Origin IGS: attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaaaaattatacccttggcgcacaatcgcctctttcctgcttgttcttttttctatgccattagggcatgcgcttatgattatcatggaaaagacgatgtctgaaagtgctgttcatactgcaggcttcttgttaggacttgttggtttgataatggttattgtgggtgtgttcgtgaaaggagacacacgacaaacactttggggcttcattggtggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gccaccaatgaagccccaaagtgtttgtcgtgtgtctcctttcacgaacacacccacaataaccattatcaaaccaacaagtcctaacaagaagcctgcagtatgaacagcactttcagacatcgtcttttccatgataatcataagcgcatgccctaatggcatagaaaaaagaacaagcaggaaagaggcgattgtgcgccaagggtataattttttagtttccatgttgatagtttaaattgtttattttaaaattttagtgtatttggattcacgttaaaggaaacgtatatatagtctgatttattgtgaatatttgcttaattcggttttttttcttttctttgcaaggagaatagataaaaacaggcaatgaataat Mask Tandem Repeat Region ================================================ attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaaaaattatacccttggcgcacaatcgcctctttcctgcttgttcttttttctatgccattagggcatgcgcttatgattatcatggaaaagacgatgtctgaaagtgctgttcatactgcaggcttcttgttaggacttgttggtttgataatggttattgtgggtgtgttcgtgaaaggagacacacgacaaacactttggggcttcattggtggc Find is-nt database================================================ Query_seq: PI1430:PI1431|PI1430:PI1431:subtilisin-like serine proteinase:conserved hypothetical protein:->->:1351835..1352218 384 attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaaaaattatacccttggcgcacaatcgcctctttcctgcttgttcttttttctatgccattagggcatgcgcttatgattatcatggaaaagacgatgtctgaaagtgctgttcatactgcaggcttcttgttaggacttgttggtttgataatggttattgtgggtgtgttcgtgaaaggagacacacgacaaacactttggggcttcattggtggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1430:PI1431|PI1430:PI1431:subtilisin-like serine proteinase:conserved hypothetical protein:->->:1351835..1352218 384 attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaaaaattatacccttggcgcacaatcgcctctttcctgcttgttcttttttctatgccattagggcatgcgcttatgattatcatggaaaagacgatgtctgaaagtgctgttcatactgcaggcttcttgttaggacttgttggtttgataatggttattgtgggtgtgttcgtgaaaggagacacacgacaaacactttggggcttcattggtggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1430:PI1431|PI1430:PI1431:subtilisin-like serine proteinase:conserved hypothetical protein:->->:1351835..1352218 384 attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaaaaattatacccttggcgcacaatcgcctctttcctgcttgttcttttttctatgccattagggcatgcgcttatgattatcatggaaaagacgatgtctgaaagtgctgttcatactgcaggcttcttgttaggacttgttggtttgataatggttattgtgggtgtgttcgtgaaaggagacacacgacaaacactttggggcttcattggtggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 169 Max: 384 Len: 216 Subject: UniRef90_Q64UH8 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis HSP 1 e-value: 6.0E-14 bit: 78.6 Len: 216 Query Start:169 Query End:384 Subject Strand: null Subject Start: 21 Subject End: 92 ........................................................................................................................................................................ K L Y P W R T I A S F L L V L F S M P L G H A L M I I M E K T M S E S A V H T A X X X X X X X X X X X X X X X X X X K G D T R Q T L W G F I G G ........................................................................................................................................................................ K R Y W W K A I A S F L M V L F T M P L G H A L M I I M E H V M N E T V L H Y S A F A M G A V G M V M V I I G V F A Q G D T R Q T L W G F F G G attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 9 End: 107 ........ M P V F I Y S P C K E K K K N R I K Q I F T I N Q T I Y T F P L T ..................