Origin IGS: tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaattaacaattcgggagctgatttaaggtttgtttgcaaataaagaatatgcgctaaaggagccgtttggtttcgtcttgttcagtcggtaaggttgtgtctttctgcttggaaagcgaaggggcaatatcggaatttttgttacgtgtgtaaataaaatataaattcttttgccattaccttattttacagtatctttgcatcaactttggtgtattatccccaaatttataacagatggcgaaaagacataaaaagattgagaagcctcacagcttgcaagtagtaacgctctgcataagcacggcaatggtgcttattcttatcgga Mask Tandem Repeat Region ================================================ tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Find is-nt database================================================ Query_seq: PI1510:PI1512|PI1510:PI1512:cell division protein:hypothetical protein:->->:1429335..1429668 334 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1510:PI1512|PI1510:PI1512:cell division protein:hypothetical protein:->->:1429335..1429668 334 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1510:PI1512|PI1510:PI1512:cell division protein:hypothetical protein:->->:1429335..1429668 334 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 21 End: 125 .................... M P C L C R A L L L A S C E A S Q S F Y V F S P S V I N L G I I H Q S ................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 176 End: 274 ............................................................................................................................................................................... M Y T R N K N S D I A P S L S K Q K D T T L P T E Q D E T K R L L ............................................................ Protein_Len: 47 Strand: + Start: 165 End: 305 .................................................................................................................................................................... M Y I L F T H V T K I P I L P L R F P S R K T Q P Y R L N K T K P N G S F S A Y S L F A N K P ............................. Protein_Len: 36 Strand: - Start: 177 End: 284 ................................................................................................................................................................................ K C V Y C F N R Y Q G K A K W A S L C L R V S Q V L R F W V A G K A C M .................................................. Protein_Len: 41 Strand: - Start: 48 End: 170 ............................................... K C A T L S R L R K I D K R W R N Y I Q P Y Y V L T S A F I S Y F L T I A F S N M .................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 1 End: 93 G I L I L V M A T S I C L T V V Q L S H P K E I K K H R K A M ................................................................................................................................................................................................................................................. tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1510:PI1512|PI1510:PI1512:cell division protein:hypothetical protein:->->:1429335..1429668 334 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Intra-Species Hit: Count: 1 Min: 1 Max: 334 Len: 334 Subject: pint_PI1510_PI1512|cell division protein:hypothetical protein|POSITIVE:POSITIVE|[1429335,1429668]|334 HSP 1 e-value: 0.0 bit: 662.0 Len: 334 Query Start:1 Query End:334 Subject Strand: POSITIVE Subject Start: 1 Subject End: 334 tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca tccgataagaataagcaccattgccgtgcttatgcagagcgttactacttgcaagctgtgaggcttctcaatctttttatgtcttttcgccatctgttataaatttggggataatacaccaaagttgatgcaaagatactgtaaaataaggtaatggcaaaagaatttatattttatttacacacgtaacaaaaattccgatattgccccttcgctttccaagcagaaagacacaaccttaccgactgaacaagacgaaaccaaacggctcctttagcgcatattctttatttgcaaacaaaccttaaatcagctcccgaattgttaattttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCGAUAAGAAUAAGCACCAUUGCCGUGCUUAUGCAGAGCGUUACUACUUGCAAGCUGUGAGGCUUCUCAAUCUUUUUAUGUCUUUUCGCCAUCUGUUAUAAAUUUGGGGAUAAUACACCAAAGUUGAUGCAAAGAUACUGUAAAAUAAGGUAAUGGCAAAAGAAUUUAUAUUUUAUUUACACACGUAACAAAAAUUCCGAUAUUGCCCCUUCGCUUUCCAAGCAGAAAGACACAACCUUACCGACUGAACAAGACGAAACCAAACGGCUCCUUUAGCGCAUAUUCUUUAUUUGCAAACAAACCUUAAAUCAGCUCCCGAAUUGUUAAUUUUCA ........(.((((((((.......)))))))).).(((.(((((...(((..((((((((((....((((.((((...(((.((.((.(((.............))).)).)).)))..))))))))((((((......(((...((((((...(((((....(((...(((((..((((....))))..)))))...))).))))).....((((((......))))).)...)))))).((.(((((..((.((........)).))..)))))))..)))