Origin IGS: aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcgggcaaatcaacaacagaaacaaaggcattaagcggtaacgcttaatgccttttgttgttttgcttcagtgcaactactccgtaccttttgtataaggtgtattgcggaactgtaaggaatgggtgtggactacttgattcacagacgaaaacagggtaataaatctgtaaatatttttcacaagagtaactattgtttaacttgtttattatcaacgtattacaaaacctattgttttgcgttccaaaagcgtaggtt Mask Tandem Repeat Region ================================================ aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Find is-nt database================================================ Query_seq: PI1521:PI1522|PI1521:PI1522:hypothetical protein:thioredoxin M:->->:1441522..1441785 264 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1521:PI1522|PI1521:PI1522:hypothetical protein:thioredoxin M:->->:1441522..1441785 264 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1521:PI1522|PI1521:PI1522:hypothetical protein:thioredoxin M:->->:1441522..1441785 264 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 66 End: 182 ................................................................. M V T L V K N I Y R F I T L F S S V N Q V V H T H S L Q F R N T P Y T K G T E .................................................................................. Protein_Len: 57 Strand: + Start: 44 End: 214 ........................................... M I I N K L N N S Y S C E K Y L Q I Y Y P V F V C E S S S P H P F L T V P Q Y T L Y K R Y G V V A L K Q N N K R H .................................................. Protein_Len: 30 Strand: + Start: 139 End: 228 .......................................................................................................................................... M P Y S S A I H L I Q K V R S S C T E A K Q Q K A L S V T A .................................... Protein_Len: 35 Strand: - Start: 103 End: 207 ...................................................................................................... G T K T Q S D L L G C G N R V T G C Y V K Y L L Y P T T A S F C F L M ......................................................... Protein_Len: 46 Strand: - Start: 3 End: 140 .. R R K Q F A F C Y T K Y Y T S L L C T L C Y N S K H F I N V S K N G Q K R R H I L Y D V G M ............................................................................................................................ aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -16.3 Tail_Score: -4.72277 Start: 210 End: 235 Full_Region: AGCAAAACAACAAAA GGCATTAAGCG TTAC CGCTTAATGCC TTTGTTTCTGTTGTT .................................................................................................................................................................................................................GGCATTAAGCGTTACCGCTTAATGCC............................. Find igs database================================================ Query_seq: PI1521:PI1522|PI1521:PI1522:hypothetical protein:thioredoxin M:->->:1441522..1441785 264 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Intra-Species Hit: Count: 5 Min: 1 Max: 264 Len: 264 Subject: pint_PI1521_PI1522|hypothetical protein:thioredoxin M|POSITIVE:POSITIVE|[1441522,1441785]|264 HSP 1 e-value: 1.0E-148 bit: 523.0 Len: 264 Query Start:1 Query End:264 Subject Strand: POSITIVE Subject Start: 1 Subject End: 264 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaatcaagtagtccacacccattccttacagttccgcaatacaccttatacaaaaggtacggagtagttgcactgaagcaaaacaacaaaaggcattaagcgttaccgcttaatgcctttgtttctgttgttgatttgcccgatta Subject: pint_PI2172_PI2174|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2114052,2114384]|333 HSP 1 e-value: 3.0E-18 bit: 93.7 Len: 115 Query Start:7 Query End:121 Subject Strand: NEGATIVE Subject Start: 9 Subject End: 123 ......cgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttattaccctgttttcgtctgtgaa............................................................................................................................................... ......cgcttttggaacgcaaaacaataggttttgtaatacgttgatgattagatagttaagcaatagatatatgtgcgaaaaatatttacacatttattactttcttttcgtctatgaa............................................................................................................................................... Subject: pint_PI1090_PI1091|tRNA uracil 5-methyltransferase:transcriptional regulator, AsnC family|POSITIVE:POSITIVE|[1015841,1016319]|479 HSP 1 e-value: 4.0E-17 bit: 89.7 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 207 Subject End: 307 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttatt................................................................................................................................................................... aacctacgcttttggaatgcaaaacaataggttttataacacactgatgatgaggtagttatacgataggtactcttgtgaaaaatatttacagctttatt................................................................................................................................................................... Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 4.0E-14 bit: 79.8 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 837 Subject End: 936 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttat.................................................................................................................................................................... aacctacgcttttggaatgcaaaacaataggttttataatatgttgataaacagaaagttatgcgatagttgcatctgtgaaaaatatttacagttttat.................................................................................................................................................................... Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 2.0E-10 bit: 67.9 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 240 Subject End: 341 aacctacgcttttggaacgcaaaacaataggttttgtaatacgttgataataaacaagttaaacaatagttactcttgtgaaaaatatttacagatttatta.................................................................................................................................................................. aacctacgcttttggaatgcaaaacaataggttttgcaaagtcttgatagtgagatagttatgcaatggttacgcttgtgaaaaatatttacacttttatta.................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCUACGCUUUUGGAACGCAAAACAAUAGGUUUUGUAAUACGUUGAUAAUAAACAAGUUAAACAAUAGUUACUCUUGUGAAAAAUAUUUACAGAUUUAUUACCCUGUUUUCGUCUGUGAAUCAAGUAGUCCACACCCAUUCCUUACAGUUCCGCAAUACACCUUAUACAAAAGGUACGGAGUAGUUGCACUGAAGCAAAACAACAAAAGGCAUUAAGCGUUACCGCUUAAUGCCUUUGUUUCUGUUGUUGAUUUGCCCGAUUA ...........((((...(((((((((((((((((((.....((.(((.....(((((...(((....)))...))))).......((((((((((..((......))....))))))))))......))).))............((((...(((((((.((.(((.......))).)).)).)))))))))..)))))))....((((((((((((((....))))))))))))))....)))))))...)))))))))... (-64.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGGGCAAAUCAACAACAGAAACAAAGGCAUUAAGCGGUAACGCUUAAUGCCUUUUGUUGUUUUGCUUCAGUGCAACUACUCCGUACCUUUUGUAUAAGGUGUAUUGCGGAACUGUAAGGAAUGGGUGUGGACUACUUGAUUCACAGACGAAAACAGGGUAAUAAAUCUGUAAAUAUUUUUCACAAGAGUAACUAUUGUUUAACUUGUUUAUUAUCAACGUAUUACAAAACCUAUUGUUUUGCGUUCCAAAAGCGUAGGUU .....(((((((.((((((........(((((((((((((....))))))))))))))))))).)))))))........((((....((((((......))))))......(((((.(((((((((((((((((((.....).)))))..(((...(((((........)))))...........(((((...(((....)))...)))))..........)))........))))))).)))))))))))......))))... (-73.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 733 50 1 97 48 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 703 50 1 86 37 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 651 46 1 82 37 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 633 50 1 114 65 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 629 46 1 137 92 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 628 49 1 85 37 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 621 49 1 74 26 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 619 49 7 74 32 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 606 50 1 163 114 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 606 50 1 75 26 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 601 50 1 163 114 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 578 46 1 137 92 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 569 48 7 43 2 pint:PI1922|5end_hypothetical_protein_1865219..1865449_POSITIVE 566 50 1 71 22 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 558 48 1 112 65 pint:PI1601|5end_hypothetical_protein_1530282..1530512_POSITIVE 557 50 1 86 37 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 552 48 1 84 37 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 549 46 1 82 37 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 546 40 1 230 191 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 546 49 7 113 71 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 680 50 7 49 6 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 662 50 1 63 14 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 609 47 2 46 1 pint:PI1391|3end_hypothetical_protein_1311694..1311844_POSITIVE 606 50 1 97 48 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 591 50 1 69 20 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 587 48 1 67 20 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 581 45 1 151 107 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 517 50 7 65 22 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 515 45 1 151 107 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 426 50 9 42 1 pint:PI2143|3end_DNA_mismatch_repair_protein_2091974..2092124_POSITIVE 414 46 1 93 48 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 413 60 19 49 8 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 381 120 77 81 38 pint:PI2172|3end_hypothetical_protein_2113991..2114141_POSITIVE 381 120 77 54 11 pint:PI2173|3end_hypothetical_protein_2113964..2114114_POSITIVE 370 90 42 67 19 pint:PI1600|3end_conserved_hypothetical_protein_1530275..1530425_POSITIVE 362 90 41 58 9 pint:PI0051|3end_hypothetical_protein_53388..53538_POSITIVE 361 93 53 60 20 pint:PI1117|3end_glycosyl_transferase,_family_2_1042279..1042429_POSITIVE 329 259 211 51 4 pint:PI1531|3end_biotin--acetyl-CoA-carboxylase_ligase_1451734..1451884_POSITIVE 320 61 22 40 1 pint:PI1566|3end_predicted_TPR-repeat-containing_protein_1489224..1489374_POSITIVE 315 118 75 62 20 pint:PI0770|3end_hypothetical_protein_706354..706504_POSITIVE