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 171 End: 266 .......................................................................................................................................................................... M I P L A H N R L F P A C S F F Y A I R A C A Y D Y H G K D D V ...................................................................................................................... Protein_Len: 60 Strand: + Start: 151 End: 384 ...................................................................................................................................................... M N M E T K K L Y P W R T I A S F L L V L F S M P L G H A L M I I M E K T M S E S A V H T A G F L L G L V G L I M V I V Protein_Len: 32 Strand: - Start: 282 End: 377 ......................................................................................................................................................................................................................................................................................... V A P K K N P S T P K I I T I T P T N T F P S V R C V S Q P K M ....... Protein_Len: 39 Strand: - Start: 147 End: 263 .................................................................................................................................................. V I L M S V L F N Y G Q R V I A E K R S T R K E I G N P C A S I I I M S F V M ......................................................................................................................... attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 127 End: 156 ..............................................................................................................................GTTGATAGTTTAAATTGTTTATTTTAAAAT.................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1430:PI1431|PI1430:PI1431:subtilisin-like serine proteinase:conserved hypothetical protein:->->:1351835..1352218 384 attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Intra-Species Hit: Count: 1 Min: 1 Max: 168 Len: 168 Subject: pint_PI1430_PI1431|subtilisin-like serine proteinase:conserved hypothetical protein|POSITIVE:POSITIVE|[1351835,1352218]|384 HSP 1 e-value: 6.0E-76 bit: 285.0 Len: 168 Query Start:1 Query End:168 Subject Strand: POSITIVE Subject Start: 1 Subject End: 168 attattcattgcctgtttttatctattctccttgcaaagaaaagnnnnnnnnccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaa........................................................................................................................................................................................................................ attattcattgcctgtttttatctattctccttgcaaagaaaagaaaaaaaaccgaattaagcaaatattcacaataaatcagactatatatacgtttcctttaacgtgaatccaaatacactaaaattttaaaataaacaatttaaactatcaacatggaaactaaa........................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAUUCAUUGCCUGUUUUUAUCUAUUCUCCUUGCAAAGAAAAGAAAAAAAACCGAAUUAAGCAAAUAUUCACAAUAAAUCAGACUAUAUAUACGUUUCCUUUAACGUGAAUCCAAAUACACUAAAAUUUUAAAAUAAACAAUUUAAACUAUCAACAUGGAAACUAAA ........(((((.((((((.(((.((((........)))).)))..)))))).)......)))).............................((((((......(((..........)))......((((((........))))))..........)))))).... (-14.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAGUUUCCAUGUUGAUAGUUUAAAUUGUUUAUUUUAAAAUUUUAGUGUAUUUGGAUUCACGUUAAAGGAAACGUAUAUAUAGUCUGAUUUAUUGUGAAUAUUUGCUUAAUUCGGUUUUUUUUCUUUUCUUUGCAAGGAGAAUAGAUAAAAACAGGCAAUGAAUAAU ....((((((...((((((((((((((...(((...........)))...)))))))))...))))).))))))..............(((((((((((((.........)))).((((((..(((.((((((....)))))).))).))))))..)))))))))... (-29.