Origin IGS: attaggtccgccaacacgttgtgtaaagcgtttcacggtaacggctcctgcagtatcggcaagaaactccaattcgtctaagtattctttggtcttggcttcgtcctgctcttgcgttatcagtcctacaagtatggcagtttcggctttgacttccgatataacaaattctttcattcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgggtttctgtaaggggcagtacagagttttgaaagtgcttttgtacagctaacacaaagctacgcacacttttctgtatcaacgatattgtagtccctgttatgtccttttaggttgcaaaattacgttattcctacataaagacacgataaacaacacattatttataaaaaataacaattgcgaataaaaaacgttcaaacgcttcttatgaactcttaactttttattaactttgtagccacattatatatagtactattttttgaatgaaagaatttgttatatcggaagtcaaagccgaaactgccatacttgtaggactgataacgcaagagcaggacgaagccaagaccaaagaatacttagacgaattggagtttcttgccgatactgcaggagccgttaccgtgaaacgctttacacaacgtgttggcggacctaat Mask Tandem Repeat Region ================================================ attaggtccgccaacacgttgtgtaaagcgtttcacggtaacggctcctgcagtatcggcaagaaactccaattcgtctaagtattctttggtcttggcttcgtcctgctcttgcgttatcagtcctacaagtatggcagtttcggctttgacttccgatataacaaattctttcattcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Find is-nt database================================================ Query_seq: PI1466:PI1467|PI1466:PI1467:GTP-binding protein, possible GTP1/OBG family:conserved hypothetical protein:->->:1393194..1393642 449 attaggtccgccaacacgttgtgtaaagcgtttcacggtaacggctcctgcagtatcggcaagaaactccaattcgtctaagtattctttggtcttggcttcgtcctgctcttgcgttatcagtcctacaagtatggcagtttcggctttgacttccgatataacaaattctttcattcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1466:PI1467|PI1466:PI1467:GTP-binding protein, possible GTP1/OBG family:conserved hypothetical protein:->->:1393194..1393642 449 attaggtccgccaacacgttgtgtaaagcgtttcacggtaacggctcctgcagtatcggcaagaaactccaattcgtctaagtattctttggtcttggcttcgtcctgctcttgcgttatcagtcctacaagtatggcagtttcggctttgacttccgatataacaaattctttcattcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1466:PI1467|PI1466:PI1467:GTP-binding protein, possible GTP1/OBG family:conserved hypothetical protein:->->:1393194..1393642 449 attaggtccgccaacacgttgtgtaaagcgtttcacggtaacggctcctgcagtatcggcaagaaactccaattcgtctaagtattctttggtcttggcttcgtcctgctcttgcgttatcagtcctacaagtatggcagtttcggctttgacttccgatataacaaattctttcattcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 18 Min: 1 Max: 177 Len: 177 Subject: UniRef90_Q8A5I1 Cluster: GTP-binding protein; n=3; Bacteroides|Rep: GTP-binding protein - Bacteroides thetaiotaomicron HSP 1 e-value: 4.0E-16 bit: 85.9 Len: 177 Query Start:1 Query End:177 Subject Strand: null Subject Start: 1 Subject End: 59 M K E F V I S E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................ M K E F I I S E A K V E T A V L V G L I T Q M Q D E R K T N E Y L D E L A F L A E T A G A E V V K R F T Q K L P T A N ................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q7MTR0 Cluster: GTP-binding protein HflX; n=1; Porphyromonas gingivalis|Rep: GTP-binding protein HflX - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 1.0E-12 bit: 74.3 Len: 177 Query Start:1 Query End:177 Subject Strand: null Subject Start: 1 Subject End: 59 M K E F V I S E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................ M K E F I I S E A R T E P T V L V G L I T P Q Q S E T Q V R E Y L D E L A F L C E T A G A Q P I A R F T Q K L D Q P N ................................................................................................................................................................................................................................................................................ Subject: UniRef90_A2TYV5 Cluster: GTP-binding protein HflX; n=1; Polaribacter dokdonensis MED152|Rep: GTP-binding protein HflX - Polaribacter dokdonensis MED152 HSP 1 e-value: 5.0E-11 bit: 68.9 Len: 156 Query Start:1 Query End:156 Subject Strand: null Subject Start: 6 Subject End: 57 E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ..................................................................................................................................................................................................................................................................................................... E A I S E K A V L I G V I T Q Q Q D E T K S T E Y L D E L E F L T Q T A G G V V V K R F V Q K M E R P N ..................................................................................................................................................................................................................................................................................................... Subject: UniRef90_A4BWN5 Cluster: GTP-binding protein HflX; n=1; Polaribacter irgensii 23-P|Rep: GTP-binding protein HflX - Polaribacter irgensii 23-P HSP 1 e-value: 1.0E-10 bit: 67.4 Len: 156 Query Start:1 Query End:156 Subject Strand: null Subject Start: 6 Subject End: 57 E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ..................................................................................................................................................................................................................................................................................................... E A I S E K A V L I G V I T Q Q Q D E T Q S D E Y L D E L E F L T S T A G G V A V K R F V Q K M E K P N ..................................................................................................................................................................................................................................................................................................... Subject: UniRef90_A3XHI6 Cluster: GTP-binding protein HflX; n=1; Leeuwenhoekiella blandensis MED217|Rep: GTP-binding protein HflX - Leeuwenhoekiella blandensis MED217 HSP 1 e-value: 7.0E-10 bit: 65.1 Len: 159 Query Start:1 Query End:159 Subject Strand: null Subject Start: 5 Subject End: 57 S E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N .................................................................................................................................................................................................................................................................................................. T K I E Y E K T V L I G L I T Q F Q D E D K A T E Y L D E L E F L A Y T A G G E V L K R F T Q K M D T P N .................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A3U6X5 Cluster: GTP-binding protein HflX; n=1; Croceibacter atlanticus HTCC2559|Rep: GTP-binding protein HflX - Croceibacter atlanticus HTCC2559 HSP 1 e-value: 1.0E-9 bit: 64.3 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 10 Subject End: 57 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E S A V L I G V I T Q L Q D E E Q S K E Y L D E L E F L T Y T A G G E V L K R F T Q K L E H P N ................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A2TRS4 Cluster: GTP-binding protein HflX; n=1; Dokdonia donghaensis MED134|Rep: GTP-binding protein HflX - Dokdonia donghaensis MED134 HSP 1 e-value: 4.0E-9 bit: 62.8 Len: 162 Query Start:1 Query End:162 Subject Strand: null Subject Start: 4 Subject End: 57 I S E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ............................................................................................................................................................................................................................................................................................... V E K I E Y E K C I L V G L V T Q K Q T H E K M T E Y L D E L E F L A Y T A G A E V L K R F S Q K L E R P N ............................................................................................................................................................................................................................................................................................... Subject: UniRef90_A0M622 Cluster: HflX-like GTP-binding protein; n=1; Gramella forsetii KT0803|Rep: HflX-like GTP-binding protein - Gramella forsetii (strain KT0803) HSP 1 e-value: 5.0E-9 bit: 62.4 Len: 159 Query Start:1 Query End:159 Subject Strand: null Subject Start: 5 Subject End: 57 S E V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N .................................................................................................................................................................................................................................................................................................. T D L S Y E R T V L I G I V T R E Q D E E K L E E Y L D E L E F L T Y T A G G E V I K R F S Q R L D K P D .................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q26GV0 Cluster: GTP-binding protein, HflX; n=1; Flavobacteria bacterium BBFL7|Rep: GTP-binding protein, HflX - Flavobacteria bacterium BBFL7 HSP 1 e-value: 5.0E-9 bit: 62.4 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 10 Subject End: 57 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E K A I L V G V V T Q Q Q S E E K L T E Y L D E L E F L A Y T A G A T I H K R F S Q K M Q K P N ................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A3J318 Cluster: GTP-binding protein HflX; n=1; Flavobacteria bacterium BAL38|Rep: GTP-binding protein HflX - Flavobacteria bacterium BAL38 HSP 1 e-value: 1.0E-8 bit: 60.8 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 10 Subject End: 57 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E K S I I V G I V T Q N Q S E E K L N E Y L D E L E F L T F T A G G T V V K R F S Q K M D K P N ................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A4CHL1 Cluster: GTP-binding protein HflX; n=1; Robiginitalea biformata HTCC2501|Rep: GTP-binding protein HflX - Robiginitalea biformata HTCC2501 HSP 1 e-value: 2.0E-8 bit: 60.5 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 10 Subject End: 57 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E K A V L I G I I T R E Q P E E K V N E Y L D E L E F L T Y T A G G E V V G R F V Q R I D V P N ................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A4ANU4 Cluster: GTP-binding protein HflX; n=1; Flavobacteriales bacterium HTCC2170|Rep: GTP-binding protein HflX - Flavobacteriales bacterium HTCC2170 HSP 1 e-value: 2.0E-8 bit: 60.1 Len: 153 Query Start:1 Query End:153 Subject Strand: null Subject Start: 7 Subject End: 57 V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ........................................................................................................................................................................................................................................................................................................ I E Y E K A V L I G V I N R E Q N E E K V N E Y L D E L E F L T Y T A G G E V S K R F V Q R V E V P N ........................