L Y A W V W F P R V K L S R E C E S S K H S E S A C I M L R T L A M N F S R I F D R K L S G S M D L S ........................................................................................................................................................................................................ Protein_Len: 33 Strand: + Start: 622 End: 720 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M Q E R Q V L I C Y N P H R I A S A K T D M I K W S E E A K K K S .......................................................................................................................................................... Protein_Len: 43 Strand: + Start: 698 End: 826 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M R R Q R K K A K I K T A F P Y F S L N F P A V P S C F E V W Q T L P T F A G R N V R ................................................ Protein_Len: 39 Strand: + Start: 756 End: 872 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S L L F P R A L K Y G K H S L L L Q V E T C G E A Q N K K R N Y K Q T T I E .. Protein_Len: 43 Strand: - Start: 745 End: 873 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ K E R F K G A T G E H K S T H C V S G V K A P L F T R H L A F C S F S N C V F L L L M . Protein_Len: 55 Strand: - Start: 677 End: 841 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F P Y S L I S H P P L S F F S F N F G G K R I K G Q I E R S N G R A K F Y P L C E R S K C T S V H P S A C F M ................................. Protein_Len: 60 Strand: - Start: 425 End: 610 ........................................................................................................................................................................................................................................................................................................................................................................................................................................ E K E N Y A Q T Q N G L T F N D L S H S L E L C E S L A Q M I N R V N A I F K E R I K S L F S D P L M S K L G L C S S M ........................................................................................................................................................................................................................................................................ Protein_Len: 41 Strand: - Start: 225 End: 347 ................................................................................................................................................................................................................................ R G E S R T S K M A V W K Q S I L M T R V E I N P E E W C R I T D S S T T L P S M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: - Start: 105 End: 215 ........................................................................................................ I R Q L C S K S I S R Y P T S A H L S V V R S S P I S I I P L H S L G S M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 1 End: 726 R V F K Q C S V T P F C P N L G W V L P C T R L P Y L F P L H K N A V I R M T N C R S F R I H Y F P T L L C L F F A L M .................................................................................................................................................... tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 415 End: 444 ..............................................................................................................................................................................................................................................................................................................................................................................................................................TTTGCCTTTATCTGCATTCTTTTTCATTAT.............................