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 236 36 6 230 196 pint:PI0230|5end_hypothetical_protein_228590..228820_POSITIVE 230 40 7 78 46 pint:PI0545|5end_sugar_kinase_496411..496641_POSITIVE 215 35 5 166 122 pint:PI0218|5end_conserved_hypothetical_protein_210770..211000_POSITIVE 212 58 12 137 88 pint:PI0868|5end_exodeoxyribonuclease_VII,_large_subunit_804201..804431_POSITIVE 210 56 11 167 126 pint:PI1223|5end_conserved_hypothetical_protein_1143537..1143767_POSITIVE 205 36 7 215 185 pint:PI0735|5end_ABC_transporter,_ATP-binding_/_permease_668793..669023_POSITIVE 204 45 8 144 96 pint:PI1769|5end_hypothetical_protein_1696507..1696737_POSITIVE 199 107 61 137 87 pint:PI0387|5end_hypothetical_protein_354631..354861_POSITIVE 197 45 10 36 1 pint:PI1598|5end_phosphate_ABC_transporter,_phosphate-binding_component_1526646..1526876_POSITIVE 193 110 61 153 100 pint:PI0913|5end_glycerate_dehydrogenase_842700..842930_POSITIVE 192 96 54 228 186 pint:PI0319|5end_conserved_hypothetical_protein_301750..301980_POSITIVE 188 50 18 163 133 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 187 44 3 120 85 pint:PI1402|5end_3-deoxy-7-phosphoheptulonate_synthase_1322935..1323165_POSITIVE 187 109 79 58 23 pint:PI1354|5end_UDP-N-acetylglucosamine_2-epimerase_1270900..1271130_POSITIVE 186 70 29 149 112 pint:PI0845|5end_peptidyl-dipeptidase_778272..778502_POSITIVE 185 37 5 185 147 pint:PI1771|5end_alpha-glucosidase_1699996..1700226_POSITIVE 184 115 81 53 26 pint:PI1201|5end_peptidyl-arginine_deiminase_1116598..1116828_POSITIVE 184 35 12 143 117 pint:PI1061|5end_conserved_hypothetical_protein_979649..979879_POSITIVE 183 47 10 171 127 pint:PI1536|5end_hypothetical_protein_1455555..1455785_POSITIVE 183 36 1 220 183 pint:PI0092|5end_regulatory_protein_RecX_89080..89310_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 215 35 5 110 66 pint:PI0217|3end_conserved_hypothetical_protein;_possible_lipoprotein_210794..210944_POSITIVE 213 34 2 50 21 pint:PI1629|3end_hypothetical_protein_1551966..1552116_POSITIVE 212 58 12 58 9 pint:PI0867|3end_hypothetical_protein_804202..804352_POSITIVE 209 39 5 118 84 pint:PI0215|3end_hypothetical_protein_209004..209154_POSITIVE 205 36 7 102 72 pint:PI0734|3end_ABC_transporter,_ATP-binding_/_permease_668760..668910_POSITIVE 199 107 61 66 16 pint:PI0386|3end_integrase_354640..354790_POSITIVE 195 46 8 126 90 pint:PI0010|3end_conserved_hypothetical_protein_11679..11829_POSITIVE 194 41 5 122 97 pint:PI0378|3end_conserved_hypothetical_protein;_possible_helicase_349539..349689_POSITIVE 193 110 61 65 12 pint:PI0912|3end_conserved_hypothetical_protein_842692..842842_POSITIVE 191 113 85 110 81 pint:PI1009|3end_cytochrome_c_nitrite_reductase,_large_subunit_930840..930990_POSITIVE 188 50 18 34 4 pint:PI0814|3end_conserved_hypothetical_protein_754426..754576_POSITIVE 188 34 1 104 68 pint:PI0743|3end_hypothetical_protein_673026..673176_POSITIVE 186 70 29 113 76 pint:PI0844|3end_cytidine/deoxycytidylate_deaminase_(dCMP_deaminase)_778316..778466_POSITIVE 185 37 5 107 69 pint:PI1770|3end_4-alpha-glucanotransferase_1699998..1700148_POSITIVE 184 35 12 143 117 pint:PI1060|3end_conserved_hypothetical_protein_979729..979879_POSITIVE 180 100 54 76 24 pint:PI1041|3end_hypothetical_protein_960675..960825_POSITIVE 179 121 81 42 4 pint:PI1028|3end_glutamate_synthase,_small_subunit_945851..946001_POSITIVE 178 124 83 106 59 pint:PI0331|3end_hypothetical_protein_312575..312725_POSITIVE 178 124 83 76 29 pint:PI0330|3end_hypothetical_protein_312545..312695_POSITIVE 177 65 28 81 50 pint:PI0738|3end_conserved_hypothetical_protein_671871..672021_POSITIVE