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q1VSY2 Cluster: GTP-binding protein HflX; n=1; Psychroflexus torquis ATCC 700755|Rep: GTP-binding protein HflX - Psychroflexus torquis ATCC 700755 HSP 1 e-value: 3.0E-8 bit: 59.7 Len: 153 Query Start:1 Query End:153 Subject Strand: null Subject Start: 7 Subject End: 57 V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ........................................................................................................................................................................................................................................................................................................ I N Y E T T V L I G I V N Q F Q N E E K S K E Y L D E L E F L T Y T A G G E V L K R F Q Q K L E V P N ........................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q1XUV6 Cluster: GTP-binding protein, HSR1-related; n=1; Flavobacterium johnsoniae UW101|Rep: GTP-binding protein, HSR1-related - Flavobacterium johnsoniae UW101 HSP 1 e-value: 2.0E-7 bit: 57.4 Len: 153 Query Start:1 Query End:153 Subject Strand: null Subject Start: 7 Subject End: 57 V K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ........................................................................................................................................................................................................................................................................................................ I N F E K T A I V G I V T Q N Q S E E K L N E Y L D E L E F L T F T A G G E V I K R F S Q K M E R P N ........................................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q4AFC7 Cluster: GTP-binding protein, HSR1-related; n=1; Chlorobium phaeobacteroides BS1|Rep: GTP-binding protein, HSR1-related - Chlorobium phaeobacteroides BS1 HSP 1 e-value: 4.0E-7 bit: 55.8 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 12 Subject End: 59 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E K A I L V G L I T R D T N K I K A N E Y L D E L S F L V E T A G G I P V A R F V Q A I D T P H ................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q11NE1 Cluster: GTP-binding protein, possible GTP1/OBG family; n=1; Cytophaga hutchinsonii ATCC 33406|Rep: GTP-binding protein, possible GTP1/OBG family - Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) HSP 1 e-value: 2.0E-6 bit: 53.5 Len: 147 Query Start:1 Query End:147 Subject Strand: null Subject Start: 14 Subject End: 62 A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N .............................................................................................................................................................................................................................................................................................................. A D K A I L I G L S H R N Q P Q E K T I E Y L D E L D F L A R T A G L E V V Y R F T Q K L D R P D .............................................................................................................................................................................................................................................................................................................. Subject: UniRef90_A3HU64 Cluster: GTP-binding protein HflX; n=1; Algoriphagus sp. PR1|Rep: GTP-binding protein HflX - Algoriphagus sp. PR1 HSP 1 e-value: 5.0E-6 bit: 52.4 Len: 150 Query Start:1 Query End:150 Subject Strand: null Subject Start: 17 Subject End: 66 K A E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ........................................................................................................................................................................................................................................................................................................... K I D T A V L V S I V R Q G Q T E M E I D E H L D E L A F L T E T L G A K T V Y R F T Q K M E K P D ........................................................................................................................................................................................................................................................................................................... Subject: UniRef90_A1ZKR7 Cluster: GTP-binding protein HflX; n=1; Microscilla marina ATCC 23134|Rep: GTP-binding protein HflX - Microscilla marina ATCC 23134 HSP 1 e-value: 6.0E-6 bit: 52.0 Len: 144 Query Start:1 Query End:144 Subject Strand: null Subject Start: 12 Subject End: 59 E T A I L V G L I T Q E Q D E A K T K E Y L D E L E F L A D T A G A V T V K R F T Q R V G G P N ................................................................................................................................................................................................................................................................................................................. E T A V L V A V I R T N Q T E E A T Q A Y L D E L E F L A K T L G V K T L A N F T Q R L E T P D ................................................................................................................................................................................................................................................................................................................. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 48 End: 149 ............................................... M Q Y R Q E T P I R L S I L W S W L R P A L A L S V L Q V W Q F R L ............................................................................................................................................................................................................................................................................................................ Protein_Len: 42 Strand: + Start: 89 End: 214 ........................................................................................ M V L A S S C S C V I S P T S M A V S A L T S D I T N S F I Q K I V L Y I M W L Q S ........................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 106 End: 225 ......................................................................................................... M L L R Y Q S Y K Y G S F G F D F R Y N K F F H S K N S T I Y N V A T K L I K S ................................................................................................................................................................................................................................ Protein_Len: 33 Strand: + Start: 246 End: 344 ..................................................................................................................................................................................................................................................... M N V F Y S Q L L F F I N N V L F I V S L C R N N V I L Q P K R T ......................................................................................................... Protein_Len: 35 Strand: + Start: 344 End: 448 ....................................................................................................................................................................................................................................................................................................................................................... M T G T T I S L I Q K S V R S F V L A V Q K H F Q N S V L P L T E T Q . Protein_Len: 38 Strand: - Start: 260 End: 373 ................................................................................................................................................................................................................................................................... E C N N N K I F L T N N I T D K H L F L T I K C G L L V Y C P S C Y R Q Y M ............................................................................ Protein_Len: 60 Strand: - Start: 5 End: 181 .... * T R W C T T Y L T E R Y R S R C Y R C S V G I R R L I R Q D Q S R G A R A N D T R C T H C N R S Q S G I Y C I R E N M ............................................................................................................................................................................................................................................................................ Protein_Len: 59 Strand: - Start: 1 End: 177 N P G G V R Q T F R K V T V A G A T D A L F E L E D L Y E K T K A E D Q E Q T I L G V L I A T E A K V E S I V F E K M ................................................................................................................................................................................................................................................................................ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 172 End: 201 ...........................................................................................................................................................................TTTCATTCAAAAAATAGTACTATATATAAT........................................................................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1466:PI1467|PI1466:PI1467:GTP-binding protein, possible GTP1/OBG family:conserved hypothetical protein:->->:1393194..1393642 449 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Intra-Species Hit: Count: 1 Min: 178 Max: 449 Len: 272 Subject: pint_PI1466_PI1467|GTP-binding protein, possible GTP1/OBG family:conserved hypothetical protein|POSITIVE:POSITIVE|[1393194,1393642]|449 HSP 1 e-value: 1.0E-152 bit: 539.0 Len: 272 Query Start:178 Query End:449 Subject Strand: POSITIVE Subject Start: 178 Subject End: 449 .................................................................................................................................................................................tcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa .................................................................................................................................................................................tcaaaaaatagtactatatataatgtggctacaaagttaataaaaagttaagagttcataagaagcgtttgaacgttttttattcgcaattgttattttttataaataatgtgttgtttatcgtgtctttatgtaggaataacgtaattttgcaacctaaaaggacataacagggactacaatatcgttgatacagaaaagtgtgcgtagctttgtgttagctgtacaaaagcactttcaaaactctgtactgccccttacagaaacccaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAAAAAAUAGUACUAUAUAUAAUGUGGCUACAAAGUUAAUAAAAAGUUAAGAGUUCAUAAGAAGCGUUUGAACGUUUUUUAUUCGCAAUUGUUAUUUUUUAUAAAUAAUGUGUUGUUUAUCGUGUCUUUAUGUAGGAAUAACGUAAUUUUGCAACCUAAAAGGACAUAACAGGGACUACAAUAUCGUUGAUACAGAAAAGUGUGCGUAGCUUUGUGUUAGCUGUACAAAAGCACUUUCAAAACUCUGUACUGCCCCUUACAGAAACCCAAA ..........