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 46 HP_score: -3.1 Tail_Score: -3.10796 Start: 184 End: 210 Full_Region: atagactacgatatc gggattatc acatcttcc gataatctc tattccatcatcgag .......................................................................................................................................................................................gggattatcacatcttccgataatctc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 43 HP_score: -2.1 Tail_Score: -3.11316 Start: 186 End: 208 Full_Region: agactacgatatcgg gattatc acatcttcc gataatc tctattccatcatcg .........................................................................................................................................................................................gattatcacatcttccgataatc.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -6.1 Tail_Score: -4.57073 Start: 339 End: 364 Full_Region: TGAACTGGTAGTAAG AGGAGAGATAG GTTT CTATCTCACCG TTTTTGGAGGAAGTC ..................................................................................................................................................................................................................................................................................................................................................AGGAGAGATAGGTTTCTATCTCACCG.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 72 HP_score: -9.6 Tail_Score: -3.14527 Start: 340 End: 363 Full_Region: acttcctccaaaaac ggtgagatag aaac ctatctctcc tcttactaccagttc ...................................................................................................................................................................................................................................................................................................................................................ggtgagatagaaacctatctctcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -9.6 Tail_Score: -3.33922 Start: 340 End: 363 Full_Region: GAACTGGTAGTAAGA GGAGAGATAG GTTT CTATCTCACC GTTTTTGGAGGAAGT ...................................................................................................................................................................................................................................................................................................................................................GGAGAGATAGGTTTCTATCTCACC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI1547:PI1549|PI1547:PI1549:hypothetical protein:conserved hypothetical protein:->->:1466287..1467160 874 tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt Intra-Species Hit: Count: 1 Min: 1 Max: 874 Len: 874 Subject: pint_PI1547_PI1549|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1466287,1467160]|874 HSP 1 e-value: 0.0 bit: 950.0 Len: 500 Query Start:375 Query End:874 Subject Strand: POSITIVE Subject Start: 375 Subject End: 874 ......................................................................................................................................................................................................................................................................................................................................................................................aagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagnnnnnnngctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt ......................................................................................................................................................................................................................................................................................................................................................................................aagtcttcctttgcttgggaatattgtttccgccctccgatttgcctttatctgcattctttttcattatatgcttgggtttggtttccaagagtgaagttatcaagagaatgtgaaagttccaagcattcggagagtgcttgcataatgttgcgcacattggcaatgaatttctcacgaatttttgacaggaaactgtcgggaagcatggatttaagccccaaacaagaggacaagtgcgtaaaggatacaagaaaggcaggtgcttatttgctacaatccgcatcgtattgcatcggctaaaacggatatgataaaatggagtgaggaggcaaagaaaaaaagctaaaattaaaaccgcctttccttatttttccctgaatttccctgctgttccctcgtgctttgaagtatggcaaacactccctacttttgcaggtagaaacgtgcggtgaagcgcaaaacaagaaaaggaattacaaacaaacaacaatagaatt HSP 2 e-value: 2.0E-59 bit: 232.