......))))))......)))))...)))))..)))...).)))).))). (-60.02) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAUUAACAAUUCGGGAGCUGAUUUAAGGUUUGUUUGCAAAUAAAGAAUAUGCGCUAAAGGAGCCGUUUGGUUUCGUCUUGUUCAGUCGGUAAGGUUGUGUCUUUCUGCUUGGAAAGCGAAGGGGCAAUAUCGGAAUUUUUGUUACGUGUGUAAAUAAAAUAUAAAUUCUUUUGCCAUUACCUUAUUUUACAGUAUCUUUGCAUCAACUUUGGUGUAUUAUCCCCAAAUUUAUAACAGAUGGCGAAAAGACAUAAAAAGAUUGAGAAGCCUCACAGCUUGCAAGUAGUAACGCUCUGCAUAAGCACGGCAAUGGUGCUUAUUCUUAUCGGA ........(((((..(((.(((..(.....(((((.(((((..(((.....)))..)).))).)))))..)..))))))..)))))...(((((((((..((((....(((((((..((((...(((((....((((..(((((((....(((((.......)))))...((((((((((((...((((.............(((((((....)))))))..............))))..)))))).)))))).)))))))..))))...)))))...)))).)))))))..)))).....((((((((.......))))))))))))))))). (-83.43) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 354 245 205 167 125 pint:PI1223|5end_conserved_hypothetical_protein_1143537..1143767_POSITIVE 351 59 15 195 145 pint:PI1644|5end_hypothetical_protein_1560372..1560602_POSITIVE 336 65 24 128 88 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 334 68 22 210 163 pint:PI1499|5end_hypothetical_protein_1419960..1420190_POSITIVE 334 60 15 77 24 pint:PI0932|5end_conserved_hypothetical_protein;_possible_hemin_receptor_863854..864084_POSITIVE 329 280 233 208 161 pint:PI2073|5end_mobilization_protein_2031995..2032225_POSITIVE 322 68 22 76 12 pint:PI1633|5end_hypothetical_protein_1552504..1552734_POSITIVE 314 58 15 178 128 pint:PI0854|5end_MiaB-like_tRNA_modifying_enzyme_786513..786743_POSITIVE 313 64 25 75 29 pint:PI1932|5end_glycogen_synthase_1878080..1878310_POSITIVE 312 65 32 186 156 pint:PI1303|5end_ISPg3-related_transposase_1215135..1215365_POSITIVE 311 56 17 82 40 pint:PI1909|5end_DNA_gyrase,_B_subunit_1853223..1853453_POSITIVE 309 58 17 42 1 pint:PI1860|5end_hypothetical_protein_1805949..1806179_POSITIVE 309 60 14 65 7 pint:PI1475|5end_possible_short_chain_dehydrogenase_1397142..1397372_POSITIVE 309 65 21 196 153 pint:PI0956|5end_conserved_hypothetical_protein_886077..886307_POSITIVE 308 66 21 192 136 pint:PI0723|5end_conserved_hypothetical_protein_656043..656273_POSITIVE 305 69 25 58 15 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 303 304 266 223 185 pint:PI0026|5end_two-component_system_response_regulator_29756..29986_POSITIVE 301 70 22 90 26 pint:PI1461|5end_conserved_hypothetical_protein_1384364..1384594_POSITIVE 301 70 23 75 15 pint:PI0473|5end_30S_ribosomal_protein_S14_438634..438864_POSITIVE 300 69 22 169 125 pint:PI1263|5end_probable_integrase_1184502..1184732_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 390 65 22 141 94 pint:PI1347|3end_DNA_damage-inducible_protein_1262178..1262328_POSITIVE 388 65 21 64 14 pint:PI0764|3end_permease_699145..699295_POSITIVE 329 68 24 64 11 pint:PI0948|3end_hypothetical_protein_881978..882128_POSITIVE 312 65 32 138 108 pint:PI1302|3end_hypothetical_protein_1215167..1215317_POSITIVE 309 58 17 127 86 pint:PI1861|3end_hypothetical_protein_1806114..1806264_POSITIVE 309 65 21 123 80 pint:PI0955|3end_conserved_hypothetical_protein_886084..886234_POSITIVE 305 69 25 74 31 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 301 70 22 82 18 pint:PI1460|3end_ribonucleotide_reductase,_alpha_subunit,_N-terminal_region_1384436..1384586_POSITIVE 301 70 23 66 6 pint:PI0472|3end_50S_ribosomal_protein_L5_438705..438855_POSITIVE 300 66 22 140 92 pint:PI1146|3end_translation_initiation_factor_IF3_1073503..1073653_POSITIVE 300 62 23 124 78 pint:PI0561|3end_hypothetical_protein_509136..509286_POSITIVE 296 68 21 121 78 pint:PI0674|3end_conserved_hypothetical_protein_616154..616304_POSITIVE 296 57 17 101 54 pint:PI0026|3end_two-component_system_response_regulator_30537..30687_POSITIVE 295 56 11 132 81 pint:PI1253|3end_hypothetical_protein_1175691..1175841_POSITIVE 295 69 22 149 108 pint:PI1073|3end_hemogluttinin_A-related_protein_(HagA)_993693..993843_POSITIVE 293 64 22 138 96 pint:PI0700|3end_UDP-glucose_4-epimerase_638413..638563_POSITIVE 292 258 211 52 9 pint:PI1824|3end_ThiJ/PfpI_family_protein_1765005..1765155_POSITIVE 292 61 22 150 111 pint:PI1498|3end_probable_membrane_associated_lipoprotein_1419988..1420138_POSITIVE 290 60 18 62 18 pint:PI2158|3end_glycosyl_transferase,_group_2_family_protein_2105347..2105497_POSITIVE 289 66 21 98 38 pint:PI0329|3end_hypothetical_protein_310514..310664_POSITIVE