(((((((((...)))))).)))..............((((...((((.((((((((((....))))))))))...((((..(((((((.....)))))))..(((((((..((((....))))...)))))))......)))).........)))).)))).(((....................((((.(((((..(((((.......)))))..)....)))))))).....((((((........))))))..)))... (-52.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGGGUUUCUGUAAGGGGCAGUACAGAGUUUUGAAAGUGCUUUUGUACAGCUAACACAAAGCUACGCACACUUUUCUGUAUCAACGAUAUUGUAGUCCCUGUUAUGUCCUUUUAGGUUGCAAAAUUACGUUAUUCCUACAUAAAGACACGAUAAACAACACAUUAUUUAUAAAAAAUAACAAUUGCGAAUAAAAAACGUUCAAACGCUUCUUAUGAACUCUUAACUUUUUAUUAACUUUGUAGCCACAUUAUAUAUAGUACUAUUUUUUGA ...((((((..(((((((((.((((((((...((...((((((((((.......))).))))).))...))...)))))))).((((.((((((.(((....((((((......(((..((........))....))))))))).))))))))).........(((((..(((..........)))..)))))....)))).....)))))))))))))))..............(((.((((.........)))).)))............ (-46.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 259 213 205 157 pint:PI1532|5end_uridylate_kinase_1451644..1451874_POSITIVE 301 256 211 109 70 pint:PI0707|5end_conserved_hypothetical_protein;_possible_integral_membrane_protein_643358..643588_POSITIVE 297 250 201 218 163 pint:PI0204|5end_dGTP_triphosphohydrolase_(deoxyguanosinetriphosphate_triphosphohydrolase)_196119..196349_POSITIVE 291 268 227 44 2 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 284 220 171 203 158 pint:PI2048|5end_possible_two-component_system_sensor_histidine_kinase_2000705..2000935_POSITIVE 284 254 212 226 188 pint:PI1565|5end_conserved_hypothetical_protein;_possible_surface_antigen_1485460..1485690_POSITIVE 282 230 186 202 149 pint:PI2109|5end_surface_antigen_BspA_2060553..2060783_POSITIVE 279 258 211 198 142 pint:PI0197|5end_acetate_kinase_188602..188832_POSITIVE 269 259 211 63 15 pint:PI1637|5end_hypothetical_protein_1554500..1554730_POSITIVE 266 217 172 207 161 pint:PI1974|5end_hypothetical_protein_1931193..1931423_POSITIVE 266 222 184 58 17 pint:PI0519|5end_5'-nucleotidase/2',3'-cyclic_phosphodiesterase_477953..478183_POSITIVE 264 222 181 192 151 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 264 222 181 104 63 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 260 219 172 64 4 pint:PI0516|5end_ferredoxin,_4Fe-4S_475864..476094_POSITIVE 260 269 222 67 10 pint:PI0263|5end_conjugative_transposon_protein,_TraM_255256..255486_POSITIVE 259 225 181 197 149 pint:PI1924|5end_cytidine_deaminase_1865897..1866127_POSITIVE 258 226 190 179 143 pint:PI1999|5end_hypothetical_protein_1956457..1956687_POSITIVE 257 217 171 68 6 pint:PI1333|5end_conserved_hypothetical_protein_1250777..1251007_POSITIVE 252 256 214 54 16 pint:PI0812|5end_conserved_hypothetical_protein;_possible_ferredoxin_750185..750415_POSITIVE 250 222 184 97 62 pint:PI1002|5end_metal_dependent_hydrolase_923842..924072_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 256 211 65 26 pint:PI0706|3end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_643394..643544_POSITIVE 297 250 201 83 28 pint:PI0203|3end_conserved_hypothetical_protein_196064..196214_POSITIVE 291 268 227 145 103 pint:PI0024|3end_hypothetical_protein_28559..28709_POSITIVE 288 208 162 68 23 pint:PI1704|3end_50S_ribosomal_protein_L32_1621680..1621830_POSITIVE 284 220 171 124 79 pint:PI2047|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino_pimelate--D-alanyl-D-alanyl_ligase_2000706..2000856_POSITIVE 266 217 172 105 59 pint:PI1973|3end_conserved_hypothetical_protein_1931171..1931321_POSITIVE 264 222 181 126 85 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 261 223 184 149 112 pint:PI0806|3end_heavy_metal_efflux_pump_744394..744544_POSITIVE 259 225 181 132 84 pint:PI1923|3end_conserved_hypothetical_protein_1865912..1866062_POSITIVE 252 234 193 70 35 pint:PI0141|3end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_133308..133458_POSITIVE 251 234 200 78 49 pint:PI0147|3end_HIT_family_protein_141308..141458_POSITIVE 250 222 184 97 62 pint:PI1001|3end_hypothetical_protein_923922..924072_POSITIVE 248 217 185 130 98 pint:PI0223|3end_topoisomerase_IA_220583..220733_POSITIVE 247 235 206 148 117 pint:PI1775|3end_hypothetical_protein_1708120..1708270_POSITIVE 246 228 203 50 19 pint:PI1837|3end_aspartate_ammonia-lyase_1780406..1780556_POSITIVE 246 238 193 101 58 pint:PI1491|3end_conserved_hypothetical_protein_1410188..1410338_POSITIVE 244 219 171 134 82 pint:PI1583|3end_phosphoribosylaminoimidazolecarboxamide_formyltransferase_(AICAR)/IMP_cyclohydrolase_(ATIC)_1511200..1511350_POSITIVE 243 228 182 140 80 pint:PI1662|3end_hypothetical_protein_1577241..1577391_POSITIVE 242 249 205 103 55 pint:PI0118|3end_hypothetical_protein_111640..111790_POSITIVE 241 259 214 147 96 pint:PI1261|3end_ISPg3-related_transposase,_N-terminal_region_1184074..1184224_POSITIVE