Origin IGS: taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttccaccttttttgtttggtcaataacgtgttcttactgaattattcttcaaatgtacgaataattttgggaatatgccgttgccttaaagtatattttataaaatgaagtttcctataaaaagtta Mask Tandem Repeat Region ================================================ taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Find is-nt database================================================ Query_seq: PI1472:PI1474|PI1472:PI1474:hypothetical protein:conserved hypothetical protein:->->:1395940..1396066 127 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1472:PI1474|PI1472:PI1474:hypothetical protein:conserved hypothetical protein:->->:1395940..1396066 127 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1472:PI1474|PI1472:PI1474:hypothetical protein:conserved hypothetical protein:->->:1395940..1396066 127 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 23 End: 112 ...................... K I F Y V K L C R C I G L I I R V N S S Y N L L F V N N V M ............... taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1472:PI1474|PI1472:PI1474:hypothetical protein:conserved hypothetical protein:->->:1395940..1396066 127 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: pint_PI1472_PI1474|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1395940,1396066]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa taactttttataggaaacttcattttataaaatatactttaaggcaacggcatattcccaaaattattcgtacatttgaagaataattcagtaagaacacgttattgaccaaacaaaaaaggtggaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUUUUUAUAGGAAACUUCAUUUUAUAAAAUAUACUUUAAGGCAACGGCAUAUUCCCAAAAUUAUUCGUACAUUUGAAGAAUAAUUCAGUAAGAACACGUUAUUGACCAAACAAAAAAGGUGGAA ..(((((((..((....)).......................(((((..((...(((....((((((((...........))))))))......)))...))..))).))......))))))).... (-16.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCCACCUUUUUUGUUUGGUCAAUAACGUGUUCUUACUGAAUUAUUCUUCAAAUGUACGAAUAAUUUUGGGAAUAUGCCGUUGCCUUAAAGUAUAUUUUAUAAAAUGAAGUUUCCUAUAAAAAGUUA ......((((((.....((.((((..((((((((((..(((((((((...........)))))))))))))))))))..))))))....((...(((((((...)))))))...))..))))))... (-22.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 98 54 115 71 pint:PI0717|5end_conserved_hypothetical_protein_652558..652788_POSITIVE 238 90 42 163 108 pint:PI0791|5end_conserved_hypothetical_protein_728361..728591_POSITIVE 215 74 38 172 131 pint:PI1226|5end_hypothetical_protein_1147884..1148114_POSITIVE 215 74 38 46 5 pint:PI1225|5end_hypothetical_protein_1147758..1147988_POSITIVE 213 80 33 79 26 pint:PI1718|5end_DNA_mismatch_repair_protein_1633997..1634227_POSITIVE 210 82 41 95 54 pint:PI1068|5end_nucleolar_protein,_Sun_(Fmu)_family_protein;_tRNA/rRNA_cytosine-C5-methylase_984743..984973_POSITIVE 203 49 13 55 25 pint:PI1488|5end_hypothetical_protein_1406994..1407224_POSITIVE 200 100 58 144 101 pint:PI0382|5end_conserved_hypothetical_protein_351510..351740_POSITIVE 197 110 69 111 68 pint:PI2178|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase,_GcpE_2117677..2117907_POSITIVE 197 111 81 42 12 pint:PI0833|5end_conserved_hypothetical_protein_770059..770289_POSITIVE 196 100 52 191 137 pint:PI1117|5end_glycosyl_transferase,_family_2_1041318..1041548_POSITIVE 196 70 36 80 54 pint:PI0964|5end_conserved_hypothetical_protein_893238..893468_POSITIVE 196 70 36 40 14 pint:PI0963|5end_hypothetical_protein_893198..893428_POSITIVE 194 56 12 163 117 pint:PI1584|5end_A/G-specific_adenine_glycosylase_1511898..1512128_POSITIVE 194 61 37 168 138 pint:PI0715|5end_hypothetical_protein_652311..652541_POSITIVE 192 73 34 93 34 pint:PI2096|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2050268..2050498_POSITIVE 192 78 48 40 14 pint:PI0906|5end_hypothetical_protein_835653..835883_POSITIVE 192 76 34 166 126 pint:PI0732|5end_conserved_hypothetical_protein_663598..663828_POSITIVE 192 71 34 222 186 pint:PI0078|5end_hypothetical_protein_77768..77998_POSITIVE 191 88 41 63 21 pint:PI2169|5end_hypothetical_protein_2111706..2111936_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 98 54 98 54 pint:PI0715|3end_hypothetical_protein_652621..652771_POSITIVE 256 98 54 82 38 pint:PI0716|3end_conserved_hypothetical_protein_652605..652755_POSITIVE 238 90 42 107 52 pint:PI0789|3end_NAD-utilizing_dehydrogenase_728385..728535_POSITIVE 215 74 38 141 100 pint:PI1225|3end_hypothetical_protein_1147933..1148083_POSITIVE 210 59 20 138 98 pint:PI1913|3end_conserved_hypothetical_protein_1858008..1858158_POSITIVE 210 82 41 83 42 pint:PI1067|3end_thymidylate_synthase_984811..984961_POSITIVE 208 90 48 83 41 pint:PI0647|3end_potassium_uptake_protein_589276..589426_POSITIVE 203 49 13 43 13 pint:PI1486|3end_alginate_O-acetylation_protein_1407062..1407212_POSITIVE 200 100 58 108 65 pint:PI0381|3end_probable_transcriptional_regulator,_AraC_family_351554..351704_POSITIVE 197 110 69 64 21 pint:PI2177|3end_phosphoribosylaminoimidazole_carboxylase_2117710..2117860_POSITIVE 197 111 81 58 28 pint:PI0832|3end_sigma-54-dependent_transcriptional_regulator_770155..770305_POSITIVE 192 110 66 80 28 pint:PI0537|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_492412..492562_POSITIVE 190 59 47 50 38 pint:PI1627|3end_hypothetical_protein_1551157..1551307_POSITIVE 186 108 61 94 47 pint:PI1055|3end_aspartokinase_976302..976452_POSITIVE 184 81 46 42 12 pint:PI2079|3end_conserved_hypothetical_protein;_possible_ATPase_2037961..2038111_POSITIVE 184 57 42 33 14 pint:PI0660|3end_conserved_hypothetical_protein_603997..604147_POSITIVE 179 58 13 117 79 pint:PI1748|3end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase/possible_(p)ppgpp_synthetase_II_1670922..1671072_POSITIVE 178 101 64 43 3 pint:PI0268|3end_lysozyme-related_protein_259790..259940_POSITIVE 176 61 41 82 55 pint:PI0017|3end_thiol-disulfide_isomerase;_thioredoxin_22835..22985_POSITIVE 173 84 41 106 66 pint:PI1818|3end_hypothetical_protein_1757270..1757420_POSITIVE