Origin IGS: atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cttttcttttcttttaaataccttggctgtcgtgtagcctttttctgtttattttggctgcgaaagtacgaactttttcgcaaaaaacgttgtctttgcttcttttttgtaaaaagaaatgttgtatagtttgtttatttaactaagaataaataattttgcagcataaaagttacaaaactgtaacggtaattataatttattaaatcataaaaat Mask Tandem Repeat Region ================================================ atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Find is-nt database================================================ Query_seq: PI1423:PI1424|PI1423:PI1424:hypothetical protein:probable peptidase family M48:->->:1343072..1343288 217 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI1423:PI1424|PI1423:PI1424:hypothetical protein:probable peptidase family M48:->->:1343072..1343288 217 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI1423:PI1424|PI1423:PI1424:hypothetical protein:probable peptidase family M48:->->:1343072..1343288 217 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 91 End: 201 .......................................................................................... M Q H F F L Q K R S K D N V F C E K V R T F A A K I N R K R L H D S Q G I ................ Protein_Len: 42 Strand: - Start: 75 End: 200 .......................................................................... N F L C V I C C K K K C F L L L S L T K Q S F T R V K A A L I F L F L S C S L W P M ................. atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI1423:PI1424|PI1423:PI1424:hypothetical protein:probable peptidase family M48:->->:1343072..1343288 217 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Intra-Species Hit: Count: 1 Min: 1 Max: 217 Len: 217 Subject: pint_PI1423_PI1424|hypothetical protein:probable peptidase family M48|POSITIVE:POSITIVE|[1343072,1343288]|217 HSP 1 e-value: 1.0E-120 bit: 430.0 Len: 217 Query Start:1 Query End:217 Subject Strand: POSITIVE Subject Start: 1 Subject End: 217 atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag atttttatgatttaataaattataattaccgttacagttttgtaacttttatgctgcaaaattatttattcttagttaaataaacaaactatacaacatttctttttacaaaaaagaagcaaagacaacgttttttgcgaaaaagttcgtactttcgcagccaaaataaacagaaaaaggctacacgacagccaaggtatttaaaagaaaagaaaag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUUAUGAUUUAAUAAAUUAUAAUUACCGUUACAGUUUUGUAACUUUUAUGCUGCAAAAUUAUUUAUUCUUAGUUAAAUAAACAAACUAUACAACAUUUCUUUUUACAAAAAAGAAGCAAAGACAACGUUUUUUGCGAAAAAGUUCGUACUUUCGCAGCCAAAAUAAACAGAAAAAGGCUACACGACAGCCAAGGUAUUUAAAAGAAAAGAAAAG ....((((((((.....)))))))).((((.......((((((...((((..(((((....(((((((........)))))))........((((((..(((((((.....)))))))(((((((......)))))))......))).))).....))))).))))...))))))...((((.......))))..)))).................. (-37.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUCUUUUCUUUUAAAUACCUUGGCUGUCGUGUAGCCUUUUUCUGUUUAUUUUGGCUGCGAAAGUACGAACUUUUUCGCAAAAAACGUUGUCUUUGCUUCUUUUUUGUAAAAAGAAAUGUUGUAUAGUUUGUUUAUUUAACUAAGAAUAAAUAAUUUUGCAGCAUAAAAGUUACAAAACUGUAACGGUAAUUAUAAUUUAUUAAAUCAUAAAAAU ..............(((.((((..(((((.....)))))......(((...(((((((((((((.((((((.((((((.((((((((.(..((....))..))))))))))))))).....))))))...((((((((((......)))))))))).)))))))).)))))...)))..........)))).)))...................... (-41.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 197 157 128 77 pint:PI0625|5end_possible_membrane_transport_protein_573104..573334_POSITIVE 290 70 26 109 52 pint:PI2125|5end_D-tyrosyl-tRNA(Tyr)_deacylase_2074450..2074680_POSITIVE 289 200 151 163 106 pint:PI0198|5end_hypothetical_protein_190719..190949_POSITIVE 279 204 161 161 102 pint:PI1620|5end__two-component_system_sensor_protein_1543956..1544186_POSITIVE 276 162 127 83 48 pint:PI1425|5end_conserved_hypothetical_protein_1344488..1344718_POSITIVE 276 165 121 219 173 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 275 166 121 192 145 pint:PI1295|5end_hypothetical_protein_1208636..1208866_POSITIVE 271 166 121 89 42 pint:PI1204|5end_hypothetical_protein_1124430..1124660_POSITIVE 268 166 130 46 5 pint:PI1294|5end_hypothetical_protein_1208490..1208720_POSITIVE 267 165 121 92 46 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 267 165 121 184 138 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 267 166 130 41 3 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 267 165 121 92 46 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 263 147 111 189 152 pint:PI0067|5end_hypothetical_protein_66273..66503_POSITIVE 262 165 121 219 173 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 262 165 121 92 46 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 262 165 121 70 24 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 262 165 121 225 179 pint:PI0024|5end_hypothetical_protein_28378..28608_POSITIVE 258 165 130 46 9 pint:PI2023|5end_hypothetical_protein_1978585..1978815_POSITIVE 251 165 121 92 46 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 160 119 61 22 pint:PI0624|3end_hypothetical_protein_574471..574621_POSITIVE 290 70 26 75 18 pint:PI2124|3end_excinuclease_ABC,_subunit_C_2074496..2074646_POSITIVE 279 204 161 143 84 pint:PI1619|3end_two-component_system_response_regulator_1544018..1544168_POSITIVE 262 199 159 127 84 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 245 165 121 147 101 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 245 165 121 56 10 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 245 208 179 86 57 pint:PI0203|3end_conserved_hypothetical_protein_196064..196214_POSITIVE 239 200 154 132 87 pint:PI1536|3end_hypothetical_protein_1455808..1455958_POSITIVE 238 190 145 127 93 pint:PI2025|3end_conserved_hypothetical_protein_1979452..1979602_POSITIVE 237 166 121 148 101 pint:PI0905|3end_hypothetical_protein_834697..834847_POSITIVE 235 160 112 144 95 pint:PI0523|3end_hypothetical_protein_479844..479994_POSITIVE 232 198 151 61 18 pint:PI0723|3end_conserved_hypothetical_protein_657379..657529_POSITIVE 232 161 136 53 22 pint:PI0342|3end_hypothetical_protein_318049..318199_POSITIVE 232 159 118 70 32 pint:PI0268|3end_lysozyme-related_protein_259790..259940_POSITIVE 228 198 157 51 13 pint:PI0428|3end_hypothetical_protein_396321..396471_POSITIVE 218 161 124 61 32 pint:PI0187|3end_hypothetical_protein_178211..178361_POSITIVE 217 165 121 125 79 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 216 170 128 55 1 pint:PI0529|3end_hypothetical_protein_483741..483891_POSITIVE 212 198 153 53 14 pint:PI1720|3end_conserved_hypothetical_protein_1637992..1638142_POSITIVE 212 197 156 50 4 pint:PI0929|3end_copper_homeostasis_protein_861048..861198_POSITIVE