Origin IGS: tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaaggagcatctgaaagcaatgcaaggtcttgaattatcacgttcctgccacttacgaaaagcaagatggaatatgcggaatgtagtaaattgcacccttgcatttcaccttgagaaagcaaagaaataaccctggttttgtaaagataatttacaaggaaaacggtattttcgatttgacgttgcgaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggttttgtaaagcgttgataacgagtttgttacacgagagatgagtttgtaaaaatctttacatatttatcacctgtttttagcttgtcta Mask Tandem Repeat Region ================================================ tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Find is-nt database================================================ Query_seq: PI1441:PI1442|PI1441:PI1442:hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme):->->:1363734..1364088 355 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1441:PI1442|PI1441:PI1442:hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme):->->:1363734..1364088 355 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1441:PI1442|PI1441:PI1442:hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme):->->:1363734..1364088 355 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 26 End: 244 ......................... M C K D F Y K L I S R V T N S L S T L Y K T Y C F A F Q K R R F C T V K A A L L H C K T Y A F A T S N R K Y R F P C K L ............................................................................................................... Protein_Len: 32 Strand: + Start: 202 End: 297 ......................................................................................................................................................................................................... M I F T K P G L F L C F L K V K C K G A I Y Y I P H I P S C F S .......................................................... Protein_Len: 60 Strand: + Start: 147 End: 353 .................................................................................................................................................. M A K P T L S Q R Q I E N T V F L V N Y L Y K T R V I S L L S Q G E M Q G C N L L H S A Y S I L L F V S G R N V I I Q D .. Protein_Len: 33 Strand: + Start: 257 End: 355 ................................................................................................................................................................................................................................................................ M Q F T T F R I F H L A F R K W Q E R D N S R P C I A F R C S F S Protein_Len: 39 Strand: - Start: 226 End: 342 ................................................................................................................................................................................................................................. K K A K E L H F A L T C N V V N R M N W R A K R L H C S R S L E L G Q M A K M ............. Protein_Len: 60 Strand: - Start: 51 End: 263 .................................................. C F R N N Q T G F A Y T K C P L L P E K A N C F R R K R L T L D F I G N E K Y I I K V F G P N N R Q K R L T F H L P A M ............................................................................................ tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -19.3 Tail_Score: -3.54424 Start: 112 End: 162 Full_Region: TTTTGCGTTCCAAAA GCGTAGGTTTTGCACGGTAAAAG CGGCT CTTTTGCATTGCAAAACCTACGC TTTCGCAACGTCAAA ...............................................................................................................GCGTAGGTTTTGCACGGTAAAAGCGGCTCTTTTGCATTGCAAAACCTACGC................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -17.1 Tail_Score: -4.07568 Start: 112 End: 162 Full_Region: tttgacgttgcgaaa gcgtaggttttgcaatgcaaaag agccg cttttaccgtgcaaaacctacgc ttttggaacgcaaaa ...............................................................................................................gcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgc................................................................................................................................................................................................. Find igs database================================================ Query_seq: PI1441:PI1442|PI1441:PI1442:hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme):->->:1363734..1364088 355 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Intra-Species Hit: Count: 32 Min: 1 Max: 355 Len: 355 Subject: pint_PI1441_PI1442|hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme)|POSITIVE:POSITIVE|[1363734,1364088]|355 HSP 1 e-value: 0.0 bit: 704.0 Len: 355 Query Start:1 Query End:355 Subject Strand: POSITIVE Subject Start: 1 Subject End: 355 tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca tagacaagctaaaaacaggtgataaatatgtaaagatttttacaaactcatctctcgtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaaccagggttatttctttgctttctcaaggtgaaatgcaagggtgcaatttactacattccgcatattccatcttgcttttcgtaagtggcaggaacgtgataattcaagaccttgcattgctttcagatgctccttttca Subject: pint_PI0831_PI0832|hypothetical protein:sigma-54-dependent transcriptional regulator|POSITIVE:POSITIVE|[768509,768973]|465 HSP 1 e-value: 9.0E-47 bit: 188.0 Len: 107 Query Start:71 Query End:177 Subject Strand: POSITIVE Subject Start: 164 Subject End: 270 ......................................................................ttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ......................................................................ttatcaacgctttgtaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctgttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. Subject: pint_PI1391_PI1392|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1311755,1312040]|286 HSP 1 e-value: 4.0E-46 bit: 186.0 Len: 158 Query Start:60 Query End:217 Subject Strand: POSITIVE Subject Start: 31 Subject End: 188 ...........................................................taacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... ...........................................................taaccaactcgttttcaacgctttacaaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagcggctgttttgcgctgcaaaacagccgctttcgcaacgccaaatcgaaattaccgtttttcgtcggaattatctttacaaaac.......................................................................................................................................... Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 3.0E-40 bit: 167.0 Len: 104 Query Start:80 Query End:183 Subject Strand: NEGATIVE Subject Start: 177 Subject End: 280 ...............................................................................ctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaa............................................................................................................................................................................ ...............................................................................ctttgcaaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagcggctcttttgcaacgcaaaacctacgctttcgcaatgtcaaatcgaaa............................................................................................................................................................................ Subject: pint_PI1688_PI1689|possible GTPase:conserved hypothetical protein|POSITIVE:POSITIVE|[1603964,1604449]|486 HSP 1 e-value: 1.0E-39 bit: 165.0 Len: 131 Query Start:86 Query End:216 Subject Strand: POSITIVE Subject Start: 174 Subject End: 304 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaa........................................................................................................................................... .....................................................................................aaaacctattgttttgcattgtaaaagcgtaggttttgcacggtaaaagcggctcttttgcatcgcaaaacatacgctttcgcaacgtcaaatcgaaattatcattttccctccgaattatctttacaaaa........................................................................................................................................... Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 1.0E-39 bit: 165.0 Len: 131 Query Start:86 Query End:216 Subject Strand: NEGATIVE Subject Start: 741 Subject End: 871 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaa........................................................................................................................................... .....................................................................................aaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagcggctgttttgcgttgcaaaacctacgctttcgcaacgccaaatcgaaattatcatttttcttgcgaactttctttacaaaa........................................................................................................................................... Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 5.0E-39 bit: 163.0 Len: 134 Query Start:86 Query End:219 Subject Strand: POSITIVE Subject Start: 233 Subject End: 365 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ .....................................................................................aaaacctattgttttgcattccaaaagtgtaggttttgcacggtaaaagcggctgttttgcattgcaaaacctacgctttcgcaatgccaaatcgaaattatcgtttttc-aatgaattatctttacaaaacca........................................................................................................................................ Subject: pint_PI1909_PI1910|DNA gyrase, B subunit:hypothetical protein|POSITIVE:POSITIVE|[1855331,1855917]|587 HSP 1 e-value: 3.0E-37 bit: 157.0 Len: 107 Query Start:71 Query End:177 Subject Strand: POSITIVE Subject Start: 332 Subject End: 438 ......................................................................ttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ......................................................................ttatcaacgcattgcaaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagtggcgcttttgcatcgcaaaacctacgctttcgcaatgtcaaa.................................................................................................................................................................................. Subject: pint_PI1424_PI1425|probable peptidase family M48:conserved hypothetical protein|POSITIVE:POSITIVE|[1344105,1344627]|523 HSP 1 e-value: 7.0E-32 bit: 139.0 Len: 158 Query Start:60 Query End:217 Subject Strand: POSITIVE Subject Start: 81 Subject End: 238 ...........................................................taacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... ...........................................................taactaactcgttatcaacacattgcaaaacctattgttttgcattccaaaagcgtaggttttacacggcaaaagcggctgttttgcgttgcaaaacagccgctttcgcaatgccaaatcgaaattatcatttttcttacgaataatctttacaaaac.......................................................................................................................................... Subject: pint_PI1930_PI1931|conserved hypothetical protein; possible ankyrin repeat protein (eukaryotic-like):alpha glycan phosphorylase, maltodextrin phosphorylase|POSITIVE:POSITIVE|[1875115,1875595]|481 HSP 1 e-value: 5.0E-30 bit: 133.0 Len: 147 Query Start:71 Query End:217 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 258 ......................................................................ttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... ......................................................................ttatcaacgcattacaaaacctattgttttgcgttccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaagagccgctttcgcaacgtcaaatcgaaattaccatttttcaaagaaattatctttacaaaac.......................................................................................................................................... HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 164 Subject End: 198 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI0195_PI0196|epimerase/reductase:hypothetical protein|POSITIVE:POSITIVE|[188263,188574]|312 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 102 Query Start:82 Query End:183 Subject Strand: NEGATIVE Subject Start: 178 Subject End: 279 .................................................................................ttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaa............................................................................................................................................................................ .................................................................................ttacaaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagcggctgttttgcgatgcaaaacagccgctttcgcaatgccaaatcgaaa............................................................................................................................................................................ Subject: pint_PI1728_PI1729|ATP-dependent Clp protease, proteolytic subunit:FKBP-type peptidyl-prolyl cis-trans isomerase, trigger factor|POSITIVE:POSITIVE|[1647719,1648555]|837 HSP 1 e-value: 7.0E-29 bit: 129.0 Len: 109 Query Start:86 Query End:194 Subject Strand: POSITIVE Subject Start: 416 Subject End: 524 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttc................................................................................................................................................................. .....................................................................................aaaacctattgtttcgcattgtaaaagagccgcttttgcacggtaaaagcggctcttttgccttgcaaaacctacgcttttgcaacgtcaaatcgaaattaccgttttc................................................................................................................................................................. Subject: pint_PI0652_PI0653|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[595853,596185]|333 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 158 Query Start:60 Query End:217 Subject Strand: NEGATIVE Subject Start: 146 Subject End: 303 ...........................................................taacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... ...........................................................taacaatctcatcatcagcgcattacaaaacctattgttttgcattccaaaagcgtaggttttgcaaggcaaaagagccgcttttggacggtaaaacctacactttcgcagtgccaaatcgaaattatcgtttttcttcagaattatctttacaaaac.......................................................................................................................................... HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:130 Query End:165 Subject Strand: POSITIVE Subject Start: 219 Subject End: 254 .................................................................................................................................aaaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. .................................................................................................................................aaaagcggctcttttgccttgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI0948_PI0949|hypothetical protein:endonuclease|POSITIVE:POSITIVE|[882039, 882403]|365 HSP 1 e-value: 4.0E-24 bit: 113.0 Len: 149 Query Start:71 Query End:219 Subject Strand: NEGATIVE Subject Start: 138 Subject End: 286 ......................................................................ttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ ......................................................................ttatcaacgcaatgcaaaacctattgttttgcattgtaaaagcgtaggttttacacggtcaaagcggctgttttgcgatgcaaaacagccgctttcgcaatgccaaatcgaaattatcattttccttacgaattatccttacaaaacca........................................................................................................................................ Subject: pint_PI0694_PI0695|Na+/dicarboxylate or sulfate symporter:xanthine/uracil permease family protein|POSITIVE:POSITIVE|[631238,631552]|315 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 148 Query Start:72 Query End:219 Subject Strand: NEGATIVE Subject Start: 85 Subject End: 232 .......................................................................tatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ .......................................................................tatcaacactttgcaaaacctattgttttgcattccaaaagcggctcttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgctttcgcagtgtcaaatcgaaattaccgttttccgtcggaattatctttacaaaacca........................................................................................................................................ HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 139 Subject End: 173 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI0574_PI0575|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[519505,519700]|196 HSP 1 e-value: 3.0E-22 bit: 107.0 Len: 146 Query Start:72 Query End:217 Subject Strand: NEGATIVE Subject Start: 9 Subject End: 154 .......................................................................tatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... .......................................................................tatcaacgcattacaaaacctattgttttgcattgcaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccactttcgcaacgtcaaatcgaaattatcatttttcttcggaattatctttacaaaac.......................................................................................................................................... Subject: pint_PI1563_PI1564|thiol:disulfide interchange protein dsbD:surface antigen BspA|POSITIVE:POSITIVE|[1482876,1483594]|719 HSP 1 e-value: 4.0E-21 bit: 103.0 Len: 112 Query Start:72 Query End:183 Subject Strand: POSITIVE Subject Start: 373 Subject End: 484 .......................................................................tatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaa............................................................................................................................................................................ .......................................................................tatcaacgcattacaaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaacagccgctttcgcaatgccaaatcgaaa............................................................................................................................................................................ Subject: pint_PI2088_PI2089|conserved hypothetical protein; possible PAP2 superfamily protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2045876,2046339]|464 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 131 Query Start:86 Query End:216 Subject Strand: NEGATIVE Subject Start: 155 Subject End: 285 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaa........................................................................................................................................... .....................................................................................aaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaacagcagctttcgcaacgtcaaatcgaaattatcgttttcttaacgaattatctttacaaaa........................................................................................................................................... Subject: pint_PI1051_PI1052|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[970289,970631]|343 HSP 1 e-value: 6.0E-20 bit: 99.6 Len: 154 Query Start:66 Query End:219 Subject Strand: POSITIVE Subject Start: 21 Subject End: 174 .................................................................actcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ .................................................................actctttatcaatgctttacaaaacctattgttttgcattccaaaagcggctcttttgcacggtaaaagcgtaggttttgcgttgtaaaagagccgctttcgcaacgtcaaaacgaaattattgtttttcatcagaattatctttacaaaacca........................................................................................................................................ Subject: pint_PI1174_PI1175|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1097103,1097511]|409 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 121 Query Start:57 Query End:177 Subject Strand: NEGATIVE Subject Start: 140 Subject End: 260 ........................................................gtgtaacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ........................................................gtgtaacttacttgttatcaacgcattgcaaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgctttcgcaacgtcaaa.................................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 152 Subject End: 186 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI0051_PI0054|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[53449,54718]|1270 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 125 Query Start:70 Query End:194 Subject Strand: POSITIVE Subject Start: 72 Subject End: 196 .....................................................................gttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttc................................................................................................................................................................. .....................................................................gttatcaacgtgttgcaaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaagagccgctttcgcaatgtcaaatcgaaattaccattttc................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: NEGATIVE Subject Start: 133 Subject End: 167 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI0023_PI0024|internalin-related protein:hypothetical protein|POSITIVE:POSITIVE|[28131,28517]|387 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 133 Query Start:86 Query End:218 Subject Strand: NEGATIVE Subject Start: 213 Subject End: 345 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacc......................................................................................................................................... .....................................................................................aaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaacagccgctttcgcaatgccaaatcgaaattatcgtttttcagcgaaattatctttacaaaacc......................................................................................................................................... Subject: pint_PI1029_PI1030|seryl-tRNA synthetase:biotin carboxyl carrier protein|POSITIVE:POSITIVE|[947266,947985]|720 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 108 Query Start:70 Query End:177 Subject Strand: NEGATIVE Subject Start: 348 Subject End: 455 .....................................................................gttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. .....................................................................gttatcaacgcattacaagacctattgttttgcgttccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaagagccgctttcgcaacgccaaa.................................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 360 Subject End: 394 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI1878_PI1879|hypothetical protein:uracil phosphoribosyltransferase|POSITIVE:POSITIVE|[1820222, 1820810]|589 HSP 1 e-value: 4.0E-18 bit: 93.7 Len: 107 Query Start:71 Query End:177 Subject Strand: POSITIVE Subject Start: 137 Subject End: 243 ......................................................................ttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ......................................................................ttatcaacgccttacaaaacctattgttttacgttccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaagagccgctttcgcaacgccaaa.................................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: NEGATIVE Subject Start: 197 Subject End: 231 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI2178_PI0001|1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, GcpE:possible Ton-B receptor|POSITIVE:POSITIVE|[1,539]|539 HSP 1 e-value: 6.0E-17 bit: 89.7 Len: 105 Query Start:73 Query End:177 Subject Strand: NEGATIVE Subject Start: 150 Subject End: 254 ........................................................................atcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ........................................................................atcaacgcattataaaacctattgttttgcgttccaaaagcggctgttttgcacggtaaaagcgtaggttttgcatcgcaaaagagccgctttcgcaacgccaaa.................................