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 117 tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcaga..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tagccatttgtcggagttgcgcacgacaacgcaacttgccaagtcgggaatggctttcagtgaaagggaaactttatttccacaatcagggttactgctgcctatatccgttgcaga..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCAUUUGUCGGAGUUGCGCACGACAACGCAACUUGCCAAGUCGGGAAUGGCUUUCAGUGAAAGGGAAACUUUAUUUCCACAAUCAGGGUUACUGCUGCCUAUAUCCGUUGCAGACAACUUUUUGAAAUACUCCUGUAUGGGGUUGAUGCGUGGAGAGAAACUACUCUCGAUGAUGGAAUAGAGAUUAUCGGAAGAUGUGAUAAUCCCGAUAUCGUAGUCUAUCUCCCUUCGGAGCGUGUUGAUUUCAUAGCGACCCACUUUUGAGAAAUGAGCAUCGUUCUUACUUCGAUAUUCGGUUCUUCCCAACACCGUAUUGUAUCUGAACUGGUAGUAAGAGGAGAGAUAGGUUUCUAUCUCACCGUUUUUGGAGGAAGUCUUCCUUUGCUUGGGAAUAUUGUUUCCGCCCUCCGAUUUGCCUUUAUCUGCAUUCUUUUUCAUUAUAUGCUUGGGUUUGGUUUCCAAGAGUGAAGUUAUCAAGAGAAUGUGAAAGUUCCAAGCAUUCGGAGAGUGCUUGCAUAAUGUUGCGCACAUUGGCAAUGAAUUUCUCACGAAUUUUUGACAGGAAACUGUCGGGAAGCAUGGAUUUAAGCCCCAAACAAGAGGACAAGUGCGUAAAGGAUACAAGAAAGGCAGGUGCUUAUUUGCUACAAUCCGCAUCGUAUUGCAUCGGCUAAAACGGAUAUGAUAAAAUGGAGUGAGGAGGCAAAGAAAAAAAGCUAAAAUUAAAACCGCCUUUCCUUAUUUUUCCCUGAAUUUCCCUGCUGUUCCCUCGUGCUUUGAAGUAUGGCAAACACUCCCUACUUUUGCAGGUAGAAACGUGCGGUGAAGCGCAAAACAAGAAAAGGAAUUACAAACAAACAACAAUAGAAUU ......(((((..((((((((........)))))))).((.....(((((((((.(((((.((((.((((((..(((((((.......(((((.((((.((........)).))))......................((((..((..((((((.(((((......)))))...((((((.....(((.(((((..(((......)))))))))))....))))))..........))))))..))..))))..))))).............(((((....(((((((.......(((((((......(((......)))....))))))).)))))))((.(((((((....))))))).))......(((((....)))))..))))))))))))..))))))..(((.((((((((((((....((((((((((..(((.(((.((((((.......)))))).))).)))....))))))))))......((((.((.(((((((((..(((((.((((((.....)))))))))).)...))))).)))).(((((((((....))))))))).)).))))...............))))).))))).))...)))......(((((((.(((((((.((((((....(((.(((..((((..((((.((.....))...))))..))))..))).)))))))))......))))..........))))))))))......)))).))))).......)))))))))..(((((((((((((.((........)).)))))).(((.((....)).)))...)))))))............))........)))))............. (-249.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUCUAUUGUUGUUUGUUUGUAAUUCCUUUUCUUGUUUUGCGCUUCACCGCACGUUUCUACCUGCAAAAGUAGGGAGUGUUUGCCAUACUUCAAAGCACGAGGGAACAGCAGGGAAAUUCAGGGAAAAAUAAGGAAAGGCGGUUUUAAUUUUAGCUUUUUUUCUUUGCCUCCUCACUCCAUUUUAUCAUAUCCGUUUUAGCCGAUGCAAUACGAUGCGGAUUGUAGCAAAUAAGCACCUGCCUUUCUUGUAUCCUUUACGCACUUGUCCUCUUGUUUGGGGCUUAAAUCCAUGCUUCCCGACAGUUUCCUGUCAAAAAUUCGUGAGAAAUUCAUUGCCAAUGUGCGCAACAUUAUGCAAGCACUCUCCGAAUGCUUGGAACUUUCACAUUCUCUUGAUAACUUCACUCUUGGAAACCAAACCCAAGCAUAUAAUGAAAAAGAAUGCAGAUAAAGGCAAAUCGGAGGGCGGAAACAAUAUUCCCAAGCAAAGGAAGACUUCCUCCAAAAACGGUGAGAUAGAAACCUAUCUCUCCUCUUACUACCAGUUCAGAUACAAUACGGUGUUGGGAAGAACCGAAUAUCGAAGUAAGAACGAUGCUCAUUUCUCAAAAGUGGGUCGCUAUGAAAUCAACACGCUCCGAAGGGAGAUAGACUACGAUAUCGGGAUUAUCACAUCUUCCGAUAAUCUCUAUUCCAUCAUCGAGAGUAGUUUCUCUCCACGCAUCAACCCCAUACAGGAGUAUUUCAAAAAGUUGUCUGCAACGGAUAUAGGCAGCAGUAACCCUGAUUGUGGAAAUAAAGUUUCCCUUUCACUGAAAGCCAUUCCCGACUUGGCAAGUUGCGUUGUCGUGCGCAACUCCGACAAAUGGCUA .......(((((.((((((((((.(((.(((((......((((......))))........(((((....)))))((((((((((.(((.......(((((.((((..((((.(((.....(((((.(....((((((((((((..........((((......(((((..(....((.((((.(((..((((.((....)).))))..))).)))).))...)..))))).))))))).))))))))).).))))).........(((((.......))))).....))).))))))))(((((....))))).....(((....)))............))))).......))))))))))))).....((((((((................(((.....)))...((((...))))..)))))))).....))))).))))))))))))))))))....(((((.((((((..((((.(((.....(((..((..(.((((......((.(((((((....))))))).))(((((((....((......))....(((((((.((.....)).))))))).)))))))..(((((..............(((..((...((....))...))..)))...(((((.(((((((..((((((((..........)))))))).((((............))))))))))).)))))..)))))...........)))).)..)).....((((((((.........))))))))......))).....))).))))..)))))))))))......(((((((......((((..((((((((......))))))))))))..))))))). (-241.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 766 320 272 79 31 pint:PI0743|5end_hypothetical_protein_672728..672958_POSITIVE 487 783 741 44 2 pint:PI0740|5end_hypothetical_protein_672112..672342_POSITIVE 460 528 487 42 1 pint:PI0741|5end_conserved_hypothetical_protein_672487..672717_POSITIVE 377 61 25 47 8 pint:PI0870|5end_branched-chain_amino_acid_aminotransferase_805933..806163_POSITIVE 376 49 1 199 161 pint:PI1038|5end_hypothetical_protein_958685..958915_POSITIVE 376 50 5 72 16 pint:PI1032|5end_methylmalonyl-CoA_decarboxylase,_alpha_subunit_948463..948693_POSITIVE 364 55 12 219 172 pint:PI2005|5end_potassium_uptake_protein_1962950..1963180_POSITIVE 346 54 12 56 16 pint:PI1617|5end_hypothetical_protein_1542923..1543153_POSITIVE 345 313 275 61 22 pint:PI1779|5end_16S_rRNA_processing_protein_1711335..1711565_POSITIVE 345 438 391 190 142 pint:PI0339|5end_hypothetical_protein_315735..315965_POSITIVE 342 117 84 36 4 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 339 683 644 199 154 pint:PI0656|5end_peptidase_T_(tripeptide_aminopeptidase)_599833..600063_POSITIVE 337 676 632 40 2 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 336 810 762 231 180 pint:PI1772|5end_pullulanase_1702257..1702487_POSITIVE 335 680 633 212 162 pint:PI0634|5end_conserved_hypothetical_protein_580082..580312_POSITIVE 335 810 762 226 171 pint:PI0338|5end_hypothetical_protein_315337..315567_POSITIVE 334 796 760 230 186 pint:PI0197|5end_acetate_kinase_188602..188832_POSITIVE 334 796 760 63 19 pint:PI0196|5end_hypothetical_protein_188435..188665_POSITIVE 333 53 12 195 154 pint:PI1031|5end_hypothetical_protein_948281..948511_POSITIVE 330 849 801 142 97 pint:PI0500|5end_mobilizable_transposon,_tnpC_protein_460674..460904_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 770 174 131 151 108 pint:PI0743|3end_hypothetical_protein_673026..673176_POSITIVE 665 350 301 50 1 pint:PI0742|3end_hypothetical_protein_672749..672899_POSITIVE 369 809 762 113 51 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 354 539 493 96 48 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 350 836 797 74 39 pint:PI0042|3end_hypothetical_protein_43444..43594_POSITIVE 345 313 275 45 6 pint:PI1778|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1711399..1711549_POSITIVE 345 438 391 88 40 pint:PI0338|3end_hypothetical_protein_315713..315863_POSITIVE 335 680 633 130 80 pint:PI0633|3end_possible_preprotein_translocase,_YajC_subunit_580080..580230_POSITIVE 330 54 13 149 104 pint:PI0732|3end_conserved_hypothetical_protein_664367..664517_POSITIVE 329 258 220 126 83 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 325 48 3 64 17 pint:PI2158|3end_glycosyl_transferase,_group_2_family_protein_2105347..2105497_POSITIVE 324 56 13 111 78 pint:PI0115|3end_hypothetical_protein_108669..108819_POSITIVE 323 60 13 131 69 pint:PI0140|3end_conserved_hypothetical_protein_132106..132256_POSITIVE 321 53 11 151 98 pint:PI0258|3end_conjugative_transposon_protein,_TraG_252285..252435_POSITIVE 319 439 399 85 41 pint:PI0358|3end_hypothetical_protein_328105..328255_POSITIVE 317 56 16 148 104 pint:PI0582|3end_hypothetical_protein_525214..525364_POSITIVE 316 55 13 144 96 pint:PI1560|3end_integrase_1478265..1478415_POSITIVE 314 60 11 118 71 pint:PI2137|3end_RNA_methylase_SpoU_family_2083807..2083957_POSITIVE 314 658 618 103 67 pint:PI0545|3end_sugar_kinase_497867..498017_POSITIVE 313 48 3 70 31 pint:PI0654|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_597223..597373_POSITIVE