Origin IGS: aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacactttctttattatccctgcctgttcgattatagagctgaaaaagccagcctataatataataataaggtgaaaaggtcgttttataagtaaaataatacatactt Mask Tandem Repeat Region ================================================ aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Find is-nt database================================================ Query_seq: PI1500:PI1501|PI1500:PI1501:hypothetical protein:GTP-binding elongation factor family protein TypA/BipA:->->:1421453..1421563 111 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1500:PI1501|PI1500:PI1501:hypothetical protein:GTP-binding elongation factor family protein TypA/BipA:->->:1421453..1421563 111 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1500:PI1501|PI1500:PI1501:hypothetical protein:GTP-binding elongation factor family protein TypA/BipA:->->:1421453..1421563 111 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 5 End: 109 .... M Y Y F T Y K T T F S P Y Y Y I I G W L F Q L Y N R T G R D N K E S V .. aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 45 HP_score: -3.6 Tail_Score: -2.82834 Start: 51 End: 78 Full_Region: tccctgcctgttcga ttatagagctg aaaaagc cagcctataa tataataataaggtg ..................................................ttatagagctgaaaaagccagcctataa................................. Find igs database================================================ Query_seq: PI1500:PI1501|PI1500:PI1501:hypothetical protein:GTP-binding elongation factor family protein TypA/BipA:->->:1421453..1421563 111 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: pint_PI1500_PI1501|hypothetical protein:GTP-binding elongation factor family protein TypA/BipA|POSITIVE:POSITIVE|[1421453,1421563]|111 HSP 1 e-value: 7.0E-57 bit: 220.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta aagtatgtattattttacttataaaacgaccttttcaccttattattatattataggctggctttttcagctctataatcgaacaggcagggataataaagaaagtgttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUAUGUAUUAUUUUACUUAUAAAACGACCUUUUCACCUUAUUAUUAUAUUAUAGGCUGGCUUUUUCAGCUCUAUAAUCGAACAGGCAGGGAUAAUAAAGAAAGUGUUUA (((((..........))))).......(((.(((((...((((((((..((((((((((((.....))))).)))))))............)))))))).))))).))).. (-17.74) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACACUUUCUUUAUUAUCCCUGCCUGUUCGAUUAUAGAGCUGAAAAAGCCAGCCUAUAAUAUAAUAAUAAGGUGAAAAGGUCGUUUUAUAAGUAAAAUAAUACAUACUU ((((.((..(((((....(((((...((((..(((((((.((((.......)))))))))))..))))...))).)))))))..))))))...(((......)))...... (-19.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 93 56 187 150 pint:PI1543|5end_hypothetical_protein_1462773..1463003_POSITIVE 288 94 57 202 158 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 272 91 56 30 2 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 271 93 57 97 64 pint:PI1384|5end_conserved_hypothetical_protein_1305171..1305401_POSITIVE 263 68 27 230 194 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 263 68 27 147 111 pint:PI0866|5end_hypothetical_protein_803776..804006_POSITIVE 258 92 56 57 16 pint:PI1650|5end_N-acetylmuramoyl-L-alanine_amidase_1563195..1563425_POSITIVE 258 93 51 112 57 pint:PI0654|5end_K+-dependent_Na+/Ca+_exchanger_related-protein_596283..596513_POSITIVE 258 96 54 139 87 pint:PI0592|5end_DNA_mismatch_repair_protein_540795..541025_POSITIVE 256 95 57 61 17 pint:PI0525|5end_transcriptional_regulator,_Arac_family_481332..481562_POSITIVE 255 107 61 212 169 pint:PI0394|5end_transcriptional_regulator,_AraC_family_359404..359634_POSITIVE 251 95 56 60 23 pint:PI0843|5end_carboxyl-terminal_protease_776145..776375_POSITIVE 250 91 56 65 33 pint:PI1859|5end_conserved_hypothetical_protein_1805394..1805624_POSITIVE 250 92 52 150 116 pint:PI0916|5end_hypothetical_protein_845617..845847_POSITIVE 242 89 43 57 10 pint:PI0571|5end_conserved_hypothetical_protein;_possible_permease_515161..515391_POSITIVE 240 95 56 47 1 pint:PI0390|5end_conserved_hypothetical_protein_357316..357546_POSITIVE 237 106 61 150 99 pint:PI1039|5end_conserved_hypothetical_protein_957721..957951_POSITIVE 237 110 63 48 9 pint:PI0481|5end_translation_initiation_factor_IF-1_443114..443344_POSITIVE 237 92 56 213 172 pint:PI0355|5end_probable__N-acetylmuramoyl-L-alanine_amidase_326157..326387_POSITIVE 235 95 55 49 1 pint:PI1949|5end_aminotransferase,_class_V_1903218..1903448_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 239 109 70 117 74 pint:PI0102|3end_hypothetical_protein_98064..98214_POSITIVE 237 106 61 98 47 pint:PI1037|3end_probable_methyltransferase_957749..957899_POSITIVE 234 90 51 71 40 pint:PI0057|3end_conserved_hypothetical_protein_56512..56662_POSITIVE 233 95 69 44 20 pint:PI0832|3end_sigma-54-dependent_transcriptional_regulator_770155..770305_POSITIVE 229 95 55 55 10 pint:PI0267|3end_conjugative_transposon_protein,_TraQ_259291..259441_POSITIVE 228 103 61 53 6 pint:PI2112|3end_phosphopantetheine_adenylyltransferase_2063741..2063891_POSITIVE 226 85 47 130 96 pint:PI1708|3end_hypothetical_protein_1625562..1625712_POSITIVE 225 93 54 70 28 pint:PI1653|3end_hypothetical_protein_1565714..1565864_POSITIVE 223 93 52 149 100 pint:PI0409|3end_3-deoxy-D-manno-octulosonic-acid_transferase_(kdotransferase)_375969..376119_POSITIVE 221 94 51 55 19 pint:PI2113|3end_hypothetical_protein_2064278..2064428_POSITIVE 221 88 50 149 109 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 219 92 53 146 104 pint:PI0614|3end_glucose-6-phosphate_isomerase_564593..564743_POSITIVE 216 108 68 140 100 pint:PI0860|3end_amidophosphoribosyltransferase_796276..796426_POSITIVE 215 93 56 149 114 pint:PI1635|3end_hypothetical_protein_1554306..1554456_POSITIVE 215 105 61 100 57 pint:PI1153|3end_histidine_kinase_sensor_protein_1078638..1078788_POSITIVE 214 71 32 121 77 pint:PI0657|3end_conserved_hypothetical_protein_602167..602317_POSITIVE 212 59 17 139 101 pint:PI2028|3end_shikimate_kinase_1982802..1982952_POSITIVE 209 85 48 78 32 pint:PI0133|3end_hypothetical_protein_124632..124782_POSITIVE 206 72 37 134 85 pint:PI0888|3end_hypothetical_protein_818613..818763_POSITIVE 206 99 55 54 10 pint:PI0828|3end_GMP_synthase_(glutamine_amidotransferase)_765695..765845_POSITIVE