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 162 Subject End: 196 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgatgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI1577_PI1578|conserved hypothetical protein:rod shape-determining protein|POSITIVE:POSITIVE|[1503940,1504327]|388 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 128 Query Start:86 Query End:213 Subject Strand: NEGATIVE Subject Start: 72 Subject End: 199 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttaca.............................................................................................................................................. .....................................................................................aaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcagcgcaaaagagccgctttcgcaacgtcaaatcgaaattatagttttcttaatgaattatctttaca.............................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 120 Subject End: 154 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcgctgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI2056_PI2057|conserved hypothetical protein:conserved hypothetical protein; possible metal-dependent membrane protease|POSITIVE:POSITIVE|[2014489,2015078]|590 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 135 Query Start:82 Query End:216 Subject Strand: NEGATIVE Subject Start: 341 Subject End: 475 .................................................................................ttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaa........................................................................................................................................... .................................................................................ttacaaaacctattgtttcgcattccaaaagcggctgttttgcatggtaaaagcgtaagttttgcattacaaaagagccgctttcgcaacgtcaaatcgaaattattgttttcttaacgaattatctttacaaaa........................................................................................................................................... Subject: pint_PI2051_PI2052|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2007896,2008733]|838 HSP 1 e-value: 4.0E-15 bit: 83.8 Len: 138 Query Start:82 Query End:219 Subject Strand: POSITIVE Subject Start: 328 Subject End: 465 .................................................................................ttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ .................................................................................ttacaaaacctattgttttgcatcctaaaagcggctgttttgcacggtaaaagcataggttttgcgttgcaaaagagctgctttcgcaacgtcaaatcgaaattatcgttttcttaacgaattatctttacaaaacca........................................................................................................................................ Subject: pint_PI0770_PI0771|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[706415,706663]|249 HSP 1 e-value: 4.0E-15 bit: 83.8 Len: 118 Query Start:60 Query End:177 Subject Strand: POSITIVE Subject Start: 85 Subject End: 202 ...........................................................taacaaactcgttatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ...........................................................taacaaccttgttatcaatgtgttataaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgctttcgcaacgtcaaa.................................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: NEGATIVE Subject Start: 156 Subject End: 190 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................................................................................................................................................................. Subject: pint_PI2172_PI2174|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2114052,2114384]|333 HSP 1 e-value: 1.0E-14 bit: 81.8 Len: 105 Query Start:73 Query End:177 Subject Strand: POSITIVE Subject Start: 82 Subject End: 186 ........................................................................atcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaa.................................................................................................................................................................................. ........................................................................atcaacgtattacaaaacctattgttttgcgttccaaaagcggctcttttgcacggtaaaagcgtaggttttgcatcgtaaaagagccgctttcgcaacgtcaaa.................................................................................................................................................................................. Subject: pint_PI1583_PI1584|phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR)/IMP cyclohydrolase (ATIC):A/G-specific adenine glycosylase|POSITIVE:POSITIVE|[1511261,1512037]|777 HSP 1 e-value: 1.0E-14 bit: 81.8 Len: 148 Query Start:72 Query End:219 Subject Strand: POSITIVE Subject Start: 397 Subject End: 544 .......................................................................tatcaacgctttacaaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaata-ccgttttccttgtaaattatctttacaaaacca........................................................................................................................................ .......................................................................tatcaacgtgttacaaaacctattgttttgcattccaaaagcggctgttttgcacggcaaaagcgtaggttttacctcgcaaaacagccgctttcgcaacgtcaaaacgaaattatcaattttcaatgt-aattatctttacaaaacca........................................................................................................................................ Subject: pint_PI1803_PI1804|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1737852,1738248]|397 HSP 1 e-value: 6.0E-14 bit: 79.8 Len: 132 Query Start:86 Query End:217 Subject Strand: NEGATIVE Subject Start: 265 Subject End: 396 .....................................................................................aaaacctattgttttgcgttccaaaagcgtaggttttgcacggtaaaagcggctcttttgcattgcaaaacctacgctttcgcaacgtcaaatcgaaaataccgttttccttgtaaattatctttacaaaac.......................................................................................................................................... .....................................................................................aaaacctattgttttgcattgtaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaagagccgctttcgcaatgccaaatcgaaattaccgttttctttcggaataatctttacaaaac.......................................................................................................................................... HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:131 Query End:165 Subject Strand: POSITIVE Subject Start: 317 Subject End: 351 ..................................................................................................................................aaagcggctcttttgcattgcaaaacctacgcttt.............................................................................................................................................................................................. ..................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt.............................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGACAAGCUAAAAACAGGUGAUAAAUAUGUAAAGAUUUUUACAAACUCAUCUCUCGUGUAACAAACUCGUUAUCAACGCUUUACAAAACCUAUUGUUUUGCGUUCCAAAAGCGUAGGUUUUGCACGGUAAAAGCGGCUCUUUUGCAUUGCAAAACCUACGCUUUCGCAACGUCAAAUCGAAAAUACCGUUUUCCUUGUAAAUUAUCUUUACAAAACCAGGGUUAUUUCUUUGCUUUCUCAAGGUGAAAUGCAAGGGUGCAAUUUACUACAUUCCGCAUAUUCCAUCUUGCUUUUCGUAAGUGGCAGGAACGUGAUAAUUCAAGACCUUGCAUUGCUUUCAGAUGCUCCUUUUCA ..((.(((.....(((.(((........(((((((((.(((((((..........((((((((......)))))..)))..............(((.((((((((.(.(((((((((((((((((..(((((((.....)))))))..))))))))))))))))).).)))).)))).)))..............)))))))..)))))))))..)))...)))........((..(((.(((((..(((((((((.((.((((...(((.((((.......(((.(((((.....))))))))..)))).)))..))))))...)))))))))))))).))).))..))).)). (-96.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAGGAGCAUCUGAAAGCAAUGCAAGGUCUUGAAUUAUCACGUUCCUGCCACUUACGAAAAGCAAGAUGGAAUAUGCGGAAUGUAGUAAAUUGCACCCUUGCAUUUCACCUUGAGAAAGCAAAGAAAUAACCCUGGUUUUGUAAAGAUAAUUUACAAGGAAAACGGUAUUUUCGAUUUGACGUUGCGAAAGCGUAGGUUUUGCAAUGCAAAAGAGCCGCUUUUACCGUGCAAAACCUACGCUUUUGGAACGCAAAACAAUAGGUUUUGUAAAGCGUUGAUAACGAGUUUGUUACACGAGAGAUGAGUUUGUAAAAAUCUUUACAUAUUUAUCACCUGUUUUUAGCUUGUCUA ......(((.((.(((((((((.(((((((...(((.((((.(((((((..((((((.(.....).))).))).......))))))).)))).)))...)))))))......((((....((((((......(((....(((((((((.....))))))))).....)))....(((..(..(((((.(((((((((((((((((((....(((((.....)))))....)))))))))))))))))))....(((((((.....)))))))..)))))..)...))).))))))...)))).(((((((.((((((....)))))).)))))))...)))))))))..))))). (-105.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 950 150 101 65 16 pint:PI1702|5end_hypothetical_protein_1620587..1620817_POSITIVE 937 160 111 230 181 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 906 140 91 55 6 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 880 139 91 163 115 pint:PI0211|5end_hypothetical_protein_204067..204297_POSITIVE 880 139 91 182 134 pint:PI0196|5end_hypothetical_protein_188435..188665_POSITIVE 867 170 121 113 64 pint:PI1760|5end_hypothetical_protein_1684413..1684643_POSITIVE 858 180 131 95 46 pint:PI0926|5end_hypothetical_protein_856824..857054_POSITIVE 856 150 101 192 143 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 856 150 101 65 16 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 825 139 91 116 68 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 787 146 101 47 2 pint:PI0659|5end_hypothetical_protein_603203..603433_POSITIVE 729 130 82 192 144 pint:PI0653|5end_hypothetical_protein_596046..596276_POSITIVE 717 130 82 172 124 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 702 136 91 224 179 pint:PI2024|5end_hypothetical_protein_1978804..1979034_POSITIVE 702 136 91 51 6 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 697 130 82 178 130 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 682 136 91 47 2 pint:PI1204|5end_hypothetical_protein_1124430..1124660_POSITIVE 682 136 91 198 153 pint:PI0887|5end_hypothetical_protein_817536..817766_POSITIVE 682 136 91 91 46 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 682 136 91 184 139 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 942 170 121 118 69 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 906 140 91 110 61 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 873 150 101 120 71 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 824 137 91 151 105 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 744 170 122 49 1 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 717 130 82 127 79 pint:PI0568|3end_hypothetical_protein_513131..513281_POSITIVE 717 130 82 51 3 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 706 158 111 151 106 pint:PI1761|3end_hypothetical_protein_1684543..1684693_POSITIVE 706 130 81 127 78 pint:PI0815|3end_hypothetical_protein_754677..754827_POSITIVE 698 136 91 145 100 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 697 130 82 127 79 pint:PI0081|3end_hypothetical_protein_80700..80850_POSITIVE 688 130 81 127 78 pint:PI0638|3end_hypothetical_protein_582402..582552_POSITIVE 688 130 81 122 73 pint:PI0637|3end_hypothetical_protein_582397..582547_POSITIVE 682 136 91 145 100 pint:PI1797|3end_hypothetical_protein_1731559..1731709_POSITIVE 682 136 91 145 100 pint:PI0885|3end_hypothetical_protein_817563..817713_POSITIVE 682 136 91 145 100 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 682 136 91 145 100 pint:PI0820|3end_hypothetical_protein_759980..760130_POSITIVE 682 136 91 84 39 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 682 136 91 133 88 pint:PI0095|3end_hypothetical_protein_91850..92000_POSITIVE 682 136 91 145 100 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE